scholarly journals Rapid spread of double East- and West-African kdr mutations in wild Anopheles coluzzi from Côte d’Ivoire

2019 ◽  
Vol 4 ◽  
pp. 31 ◽  
Author(s):  
Chouaïbou Seïdou Mouhamadou ◽  
Prisca Bédjou N’Dri ◽  
Behi Kouadio Fodjo ◽  
Christabelle Gba Sadia ◽  
France-Paraudie Kouadio Affoue ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Vector Control ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
West African ◽  
Malaria Vectors ◽  
Cote D’Ivoire ◽  
Côte D'ivoire ◽  
Rapid Spread ◽  
East And West

Malaria morbidity and mortality rates in Sub-Saharan Africa are increasing. The scale-up of long-lasting insecticidal nets and indoor residual spraying have been the major contributors to the decrease of malaria burden. These tools are now threatened by insecticide resistance in malaria vectors, which is spreading dramatically. After two different real-time polymerase chain reaction molecular characterizations carried out on 70 mosquitoes sampled in the locality of Elibou in southern Côte d’Ivoire, results revealed that 9 mosquitoes from Anopheles coluzzi harbored the double East- and West-African knockdown resistance mutations. In the previous year, only 1 mosquito out of 150 sampled from 10 regions of the country had the same genotype. These results show the rapid spread of insecticide resistance in malaria vectors and highlight the urgent need to diversify the methods of vector control in order to avoid the failure of insecticide-based vector control tools which may favor malaria fatalities.

scholarly journals Efficacy of Interceptor® G2, a new long-lasting insecticidal net against wild pyrethroid-resistant Anopheles gambiae s.s. from Côte d’Ivoire: a semi-field trial

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 42 ◽  
Author(s):  
Soromane Camara ◽  
Ludovic Phamien Ahoua Alou ◽  
Alphonsine Amanan Koffi ◽  
Yao Cyntia Muriel Clegban ◽  
Jean-Paul Kabran ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Vector Control ◽  
Anopheles Gambiae ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Blood Feeding ◽  
Phase Iii ◽  
Anopheles Gambiae S.S ◽  
Cote D’Ivoire ◽  
Côte D'ivoire

Background: The widespread insecticide resistance in malaria vector populations is a serious threat to the efficacy of vector control tools. As a result, the World Health Organization (WHO) supports the development of alternative tools that combine several insecticides with the aim of improving vector control and the management of insecticide resistance. In the present study, a long-lasting insecticidal net treated with a mixture of chlorfenapyr and alphacypermethrin was evaluated against wild pyrethroid-resistant Anopheles gambiae s.s in M’bé, Côte d’Ivoire. Centers for Disease Control and Prevention (CDC) bottle tests were carried out with resistant An. gambiae s.s. of M’bé and the susceptible strain, to assess the resistance level to chlorfenapyr and alphacypermethrin. Results: CDC bottle bioassays revealed a high level of resistance of An. gambiae s.s. population from M’bé to alphacypermethrin, whereas they revealed low resistance to chlorfenapyr. In experimental huts, Interceptor® G2 that was unwashed or washed 20 times killed 87% and 82% of An. gambiae s.s., respectively, whereas Interceptor® LN that was either unwashed or washed 20 times killed only about 10% of the mosquitoes. The blood-feeding inhibition induced by Interceptor® was not significantly different compared to untreated nets, whereas Interceptor® G2 that was unwashed or washed 20 times induced 42% and 34% inhibition of blood-feeding, respectively. Conclusion: Interceptor® G2 met the WHOPES criteria to undergo a phase III study. Investigation of its efficacy at a community level and the conduct of randomized controlled trials dealing with epidemiological outputs are warranted in order to study the potential of Interceptor® G2 to better protect communities.

scholarly journals Use of Insecticides in Agriculture and the Prevention of Vector-Borne Diseases: Population Knowledge, Attitudes, Practices and Beliefs in Elibou, South Côte d’Ivoire

2020 ◽  
Vol 5 (1) ◽  
pp. 36
Author(s):  
Bédjou P. N’Dri ◽  
Kathrin Heitz-Tokpa ◽  
Mouhamadou Chouaïbou ◽  
Giovanna Raso ◽  
Amoin J. Koffi ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Malaria Vectors ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Cote D’Ivoire ◽  
Attitudes And Practices ◽  
Côte D'ivoire ◽  
Population Attitudes

People’s knowledge, attitudes, practices and beliefs (KAPB) pertaining to malaria are generally well described. However, little is known about population knowledge and awareness of insecticide resistance in malaria vectors. The aim of this study was to investigate KAPB related to insecticide resistance in malaria vectors due to the use of insecticides in agriculture and the prevention against mosquitoes. In mid-2017, we carried out a cross-sectional survey in Elibou, South Côte d’Ivoire, employing a mixed methods approach. Quantitative data were obtained with a questionnaire addressed to household heads. Interviews were conducted with key opinion leaders, including village chiefs, traditional healers, heads of health centres and pesticide sellers. Focus group discussions were conducted with youth and elders. A total of 203 individuals participated in the questionnaire survey (132 males, 65%). We found that people had good knowledge about malaria and mosquitoes transmitting the disease, while they felt that preventing measures were ineffective. Pesticides were intensively used by farmers, mainly during the rainy season. Among the pesticides used, insecticides and herbicides were most commonly used. While there was poor knowledge about resistance, the interviewees stated that insecticides were not killing the mosquitoes anymore. The main reason given was that insecticides were diluted by the manufacturers as a marketing strategy to sell larger quantities. More than a third of the farmers used agricultural pesticides for domestic purposes to kill weeds or mosquitoes. We observed a misuse of pesticides among farmers, explained by the lack of specific training. In the community, long-lasting insecticidal nets were the most common preventive measure against malaria, followed by mosquito coils and insecticide sprays. The interviewees felt that the most effective way of dealing with insecticide resistance was to combine at least two preventive measures. In conclusion, population attitudes and practices related to insecticides used in agriculture and the prevention against mosquitoes could lead to resistance in malaria vectors, while people’s knowledge about insecticide resistance was limited. There is a need to raise awareness in communities about the presence of resistance in malaria vectors and to involve them in resistance management.

scholarly journals Insecticides Resistance Status of An. gambiae in Areas of Varying Agrochemical Use in Côte D’Ivoire

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Behi K. Fodjo ◽  
Benjamin G. Koudou ◽  
Emmanuel Tia ◽  
Jasmina Saric ◽  
Prisca B. N’dri ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Crop Protection ◽  
Malaria Vectors ◽  
Taqman Assay ◽  
Malaria Vector Control ◽  
Cote D’Ivoire ◽  
Côte D'ivoire ◽  
Use Pattern

Background. Insecticide resistance monitoring of the malaria vectors to different classes of insecticides is necessary for resistance management. Malaria vector control management approaches are essentially based on IRS and LLINs. However, insecticide resistance is caused by several sources of selection and in case the selection pressure is from agricultural practices, then measures need to be taken to avoid a failure of the control methods put in place. The current study was undertaken to monitor the susceptibility of vectors to different classes of insecticides in areas of varying agrochemical use patterns. Methods. A survey to determine the agricultural chemical use pattern was undertaken in ten localities across Côte d’Ivoire. In addition, WHO susceptibility tests were carried out on adults Anopheles gambiae s.l. mosquitoes emerging from collected larvae from the sites surveyed. Four insecticides from each class of the four classes of insecticides were evaluated using the standard susceptibility test methods. Furthermore, the target site mutations involved in resistance mechanisms were identified following the Taqman assay protocols and mosquito species were identified using SINE-PCR. Results. The mortalities of all the An. gambiae s.l populations were similar regardless of the pesticide use pattern. The vectors were resistant to DDT, deltamethrin, and bendiocarb in all localities. In contrast, mosquitoes showed high susceptibility to malathion. High frequency of the Kdr-West gene allele was observed (70-100%). A single Kdr-East mutation was identified in a mosquito that harboured both Ace-1 and Kdr-West genes. Conclusion. Cultivated marshlands representing good habitats for mosquito development may deeply contribute to the selection of resistance genes given the intensive use of agrochemical for crop protection. In view of these, special attention must be given to them to mitigate mosquito resistance to insecticides.

scholarly journals Influence of the agrochemicals used for rice and vegetable cultivation on insecticide resistance in malaria vectors in southern Côte d’Ivoire

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Mouhamadou S. Chouaïbou ◽  
Behi K. Fodjo ◽  
Gilbert Fokou ◽  
Ouattara F. Allassane ◽  
Benjamin G. Koudou ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Malaria Vectors ◽  
Cote D’Ivoire ◽  
Côte D'ivoire ◽  
Vegetable Cultivation

Political Stability in West African States: Problems and Solutions. (Case Studies of Côte d’Ivoire and Senegal)

2018 ◽  
pp. 75-88
Author(s):  
Lyubov Sadovskaya
Keyword(s):  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Political Stability ◽  
West African ◽  
The Political ◽  
Political Systems ◽  
African Countries ◽  
Cote D’Ivoire ◽  
External Threats ◽  
Côte D'ivoire

The article presents a new view on the problems of political stability in West African countries. For the first time was carried out a comparative analysis of the sustainability of the political systems of the two Francophone fastest growing countries in West Africa, Côte d’Ivoire and Senegal. The author analyzes the factors negatively influencing political stability social order, and those that reduce conflict potencial in these States. Internal and external threats to the political systems of Senegal and Сôte d’Ivoire are examined. The response of both countries to internal and external challenges is shown. The study proves that while external threats indanger Senegal’s political stability, such as the penetration of religious extremism, the crisis in Casamance, maritime piracy, drug traffic, for Côte d’Ivoire, on the contrary, main risks are internal: electoral, socio-political crises, the split of elites, arms smuggling, banditry. The study demonstrates that the level of social governance in Senegal is higher than in other West African countries, including Сôte d’Ivoire, due to the dualism of the political system: the coexistence of Western-style political institutions with local faiths (tariqas), as well as policy pursued by President M. Sall. aimed at achieving mutual compromise that ensure the peaceful settlement of conflicts and contradictions. The author concludes that a new approach to the development of a security strategy is required.

scholarly journals Fine scale spatial investigation of multiple insecticide resistance and underlying target-site and metabolic mechanisms in Anopheles gambiae in central Côte d’Ivoire

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Welbeck A. Oumbouke ◽  
Patricia Pignatelli ◽  
Antoine M. G. Barreaux ◽  
Innocent Z. Tia ◽  
Alphonsine A. Koffi ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Anopheles Gambiae ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Pyrethroid Resistance ◽  
Controlled Trial ◽  
Management Strategies ◽  
Cluster Randomised Controlled Trial ◽  
Cote D’Ivoire ◽  
Côte D'ivoire

Abstract Routine monitoring of occurrence, levels and mechanisms of insecticide resistance informs effective management strategies, and should be used to assess the effect of new tools on resistance. As part of a cluster randomised controlled trial evaluating a novel insecticide-based intervention in central Côte d’Ivoire, we assessed resistance and its underlying mechanisms in Anopheles gambiae populations from a subset of trial villages. Resistance to multiple insecticides in An. gambiae s.s. and An. coluzzii was detected across villages, with dose–response assays demonstrating extremely high resistance intensity to the pyrethroid deltamethrin (> 1,500-fold), and mortality following exposure to pyrethroid-treated bednets was low (< 30% mortality in cone bioassays). The 1014F kdr mutation was almost fixed (≥ 90%) in all villages but the 1575Y kdr-amplifying mutation was relatively rare (< 15%). The carbamate and organophosphate resistance-associated Ace-1 G119S mutation was also detected at moderate frequencies (22–43%). Transcriptome analysis identified overexpression of P450 genes known to confer pyrethroid resistance (Cyp9K1, Cyp6P3, and Cyp6M2), and also a carboxylesterase (COEAE1F) as major candidates. Cyp6P3 expression was high but variable (up to 33-fold) and correlated positively with deltamethrin resistance intensity across villages (r2 = 0.78, P = 0.02). Tools and strategies to mitigate the extreme and multiple resistance provided by these mechanisms are required in this area to avoid future control failures.

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