scholarly journals Role of DNA Double-strand Break Repair Genes in Cell Proliferation under Low Dose-rate Irradiation Conditions

2008 ◽  
Vol 49 (5) ◽  
pp. 557-564 ◽  
Author(s):  
Masanori TOMITA ◽  
Fumiko MOROHOSHI ◽  
Yoshihisa MATSUMOTO ◽  
Kensuke OTSUKA ◽  
Kazuo SAKAI
Keyword(s):  
Cell Proliferation ◽  
Dose Rate ◽  
Low Dose ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Low Dose Rate ◽  
Dna Double Strand Break ◽  
Repair Genes

scholarly journals The Impact of Dose Rate on DNA Double-Strand Break Formation and Repair in Human Lymphocytes Exposed to Fast Neutron Irradiation

2019 ◽  
Vol 20 (21) ◽  
pp. 5350 ◽  
Author(s):  
Shankari Nair ◽  
Monique Engelbrecht ◽  
Xanthene Miles ◽  
Roya Ndimba ◽  
Randall Fisher ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dose Rate ◽  
Neutron Irradiation ◽  
Low Dose ◽  
Human Lymphocytes ◽  
Strand Break ◽  
Double Strand Break ◽  
Low Dose Rate ◽  
Dna Double Strand Break ◽  
The Impact

The lack of information on how biological systems respond to low-dose and low dose-rate exposures makes it difficult to accurately assess the carcinogenic risks. This is of critical importance to space radiation, which remains a serious concern for long-term manned space exploration. In this study, the γ-H2AX foci assay was used to follow DNA double-strand break (DSB) induction and repair following exposure to neutron irradiation, which is produced as secondary radiation in the space environment. Human lymphocytes were exposed to high dose-rate (HDR: 0.400 Gy/min) and low dose-rate (LDR: 0.015 Gy/min) p(66)/Be(40) neutrons. DNA DSB induction was investigated 30 min post exposure to neutron doses ranging from 0.125 to 2 Gy. Repair kinetics was studied at different time points after a 1 Gy neutron dose. Our results indicated that γ-H2AX foci formation was 40% higher at HDR exposure compared to LDR exposure. The maximum γ-H2AX foci levels decreased gradually to 1.65 ± 0.64 foci/cell (LDR) and 1.29 ± 0.45 (HDR) at 24 h postirradiation, remaining significantly higher than background levels. This illustrates a significant effect of dose rate on neutron-induced DNA damage. While no significant difference was observed in residual DNA damage after 24 h, the DSB repair half-life of LDR exposure was slower than that of HDR exposure. The results give a first indication that the dose rate should be taken into account for cancer risk estimations related to neutrons.

scholarly journals Role of 53BP1 in the Regulation of DNA Double-Strand Break Repair Pathway Choice

2014 ◽  
Vol 181 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Arun Gupta ◽  
Clayton R. Hunt ◽  
Sharmistha Chakraborty ◽  
Raj K. Pandita ◽  
John Yordy ◽  
...  
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Repair Pathway ◽  
Dna Double Strand Break ◽  
Pathway Choice ◽  
Break Repair

scholarly journals Tumor Cell Kill by c-MYC Depletion: Role of MYC-Regulated Genes that Control DNA Double-Strand Break Repair

2010 ◽  
Vol 70 (21) ◽  
pp. 8748-8759 ◽  
Author(s):  
Kaisa R. Luoto ◽  
Alice X. Meng ◽  
Amanda R. Wasylishen ◽  
Helen Zhao ◽  
Carla L. Coackley ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Dna Double Strand Break ◽  
Cell Kill ◽  
Break Repair

Cell cycle-dependent protein expression of mammalian homologs of yeast DNA double-strand break repair genes Rad51 and Rad52

1997 ◽  
Vol 384 (3) ◽  
pp. 205-211 ◽  
Author(s):  
Fanqing Chen ◽  
Anthony Nastasi ◽  
Zhiyuan Shen ◽  
Mark Brenneman ◽  
Harry Crissman ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Protein Expression ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Dna Double Strand Break ◽  
Dependent Protein ◽  
Break Repair ◽  
Cycle Dependent ◽  
Repair Genes

scholarly journals The role of ATM signalling in DNA double strand break repair.

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Penelope Ann Jeggo ◽  
Aaron A Goodarzi ◽  
Atsushi Shibata ◽  
Markus Lobrich
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Dna Double Strand Break ◽  
Break Repair
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