scholarly journals Serological survey of antibodies against Toxoplasma gondii in organic sheep and goat farms in Greece

2017 ◽  
Vol 58 (1) ◽  
pp. 22 ◽  
Author(s):  
V. NTAFIS (Β. ΝΤΑΦΗΣ) ◽  
E. XYLOURI (Ε.Μ. ΞΥΛΟΥΡΗ) ◽  
A. DIAKOU (Α. ΔΙΑΚΟΥ) ◽  
K. SOTIRAKOGLOU (Α. Κ. ΣΩΤΗΡΑΚΟΓΛΟΥ) ◽  
I. KRITIKOS (Ι. ΚΡΗΤΙΚΟΣ) ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Parasitic Infection ◽  
Basic Research ◽  
Reproductive Period ◽  
Enzyme Linked Immunosorbent Assay ◽  
Organic Farms ◽  
Blood Samples ◽  
Significant Difference ◽  
Female Sheep

Toxoplasmosis is a parasitic infection with global distribution caused by the protozoon Toxoplasma gondii. The present study is divided into the preliminary and the basic research. In the preliminary research, 413 serum samples (184 sheep and 229 goats) from organic farms of Péloponnèse and Western Sterea Hellas were tested for IgG antibodies against Τ gondii, by using enzyme-linked immunosorbent assay (ELISA). Blood sampling took place during spring of 2005 and samples were taken from male animals and female that had aborted during the reproductive period of 2004-2005. Significant prevalence of antibodies was the reason that further research of toxoplasmosis in organic farms was conducted. For the basic research, small ruminants' blood samples from organic farms of Péloponnèse, Western Sterea Hellas and the county of Ioannina, were collected during summer of 2006. Blood samples were collected from flocks with cases of abortions during the reproductive period of 2005-2006. Animals were separated into 3 groups: males, females that had abortions during the reproductive period of 2005-2006 and females that did not have abortions during the same period. A total number of 182 sheep sera and 167 goat sera were tested by using the enzyme-linked immunosorbent assay (ELISA). The results showed that antibodies were detected in 38.46% of male sheep, 60.87% of female sheep that had abortions during the reproductive period 2005-2006 and in 54.21% of female sheep that did not have abortions during the same period. Significant difference was determined only between male and female animals (p<0.05). In addition, antibodies were detected in 12.12% of male goats, 14.29% of female goats that had abortions and in 22.35% of female goats that did not have abortions in the reproductive period 2005-2006. There was no significant difference between the results (p>0.05). Seroprevalence in goats was significandy lower than that of the sheep (p<0.05). In animals, aged less than 4 years old, seroprevalence was significantly lower (39.58% for sheep and 13.04% for goats) than that for animals aged more than 4 years old (61.97% for sheep and 24% for goats) (p<0.05). The results of the survey suggest that there is high prevalence of toxoplasmosis both in animals that had aborted and in animals that had not aborted and that there are different species and gender susceptibility. Results also indicate that toxoplasmosis is more common in older animals.

Seroepidemiology of Toxoplasma gondii in pet dogs and cats in Beijing, China

2008 ◽  
Vol 53 (3) ◽  
Author(s):  
Jinhai Yu ◽  
Jun Ding ◽  
Zhaofei Xia ◽  
Degui Lin ◽  
Yili Li ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Age Groups ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pet Dogs ◽  
Significant Difference ◽  
Beijing China

AbstractSera from 534 pet dogs and 335 pet cats from Beijing (China) were tested for anti-Toxoplasma gondii antibodies using an enzyme-linked immunosorbent assay or the latex agglutination test. The seropositivity by year, season, sex and age was analysed. Overall, 128 dogs (24.0%) and 50 cats (14.9%) had antibodies to T. gondii. When analysed by season, the highest seroprevalence was found in spring for dogs (31.3%) and cats (25.1%), and the differences in seroprevalence by season was statistically significant in cats (P<0.01) but not in dogs. The seroprevalence in male dogs (23.7%) and cats (15.1%) were slightly higher than their female counterparts (18.0% in dogs and 12.3% in cats). There was no obvious pattern of seropositivity or significant difference in different age groups in dogs or cats; nonetheless, a high proportion of dogs at 4 years of age were positive to T. gondii (31.8%) while cats with relatively high seropositivity rates were at 1 or 3.4 years of age (13.14%).

scholarly journals Serological diagnosis of Toxoplasmosis by specific antibody in camels of Wasit province, Iraq

2018 ◽  
Vol 1 (29) ◽  
pp. 412-423
Author(s):  
Basim Mohammed Hanon
Keyword(s):  
Public Health ◽  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Zoonotic Parasite

Background: toxoplasma gondii is a zoonotic parasite, more added a major public health is worldwide because have high distribution in livestock. Objectives: the main aim of this study determine the occurrence of the seroepidemiological toxoplasmosis in camels in waist province of Iraq from November 2016 to April 2017. Materials and Methods: blood samples collected of animals randomly were included six different groups of animals were diagnosed by A Latex agglutination test (LAT) and indirect enzyme linked immunosorbent assay (ELISA) kits.

scholarly journals An investigation of sheep toxoplasmosis in Basrah Province / Iraq

2010 ◽  
Vol 9 (3) ◽  
pp. 128
Author(s):  
K. M. Al-Saad ◽  
Saad Hashim Al-Husseiny
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Indirect Elisa ◽  
Elisa Test ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Study Results ◽  
Significant Difference ◽  
The Difference

The objective of this study was to investigate Toxoplasma gondii antibodies among sheep in different regions of Basrah province (including Al-Mdayna, Shatt Al-Arab, Al-Basrah, Al-Zubayr, and Abu Al-Khasib). The study was started in Oct. 2008 and was finished in May 2009, using latex agglutination test (LAT) and indirect enzyme linked immunosorbent assay (ELISA) IgG, 309 adult sheep were randomly selected from 15 herds among different ages and both sexes and used in this study, including 62 pregnant ewes, 185 non-pregnant ewes, 14 aborted ewes, and 48 rams. Results showed, that 60.84% were seropositive by LAT, whereas 51.11% were seropositive by ELISA IgG test, among animals used in this study, results detected that 79.03% pregnant ewes (highest value), 56.75% nonpregnant ewes,71.40% aborted ewes and 50% Rams (lowest value) were seropositive by LAT, whereas 56.52% pregnant ewes, 51.11% non-pregnant ewes, 83.33% aborted ewes (highest value), and 31.25% Rams (lowest value) were seropositive by indirect ELISA IgG. Moreover, among regions of Basrah province, the details of percentage of T.gondii antibodies were 54.54% in AL-Basrah , 71.43% in Abu Al-Khasib (highest value), 57.35% in Al- Mdayna, 47.83% in Shatt Al-Arab (lowest value), and 67.16% in Al-Zubayr by LAT, whereas 63.64% in AL-Basrah (highest value), 22.73% in Abu Al-Khasib (lowest value), 57.89% in Al-Mdayna, 50% in Shatt Al-Arab and 61.90% in Al-Zubayr by indirect ELISA test. Although the difference observed in the percentage of Toxoplasma gondii antibodies among different regions of Basrah, there was no significant difference P>0.05 detected LAT, whereas in the indirect ELISA IgG test there was significant difference P<0.05. Ewes showed high percentages 62.83%, 55.40% of toxoplasmosis than rams 50 %, 31.25% by LAT and ELISA test respectively. The highest titer was 1/4 28.57% were detected in pregnant ewes and lowest titers were 1/2, 1/8, and 1/256 0.0% were detected in aborted ewes and in ramsrespectively.

Comparison of LFCI, LAT and ELISA for the diagnosis of toxoplasmosis in goats

2015 ◽  
Author(s):  
Sadaf Niaz ◽  
Rafi Ullah ◽  
Bahrawar Said ◽  
Sumaira Shams ◽  
Naser M. AbdEl-Salam ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Lateral Flow ◽  
Latex Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Male And Female ◽  
Toxoplasma Gondii Infection

This study was carried out in order to investigate the seroprevalence of Toxoplasma gondii infection in goats of seven different regions of District Dir (Lower), Pakistan. Lateral Flow Chromatographic Immunoassay (LFCI), Latex Agglutination Test (LAT) and Enzyme-Linked Immunosorbent Assay (ELISA) were used for the detection of T. gondii infection in goats. A total of 175 blood samples were collected from goats. Out of which 70 (40%) were found positive for T. gondii. The prevalence of toxoplasmosis among goats in seven areas of District Dir (Lower) was found high at Samarbagh (48%), Balambat (44%) followed by khall (40%), Munda (40%), LalQilla (36%) and lowest in Adenzai and Timergara (36%). This study demonstrated that the prevalence in older goats was significantly (P< 0.05) higher (48.91%) than younger ones (30.12%). The seroprevalence in male and female goats were 16 (29%) and 54 (45%) respectively.

scholarly journals Efektifitas quad-helix dalam perawatan defisiensi transversal maksila pada anakEffectiveness of quad-helix for transverse maxillary deficiency treatment in children

2021 ◽  
Vol 33 (3) ◽  
pp. 180
Author(s):  
Stephanie Wiguna ◽  
Risti Saptarini Primarti ◽  
Iwan Ahmad Musnamirwan
Keyword(s):  
Immunosorbent Assay ◽  
Interleukin 6 ◽  
Venous Blood ◽  
Test Sample ◽  
Apical Periodontitis ◽  
T Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Significant Difference ◽  
Quad Helix

Pendahuluan: Periodontitis apikalis adalah peradangan dan kerusakan jaringan pada daerah apikal jaringan periodonsium. Terjadi akibat pulpa nekrosis mengalami perluasan infeksi dari bakteri saluran akar menuju apeks gigi. Penelitian terdahulu membuktikan bahwa periodontitis apikalis dapat dikaitkan dengan peningkatan kadar interleukin-6. Interleukin-6 adalah salah satu sitokin pro-inflamatori yang mempunyai peran penting dalam respon inflamasi. Tujuan penelitian menganalisis perbedaan kadar interleukin-6 dalam darah vena antara pasien dengan periodontitis apikalis dan pasien tanpa periodontitis apikalis. Metode: Jenis penelitian observasional analitik dengan dua puluh sampel stok darah vena yang terdiri atas 10 sampel darah vena pasien dengan periodontitis apikalis dan 10 sampel darah vena pasien tanpa periodontitis apikalis. Serum darah yang telah dipisahkan dengan metode sentrifugasi dipakai sebagai sampel uji enzyme-linked immunosorbent assay (ELISA) sandwich untuk mengukur kadar IL-6 dengan membaca nilai absorbansi dan kurva standar. Data yang diperoleh dianalisis menggunakan uji Shapiro-Wilk, dilanjutkan dengan uji T tidak berpasangan. Hasil: Kadar IL-6 teridentifikasi pada semua sampel. Sampel darah vena pasien tanpa periodontitis apikalis memiliki kadar IL-6 berkisar antara 4,7-18,74 mg/L, sedangkan kadar IL-6 pada pasien dengan periodontitis apikalis 4,0-90,75 mg/L. Uji T tidak berpasangan menunjukan bahwa terdapat perbedaan yang bermakna (p=0.02). Simpulan:  Kadar IL-6 pada darah vena dengan periodontitis apikalis lebih tinggi dibandingkan kadar IL-6 pada tanpa periodontitis apikalis.Kata kunci: periodontitis apikalis; interleukin-6; Enzim-Linked Immunosorbent Assay Sandwich ABSTRACT Introduction: Apical periodontitis is inflammation and tissue damage in the apical area of the periodontium. Occurs due to pulp necrosis experiencing an expansion of infection from root canal bacteria to the apex of the tooth. Previous studies have shown that apical periodontitis can be associated with elevated levels of interleukin-6. Interleukin-6 is a pro-inflammatory cytokine that has a vital role in the inflammatory response. The study aimed to analyze differences in interleukin-6 levels in venous blood between patients with apical periodontitis and patients without apical periodontitis. Methods: This research was an analytic observational study with twenty venous blood samples consisting of 10 venous blood samples from patients with apical periodontitis and ten venous blood samples from patients without apical periodontitis. Blood serum that has been separated by centrifugation method was used as a sandwich enzyme-linked immunosorbent assay (ELISA) test sample to measure IL-6 levels by reading absorbance values and standard curves. The data obtained were analyzed using the Shapiro-Wilk test, followed by an unpaired T-test. Results: IL-6 levels were identified in all samples. Venous blood samples from patients without apical periodontitis had IL-6 levels ranging from 4.7-18.74 mg/L, while IL-6 levels in patients with apical periodontitis were 4.0-90.75 mg/L. The unpaired t-test showed that there was a significant difference (p=0.02). Conclusion: IL-6 levels in venous blood with apical periodontitis were higher than IL-6 levels in those without apical periodontitis.Keywords: apical periodontitis; interleukin-6; Enzyme-Linked Immunosorbent Assay Sandwich 

scholarly journals Prevalence of Toxoplasma gondii and Neospora caninum infections in households sheep "Elevage en case" in Dakar, Senegal

2019 ◽  
Vol 12 (7) ◽  
pp. 1028-1032 ◽  
Author(s):  
Laibané Dieudonné Dahourou ◽  
Oubri Bassa Gbati ◽  
Madi Savadogo ◽  
Bernadette Yougbare ◽  
Amadou Dicko ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Significant Variation ◽  
Neospora Caninum ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Sheep Breeding ◽  
Modified Agglutination Test ◽  
Husbandry System

Aim: The study aimed to evaluate the occurrence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in sheep breeding in a particular husbandry system called "Elevage en case" in Dakar, Senegal. Materials and Methods: Blood samples were collected from 278 sheep. Serum was harvested and used for analysis. For the detection of T. gondii antibodies, 278 sera were analyzed using the modified agglutination test, while the enzyme linked-immunosorbent assay was used on 174 sheep sera to look for N. caninum antibodies. Results: This study showed that 29.4±6.8% of sheep carried both T. gondii and N. caninum antibodies. The overall prevalence was 60.1±5.7% and 41.9±7.3% for toxoplasmosis and neosporosis, respectively. For toxoplasmosis, the prevalence was higher in Gueule Tapée (63.3%) than in Medina (58.9%), but the variation was not significant (p=0.45). Regarding the age of animals, the prevalence was significantly higher in animals over 2 years old compared to those under 2 years old (p=0.002). For neosporosis, the prevalence was significantly higher in Medina (48.67%) than Gueule Tapée (16.7%) (p=0.001), but non-significant variation was noted according to animal age. Conclusion: The study showed that sheep reared in households have carried antibodies of T. gondii and N. caninum. The prevalence was high and it means that consumption of meat from these animals is risky if the meat is eaten undercooked.

scholarly journals Presence of anti-Toxoplasma antibodies in humans and their cats in the urban zone of Guadalajara

1999 ◽  
Vol 32 (5) ◽  
pp. 483-488 ◽  
Author(s):  
María de la Luz Galván Ramírez ◽  
Guillermo Sánchez Vargas ◽  
Marcos Vielma Sandoval ◽  
Juan Luis Soto Mancilla
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Toxoplasma Infection ◽  
Urban Zone ◽  
Lack Of Control ◽  
Iga Antibodies

Cats are the definitive hosts of Toxoplasma gondii. Infected cats excrete oocysts in their feces, infecting humans and other animals. The objective of the present study was to determine the presence of anti-Toxoplasma antibodies in cat owners and their pets, and determine if there was a relationship between Toxoplasma infection and humans who live with infected cats. IgG anti-Toxoplasma antibodies in sera of 59 cat owners were determined by enzyme-linked immunosorbent assay (ELISA), in 24 sera from their cats, IgG, IgM, and IgA antibodies were found using Burney's ELISA. Thirty-eight (64%) of 59 cat owners were positive to IgG anti-Toxoplasma. Seropositivity for cats was 70.8% IgG, 8.3% IgM, and 62.5% IgA. Cohabitation with cats infected by T. gondii, feeding with leftovers or raw viscera, and lack of control over how their feces were handled are risk factors conducive for humans to become infected by T. gondii.

scholarly journals Epidemiological survey on gastro-intestinal and blood-borne helminths of dogs in north-east Gabon : research communication

2008 ◽  
Vol 75 (4) ◽  
Author(s):  
B. Davoust ◽  
T. Normand ◽  
O. Bourry ◽  
H. Dang ◽  
E. Leroy ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Epidemiological Survey ◽  
Enzyme Linked Immunosorbent Assay ◽  
Helminth Parasites ◽  
Intestinal Helminths ◽  
Spirocerca Lupi ◽  
Faecal Examination ◽  
Blood Samples ◽  
North East ◽  
Trichuris Vulpis

A survey of helminth parasites was carried out on 198 dogs living in almost complete liberty in villages in the northeast of Gabon. Faeces and blood samples were collected and analysed. Dirofilariaimmitis antigen was detected in 13.6 % of dogs using the SNAP 3Dx® test, a commercially available enzyme-linked immunosorbent assay (ELISA). Faecal examination revealed that 91.4 % of dogs were infected by intestinal helminths. Ascarids were found in 58.5 % of the samples. Trichuris vulpis was observed in 49.5 % of cases, and Uncinaria spp. and Ancylostoma spp. in 34.8 %, Spirocerca lupi in 25.3 % and Capillaria spp. in 10.6 %. Cestode embryophores were found in 8.6 % of the samples.

Measles antibodies and response to vaccination in children aged less than 14 months: implications for age of vaccination

2011 ◽  
Vol 140 (9) ◽  
pp. 1599-1606 ◽  
Author(s):  
E. BORRÀS ◽  
L. URBIZTONDO ◽  
J. COSTA ◽  
J. BATALLA ◽  
N. TORNER ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Maternal Antibodies ◽  
Passive Immunity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Blood Samples ◽  
Measles Outbreak ◽  
Measles Antibodies ◽  
Measles Vaccination

SUMMARYPassive immunity against measles decreases during the first months of life. The objective of this study was to determine titres of measles antibodies in children aged 9–14 months and their mothers before vaccination, and the children's response to vaccination. Blood samples were collected by capillary puncture before and 28 days after vaccination. Samples were obtained between February and June 2007 during an ongoing measles outbreak. Titres of specific measles IgG antibodies were determined by enzyme-linked immunosorbent assay. Seroconversion was defined as the presence of antibodies after vaccination in subjects without antibodies before vaccination. Maternal antibodies were present in 37·7% of all 69 children included and in 45·1% of children aged 9 months. Of the 51 children in whom a second sample was obtained, 31 (60·8%) were seronegative before vaccination and 61·3% seroconverted. Interference of maternal antibodies was 30%. Advancing the first dose of measles vaccination from 15 to 12 months is a correct strategy, given the increase in the time of susceptibility of infants to measles.

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