scholarly journals Identificaiton of a clubroot resistance locus conferring resistance to a Plasmodiophora brassicae classified into pathotype group 3 in Chinese cabbage (Brassica rapa L.)

2012 ◽  
Vol 62 (3) ◽  
pp. 282-287 ◽  
Author(s):  
Takeyuki Kato ◽  
Katsunori Hatakeyama ◽  
Nobuko Fukino ◽  
Satoru Matsumoto
Keyword(s):  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Plasmodiophora Brassicae ◽  
Resistance Locus ◽  
Clubroot Resistance ◽  
Group 3

The New Clubroot Resistance Locus Is Located on Chromosome A05 in Chinese Cabbage (Brassica rapa L.)

2018 ◽  
Vol 54 (3) ◽  
pp. 296-304 ◽  
Author(s):  
M. L. Nguyen ◽  
G. F. Monakhos ◽  
R. A. Komakhin ◽  
S. G. Monakhos
Keyword(s):  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Resistance Locus ◽  
Clubroot Resistance

scholarly journals Fine Mapping a Clubroot Resistance Locus in Chinese Cabbage

2014 ◽  
Vol 139 (3) ◽  
pp. 247-252 ◽  
Author(s):  
Feng Gao ◽  
Arvind H. Hirani ◽  
Jun Liu ◽  
Zheng Liu ◽  
Guohua Fu ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Resistance Genes ◽  
Chinese Cabbage ◽  
Marker Assisted Selection ◽  
Chromosomal Region ◽  
Plasmodiophora Brassicae ◽  
Resistance Locus ◽  
Breeding Programs ◽  
Clubroot Resistance ◽  
Segregating Population

There are various clubroot pathogen (Plasmodiophora brassicae) resistance genes within Brassica species with european turnip (B. rapa ssp. rapifera) being identified as potentially the best source of resistance for the development of clubroot-resistant cultivars in chinese cabbage (B. rapa ssp. pekinensis). To use clubroot resistance genes effectively, it is necessary to map these genes so that molecular markers inside or closely linked to these resistance genes can be developed. Using molecular marker-assisted selection, the clubroot resistance genes can be effectively transferred from cultivar to cultivar and from species to species. In this report, one clubroot resistance locus was mapped on linkage group A3 using five segregating populations developed from five chinese cabbage cultivars, suggesting that all the five cultivars shared the same clubroot resistance locus. Furthermore, one of these five chinese cabbage cultivars was used to develop a large segregating population to fine-map this clubroot resistance locus to a 187-kilobp chromosomal region. Molecular markers that are closely linked to the mapped clubroot resistance locus have been developed that can be used for marker-assisted selection in chinese cabbage and canola/rapeseed (B. rapa and B. napus) breeding programs.

Transferring clubroot resistance from Chinese cabbage (Brassica rapa) to canola (B. napus)

2016 ◽  
Vol 38 (1) ◽  
pp. 82-90 ◽  
Author(s):  
Arvind H. Hirani ◽  
Feng Gao ◽  
Jun Liu ◽  
Guohua Fu ◽  
Chunren Wu ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Clubroot Resistance

scholarly journals Effect of Host Resistance and Fungicide Application on Clubroot Pathotype 6 in Green Cabbage and Napa Cabbage

2012 ◽  
Vol 22 (3) ◽  
pp. 311-319 ◽  
Author(s):  
Catarina Saude ◽  
Alan McKeown ◽  
Bruce D. Gossen ◽  
Mary Ruth McDonald
Keyword(s):  
Brassica Oleracea ◽  
Brassica Rapa ◽  
Host Resistance ◽  
Plasmodiophora Brassicae ◽  
Field Trials ◽  
Organic Soils ◽  
Southern Ontario ◽  
Clubroot Resistance ◽  
Mineral Soils ◽  
Standard Cultivar

Field trials were conducted to evaluate resistance to clubroot (Plasmodiophora brassicae, pathotype 6) in green cabbage (Brassica oleracea var. capitata) and napa cabbage (Brassica rapa ssp. pekinensis) at sites in southern Ontario in 2009 and 2010. The reaction of green cabbage cultivars Kilaton, Tekila, Kilaxy, and Kilaherb and the commercial standard cultivars, Bronco or Atlantis, were evaluated on organic (two site-years) and mineral soils (two site-years) that were naturally infested with the clubroot pathogen. In addition, fluazinam fungicide was drench applied to one treatment of the commercial standard cultivar immediately after transplanting. The napa cabbage cultivars Yuki, Deneko, Bilko, and Mirako (in 2009) and Emiko, Mirako, Yuki, and China Gold (in 2010) were evaluated only on organic soils (two site-years). At harvest, the roots of each plant were assessed for clubroot incidence and severity. Also, plant and head characteristics of the resistant green cabbage cultivars were evaluated at one site in 2010. The green cabbage cultivars Kilaton, Tekila, Kilaxy, and Kilaherb were resistant to pathotype 6 (0% to 3.8% incidence), but ‘Bronco’ was susceptible (64% to 100% incidence). Application of fluazinam reduced clubroot severity on ‘Bronco’ by 6% at one of three sites. Resistance was more effective in reducing clubroot than application of fluazinam. Plant and head characteristics of the resistant cultivars were similar to those of ‘Bronco’ treated with fluazinam. Napa cabbage cultivars Yuki, Deneko, Bilko, Emiko, and China Gold were resistant to clubroot (0% to 13% incidence), and ‘Mirako’ was highly susceptible (87% to 92% incidence). We conclude that the clubroot resistance available in several cultivars of green and napa cabbage was effective against P. brassicae pathotype 6.

scholarly journals Transcriptome and Coexpression Network Analyses Reveal Hub Genes in Chinese Cabbage (Brassica rapa L. ssp. pekinensis) During Different Stages of Plasmodiophora brassicae Infection

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuxiang Yuan ◽  
Liuyue Qin ◽  
Henan Su ◽  
Shuangjuan Yang ◽  
Xiaochun Wei ◽  
...  
Keyword(s):  
Chinese Cabbage ◽  
Plasmodiophora Brassicae ◽  
Primary Root ◽  
Resistance Mechanisms ◽  
Coexpression Network ◽  
Hub Genes ◽  
Clubroot Resistance ◽  
Network Analyses ◽  
Cortical Infection ◽  
Root Hair Infection

Clubroot, caused by the soil-borne protist Plasmodiophora brassicae, is one of the most destructive diseases of Chinese cabbage worldwide. However, the clubroot resistance mechanisms remain unclear. In this study, in both clubroot-resistant (DH40R) and clubroot-susceptible (DH199S) Chinese cabbage lines, the primary (root hair infection) and secondary (cortical infection) infection stages started 2 and 5 days after inoculation (dai), respectively. With the extension of the infection time, cortical infection was blocked and complete P. brassica resistance was observed in DH40R, while disease scales of 1, 2, and 3 were observed at 8, 13, and 22 dai in DH199S. Transcriptome analysis at 0, 2, 5, 8, 13, and 22 dai identified 5,750 relative DEGs (rDEGs) between DH40R and DH199S. The results indicated that genes associated with auxin, PR, disease resistance proteins, oxidative stress, and WRKY and MYB transcription factors were involved in clubroot resistance regulation. In addition, weighted gene coexpression network analysis (WGCNA) identified three of the modules whose functions were highly associated with clubroot-resistant, including ten hub genes related to clubroot resistance (ARF2, EDR1, LOX4, NHL3, NHL13, NAC29, two AOP1, EARLI 1, and POD56). These results provide valuable information for better understanding the molecular regulatory mechanism of Chinese cabbage clubroot resistance.

scholarly journals Mapping of a novel clubroot resistance QTL using ddRAD-seq in Chinese cabbage (Brassica rapa L.)

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Rawnak Laila ◽  
Jong-In Park ◽  
Arif Hasan Khan Robin ◽  
Sathishkumar Natarajan ◽  
Harshavardhanan Vijayakumar ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Clubroot Resistance ◽  
Resistance Qtl

scholarly journals Resistance to Plasmodiophora brassicae in Brassica rapa and Brassica juncea genotypes From China

Plant Disease ◽  
2015 ◽  
Vol 99 (6) ◽  
pp. 776-779 ◽  
Author(s):  
Hui Zhang ◽  
Jie Feng ◽  
Shujiang Zhang ◽  
Shifan Zhang ◽  
Fei Li ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Significant Interaction ◽  
Plasmodiophora Brassicae ◽  
Inbred Lines ◽  
Vegetable Crops ◽  
Highly Pathogenic ◽  
Clubroot Disease ◽  
Pak Choi ◽  
China Development

Clubroot disease, caused by Plasmodiophora brassicae Woronin, has become a major problem in cruciferous crops worldwide. Chinese cabbage (Brassica rapa), pak choi (B. rapa), and mustard (B. juncea) are important vegetable crops in China. Development of clubroot-resistant cultivars of these crops is urgently needed. In this study, 71 B. rapa and B. juncea genotypes from China, including cultivars and inbred lines, were evaluated for resistance to three P. brassicae pathotypes. A significant interaction was observed between the P. brassicae pathotypes and the Brassica genotypes. Pathotype 3, as defined on the differentials of Williams, exhibited the weakest virulence on all plant material. By contrast, pathotypes 5 and 6 were both highly pathogenic on most of the tested genotypes. In all, 10 of the 14 Chinese cabbage cultivars were resistant to all three pathotypes, while 4 were resistant only to a specific pathotype. Seven of eight progenies obtained from the selfing of Chinese cabbage cultivars were resistant to pathotype 3 but most were susceptible to pathotypes 5 and 6. Most inbred lines of Chinese cabbage and all inbred lines of pak choi and mustard were susceptible to all three pathotypes but their susceptibility was lower to pathotype 3 than to pathotypes 5 and 6.

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