Preparation of Molecularly Imprinted Technique and its Application in Sample Pretreatment

2021 ◽  
Vol 09 (01) ◽  
pp. 1-8
Author(s):  
毅 张
Keyword(s):  
Sample Pretreatment ◽  
Molecularly Imprinted

scholarly journals Preparation of Fluorescent Molecularly Imprinted Polymers via Pickering Emulsion Interfaces and the Application for Visual Sensing Analysis of Listeria Monocytogenes

Polymers ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 984 ◽  
Author(s):  
Xiaolei Zhao ◽  
Yan Cui ◽  
Junping Wang ◽  
Junying Wang
Keyword(s):  
Listeria Monocytogenes ◽  
Sample Pretreatment ◽  
Spherical Shape ◽  
Pickering Emulsion ◽  
Food Samples ◽  
Water Soluble ◽  
Water Emulsion ◽  
Molecularly Imprinted ◽  
Oil In Water ◽  
Oil In Water Emulsion

In this work, a novel molecularly imprinted polymer (MIP) with water-soluble CdTe quantum dots (QDs) was synthesized by oil-in-water Pickering emulsion polymerization using whole Listeria monocytogenes as the template. Listeria monocytogenes was first treated by acryloyl-functionalized chitosan with QDs to form a bacteria–chitosan network as the water phase. This was then stabilized in an oil-in-water emulsion comprising a cross-linker, monomer, and initiator, causing recognition sites on the surface of microspheres embedded with CdTe QDs. The resulting MIP microspheres enabled selective capture of the target bacteria via recognition cavities. The target bacteria Listeria monocytogenes was detected. Scanning electron microscopy (SEM) characterization showed that the MIPs had a rough spherical shape. There was visual fluorescence detection via quenching in the presence of the target molecule, which offered qualitative detection of Listeria monocytogenes in milk and pork samples. The developed method simplified the analysis process and did not require any sample pretreatment. In addition, the fluorescence sensor provided an effective, fast, and convenient method for Listeria monocytogenes detection in food samples.

The Recent Advance of Molecularly Imprinted on-Line Solid Phase Extraction and its Application in Sample Pretreatment - A Mini Review

2011 ◽  
Vol 415-417 ◽  
pp. 1799-1805 ◽  
Author(s):  
Qing Sun ◽  
Zhi Xiang Xu ◽  
Li Min Zhang ◽  
Lei Xu ◽  
Jie Zhou
Keyword(s):  
Solid Phase Extraction ◽  
High Selectivity ◽  
Solid Phase ◽  
Sample Pretreatment ◽  
Phase Extraction ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Recognition Ability ◽  
Functionalized Materials ◽  
On Line

Solid phase extraction (SPE) is a sample pretreatment technique which is increasingly popular and widely used. However, most of the traditional SPE material has poor selectivity. Molecular imprinting is an emerging technology for the preparation of functionalized materials with molecular recognition ability. Using the imprinted polymers as new sorbent, a molecularly imprinted on-line solid phase extraction coupled with chromatography or other techniques has become one of the most interesting applications of MIP, which has not only the extraction efficiency of SPE but also the high selectivity of imprinted polymers. This paper focuses on the recent states, advantages and outlooks of molecularly imprinted on-line solid phase extraction in sample pretreatment. This mini review may promote the extensive application of MIP in food safety.

Selective sample pretreatment by molecularly imprinted polymer monolith for the analysis of fluoroquinolones from milk samples

2010 ◽  
Vol 1217 (14) ◽  
pp. 2075-2081 ◽  
Author(s):  
Ming-Ming Zheng ◽  
Rui Gong ◽  
Xing Zhao ◽  
Yu-Qi Feng
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Sample Pretreatment ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Milk Samples ◽  
Polymer Monolith

scholarly journals Molecularly Imprinted Nanomicrospheres as Matrix Solid-Phase Dispersant Combined with Gas Chromatography for Determination of Four Phosphorothioate Pesticides in Carrot and Yacon

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Mengchun Zhou ◽  
Nana Hu ◽  
Shaohua Shu ◽  
Mo Wang
Keyword(s):  
Gas Chromatography ◽  
Solid Phase ◽  
Organophosphorus Pesticides ◽  
Sample Pretreatment ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Selective Method ◽  
Dummy Template ◽  
Optimized Conditions

An efficient, rapid, and selective method for sample pretreatment, namely, molecularly imprinted matrix solid-phase dispersion (MI-MSPD) coupled with gas chromatography (GC), was developed for the rapid isolation of four phosphorothioate organophosphorus pesticides (tolclofos-methyl, phoxim, chlorpyrifos, and parathion-methyl) from carrot and yacon samples. New molecularly imprinted polymer nanomicrospheres were synthesized by using typical structural analogue tolclofos-methyl as a dummy template via surface grafting polymerization on nanosilica. Then, these four pesticides in carrot and yacon were extracted and adsorbed using the imprinted nanomicrospheres and further determined by gas chromatography. Under the optimized conditions, a good linearity of four pesticides was obtained in a range of 0.05–17.0 ng·g−1withRvarying from 0.9971 to 0.9996, and the detection limit of the method was 0.012~0.026 ng·g−1in carrot and yacon samples. The recovery rates at two spiked levels were in the range of 85.4–105.6% with RSD ≤9.6%. The presented MI-MSPD method combined the advantages of MSPD for allowing the extraction, dispersion, and homogenization in two steps and the advantages of MIPs for high affinity and selectivity towards four phosphorothioate pesticides, which could be applied to the determination of pesticide residues in complicated vegetal samples.

Synthesis and application of zwitterionic magnetic molecularly imprinted polymer for selective removal of fluoroquinolones from aqueous solution

2020 ◽  
Vol 16 ◽  
Author(s):  
Huikai Shao ◽  
Samar Douh Cherif ◽  
Jincai Wang ◽  
Qiqin Wang ◽  
Zhengjin Jiang
Keyword(s):  
Contact Angle ◽  
Sample Pretreatment ◽  
Ammonium Hydroxide ◽  
Infrared Spectrometry ◽  
X Ray Diffraction ◽  
Water Contact ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Langmuir Isotherm Model ◽  
Pseudo Second Order

Background: Although molecularly imprinted polymers (MIPs) have now been widely utilized for the sample pretreatment, chromatography separation and removal of pollutants, conventional MIPs are still lack of satisfactory compatibility with water, thus leading to poor molecular recognition for target analytes in aqueous solutions. This aim of study was to prepare the watercompatible MIPs. Method: The zwitterionic MMIP was synthesized on vinyltrimethoxysilane coated Fe3O4 (Fe3O4-VTMS) through a surface imprinting process by using levofloxacin (LEV) as template, [2-(methacryloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide (SPE) as functional monomer, N, N-methylene-bis-acrylamide (MBA) as crosslinker and potassium persulfate as initiator. Results: The prepared zwitterionic LEV-MMIPs were characterized by fourier transform infrared spectrometry, X-ray diffraction, vibrating sample magnetometry, scanning electron microscopy and water contact angle test. The characterization results showed that the zwitterionic LEV-MMIPs exhibited good magnetic responsiveness and super-hydrophilicity (the contact angle is 0o ). The optimum pH for binding LEV was found to be 6.0 and the binding isotherm followed Langmuir isotherm model of monolayer adsorption. Binding process was very fast and pseudo-second-order model fitted well with the kinetic data. Adsorption capacity of this zwitterionic MMIPs was 54.30 mg g-1 with a selectivity factor of 1.6 compared to the magnetic non-imprinted polymers (MNIPs). Its feasibility of removing fluoroquinolones (FQs) from environmental sample was demonstrated using pearl river water spiked with LEV, gatifloxacin, ciprofloxacin and lomefloxacin. The zwitterionic LEV-MMIPs could be reused at least five times with a removal efficiency of more than 80% for the selected FQs. Conclusion: The prepared zwitterionic LEV-MMIP is a promising sorbent for rapid, selective and efficient removal of fluoroquinolones from aqueous medium.

Selective and automated sample pretreatment by molecularly imprinted polymer for the analysis of the basic drug alfuzosin from plasma

2008 ◽  
Vol 1196-1197 ◽  
pp. 73-80 ◽  
Author(s):  
F. Hugon-Chapuis ◽  
J.U. Mullot ◽  
G. Tuffal ◽  
M.-C. Hennion ◽  
V. Pichon
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Sample Pretreatment ◽  
Imprinted Polymer ◽  
Basic Drug ◽  
Molecularly Imprinted

Mixed-Template Molecularly Imprinted Technique and its Application Research

2013 ◽  
Vol 787 ◽  
pp. 99-105 ◽  
Author(s):  
Zhi Gang Xu ◽  
Zhi Min Liu ◽  
Yun Li Chen
Keyword(s):  
Solid Phase Extraction ◽  
Molecularly Imprinted Polymers ◽  
Solid Phase ◽  
Sample Pretreatment ◽  
Recent Decade ◽  
Phase Extraction ◽  
Application Research ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Mixed Template

Molecularly imprinted techniques have been rapidly developed in recent decade. Some novel molecularly imprinted techniques and some novel application forms of molecularly imprinted polymers have been developed. And it has been widely used in sample pretreatment. In this paper, novel mixed-template molecularly imprinted techniques are introduced. The development and applications of mixed-template molecularly imprinted techniques in recent decade are reviewed, including dual-template molecularly imprinted technique and multi-template molecularly imprinted technique, and its application in chromatographic solid phase, solid phase extraction and microsphere extraction. Moreover, the trends of mixed-template molecularly imprinted techniques in sample pretreatment are prospected.

scholarly journals Magnetic Molecularly Imprinted Polymers: Synthesis and Applications in the Selective Extraction of Antibiotics

2021 ◽  
Vol 9 ◽  
Author(s):  
Junyu Li ◽  
Yiran Wang ◽  
Xiuxia Yu
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Bacterial Resistance ◽  
Sample Pretreatment ◽  
Selective Extraction ◽  
Future Research ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Magnetic Molecularly Imprinted Polymers ◽  
Novel Material ◽  
Antibiotics Residues

Recently, magnetic molecularly imprinted polymers (MMIPs) have integrated molecular imprinting technology (MIT) and magnetic separation technology and become a novel material with specific recognition and effective separation of target molecules. Based on their special function, they can be widely used to detect contaminants such as antibiotics. The antibiotic residues in the environment not only cause harm to the balance of the ecosystem but also induce bacterial resistance to specific antibiotics. Given the above consideration, it is especially important to develop sensitive and selective methods for measuring antibiotics in the complex matrix. The combination of MMIPs and conventional analytical methods provides a rapid approach to separate and determine antibiotics residues. This article gives a systematic overview of synthetic approaches of the novel MMIPs materials, briefly introduces their use in sample pretreatment prior to antibiotic detection, and provides a perspective for future research.

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