Life course programming of stress responses in adolescents and young adults in India: Protocol of the Stress Responses in Adolescence and Vulnerability to Adult Non-communicable disease (SRAVANA) Study

Background: Early life nutrition may affect individuals’ susceptibility to adult non-communicable diseases (NCD). Psychological stress is a well-recognised NCD risk factor. Recent evidence suggests that impaired foetal nutrition alters neuro-endocrine pathways, and hypothalamic-pituitary-adrenal axis feedback systems, resulting in abnormal stress responses, and NCD risk. This study aims to examine adolescent cortisol and cardiovascular stress responses in relation to maternal nutrition and contemporaneous NCD risk markers. Methods: The study sample will be drawn from three well-established birth cohorts in India; the Parthenon cohort, Mysore (N=550, age~20y), the SARAS KIDS prenatal intervention cohort, Mumbai (N=300, age~10-12y) and the Pune Rural Intervention in Young Adults/ PRIYA cohort, Pune (N=100, age~22y). We will perform the ‘Trier Social Stress Test (TSST)’, a well-accepted stress-test module which involves participants performing 5-minutes each of public speaking and mental arithmetic tasks in front of unfamiliar ‘judges’ (stressor). Repeated measures of salivary cortisol and autonomic cardiovascular outcomes relative to the stressor will be assessed. Measures of psychological stress, cognitive function, blood pressure, glucose-insulin metabolism and depression will be carried out. Mechanistic studies including DNA methylation in gluco-corticoid receptor (NR3C1) and 11β-HSD2 gene loci and neuroimaging will be carried out in a subsample. Qualitative interviews and focus group discussions in a subsample of the Parthenon cohort will explore the perception of stress and stressors among the youth. We will convert repeated measures into time-weighted averages before analysis. We will carry out multivariable regression analysis to test the associations. We will further refine the analyses using the mixed-model regression and conditional analyses for the association with repeated measures. Ethics and dissemination: This study has been approved by the research ethics committee of CSI Holdsworth Memorial Hospital, Mysore. The findings will be disseminated locally and at international meetings, and reports will be submitted to open access peer reviewed journals.

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The Relationship Between Perceived Control and Hypothalamic–Pituitary–Adrenal Axis Reactivity to the Trier Social Stress Test in Healthy Young Adults

Frontiers in Psychology ◽

10.3389/fpsyg.2021.683914 ◽

2021 ◽

Vol 12 ◽

Author(s):

Qian Liu ◽

Jianhui Wu ◽

Liang Zhang ◽

Xiaofang Sun ◽

Qing Guan ◽

...

Keyword(s):

Young Adults ◽

Stress Response ◽

Hpa Axis ◽

Stress Responses ◽

Perceived Control ◽

Social Stress ◽

Stress Test ◽

Control Level ◽

Trier Social Stress Test ◽

Hypothalamic Pituitary Adrenal

Psychological factors can modulate the hypothalamic–pituitary–adrenal (HPA) axis activity toward stressors. Animal studies demonstrated that uncontrollability was one critical factor associated with HPA axis stress response, but the results in human studies were inconsistent. The current study adopted a standardized laboratory stress induction procedure, the Trier Social Stress Test (the TSST), as the stressor to regulate the objective controllability level, and young adult participants were asked to rate their subjectively perceived control level toward the stressor and measured their cortisol stress responses (N=54; 19 females and 35 males) to address this concern. Results showed that participants’ perceived control on the TSST was related to the cortisol stress response. In other words, under the stress of a certain objective controllability level, the lower the subjectively perceived control level, the greater the HPA axis response. This finding suggested that, in addition to objective controllability, subjectively perceived control is a psychological factor that regulates activation of the HPA axis in young adults.

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The effects of acute psychosocial stress on executive functioning in young and older adults

10.32920/ryerson.14647257.v1 ◽

2021 ◽

Author(s):

Leen Nasser

Keyword(s):

Older Adults ◽

Young Adults ◽

Executive Functioning ◽

Stress Responses ◽

Social Stress ◽

Acute Stress ◽

Stress Test ◽

Trier Social Stress Test ◽

Age Related ◽

Effects Of Stress

It has been evidenced that, with aging, older adults exhibit increased behavioral and physiological responses to stress. Older adults also often experience declines in executive functioning performance. The acute psychological stress induced through the Trier Social Stress Test (TSST) has been evidenced to negatively impact executive functioning in young adults. This relationship, however, has yet to be examined in older adults. In the current thesis, two experiments were conducted to investigate the effects of stress on executive functioning (Experiment 1), as well as age related differences in stress responsivity and in the effect of stress on executive functioning (Experiment 2). In Experiment 1, acute stress exhibited a negative effect on executive functioning. In Experiment 2, there were no age differences in stress responses, and a positive effect of acute stress on executive functioning in young adults only. The contradictory findings encourage further research on the effects of stress on executive functioning, and how they may differ between young and older adults.

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Abstract 12683: Genome-wide Mrna Expression Analysis of Acute Psychological Stress Responses

Circulation ◽

10.1161/circ.142.suppl_3.12683 ◽

2020 ◽

Vol 142 (Suppl_3) ◽

Author(s):

Jeongok G Logan ◽

Mihyun Jeong

Keyword(s):

Psychological Stress ◽

Stress Responses ◽

Stress Test ◽

Trier Social Stress Test ◽

Control Group ◽

Genome Wide ◽

Before And After ◽

Acute Psychological Stress ◽

Distress Scale ◽

Experimental Group

Introduction: Previous studies have examined effects of acute psychological stress in humans on selected panels of genes. This exploratory study aimed to investigate genome-wide transcriptional activity changes in responses to acute psychological stress. Methods: The sample included 40 healthy women who participated in a previous study (22 who had a stress induced experience (experimental group) and 18 who did not (control group)). The psychological stress was induced by the Trier Social Stress Test (TSST). Psychological stress levels (measured by the Subjective Units of Distress Scale (SUDS) and the state anxiety subscale of the Spielberger State-Trait Anxiety Inventory) and hemodynamic changes (measured by blood pressure (BP) and heart rate (HR)) were assessed before and after the TSST. The peripheral blood samples obtained before and after the TSST were processed for mRNA-sequencing. Results: The mean age of the participants was 31 years (SD 11.6). The psychological and hemodynamic stress parameters indicated that the TSST successfully induced moderate but statistically significant levels of acute psychological stress in the participants of the experimental group. Compared with the participants in the control group, six genes ( BCL2L14, FAM3B, HCG26, HLA-F-AS1, LOC101928710 , and SLC22A16 ) were up-regulated and 9 genes ( ATP2C2, CA1, CRYBG3, FBXO9, HBD, SLC39A9, SNCA, STRADB , and TRMT12 ) were down-regulated among those who experienced stress induction. The IPA analysis identified one network of cell-to-cell signaling and interaction, cellular function and maintenance, and hematological system development and function. Thirteen genes out of 15 were found in this network. Conclusions: The identified genes including FAM3B, BCL2L14, SLC22A16 , and SNCA , have been proposed as the therapeutic targets of diabetes-related CVDs, certain cancers, and Parkinson’s disease, but have not previously been reported to be associated with psychological stress. Future studies are suggested to examine the pathological mechanisms by which the identified genes may mediate the association between psychological stress and adverse health outcome.

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The effects of acute psychosocial stress on executive functioning in young and older adults

10.32920/ryerson.14647257 ◽

2021 ◽

Author(s):

Leen Nasser

Keyword(s):

Older Adults ◽

Young Adults ◽

Executive Functioning ◽

Stress Responses ◽

Social Stress ◽

Acute Stress ◽

Stress Test ◽

Trier Social Stress Test ◽

Age Related ◽

Effects Of Stress

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Maternal B12, Folate and Homocysteine Concentrations and Offspring Cortisol and Cardiovascular Responses to Stress

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/clinem/dgz114 ◽

2020 ◽

Vol 105 (7) ◽

pp. e2591-e2599

Author(s):

Ghattu V Krishnaveni ◽

Sargoor R Veena ◽

Matt Johnson ◽

Kalyanaraman Kumaran ◽

Alexander Jones ◽

...

Keyword(s):

Heart Rate ◽

Vitamin B12 ◽

Stress Responses ◽

Total Peripheral Resistance ◽

Mental Arithmetic ◽

Stress Test ◽

Peripheral Resistance ◽

Cardiovascular Responses ◽

Trier Social Stress Test ◽

Plasma Vitamin

Abstract Context Imbalances in maternal 1-carbon nutrients (vitamin B12, folate) have been shown to be associated with higher offspring cardiometabolic risk markers in India. Objective We examined the hypothesis that low plasma vitamin B12 (B12) and high folate and homocysteine concentrations in the mother are associated with higher hypothalamic–pituitary–adrenal axis (cortisol) and cardiovascular responses during the Trier Social Stress Test for Children (TSST-C) in an Indian birth cohort. Methods Adolescents (n = 264; mean age: 13.6 years), whose mothers’ plasma B12, folate and total homocysteine concentrations had been measured during pregnancy, completed 5-minutes each of public speaking and mental arithmetic tasks in front of 2 unfamiliar “judges” (TSST-C). Baseline and poststress salivary cortisol concentrations were measured. Heart rate, blood pressure, stroke volume, cardiac output, and total peripheral resistance were measured continuously at baseline, during the TSST-C, and for 10 minutes after the TSST-C using a finger cuff; beat-to-beat values were averaged for these periods, respectively. Results Maternal low B12 status (plasma B12 < 150 pmol/L) was associated with greater cortisol responses to stress in the offspring (P < .001). Higher homocysteine concentrations were associated with greater offspring heart rate response (P < .001). After adjustment for multiple comparisons, there were nonsignificant associations between higher maternal folate concentrations and offspring total peripheral resistance response (P = .01). Conclusion Our findings suggest that maternal 1-carbon nutritional status may have long-term programming implications for offspring neuroendocrine stress responses.

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The ‘Trier Social Stress Test’ – A Tool for Investigating Psychobiological Stress Responses in a Laboratory Setting

Neuropsychobiology ◽

10.1159/000119004 ◽

1993 ◽

Vol 28 (1-2) ◽

pp. 76-81 ◽

Cited By ~ 3021

Author(s):

Clemens Kirschbaum ◽

Karl-Martin Pirke ◽

Dirk H. Hellhammer

Keyword(s):

Stress Responses ◽

Social Stress ◽

Stress Test ◽

Trier Social Stress Test ◽

Laboratory Setting

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Stress system dysfunction revealed by integrating reactivity of stress pathways to psychological stress in lean and overweight/obese men

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00276.2021 ◽

2021 ◽

Author(s):

Sisitha Udara Jayasinghe ◽

Sarah Janet Hall ◽

Susan Jane Torres ◽

Anne Isabella Turner

Keyword(s):

Blood Pressure ◽

Systolic Blood Pressure ◽

Diastolic Blood Pressure ◽

Psychological Stress ◽

Hpa Axis ◽

Social Stress ◽

Blood Pressure Response ◽

Stress Test ◽

Trier Social Stress Test ◽

Stress System

While the patterns of response within the sympatho-adrenal medullary (SAM) system and hypothalamo-pituitary adrenal (HPA) axis are interesting and important in their own accord, the overall response to acute psychological stress involves reactivity of both pathways We tested the hypothesis that consideration of the integrated response of these pathways may reveal dysregulation of the stress systems that is not evident when considering either system alone. Age matched lean and overweight/obese men were subjected to a Trier Social Stress Test and reactivity of the SAM system (salivary alpha amylase, systolic blood pressure, diastolic blood pressure and heart rate) and the HPA axis (salivary cortisol) were measured. Relative reactivity of SAM system and HPA axis was calculated as the ratio between the measures from each pathway. While analysis of reactivity of individual stress pathways showed no evidence of dysfunction in overweight/obese compared with lean men, analysis of HPA/SAM reactivity revealed significantly lower cortisol over systolic blood pressure (CoSBP) and cortisol over diastolic blood pressure (CoDBP) reactivity in overweight/obese compared with lean men. Other measures of HPA/SAM reactivity and all measures of SAM/HPA reactivity were unaltered in overweight/obese compared with lean men. These findings suggest that the cortisol response per unit of blood pressure response is blunted in men with elevated adiposity. Further, these findings support a notion of a coordinated overall approach to activation of the stress pathways with the degree of activation in one pathway being related to the degree of activation of the other.

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Brief cognitive intervention can modulate neuroendocrine stress responses to the Trier Social Stress Test: Buffering effects of a compassionate goal orientation

Psychoneuroendocrinology ◽

10.1016/j.psyneuen.2014.02.016 ◽

2014 ◽

Vol 44 ◽

pp. 60-70 ◽

Cited By ~ 40

Author(s):

James L. Abelson ◽

Thane M. Erickson ◽

Stefanie E. Mayer ◽

Jennifer Crocker ◽

Hedieh Briggs ◽

...

Keyword(s):

Goal Orientation ◽

Stress Responses ◽

Social Stress ◽

Stress Test ◽

Cognitive Intervention ◽

Trier Social Stress Test ◽

Buffering Effects

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Beta-adrenergic Modulation of Cognitive Flexibility during Stress

Journal of Cognitive Neuroscience ◽

10.1162/jocn.2007.19.3.468 ◽

2007 ◽

Vol 19 (3) ◽

pp. 468-478 ◽

Cited By ~ 146

Author(s):

Jessica K. Alexander ◽

Ashleigh Hillier ◽

Ryan M. Smith ◽

Madalina E. Tivarus ◽

David Q. Beversdorf

Keyword(s):

Cognitive Flexibility ◽

Social Stress ◽

Public Speaking ◽

Mental Arithmetic ◽

Stress Test ◽

Locus Ceruleus ◽

Trier Social Stress Test ◽

Beta Adrenergic ◽

Impaired Performance ◽

Remote Associates Test

Stress-induced activation of the locus ceruleus-norepinephrine (LC-NE) system produces significant cognitive and behavioral effects, including enhanced arousal and attention. Improvements in discrimination task performance and memory have been attributed to this stress response. In contrast, for other cognitive functions that require cognitive flexibility, increased activity of the LC-NE system may produce deleterious effects. The aim of the present study was to determine the effect of pharmacological modulation of the LC-NE system on stress-induced impairments in cognitive flexibility performance in healthy individuals. Cognitive performance, plus psychological and physiological parameters for 16 adults without any history of anxiety disorders, was assessed during four test sessions: stress and no-stress, with each condition tested after administration of propranolol and placebo. The Trier Social Stress Test, a public-speaking and mental arithmetic stressor, was presented to participants for the stress sessions, whereas a similar, but nonstressful, control task (reading, counting) was utilized for the no-stress sessions. Tests of cognitive flexibility included lexical-semantic and associative problem-solving tasks (anagrams, Compound Remote Associates Test). Visuo-spatial memory and motor processing speed tests served as control tasks. Results indicate that (1) stress impaired performance on cognitive flexibility tasks, but not control tasks; (2) compared to placebo, cognitive flexibility improved during stress with propranolol. Therefore, psychological stress, such as public speaking, negatively impacts performance on tasks requiring cognitive flexibility in normal individuals, and this effect is reversed by beta-adrenergic antagonism. This may provide support for the hypothesis that stress-related impairments in cognitive flexibility are related to the noradrenergic system.

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