Determination of Cholinesterase in Human Blood Using near Infrared Spectroscopy

1998 ◽  
Vol 6 (A) ◽  
pp. A279-A284 ◽  
Author(s):  
Gyula Domján ◽  
János Jákó ◽  
István Vályi-Nagy ◽  
Károly Kaffka

The level of cholinesterase in the human blood is a very good indicator of liver function. In this study we desrcibe the relationship between cholinesterase values obtained with routine laboratory methods and near infrared (NIR) spectral data of 72 individuals with a wide range of cholinesterase levels. NIR spectra were measured with a SPECTRALYZER 10–25 (PMC) computerised research analyser. The relationship of laboratory data and values of the second derivative of the log (1/ TF) spectra measured at different wavelengths was determined with multiple linear regression (MLR) analysis using three-term linear summation equations. A correlation coefficient ( r) of 0.89 and a standard error of calibration ( SEC) of 987 units L−1 (U/L) of the enzyme were obtained. Our results indicate that NIR measurements of cholinesterase in the human blood serum can be related to the analytical data obtained with routine laboratory methods. NIR spectroscopy is a rapid, accurate, and inexpensive method for determining various constituents in the human blood.

1994 ◽  
Vol 2 (2) ◽  
pp. 67-78 ◽  
Author(s):  
Gyula Domján ◽  
Károly J. Kaffka ◽  
János M. Jákó ◽  
István T. Vályi-Nagy

In the present study we describe the relationship between laboratory values obtained with routinely used laboratory analytical methods and near infrared (NIR) spectral data of 126 whole blood and 228 blood serum samples. Spectra were measured with a SPECTRALYZER 1025 (PMC) computerised research analyser. The relationship among laboratory data and values of the second derivative of the log (1/ R) spectra measured at different wavelengths was determined by multiple linear regression (MLR) using three and four term linear summation equations, principal component regression (PCR) and partial least-squares (PLS) regression methods. Along with examples for qualitative detection of protein and lipid in human sera, as well as distinction of albumin and globulin dissolved in physiological saline solution, we describe mathematical models and evaluate their performance for the determination of protein and beta-lipoprotein (β-LP) content of serum as well as oxygen saturation and carbon dioxide pressure in whole blood. Validation of our results yielded a standard error of performance (SEP) of 2.47 g L−1 for protein content and 0.79 TU for β-LP content in blood serum, whereas SEP values of 5.41% for oxygen saturation and 5.27 mm Hg for carbon dioxide pressure in whole blood were found. Our results presented in this preliminary study indicate that NIR measurements can be related to analytical data of whole blood and serum. NIR spectroscopy is a rapid, accurate, cost effective method for determining quality parameters of whole blood and serum and might be a promising new tool in the field of automated clinical laboratory analysis.


1992 ◽  
Vol 262 (6) ◽  
pp. S9 ◽  
Author(s):  
E Bowdan

Regulation of feeding is a fundamental element of homeostasis. This is reflected in the similarity of control mechanisms in a wide range of animals, including insects and humans. A close examination of feeding behavior can illuminate the physiological processes driving regulation. A simple, inexpensive method for recording fine details of feeding by caterpillars is described. Possible experiments, interpretation of the data, and the relationship of observations to the underlying physiology, are outlined.


1998 ◽  
Vol 44 (12) ◽  
pp. 1115-1136 ◽  
Author(s):  
M J Butler ◽  
A W Day

The relationship of polyketide melanogenesis molecular biology to that of nonmelanin-producing pathways in a wide range of fungi and other organisms is discussed. Analytical methods and fundamental properties of melanins are discussed and fungal melanin properties are compared with those of animal and bacterial melanins. The enzymatic degradation of melanins by lignin peroxidases is described.Key words: fungal melanin, polyketide melanin, DHN melanin, melanin degradation, melanin properties, melanin analysis.


2018 ◽  
Vol 2018 ◽  
pp. 1-11
Author(s):  
Anna Sandak ◽  
Jakub Sandak ◽  
Dominika Janiszewska ◽  
Salim Hiziroglu ◽  
Marta Petrillo ◽  
...  

The overall goal of this work was to develop a prototype expert system assisting quality control and traceability of particleboard panels on the production floor. Four different types of particleboards manufactured at the laboratory scale and in industrial plants were evaluated. The material differed in terms of panel type, composition, and adhesive system. NIR spectroscopy was employed as a pioneer tool for the development of a two-level expert system suitable for classification and traceability of investigated samples. A portable, commercially available NIR spectrometer was used for nondestructive measurements of particleboard panels. Twenty-five batches of particleboards, each containing at least three independent replicas, was used for the original system development and assessment of its performance. Four alternative chemometric methods (PLS-DA, kNN, SIMCA, and SVM) were used for spectroscopic data classification. The models were developed for panel recognition at two levels differing in terms of their generality. In the first stage, four among twenty-four tested combinations resulted in 100% correct classification. Discrimination precision with PLS-DA and SVMC was high (>99%), even without any spectra preprocessing. SNV preprocessed spectra and SVMC algorithm were used at the second stage for panel batch classification. Panels manufactured by two producers were 100% correctly classified, industrial panels produced by different manufacturing plants were classified with 98.9% success, and the experimental panels manufactured in the laboratory were classified with 63.7% success. Implementation of NIR spectroscopy for wood-based product traceability and quality control may have a great impact due to the high versatility of the production and wide range of particleboards utilization.


2020 ◽  
Vol 9 (1) ◽  
pp. 35-45
Author(s):  
Iwan Sunardi ◽  
Vini Wiratno Putri

The purpose of this study was to examine the effect of the trust of co-workers and proactive personalities on career satisfaction by exchanging leader-members as mediation on employees of bus assembly companies in the city of Semarang. Career satisfaction is the phase in which employees’ long-term career needs are aligned with what they get while working. Employees will always look for opportunities and trust in the organization and people who will help them in achieving career satisfaction. The sampling method uses a purposive sampling technique in the category of staff and foreman employees who have worked for more than five years with a sample of 160 employees. The analytical data in this study uses descriptive statistical test methods, instinctual tests include validity and reliability, and hypothesis testing. The tool used to test in this study uses SmartPLS 3.0. The results of this study, colleague trust cannot directly influence career satisfaction. However, it can be mediated by the exchange of leader members and produce significant influence. For further researchers, they can re-examine the relationship of coworkers’ trust with career satisfaction. And can expand the object of research or respondents under study.


1995 ◽  
Vol 49 (6) ◽  
pp. 765-772 ◽  
Author(s):  
M. S. Dhanoa ◽  
S. J. Lister ◽  
R. J. Barnes

Scale differences of individual near-infrared spectra are identified when set-independent standard normal variate (SNV) and de-trend (DT) transformations are applied in either SNV followed by DT or DT then SNV order. The relationship of set-dependent multiplicative scatter correction (MSC) to SNV is also referred to. A simple correction factor is proposed to convert derived spectra from one order to the other. It is suggested that the suitable order for the study of changes using difference spectra (when removing baselines) should be DT followed by SNV, which leads to all derived spectra on the scale of mean zero and variance equal to one. If baselines are identical, then SNV scale spectra can be used to calculate differences.


Author(s):  
W.S. Green

This introduction to the symposium Inclusive Design and Usability gives an overview of some of the issues and problems facing ergonomists and designers who attempt to provide access to current technologies for a wide range of user groups, particularly those who may be considered disadvantaged or handicapped. The relationship of ergonomics and design is raised.


Author(s):  
Joel Altman

This chapter examines the use of ekphrasis in early modern theatre, with particular emphasis on its effect on the stage and the relationship of ekphrastic speech to the ongoing action in which it is enunciated. It maps the parameters of ekphrasis on the early modern English stage by considering a few examples of the ways in which ekphrasis instantiates early modern theatricality. It also discusses the expressive potential of ekphrastic speech and its transmission to the listener as well as the ironic uses of ekphrasis as a mode of persuasion, whether directed to oneself, an on-stage auditor, off-stage auditors, or all three. It argues that ekphrasis creates nothing less than what it calls ‘the psyche of the play’ and explains how the unusually flexible capacity of the staged word allows it to be used for a wide range of theatrical techniques, including the usual sense of ‘word-painting’. Finally, it looks at William Shakespeare’s deployment of ekphrasis in his work such asHamlet.


2010 ◽  
Vol 33 (1) ◽  
pp. 4.1-4.32 ◽  
Author(s):  
Richard B. Baldauf ◽  
Robert B. Kaplan

Applied Linguistics is a diverse field, comprising a substantial number of sub-fields, sub-specialisations and related fields. To see that this is the case one need only examine the various hand- books and encyclopaedic references that have been published in the last ten years to see the wide range of topics that have been covered. As with many professional areas in academia, Applied Linguistics is organised around national organisations, with its international structure being a loose confederation known as the International Association of Applied Linguistics (AILA). Given these diverse academic and structural arrangements, it should not be surprising if academics within different national associations were to cluster around different interests within the field. This study explores the question of what emphases are found in various parts of the world in Applied Linguistics, and in particular, the relationship of Australian Applied Linguistics to international trends using a structural text analysis of abstracts related to Applied Linguistics as well as an historical review of the trends involved.


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