A bioactive compound isolated from Duku (Lansium domesticum Corr) fruit peels exhibits cytotoxicity against T47D cell line

Background: Breast cancer is a major health problem for women globally. Many attempts have been promoted to cure cancer by finding new anticancer medicines from natural resources. Despite the richness of biodiversity discovered, there are some natural resources that remain unexplored. Fruit peels of Duku (Lansium domesticum Corr.) are rich with compounds that may have the potential to be developed as anticancer drugs. This study aimed to isolate cytotoxic compounds from the fruit peels of L. domesticum and assess their cytotoxic nature against T47D cells. Methods: Powdered peels were macerated with ethyl acetate and the filtrate was evaporated to give EtOAc extract A. Dried extract A was triturated with n-hexane to give n-hexane soluble fraction B and insoluble fraction C. The cytotoxic nature of these three samples were assessed using MTT assay using T47D cells and doxorubicin as a control. Results: Fraction C that showed the smallest IC50 (25.56 ± 0.64μg/mL) value compared to extract A and fraction B. Fraction C was further fractionated by vacuum liquid chromatography to give 6 subfractions. Subfraction 2 showed a single compound based on thin layer chromatography, and this compound was identified as Lamesticumin A on the basis of its spectroscopic data. Lamesticumin A demonstrated cytotoxic activity against T47D cell lines with an IC50 value of 15.68 ± 0.30µg/mL. Conclusions: Further research is needed to investigate the potential of the natural compound Lamesticumin A derived from L. domesticum fruit peel as an anticancer therapy.

Download Full-text

A Sesquiterpene Aldehyde Isolated From Ethyl Acetate Extract of Lansium Domesticum Fruit Peel

INDONESIAN JOURNAL OF PHARMACY ◽

10.22146/ijp.1884 ◽

2021 ◽

pp. 394-398

Author(s):

Khusnul Fadhilah ◽

Subagus Wahyuono ◽

Puji Astuti

Keyword(s):

Ethyl Acetate ◽

Cell Lines ◽

Mobile Phase ◽

Spectroscopic Data ◽

Biological Activities ◽

Preparative Thin Layer Chromatography ◽

Insoluble Fraction ◽

Fruit Peel ◽

Etoac Extract ◽

Lansium Domesticum

Lansium domesticum (fam. Meliaceae) contains various compounds with various biological activities. Based on the previous research, extracts from several parts of the plant have biological activity. This study aimed to isolate a compound from the fruitpeel of L. domesticum and evaluate cytotoxic activity against T47D, WiDr and HepG2 cell lines. Powdered peels were macerated with ethyl acetate and the filtrate was evaporated to give EtOAc extract. Dried extract was triturated with n-hexane to give n-hexane soluble fraction (A) and insoluble fraction (B). The fraction B was separated using vacuum column chromatography (VLC) with mobile phase n-hexane: ethyl acetate and given 5 fractions. Fractions B3-B5 were combined and separated using VLC with n-hexane and ethyl acetate as mobile phase. This VLC separation gave 18 subractions, subfractions 6-9 with the similar TLC profile were combined. This subfraction was separated further using preparative thin layer chromatography to give compound 1. The Isolated compound (1) appeared as liquid. The chemical structure of 1 was identified acoording to spectroscopic data and comparison with literature. Cytotoxic bioassay was performed on T-47D, WiDr and Hep G2 cell lines in a series of concentrations at 50, 40, 30, 20, 10 and 5µg/mL, with Doxorubicine used as positive control. According to spectroscopic data, compound 1 was identified as 2-ethyl,3-(1’-hydroxy-2’-menthene) propenal, and demonstrate the strongest cytotoxicity against T-47D cell lines (IC50=39.18+1.54 µg/mL).

Download Full-text

Adenosine A1 Receptor Antagonist Up-regulates Casp3 and Stimulates Apoptosis Rate in Breast Cancer Cell Line T47D

International Electronic Journal of Medicine ◽

10.34172/iejm.2020.03 ◽

2020 ◽

Vol 9 (1) ◽

pp. 14-20

Author(s):

Mohammad Zamani Rarani ◽

Fahimeh Zamani Rarani ◽

Ali Valiani ◽

Zeinolabedin Shrifian Dastjerdi ◽

Elias Kargar Abargouei ◽

...

Keyword(s):

Cell Line ◽

Cancer Cell Line ◽

P53 Gene ◽

T47d Cell ◽

Adenosine A1 Receptor ◽

T47d Cells ◽

Apoptosis Rate ◽

Adenosine A1 ◽

T47d Cell Line ◽

A1 Receptor

Background: Adenosine receptor family, especially A1 type is-overexpressed in breast-derived tumor cells and the P53 gene is mutant in some of these cells while the casps gene is of wild type as well. The aim of this study was to evaluate the effect of the A1 receptor function on cell programmed death or proliferation, as well as the relationship between this receptor stimulation/inhibition and caspase 3 (casp3) expression in T47D cell line that has a mutant and non-functional P53 gene. Materials and Methods: The expression of casps3 was measured by real-time polymerase chain reaction and then flow cytometery and MTT assay were used to assess the apoptotic and proliferation cell rate after the treatment of T47D cells with specific agonist N6-cyclopentyladenosine (CPA) and antagonist 1,3-dipropyl-8-cyclopentylxanthine (DPCPX) of this receptor 24, 48, and 72 hours after treatment. Result: Our results indicated that DPCPX significantly induces apoptosis in T47D cells and the rate of survival cell after the reduction of this treatment, especially 72 hours after treatment. Finally, the expression of casp3 was up-regulated by DPCPX treatment, especially in 72 hours while CPA treatment had opposite results (P>0.05). Conclusion: In general, DPCPX could up-regulate casp3 gene expression and subsequently increase the apoptosis rate in T47D cells with casp3 expression without the P53 gene interference. Therefore, adenosine A1 receptor antagonists may be introduced as anti-cancer agents.

Download Full-text

Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d220918 ◽

2021 ◽

Vol 22 (9) ◽

Author(s):

Khusnul Fadhilah ◽

Subagus Wahyuono ◽

Puji Astuti

Keyword(s):

Cytotoxic Activity ◽

Hepg2 Cell ◽

Cell Lines ◽

Biological Activities ◽

Fruit Peel ◽

Tropical Fruit ◽

Etoac Extract ◽

Isolated Compound ◽

Hepg2 Cell Lines ◽

Lansium Domesticum

Abstract. Fadhilah K, Wahyuono S, Astuti P. 2021. Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines. Biodiversitas 22: 3743-3748. Lansium domesticum (Fam. Meliaceae), a tropical fruit (local name, Duku), has been reported to have various biological activities. At the moment we emphasize on searching compounds to have cytotoxic activity from the peel of L. domesticum. The study was initiated by extraction with EtOAc followed by fractionation that was monitored by thin-layer chromatography (TLC) and cytotoxicity against T-47D and HepG2 cells. The EtOAc extract of the sample was triturated with n-hexane to give n-hexane soluble (A) n-hexane insoluble fractions (B). The B fraction was fractionated by vacuum column chromatography using gradient solvent composition of n-hexane: acetone to give 18 fractions. According to TLC similarity pictures, fractions were combined to give 6 fractions (I-VI). Isolation was performed using preparative TLC, and the cytotoxic assay was performed using MTT method. The isolated compound was identified as a sesquiterpene having one aldehyde functional group, based on spectroscopic data.The isolated compound displayed cytotoxic activity on T-47D (IC50, 48.58 + 0.96 µg/mL) and HepG2 (IC50, 127.45 + 25.76 µg/mL).

Download Full-text

New derivatives of a natural nordentatin

Open Chemistry ◽

10.1515/chem-2020-0149 ◽

2020 ◽

Vol 18 (1) ◽

pp. 890-897 ◽

Cited By ~ 1

Author(s):

Tin Myo Thant ◽

Nanik Siti Aminah ◽

Alfinda Novi Kristanti ◽

Rico Ramadhan ◽

Hnin Thanda Aung ◽

...

Keyword(s):

Cell Line ◽

Parent Compound ◽

T47d Cell ◽

Positive Control ◽

Inhibition Assay ◽

Anticancer Activities ◽

Methanol Fraction ◽

T47d Cell Line ◽

Ft Ir ◽

Derivatives Of

AbstractNew derivatives were obtained from natural nordentatin (1) previously isolated from the methanol fraction of Clausena excavata by an acylation method. Herein, we report ten new pyranocoumarin derivatives 1a–1j. Their structures were elucidated based on UV-vis, FT-IR, NMR, and DART-MS data. The α-glucosidase inhibition and anticancer activities of nordentatin (1) and its derivatives were also evaluated. The α-glucosidase inhibition assay exhibited that the derivatives 1b, 1d, 1e, 1f, 1h, 1i, and 1j possess higher inhibitory activity for α-glucosidase with IC50 values of 1.54, 9.05, 4.87, 20.25, 12.34, 5.67, and 2.43 mM, whereas acarbose was used as the positive control, IC50 = 7.57 mM. All derivatives exhibited a weak cytotoxicity against a cervical cancer (HeLa) cell line with the IC50 between 0.25 and 1.25 mM. They also showed moderate to low growth inhibition of a breast cancer (T47D) cell line with IC50 values between 0.043 and 1.5 mM, but their activity was lower than that of the parent compound, nordentatin (1) (IC50 = 0.041 mM).

Download Full-text

The radical scavenging activity of 2-2’ diphenyl -1- picrylhydrazil (DPPH) on the methanol extracts and Ethyl acetate fractions of red dragon fruit peel (Hylocereus polyrhizus (F.A.C.Weber) Britton dan Rose)

International Journal of Phytomedicine ◽

10.5138/09750185.1945 ◽

2017 ◽

Vol 9 (1) ◽

pp. 79

Author(s):

Sri Wahdaningsih ◽

Subagus Wahyuono ◽

Sugeng Riyanto ◽

Retno Murwanti

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Soluble Fraction ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Insoluble Fraction ◽

Fruit Peel ◽

Activity Test ◽

Dragon Fruit

Red dragon fruit (H. Polyrhizus) is one of the the plants that has a great potential as natural antioxidant. This study tested the activity of radical scavenging of 2-2' diphenyl -1- pikril hidrazil (DPPH) in the methanol extract, as well as in the soluble and insoluble fractions of ethyl acetate of red dragon fruit peel. This research is carried out through various stages, such as: extraction and fractionation to obtain both insoluble fraction and soluble fractions of ethyl acetate. Antioxidant activity test is conducted by the method of thin layer chromatography and spectrophotometry. Antioxidant activity test, IC50 values of methanol extract, ethyl acetate soluble fraction, and insoluble fraction of ethyl acetate had been obtained consecutively as much as 241.19 µg /mL, 8.34 µg/mL, 46.84 µg/mL. The soluble fraction of ethyl acetate had greater antioxidant activity compared to the methanol extract and the insoluble fractions of ethyl acetate.

Download Full-text