scholarly journals Applicability Evaluation of Nile Blue A Dye to Korean Termite (Reticulitermes speratus kyushuensis)

2020 ◽  
Vol 36 (5) ◽  
pp. 333-338
Author(s):  
Si Hyun Kim ◽  
Ik Gyun Im ◽  
Yong Jae Chung
Keyword(s):  
Nile Blue ◽  
Reticulitermes Speratus ◽  
Nile Blue A ◽  
Applicability Evaluation

scholarly journals Evidence for Dual Site Binding of Nile Blue A toward DNA: Spectroscopic, Thermodynamic, and Molecular Modeling Studies

ACS Omega ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 2613-2625
Author(s):  
Mukti Mohammad ◽  
Harun Al Rasid Gazi ◽  
Kumud Pandav ◽  
Prateek Pandya ◽  
Md. Maidul Islam
Keyword(s):  
Molecular Modeling ◽  
Nile Blue ◽  
Nile Blue A ◽  
Modeling Studies ◽  
Molecular Modeling Studies ◽  
Dual Site ◽  
Site Binding

On Colouring Epon-Embedded Tissue Sections with Sudan Black B or Nile Blue A for Light Microscopy

1963 ◽  
Vol s3-104 (65) ◽  
pp. 109-115
Author(s):  
S. M. McGEE-RUSSELL ◽  
N. B. SMALE
Keyword(s):  
Lipid Droplets ◽  
Nile Blue ◽  
Thin Sections ◽  
Nile Blue A ◽  
Sudan Black B ◽  
Glass Slides ◽  
Acetone Water ◽  
Negative Effect ◽  
Blue Background ◽  
Dark Blue

Sections of osmium-fixed tissues embedded in epon 812 colour with either Sudan black B or Nile blue A solutions to reveal a variety of detail by direct microscopy with normal apochromatic or semi-apochromatic objectives. The clarity of the coloration gives a picture fully comparable to that seen by phase-contrast microscopy. The plastic is not removed, 1-µ sections or thinner sections down to green or gold, are mounted on clean glass slides by drying down from 20% acetone/water after flattening on a hot plate. Colouring is carried out at room temperature in Sudan black B (saturated solution in 70% alcohol) for 1 to 2 h. The result is a reversed or negative effect, for the epon plastic takes the stain avidly, but dense elements of the tissue do not, and appear white against a blue background of stained plastic. Lipid droplets retain a capacity to colour, becoming dark blue to blue-black. Nile blue sulphate ( 1% aqueous solution colours thin sections of tissue in 1 to 2 h at 60° C, acting apparently as a basic dye on most cell elements, and also colours lipid droplets dark blue. After both techniques the sections are mounted in Farrants's medium.

Rapid quantification of polyhydroxyalkanoates (PHA) concentration in activated sludge with the fluorescent dye Nile blue A

2011 ◽  
Vol 64 (3) ◽  
pp. 747-753 ◽  
Author(s):  
M. Oshiki ◽  
H. Satoh ◽  
T. Mino
Keyword(s):  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Nile Blue ◽  
Maximum Level ◽  
Fluorescence Measurement ◽  
Batch Reactors ◽  
Floc Size ◽  
Nile Blue A ◽  
On Line ◽  
Rapid Quantification

The present study was conducted (1) to develop a rapid quantification method of polyhydroxyalkanoates (PHA) concentration in activated sludge by Nile blue A staining and fluorescence measurement and (2) to perform on-line monitoring of PHA concentrations in activated sludge. Activated sludge samples collected from laboratory scale sequencing batch reactors and full-scale wastewater treatment plants were stained with Nile blue A and their fluorescence intensities were determined. There was a high correlation (R2 > 0.97) between the fluorescence intensities of Nile blue A and PHA concentrations in activated sludge determined by gas chromatography. The Nile blue A staining and fluorescence measurement method allows us to determine PHA concentrations in activated sludge within only five minutes and up to 96 samples can be measured at once by using microplate reader. On-line monitoring of PHA concentrations in activated sludge was achieved by using a fluorometer equipped with a flow cell and the time point at which PHA concentration in activated sludge reached the maximum level could be identified. In addition, we examined the influence of pH, floc size and co-existing chemicals in activated sludge suspension on the fluorescence intensities of Nile blue A.

Kinetic determination of trace levels of selenium(IV) and total selenium by Nile Blue A/hydrogen peroxide method

1998 ◽  
Vol 128 (1-2) ◽  
pp. 43-48 ◽  
Author(s):  
Gordana A. Milovanović ◽  
Radivoj B. Petronijević ◽  
Mira M. Čakar
Keyword(s):  
Hydrogen Peroxide ◽  
Nile Blue ◽  
Kinetic Determination ◽  
Nile Blue A ◽  
Total Selenium ◽  
Peroxide Method

Evaluation of fipronil baits against Microtermes mycophagus (Blattodea: Termitidae)

2015 ◽  
Vol 148 (3) ◽  
pp. 343-352 ◽  
Author(s):  
Naeem Iqbal ◽  
Theodore A. Evans ◽  
Shafqat Saeed ◽  
Hafiz Azhar Ali Khan
Keyword(s):  
Active Ingredient ◽  
Nile Blue ◽  
Subterranean Termite ◽  
Foraging Activity ◽  
Nile Blue A ◽  
Toilet Paper ◽  
Bait Station ◽  
Feasible Alternative

AbstractWe evaluated the efficacy of fipronil baits in suppressing or eliminating field colonies of Microtermes mycophagus (Desneux) (Blattodea: Termitidae) an important subterranean termite pest in Pakistan. We tested two doses (10 and 30 ppm) of fipronil in toilet paper baits, chosen from laboratory repellency tests. We monitored four colonies for foraging activity for one month before baiting, and mapped foraging territories with termites marked with Nile Blue A and agonistic tests. Before the fipronil baits were installed there were averages of 782–1938 workers and soldiers per bait station in the four colonies. After baiting, the colonies were eliminated as there were no workers per bait station, whereas the control colony had an average of 1142 workers per bait station. The three possibly eliminated colonies consumed around 47 mg of fipronil formulation (4.7 mg active ingredient) in 45–90 days. Our results suggest that baits containing fipronil could provide an economical and feasible alternative for the management of M. mycophagus in structures and buildings in Pakistan.

The Use of Meldola Blue and Nile Blue a, for Distinguishing Dead From Living Nematodes

Nematologica ◽  
1974 ◽  
Vol 20 (3) ◽  
pp. 271-276 ◽  
Author(s):  
I.R. Ogiga ◽  
R.H. Estey
Keyword(s):  
Nile Blue ◽  
Nile Blue A ◽  
Meldola Blue

scholarly journals Ukuran Populasi Koloni dan Daya Jelajah Rayap Coptotermes Curvignathus Holmgren di Pertanaman Pala di Kecamatan Meukek Kabupaten Aceh Selatan

2017 ◽  
Vol 2 (1) ◽  
pp. 1-10
Author(s):  
Fachrizal Yusmar ◽  
Muhammad Sayuthi ◽  
Alfian Rusdy
Keyword(s):  
Environmental Factors ◽  
Population Size ◽  
Nile Blue ◽  
Neutral Red ◽  
Mark Recapture ◽  
Nile Blue A ◽  
The World ◽  
Termite Attack

Abstrak. Pala (M. fragans) merupakan salah satu komoditas rempah unggulan yang bernilai tinggi, sebagaimana Indonesia menjadi salah satu produsen terbesar di dunia (70 – 75 %). Saat ini dalam membudidayakan tanaman pala, petani mengalami kendala yang salah satunya disebabkan oleh serangan rayap. Penelitian ini bertujuan untuk mengetahui ukuran populasi koloni dan daya jelajah rayap C. curvignathus pada tanaman pala (M. fragans) di kecamatan Meukek dengan menggunakan teknik triple mark recapture. Rayap C. curvignathus dikoleksi dari pertanaman pala (M. fragans) yang ditentukan dalam enam blok (I, II, III, IV, V, VI). Koleksi rayap diwarnai dengan neutral red 0,25% dan nile blue A 0,05% di laboratorium . Hasil pengamatan menunjukan bahwa Total ukuran populasi koloni sebesar 347719.06 individu dari 23 koloni yang tersebar dalam luas areal 7325 m². Rata-rata daya jelajah maksimum Rayap C. curvignathus sejauh 35 m yang diduga pengaruh dari beberapa faktor lingkungan terutama makanan (mengandung selulosa) yang tersedia sangat terbatas.Abstract. Nutmeg (M. Fragans) is one of commodity of superior spices which has high value, as Indonesia became one of the largest manufacturers in the world (70-75%).  Currently in cultivate a nutmeg crop, farmers faced some obstacles and termite attack is one of causes. The purpose of this research is to know the population size of colony and cruising termites C.Curvignathus on nutmeg crop (M. Fragans) in Meukek, South Aceh by using triple mark recapture technique. Termites C. Curvignathus collected from nutmeg crop (M. Fragans) which determined in six block (I, II, III, IV, V, and VI), the termites collection colored with neutral red 0.25% and nile blue A 0.05% in laboratory. The result of observation shows that total of population size of colony is 347.719,06 individual from 23 colonies scattered in the area of 7.325 m2.  The average of cruising termites C.Curnignathus as far as 35 m that suspected influence of some environmental factors especially a food (containing cellulose) very limited available.

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