Measles antibodies and response to vaccination in children aged less than 14 months: implications for age of vaccination

2011 ◽  
Vol 140 (9) ◽  
pp. 1599-1606 ◽  
Author(s):  
E. BORRÀS ◽  
L. URBIZTONDO ◽  
J. COSTA ◽  
J. BATALLA ◽  
N. TORNER ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Maternal Antibodies ◽  
Passive Immunity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Blood Samples ◽  
Measles Outbreak ◽  
Measles Antibodies ◽  
Measles Vaccination

SUMMARYPassive immunity against measles decreases during the first months of life. The objective of this study was to determine titres of measles antibodies in children aged 9–14 months and their mothers before vaccination, and the children's response to vaccination. Blood samples were collected by capillary puncture before and 28 days after vaccination. Samples were obtained between February and June 2007 during an ongoing measles outbreak. Titres of specific measles IgG antibodies were determined by enzyme-linked immunosorbent assay. Seroconversion was defined as the presence of antibodies after vaccination in subjects without antibodies before vaccination. Maternal antibodies were present in 37·7% of all 69 children included and in 45·1% of children aged 9 months. Of the 51 children in whom a second sample was obtained, 31 (60·8%) were seronegative before vaccination and 61·3% seroconverted. Interference of maternal antibodies was 30%. Advancing the first dose of measles vaccination from 15 to 12 months is a correct strategy, given the increase in the time of susceptibility of infants to measles.

scholarly journals Characteristics of measles immunity in students of the pediatric faculty of a medical university

2020 ◽  
Vol 12 (4) ◽  
pp. 58-64
Author(s):  
D. O. Ivanov ◽  
V. N. Timchenko ◽  
E. B. Pavlova ◽  
E. V. Barakina ◽  
T. M. Chernova ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Measles Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Measles Vaccine ◽  
Specific Antibodies ◽  
Measles Antibodies ◽  
Measles Vaccination ◽  
Instructions For Use ◽  
Medical University ◽  
The Relationship

Objective: to evaluate the intensity of measles immunity in students of I–VI courses of the pediatric faculty of St. Petersburg State Medical University.Materials and methods: titers of antibodies to measles virus were determined in 986 students of I–VI courses in May –June 2019. The level of measles antibodies was determined by enzyme-linked immunosorbent assay.Results: positive (protective) titres of measles antibodies were detected in 555 people. (56.4%), the absence of measles immunity was found in 431 people. (43.6%), including 57 people. (5.8%) specific antibodies were not detected. The relationship between the level of collective immunity and the duration of the period after the last vaccination against measles was revealed.Conclusions: in the majority of examined students (96.2%), protective titers of measles antibodies remained for 10 years after the last vaccination. Subsequently, a significant decrease in the level of measles antibodies was noted. All students who do not have a protective IgG titer are shown measles vaccination with live measles vaccine in accordance with the instructions for use. 

scholarly journals Performance Characteristics of an Enzyme-Linked Immunosorbent Assay for Determining Salivary Immunoglobulin G Response toHelicobacter pylori

1999 ◽  
Vol 37 (2) ◽  
pp. 430-432 ◽  
Author(s):  
R. De Pascalis ◽  
M. Del Pezzo ◽  
G. Nardone ◽  
G. Budillon ◽  
A. Lavitola
Keyword(s):  
Immune Response ◽  
Helicobacter Pylori ◽  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Sample Collection ◽  
Blood Samples ◽  
H Pylori

We evaluated the salivary immunoglobulin G (IgG) immune response toHelicobacter pylori in 70 subjects by enzyme-linked immunosorbent assay (ELISA). Subjects with a positive H. pylori culture showed significantly higher titers of antibodies than subjects with no detectable H. pylori: the overall sensitivity and specificity of the test were 84 and 90%, respectively. The detection of salivary anti-H. pyloriIgG antibodies may be considered as an alternative to serum IgG detection for ease of sample collection or when blood samples are not available in screening of patients with dyspepsia.

scholarly journals Development and Validation of 13-plex Luminex-Based Assay for Measuring Human Serum Antibodies to Streptococcus pneumoniae Capsular Polysaccharides

mSphere ◽  
2018 ◽  
Vol 3 (4) ◽  
pp. e00128-18 ◽  
Author(s):  
Danka Pavliakova ◽  
Peter C. Giardina ◽  
Soraya Moghazeh ◽  
Shite Sebastian ◽  
Maya Koster ◽  
...  
Keyword(s):  
Human Serum ◽  
Lower Limit ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Correlate Of Protection ◽  
Immune Correlate ◽  
Relative Standard ◽  
Limit Of Quantitation

ABSTRACT A Luminex-based direct immunoassay (dLIA) platform has been developed to replace the standardized pneumococcal enzyme-linked immunosorbent assay platform. The multiplex dLIA simultaneously measures the concentration of serum immunoglobulin G (IgG) antibodies specific for pneumococcal capsular polysaccharide (PnPS) serotypes 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, and 23F. The assay uses poly-l-lysine (PLL)-conjugated PnPS, chemically coupled to spectrally distinct Luminex microspheres. Assay validation experiments were performed using residual human serum samples obtained from 13-valent pneumococcal conjugate vaccine (13vPnC) clinical studies. Assay results are expressed as IgG antibody concentrations in micrograms per milliliter using the international reference serum, 007sp. The lower limit of quantitation (LLOQ) for all serotypes covered in the 13-plex dLIA fell within the range of 0.002 to 0.038 µg/ml serum IgG. The difference between the lower limit and upper limit of the assay range was >500-fold for all serotypes, and assay variability was <20% relative standard deviation (RSD) for all serotypes. IgG antibody measurements were shown to be serotype-specific (some cross-reactivity was observed only between the structurally related serotypes 6A and 6B as well as 19A and 19F), and no interference was observed between the serotypes when the assay was performed in the 13-plex format compared to the singleplex assays. The 13-plex dLIA platform developed by Pfizer Inc. generates up to 143 test results in a single 96-well plate and is a suitable replacement of the enzyme-linked immunosorbent assay (ELISA) platform for evaluating vaccine clinical trials. IMPORTANCE The pneumococcal enzyme-linked immunosorbent assay (ELISA) measures IgG antibodies in human serum, and it is an important assay that supports licensure of pneumococcal vaccines. The immune correlate of protection, 0.35 µg/ml of IgG antibodies, was determined by the ELISA method. Pfizer has developed a new Luminex-based assay platform to replace the ELISA. These papers describe the important work of (i) validating the Luminex-based assay and (ii) bridging the immune correlate of protection (0.35 µg/ml IgG) to equivalent values reported by the Luminex platform.

scholarly journals Epidemiological survey on gastro-intestinal and blood-borne helminths of dogs in north-east Gabon : research communication

2008 ◽  
Vol 75 (4) ◽  
Author(s):  
B. Davoust ◽  
T. Normand ◽  
O. Bourry ◽  
H. Dang ◽  
E. Leroy ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Epidemiological Survey ◽  
Enzyme Linked Immunosorbent Assay ◽  
Helminth Parasites ◽  
Intestinal Helminths ◽  
Spirocerca Lupi ◽  
Faecal Examination ◽  
Blood Samples ◽  
North East ◽  
Trichuris Vulpis

A survey of helminth parasites was carried out on 198 dogs living in almost complete liberty in villages in the northeast of Gabon. Faeces and blood samples were collected and analysed. Dirofilariaimmitis antigen was detected in 13.6 % of dogs using the SNAP 3Dx® test, a commercially available enzyme-linked immunosorbent assay (ELISA). Faecal examination revealed that 91.4 % of dogs were infected by intestinal helminths. Ascarids were found in 58.5 % of the samples. Trichuris vulpis was observed in 49.5 % of cases, and Uncinaria spp. and Ancylostoma spp. in 34.8 %, Spirocerca lupi in 25.3 % and Capillaria spp. in 10.6 %. Cestode embryophores were found in 8.6 % of the samples.

scholarly journals Επιπολασμός λοίμωξης από ελικοβακτηρίδιο του πυλωρού σε ασθενείς με χρόνια αποφρακτική πνευμονοπάθεια

2005 ◽  
Author(s):  
Αναστάσιος Ρούσσος
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies

Ασθενείς με πεπτικό έλκος εμφανίζουν αυξημένο επιπολασμό Χρόνιας Αποφρακτικής Πνευμονοπάθειας (ΧΑΠ) . Το κάπνισμα το οποίο αποτελεί κοινό προδιαθετικό παράγοντα και για τα δύο αυτά νοσήματα έχει ενοχοποιηθεί για τη συσχέτιση αυτή Όμως, πρόσφατες μελέτες έδειξαν ότι η ασθενείς με χρόνια βρογχίτιδα, ίσως, παρουσιάζουν αυξημένο επιπολασμό Η. pylori λοίμωξης Είναι γνωστό ότι σε ορισμένους ασθενείς η λοίμωξη από Η. pylori ενεργοποιεί την απελευθέρωση μίας σειράς προφλεγμονωδών κυτοκινών. Υπεύθυνα για την παραγωγή αυτών των κυτοκινών θεωρούνται τα ιδιαίτερα λοιμογόνα στελέχη Η. pylori τα οποία παράγουν την πρωτεΐνη CagA. Οι προφλεγμονώδεις αυτές κυτοκίνες εμπλέκονται και στην παθογένεια της ΧΑΠ, πιθανότατα προάγοντας τη μη ειδική φλεγμονή του βρογχικού δέντρου. Σκοπός της παρούσας μελέτης ήταν η διερεύνηση του επιπολασμού της Η. pylori λοίμωξης και ιδιαίτερα των CagA θετικών λοιμογόνων στελεχών σε ασθενείς με ΧΑΠ. Επίσης, επιχειρήθηκε η συσχέτιση της οροθετικότητας για το Η. pylori και για τα CagA θετικά στελέχη του με κλινικοεργαστηριακές παραμέτρους της ΧΑΠ (σπιρομετρικός έλεγχος και βαρύτητα της νόσου) Συνολικά μελετήθηκαν 126 ασθενείς με ΧΑΠ (88 άντρες and 38 γυναίκες με ηλικία 61.3 ± 8.1 έτη) και 126 μάρτυρες, προτυποποιημένοι κατά φύλο ηλικία και κοινωνικοοικονιμικό επίπεδο. Όλα τα άτομα τα οποία συμπεριελήφθησαν στη μελέτη (ασθενείς και μάρτυρες) υποβλήθηκαν σε ενζυμική ανοσοπροσροφητική μέθοδο προσδιορισμού [enzyme-linked immunosorbent assay (elisa)] των IgG αντισωμάτων για το Η. pylori και την πρωτεΐνη CagA. Επίσης, οι ασθενείς με ΧΑΠ υποβλήθηκαν σε σπιρομετρικό έλεγχο (FEV1, FEV1/FVC) και σε συνακόλουθη σταδιοποίηση της βαρύτητας της νόσου Ο εττιπολασμός της FI pylori λοίμωξης ήταν υψηλότερος στους ασθενείς με ΧΑΠ συγκριτικά με τους μάρτυρες. [77.8% έναντι 54.7% αντίστοιχα (ρ<0.001)]. Ο επιπολασμός της λοίμωξης από CagA (+) Η pylori στελέχη ήταν υψηλότερος στους ασθενείς με ΧΑΠ συγκριτικά με τους μάρτυρες [53.9% έναντι 29.3% αντίστοιχα (ρΟ.ΟΟΙ)]. Επίσης, οι ασθενείς με ΧΑΠ είχαν σημαντικά υψηλότερη μέση συγκέντρωση τόσο anti-FI. pylori IgG (118.3±24.4 vs 61.9±12.9 U/ML, ρ<0.001) όσο και anti-CagA IgG antibodies (33.8±3.4 vs 19.0±1.5 U/ML, ρ<0.001). Οι σπιρομετρικές παράμετροι δεν διέψεραν σημαντικά μεταξύ των Fi pylori (+) και Η pylori (-) ασθενών με ΧΑΠ (FEV1:61.5±18.9%, FEV1/FVC 63.0± 4.9%, έναντι FEV1:63.5±17.9%, FEV1/FVC: 63.9± 4.6%, αντίστοιχα, ρ>0.05). Παρομοίως, οι σπιρομετρικές παράμετροι δεν διέφεραν σημαντικά μεταξύ των CagA(+) και CagA (-) ασθενών με ΧΑΠ (FEV1: 59.1 ±9.7%, FEV1/FVC 62.4± 5.1%, έναντι FEV1: 65.4±6.3%, FEV1/FVC: 64.3± 4.3%, αντίστοιχα, ρ>0.05) Τέλος, δεν αναδείχθηκε σημαντική διαφορά μεταξύ των ποσοστών αντί- FI pylori IgG θετικότητας και αντι-CagA IgG θετικότητας στα διάφορα στάδια ΧΑΠ. Με δεδομένο τον υψηλό επιπολασμό της ελικοβακτηριδιακής λοίμωξης σε ασθενείς με ΧΑΠ και ιδιαίτερα των λοιμογόνων Caga (+) στελεχών τα οποία ενοχοποιούνται για την παραγωγή προφλεγμονωδών κυτοκινών (όμοιες με αυτές που εμπλέκονται στην παθογένεια της ΧΑΠ) θεωρείται πιθανός ο παθογενετικός ρόλος του ελικοβακτηριδίου στη ΧΑΠ. Αντίθετα, περιορισμένος φαίνεται να είναι ο ρόλος τον οποίο διαδραματίζει το ελικοβακτηρίδιο στην εξέλιξη της νόσου, καθώς δε διαπιστώθηκε συσχέτιση της Η pylori οροθετικότητας και της CagA οροθετικότητας με τη βαρύτητα της ΧΑΠ Σίγουρα στο μέλλον απαιτούνται μελέτες σε μεγαλύτερο αριθμό ασθενών οι οποίες να ελέγχουν την ορθότητα των ευρημάτων μας.

scholarly journals An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses

2004 ◽  
Vol 34 (5) ◽  
pp. 1525-1529 ◽  
Author(s):  
Cristiane Divan Baldani ◽  
Rosangela Zacarias Machado ◽  
Paulo de Tarso Landgraf Botteon ◽  
Felipe Santoro Takakura ◽  
Carlos Luiz Massard
Keyword(s):  
Immunosorbent Assay ◽  
Serological Test ◽  
Epidemiological Studies ◽  
Sao Paulo ◽  
Enzyme Linked Immunosorbent Assay ◽  
São Paulo ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Babesia Equi ◽  
Specificity And Sensitivity

A crude antigenic preparation of Babesia equi was used to develop and establish the suitability of an enzyme-linked immunosorbent assay (ELISA) for the detection of parasite carriers. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkboard titrations. The specificity and sensitivity of the ELISA were 100 %. A total of 90 serum samples were taken from horses from the Northeast region of São Paulo State and examined for diagnosis of equine B. equi infection by ELISA. Approximately 75% (n=67) of all the horses tested were found serologically positive for B. equi. These results suggest that the ELISA described may prove to be an appropriate serological test for epidemiological studies on B. equi infections in the field and that equine piroplasmosis is a cause for serious concern in the State of São Paulo, Brazil.

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