Isolation and Identification of Vibrio nereis and Vibrio harveyi in farm raised Penaeus monodon marine shrimp

2016 ◽  
Vol 8 (4) ◽  
pp. 55-61
Keyword(s):  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Isolation And Identification ◽  
Marine Shrimp

Vibrio harveyi virulence gene expression in vitro and in vivo during infection in black tiger shrimp Penaeus monodon

2020 ◽  
Vol 139 ◽  
pp. 153-160
Author(s):  
S Peeralil ◽  
TC Joseph ◽  
V Murugadas ◽  
PG Akhilnath ◽  
VN Sreejith ◽  
...  
Keyword(s):  
Gene Expression ◽  
Virulence Genes ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
Virulence Gene ◽  
Economic Losses ◽  
Virulence Gene Expression

Luminescent Vibrio harveyi is common in sea and estuarine waters. It produces several virulence factors and negatively affects larval penaeid shrimp in hatcheries, resulting in severe economic losses to shrimp aquaculture. Although V. harveyi is an important pathogen of shrimp, its pathogenicity mechanisms have yet to be completely elucidated. In the present study, isolates of V. harveyi were isolated and characterized from diseased Penaeus monodon postlarvae from hatcheries in Kerala, India, from September to December 2016. All 23 tested isolates were positive for lipase, phospholipase, caseinase, gelatinase and chitinase activity, and 3 of the isolates (MFB32, MFB71 and MFB68) showed potential for significant biofilm formation. Based on the presence of virulence genes, the isolates of V. harveyi were grouped into 6 genotypes, predominated by vhpA+ flaB+ ser+ vhh1- luxR+ vopD- vcrD+ vscN-. One isolate from each genotype was randomly selected for in vivo virulence experiments, and the LD50 ranged from 1.7 ± 0.5 × 103 to 4.1 ± 0.1 × 105 CFU ml-1. The expression of genes during the infection in postlarvae was high in 2 of the isolates (MFB12 and MFB32), consistent with the result of the challenge test. However, in MFB19, even though all genes tested were present, their expression level was very low and likely contributed to its lack of virulence. Because of the significant variation in gene expression, the presence of virulence genes alone cannot be used as a marker for pathogenicity of V. harveyi.

scholarly journals ANALYSIS OF IMMUNE RESPONSES ON TRANSGENIC TIGER SHRIMP (Penaeus monodon) AGAINST PATHOGENIC BACTERIUM Vibrio harveyi

2014 ◽  
Vol 9 (1) ◽  
pp. 23
Author(s):  
Andi Parenrengi ◽  
Bunga Rante Tampangallo ◽  
Andi Tenriulo
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Vibrio Harveyi ◽  
Gene Transfection ◽  
Penaeus Monodon ◽  
Pathogenic Bacterium ◽  
Shrimp Culture ◽  
Tiger Shrimp ◽  
Randomized Design ◽  
Study Results

Vibriosis is one of main diseases of the black tiger shrimp Penaeus monodon infected by pathogenic bioluminous bacterium Vibrio harveyi that can cause mass mortalities in shrimp culture. The bacteria can also trigger the disease white spot syndrome virus (WSSV). An effort to produce shrimp disease-resistant strains has been done through transgenesis technology with antiviral gene transfection. By this technology, it is expected an increase in the immune response of shrimp in a variety of diseasecausing pathogens. This study aimed to determine the immune responses (total haemocytes, haemocyte differentiation, and phenoloxydase activity) of transgenic tiger shrimp against pathogenic bacterium V. harveyi. Research using completely randomized design, which consists of two treatments and three replications. Test animals being used were transgenic and non-transgenic shrimp with size, weight 3.93±1.25 g and a total length of 7.59±0.87 cm. Treatments being tested were the injection of bacterium V. harveyi (density of 5x106 cfu/mL) of 0.1 mL/individual on transgenic (A) and non-transgenic shrimp (B). Immune response parameters such as total haemocytes, haemocyte differentiation, and phenoloxydase activity were observed on day 1, 3, and 6 days after challenging. Data were analyzed using t-test by SPSS software. The results showed that the total haemocyte of transgenic shrimp was not significantly different (P>0.05) from non-transgenic shrimp, but haemocyte differentiation and phenoloxydase activity were significantly different (P<0.05) especially on sixth days after being exposed to the bioluminescent bacteria. The study results implied that transgenic shrimp has a better immune response compared than non-transgenic shrimp.

scholarly journals THE EFFECTS OF ENRICHED Moina ON THE GROWTH, SURVIVAL, AND PROXIMATE ANALYSIS OF MARINE SHRIMP (Penaeus monodon)

2021 ◽  
Vol 16 (3) ◽  
pp. 56-70
Author(s):  
WAN NURUL NADIAH WAN RASDI ◽  
◽  
MHD IKHWANUDDIN ABDULLAH ◽  
SYAMIMI AZMAN ◽  
MURNI KARIM ◽  
...  
Keyword(s):  
Penaeus Monodon ◽  
Proximate Analysis ◽  
Marine Shrimp

Molecular Docking Study of Bio-Inhibitors Extracted From Marine Macro Alga Ulva Fasciata Against Hemolysin Protein of luminescence Disease Causing Vibrio Harveyi

2021 ◽  
Author(s):  
Krishnamoorthy Sivakumar ◽  
Sudalayandi Kannappan ◽  
Balakrishnan Vijayakumar ◽  
Jithendran Karingalakkandy Poochirian ◽  
Sivamani Balasubramanian ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Tamil Nadu ◽  
Pathogenic Bacteria ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Chemotherapeutic Agents ◽  
Computer Assisted ◽  
Bacterial Disease ◽  
Molecular Docking Study ◽  
Ulva Fasciata

Abstract Shrimp grow-out and hatchery systems are being affected by bacterial disease particularly Vibrios. The use of chemotherapeutic agents in aquaculture practices has to lead to the development of resistance among aquatic bacteria. Thus, health management becomes of major importance in aquaculture. Under this situation, progressing bio-inhibitors from marine resources are most appropriate to be considered against pathogenic bacteria. Molecular docking is an appropriate tool in structural biology and computer-assisted drug design to predict and neutralize a target protein of known diseases. In this study, marine macroalga Ulva fasciata was aimed at developing inhibitors against luminescence disease-causing pathogenic bacteria Vibrio harveyi. U. fasciata was collected from the intertidal zone of Thoothukudi, Tamil Nadu, India. Extract of U. fasciata was tested against growth and virulence factors of V. harveyi during Penaeus monodon larviculture. For molecular docking, the homology modeling of virulence of hemolysin protein of V. harveyi was designed and used for docking studies against the compounds of U. fasciata as identified by GC-MS analysis. Extract of U. fasciata@200 mg mL-1 had exhibited reductions on Cumulative Percentage of Mortality (32.40%) in postlarvae against the challenge of V. harveyi infection. In docking analysis, the bio-inhibitor Methyl dehydroabietate showed the highest binding affinity among compounds docked. Statistical analysis had revealed significant differences (p<0.05) in trials. Therefore, it was proved that the bio-inhibitors from U. fasciata will be a better option for controlling luminescence disease-causing V. harveyi in shrimp grow-out practices.

Effect of the anti-lipopolysaccharide factor isoform 3 (ALFPm3) from Penaeus monodon on Vibrio harveyi cells

2012 ◽  
Vol 38 (4) ◽  
pp. 554-560 ◽  
Author(s):  
Phattarunda Jaree ◽  
Anchalee Tassanakajon ◽  
Kunlaya Somboonwiwat
Keyword(s):  
Vibrio Harveyi ◽  
Penaeus Monodon

scholarly journals ISOLASI DAN SELEKSI BAKTERI PROBIOTIK DARI LINGKUNGAN TAMBAK DAN HATCHERI UNTUK PENGENDALIAN PENYAKIT VIBRIOSIS PADA LARVA UDANG WINDU, Penaeus monodon

2016 ◽  
Vol 5 (1) ◽  
pp. 103
Author(s):  
Widanarni Widanarni ◽  
Fahmi Rajab ◽  
Sukenda Sukenda ◽  
Mia Setiawati
Keyword(s):  
Vibrio Harveyi ◽  
Penaeus Monodon

Penelitian ini bertujuan untuk memperoleh bakteri probiotik yang mampu menghambat pertumbuhan Vibrio harveyi untuk pengendalian penyakit vibriosis pada larva udang windu. Jumlah isolat bakteri yang berhasil diisolasi dari 34 sumber yang berbeda di lingkungan tambak dan hatcheri ada 118 isolat. Dari total isolat tersebut setelah diseleksi secara in vitro menggunakan metode Kirby-Bauer dipilih 3 isolat bakteri kandidat probiotik yang paling potensial dalam menghambat pertumbuhan bakteri V. harveyi MR5339, yaitu isolat 9a berasal dari Cyclotella dengan zona hambat 12,8 mm; isolat Ua berasal dari saluran pencernaan udang vaname dengan zona hambat 14,5 mm; dan isolat P17Bb berasal dari air pemeliharaan kerapu bebek dengan zona hambat 15,0 mm. Hasil uji patogenisitas dengan konsentrasi bakteri 106 CFU/mL menunjukkan bahwa ketiga isolat tersebut tidak bersifat patogen pada larva udang windu. Hasil uji tantang pada larva udang juga menunjukkan bahwa ketiga isolat tersebut mampu meningkatkan sintasan larva udang windu. Nilai sintasan larva pada perlakuan yang selain diinfeksi dengan V. harveyi MR5339 juga ditambah probiotik Ua, 9a, dan P17Bb masing-masing adalah 88,3%; 88,3%; dan 76,7% sedangkan pada perlakuan yang hanya diinfeksi dengan V. harveyi MR5339 tanpa probiotik nilai sintasannya hanya mencapai 65,0%. Populasi V. harveyi pada perlakuan dengan penambahan bakteri probiotik tampak lebih rendah dibanding perlakuan tanpa probiotik, hal ini menunjukkan kemungkinan adanya kompetisi antara bakteri V. harveyi dengan bakteri probiotik

scholarly journals Differentially Expressed Genes in Hemocytes of Vibrio harveyi-challenged Shrimp Penaeus monodon

BMB Reports ◽  
2006 ◽  
Vol 39 (1) ◽  
pp. 26-36 ◽  
Author(s):  
Kunlaya Somboonwiwat ◽  
Premruethai Supungul ◽  
Vichien Rimphanitchayakit ◽  
Takashi Aoki ◽  
Ikuo Hirono ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Differentially Expressed

scholarly journals EFEKTIVITAS LAMA PERENDAMAN BAKTERIN Vibrio harveyi TERHADAP SINTASAN DAN PERTUMBUHAN UDANG WINDU (Penaeus monodon Fabr)

2015 ◽  
Vol 10 (1) ◽  
pp. 39
Author(s):  
Arifuddin Tompo ◽  
Endang Susianingsih ◽  
Muhammad Risal
Keyword(s):  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
C 30

Penyakit pada budidaya udang dapat menyebabkan terjadinya penurunan produksi bahkan kematian pada usaha budidaya tersebut. Alternatif pencegahan yang saat ini banyak dilakukan adalah melalui immunoprofilaksis yaitu meningkatkan kekebalan udang terhadap serangan penyakit dengan pemberian imunostimulan seperti vaksin bakterin maupun vaksin rekombinan. Penelitian ini bertujuan untuk mengetahui efektivitas lama perendaman bakterin terhadap sintasan dan pertumbuhan pada udang windu (Penaeus monodon Fabr). Penelitian ini menggunakan rancangan acak lengkap dengan 5 perlakuan lama perendaman dan 4 ulangan yaitu A = kontrol (tanpa bakterin), B = Lama perendaman dengan bakterin 15 menit, C = 30 menit, D = 45 menit dan E = 60 menit dengan hewan uji benur PL-17 yang telah diperiksa bebas Vibrio dan WSSV dan padat penebaran 20 ekor/2 L air laut yang telah disterilkan. Bakterin yang digunakan dari Vibrio harveyi dengan dosis 0,2 mL/L. Uji tantang setelah pemberian bakterin dengan metode perendaman dilakukan menggunakan beberapa konsentrasi bakteri Vibrio harveyidengan 4 perlakuan dan 5 ulangan : A = penambahan bakteri 0.02 mL/L, B = penambahan bakterin 0,2 mL/L, C = penambahan bakterin 2,0 mL/L dan D = kontrol (tanpa penambahan bakteri). Peubah yang diamati adalah sintasan udang uji pada akhir penelitian. Hasil penelitian menunjukkan bahwa lama waktu perendaman menggunakan bakterin selama 45 menit dan 60 menit lebih baik jika dibandingkan dengan perendaman selama 15 dan 30 menit dengan sintasan yang dihasilkan sebesar 86,25% dan 73,75%.

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