Effect of pre-analytical handling on haematological variables in minipigs

2001 ◽  
Vol 35 (2) ◽  
pp. 147-152 ◽  
Author(s):  
A. K. Olsen ◽  
E. M. Bladbjerg ◽  
A. L. Jensen ◽  
A. K. Hansen
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Storage Time ◽  
Storage Temperature ◽  
Dilution Effect ◽  
Blood Sampling ◽  
Blood Cell Count ◽  
Red Blood Cell Count ◽  
A Minor ◽  
And Storage

Pre-analytical handling may be an important determinant of haematological variables, if analysis is delayed. We investigated the effect of anticoagulants, i.e. tripotassium ethylenediamine-tetraacetic acid (EDTA) and citric acid, theophylline, adenosine, dipyridamole (CTAD), storage time (0.5, 1.5, 3.5, 5.5, 7.5, 25.5 and 27.5 h after blood sampling), and storage temperature (5°C and 20°C) on the variation in haemoglobin (HGB), red blood cell count (RBC), haematocrit (HCT ), white blood cell count (WBC), and platelet count (PLT) in minipigs. Medians of HGB, RBC, HCT, WBC and PLT were significantly higher in EDTA tubes than in CTAD tubes due to the dilution effect of the anticoagulant. We found a minor significant increase in HCT after 25.5 h in blood stored at 20°C, and at the same time a minor significant increase in WBC in EDTA tubes stored at 20°C. We found a significant decrease in PLT in blood stored at 5°C, especially in EDTA tubes. Minor variations were also observed in HGB and RBC. Our results indicate that PLT should only be measured in tubes placed at room temperature. If HCT or WBC analyses are to be performed on the day after blood sampling, the samples must be stored in a refrigerator until analysis. Our studies underline that time delay before analysis of haematological variables can cause increased variation, and should therefore be limited as far as possible in order to reduce the number of animals needed to make reliable conclusions.

Storage of blood samples at or above 33℃ leads to rapid appearance of appreciable systemic bias in platelet and mean corpuscular volume related parameters: an important pre-analytical factor in tropical conditions

2018 ◽  
Vol 48 (4) ◽  
pp. 334-339
Author(s):  
Ashish Jain ◽  
Sanchit Jain ◽  
Neha Singh ◽  
Sushant Kumar Meinia ◽  
Nilotpal Chowdhury
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Storage Temperature ◽  
Climatic Conditions ◽  
Analytical Control ◽  
Blood Cell Count ◽  
Blood Samples ◽  
Tropical Conditions ◽  
Red Blood Cell Count ◽  
The Stability

There is scant documentation of the stability of common haematological parameters on storage of blood samples under tropical conditions. K2EDTA samples in multiple vials were taken from 20 healthy blood donors, baseline readings taken, and stored at 33°C and 37°C. Readings from the stored vials were taken after 1, 3 and 6 h. The percent change against the baseline readings at each time point for each storage temperature was calculated. Platelet counts showed an unacceptable shift within 1 h at 37°C and 3 h at 33°C; red cell volume related parameters showed an unacceptable shift within 3 h at 37°C and 6 h at 33°C. Haemoglobin, red blood cell count, white blood cell count and mean corpuscular haemoglobin remained stable for 6 h at both temperatures. The unacceptable change for many parameters on storage at ≥33°C demonstrates the importance of ensuring pre-analytical control in regions experiencing such climatic conditions.

scholarly journals Effect of plateletpheresis on hematocrit, hemoglobin and erythrocyte count: Meta-analysis 1980–2018

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Alejandro Gil-Betacur ◽  
Carmen Yulieth Mantilla-Gutiérrez ◽  
Jaiberth Antonio Cardona-Arias
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Red Blood Cell ◽  
Meta Analysis ◽  
Erythrocyte Count ◽  
Blood Cell Count ◽  
Selection Processes ◽  
Negative Effect ◽  
Red Blood Cell Count ◽  
The Mean

AbstractThe effects of platelet donation by apheresis on different parameters of the erythrogram are still unclear. The objective was to meta-analyze the effect of plateletpheresis on hematocrit, hemoglobin, and erythrocyte count, with a systematic review with random effects meta-analysis of the mean difference. The PRISMA guidelines were considered, as well as 133 search strategies on four different databases. Reproducibility was guaranteed and methodological quality was evaluated. Heterogeneity was evaluated with Galbraith and DerSimonian-Laird’s, publication bias with a funnel plot and a Begg’s test, sensitivity analysis and a cumulative meta-analysis were also conducted. Eighteen (18) articles were included, 17 evaluated the effects on hematocrit in 2,564 donors; 13 on hemoglobin in 1,640 donors; and 4 on red blood cell count in 243 donors. A decrease of 2.26% (CI95% = 2.11–2.41) was observed in hematocrit, of 0.80 g/dL (CI95% = 0.75–0.86) in hemoglobin and −0.21 × 1012/L (CI95% = −0.13; −0.29) in red blood cell count. Plateletpheresis has a negative effect on the erythrogram parameters, explained by blood loss in the kits used for the procedure and cell lysis. Such evidence is relevant to secure the efficiency and safety of the procedure, improve selection processes or determine the number of donations that can be performed without affecting donors’ health.

Red blood cell count in short-term prediction of cardiovascular disease incidence in the Gubbio population Study

2002 ◽  
Vol 57 (3) ◽  
pp. 177-185 ◽  
Author(s):  
Paolo Emilio PUDDU ◽  
Mariapoala LANTI ◽  
Alessandro MENOTTI ◽  
Mario MANCINI ◽  
Alberto ZANCHETTI ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Blood Cell ◽  
Cell Count ◽  
Red Blood Cell ◽  
Disease Incidence ◽  
Blood Cell Count ◽  
Short Term ◽  
Red Blood Cell Count ◽  
Short Term Prediction ◽  
Cardiovascular Disease Incidence

scholarly journals A follistatin‐based molecule increases muscle and bone mass without affecting the red blood cell count in mice

2019 ◽  
Vol 33 (5) ◽  
pp. 6001-6010 ◽  
Author(s):  
Andreas Lodberg ◽  
Bram C. J. Eerden ◽  
Bianca Boers‐Sijmons ◽  
Jesper Skovhus Thomsen ◽  
Annemarie Brüel ◽  
...  
Keyword(s):  
Bone Mass ◽  
Blood Cell ◽  
Cell Count ◽  
Red Blood Cell ◽  
Blood Cell Count ◽  
Red Blood Cell Count

Blood Parameters in Natural Populations of Trichosurus Species (Marsupialia: Phalangeridae). I. Age, Sex and Seasonal Variation in T. Caninus and T. Vulpecula. Ii. Influence of Habitat and Population Strategies of T. Caninus and T. Vulpecula.

1979 ◽  
Vol 27 (6) ◽  
pp. 913 ◽  
Author(s):  
JL Barnett ◽  
RA How ◽  
WF Humphreys
Keyword(s):  
Body Weight ◽  
Seasonal Variation ◽  
Blood Cell ◽  
Cell Count ◽  
Red Blood Cell ◽  
Plasma Glucose ◽  
Haemoglobin Concentration ◽  
Blood Parameters ◽  
Blood Cell Count ◽  
Red Blood Cell Count

Age, sex and seasonal related changes in body weight and blood parameters were determined for T caninus and T. vulpecula, whose distribution overlapped in part of the study area. In T. caninus age-related changes occurred in body weight, mean plasma glucose and protein concentrations and haematocrit. Seasonal changes occurred in plasma glucose and protein concentrations, haemoglobin concentration and red blood cell count. There were sexual dimorphisms in haematocrit, haemoglobin concentration and red blood cell count in both species; the mean values were greater in males. T. vulpecula also exhibited a sexual dimorphism in body weight (males > females). The only seasonal change in T. vulpecula was in haemoglobin concentration. Comparison of the species as adults showed that T. caninus had higher mean levels of body weight, haematocrit and haemoglobin concentration, while plasma lipid concentration and red blood cell counts were greater in T vulpecula. These species differences may be a reflection of the diets of the two species. In addition the measured parameters showed greater seasonal variation in T. caninus, a K-selected species, than in T. vulpecula, which is more r-selected.

scholarly journals The Erythropoetin rs1617640 Gene Polymorphism Associates with Hemoglobin Levels, Hematocrit and Red Blood Cell Count in Patients with Peripheral Arterial Disease

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1305
Author(s):  
Wilfried Renner ◽  
Melanie Kaiser ◽  
Sebastian Khuen ◽  
Olivia Trummer ◽  
Harald Mangge ◽  
...  
Keyword(s):  
Peripheral Arterial Disease ◽  
Gene Polymorphism ◽  
Blood Cell ◽  
Cell Count ◽  
Red Blood Cell ◽  
Arterial Disease ◽  
Multivariate Linear Regression Analysis ◽  
Blood Cell Count ◽  
Red Blood Cell Count ◽  
Peripheral Arterial

Background: Erythropoietin has a pivotal role in erythropoiesis and angiogenesis. A common polymorphism (rs1617640, A > C) in the promoter of the erythropoietin gene (EPO) has been associated with erythropoietin expression and microvascular complications of diabetes. We aimed to analyze the potential role of this polymorphism in the pathogenesis of peripheral arterial disease (PAD). Methods: EPO genotypes and laboratory markers for erythropoiesis were determined in 945 patients with PAD. Results: The minor EPO rs1617640 C-allele was associated in an allele-dose-dependent manner with hemoglobin levels (p = 0.006), hematocrit (p = 0.029), and red blood cell count (p = 0.003). In a multivariate linear regression analysis including conventional risk factors diabetes, sex, and smoking, EPO genotypes were furthermore associated with age at onset of PAD symptoms (p = 0.009). Conclusions: The EPO rs1617640 gene polymorphism affects erythropoiesis, leads to an earlier onset of PAD, and is a potential biomarker for the pathogenesis of this disease.

Sign in / Sign up

Export Citation Format

Share Document