scholarly journals Mixotrophic Growth of Chlorella Sp. Using Glycerol for the Production of Biodiesel: A Review

2019 ◽  
Vol 18 (2) ◽  
pp. 1-12 ◽  
Author(s):  
Sakshi Bansal
Keyword(s):  
Carbon Source ◽  
Crude Glycerol ◽  
Nitrogen Sources ◽  
Biodiesel Production ◽  
Mixotrophic Growth ◽  
Nitrogen And Phosphorus ◽  
Phosphorus Addition ◽  
Chlorella Sp ◽  
Glycerol Formation ◽  
The Relationship

Microalgae have great potential for the production of lipids that can be converted into biodiesel. Glycerol is generated as a by-product of the transesterification reaction of the lipid produced by the algae into biodiesel. The process of converting this crude glycerol into the pharmaceutical grade is expensive. Also, glycerol formation from biodiesel production creates surplus glycerol reserves. In this review, the use of crude glycerol as a carbon source for the mixotrophic growth of Chlorella Sp. is discussed. Addition of other nutritional sources like nitrogen and phosphorus was also systematically studied and the relationship between the concentration of these nutrients and the growth pattern of the algae was analyzed which is presented in the article as well.   , , Crude Glycerol, Nitrogen Sources, Phosphorus Addition

scholarly journals The effect of the nitrogen source type on the growth and consumption of crude glycerol by Streptomyces hygroscopicus CH-7

2021 ◽  
Vol 10 (1) ◽  
pp. 41-45
Author(s):  
Slavica Ilić ◽  
Jovan Ćirić ◽  
Gordana Gojgić-Cvijović
Keyword(s):  
Amino Acids ◽  
Sunflower Oil ◽  
Crude Glycerol ◽  
Biomass Concentration ◽  
Nitrogen Sources ◽  
Biodiesel Production ◽  
Streptomyces Hygroscopicus ◽  
Basic Medium ◽  
Source Type ◽  
Glycerol Consumption

In this paper we studied the effect of different amino acids (arginine, tryptophan, tyrosine, and phenylalanine) as nitrogen sources on the growth of actinomycete Streptomyces hygroscopicus CH-7 and the consumption of crude glycerol, obtained as a by-product in the biodiesel production from sunflower oil. The highest biomass concentration (9.5 g/L) was achieved using the basic medium and the medium with tryptophan (9.2 g/L), while the crude glycerol consumption was the highest in the basic medium (5.9 mg/mL) and the medium with phenylalanine (3.3 mg/mL).

scholarly journals Genome-scale metabolic modelling underscores the potential of Cutaneotrichosporon oleaginosus ATCC 20509 as a cell factory for biofuel production

2020 ◽  
Author(s):  
Nhung TT Pham ◽  
Maarten Reijnders ◽  
Maria Suarez-Diez ◽  
Bart Nijsse ◽  
Jan Springer ◽  
...  
Keyword(s):  
Carbon Source ◽  
Lipid Accumulation ◽  
Crude Glycerol ◽  
Unsaturated Fatty Acids ◽  
Biodiesel Production ◽  
Biofuel Production ◽  
Cell Factory ◽  
A Genome ◽  
Wide Range ◽  
Genome Scale

Abstract Background: Cutaneotrichosporon oleaginosus ATCC 20509 is a fast growing oleaginous basidiomycete yeast that is able to grow in a wide range of low-cost carbon sources including crude glycerol, a byproduct of biodiesel production. When glycerol is used as a carbon source, this yeast can accumulate more than 50% lipids (w/w) with high concentrations of mono-unsaturated fatty acids. Results: To increase our understanding of this yeast and to provide a knowledge base for further industrial use, a FAIR re-annotated genome was used to build a genome-scale, constraint-based metabolic model containing 1553 reactions involving 1373 metabolites in 11 compartments. A new description of the biomass synthesis reaction was introduced to account for massive lipid accumulation in conditions with high carbon to nitrogen (C/N) ratio in the media. This condition-specific biomass objective function is shown to better predict conditions with high lipid accumulation using glucose, fructose, sucrose, xylose, ethanol and glycerol as sole carbon source. Conclusion: Contributing to the economic viability of biodiesel as renewable fuel, C. oleaginosus ATCC 20509 can effectively convert crude glycerol waste streams in lipids as a potential bioenergy source. Performance simulations are essential to identify optimal production conditions and to develop and fine tune a cost-effective production process. Our model suggests ATP-citrate lyase as a target for overexpression to further improve lipid production.

scholarly journals The use of salicylaldehyde derivatives as a nitrogen source for antibiotic production by Streptomyces hygroscopicus CH-7

2017 ◽  
Vol 71 (6) ◽  
pp. 487-494
Author(s):  
Sandra Konstantinovic ◽  
Milica Zlatkovic ◽  
Jovan Ciric ◽  
Slavica Ilic ◽  
Gordana Gojgic-Cvijovic ◽  
...  
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Crude Glycerol ◽  
Antibiotic Production ◽  
Biodiesel Production ◽  
Streptomyces Hygroscopicus ◽  
Spectroscopic Methods ◽  
Salicylaldehyde Derivatives ◽  
The Media ◽  
Derivatives Of

In the present work, four derivatives of salicylaldehyde (salicylaldehyde-hydrazone, phenylhydrazone, semicarbazone and thiosemicarbazone) were synthesized using both conventional (95% ethanol) and green (crude glycerol from biodiesel production) solvents. The obtained compounds were identified by elemental microanalysis, as well as FTIR, UV/Vis and 1H-NMR spectroscopic methods. Yields of 93?98% of the compounds in crude glycerol were achieved within 10?25 min. The derivatives of salicylaldehyde and crude glycerol were used as a nitrogen and carbon source, respectively, in the medium for antibiotic (hexaene H-85 and azalomycine B) production by Streptomyces hygroscopicus CH-7. The highest concentrations of hexaene H-85 and azalomycine B were achieved in the medium containing salicylaldehyde-thiosemicarbazone (198 and 69 ?g/cm3, respectively). Derivatives of salicylaldehyde also impacted the strain morphology. In the media with salicylaldehyde- phenylhydrazone and salicylaldehyde-thiosemicarbazone, S. hygroscopicus CH-7 grew like large dispersive pellets with long twisted filaments that produced the highest yield of the antibiotics.

Production of recombinant lipase B from Candida antarctica in Pichia pastoris under control of the promoter PGK using crude glycerol from biodiesel production as carbon source

2017 ◽  
Vol 118 ◽  
pp. 123-131 ◽  
Author(s):  
Julia Macedo Robert ◽  
Fernanda Senna Lattari ◽  
Antonio Carlos Machado ◽  
Aline Machado de Castro ◽  
Rodrigo Volcan Almeida ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Carbon Source ◽  
Crude Glycerol ◽  
Candida Antarctica ◽  
Biodiesel Production ◽  
Lipase B ◽  
Recombinant Lipase

scholarly journals Genome-scale metabolic modelling underscores the potential of Cutaneotrichosporon oleaginosus ATCC 20509 as a cell factory for biofuel production

2020 ◽  
Author(s):  
Nhung TT Pham ◽  
Maarten Reijnders ◽  
Maria Suarez-Diez ◽  
Bart Nijsse ◽  
Jan Springer ◽  
...  
Keyword(s):  
Carbon Source ◽  
Lipid Accumulation ◽  
Crude Glycerol ◽  
Metabolic Model ◽  
Biodiesel Production ◽  
Biofuel Production ◽  
Cell Factory ◽  
A Genome ◽  
Wide Range ◽  
Genome Scale

Abstract Background: Cutaneotrichosporon oleaginosus ATCC 20509 is a fast growing oleaginous basidiomycete yeast that is able to grow in a wide range of low-cost carbon sources including crude glycerol, a byproduct of biodiesel production. When glycerol is used as a carbon source, this yeast can accumulate more than 50% lipids (w/w) with high concentrations of mono-unsaturated fatty acids. Results: To increase our understanding of this yeast and to provide a knowledge base for further industrial use, a FAIR re-annotated genome was used to build a genome-scale, constraint-based metabolic model containing 1553 reactions involving 1373 metabolites in 11 compartments. A new description of the biomass synthesis reaction was introduced to account for massive lipid accumulation in conditions with high carbon to nitrogen (C/N) ratio in the media. This condition-specific biomass objective function is shown to better predict conditions with high lipid accumulation using glucose, fructose, sucrose, xylose and glycerol as sole carbon source. Conclusion: Contributing to the economic viability of biodiesel as renewable fuel, C. oleaginosus ATCC 20509 can effectively convert crude glycerol waste streams in lipids as a potential bioenergy source. Performance simulations are essential to identify optimal production conditions and to develop and fine tune a cost-effective production process. Our model suggests ATP-citrate lyase as a target for further improve lipid production. Keywords: Genome-scale metabolic model; Cutaneotrichosporon oleaginosus ATCC 20509; lipid accumulation; Crude glycerol; biodiesel production; flux balance analysis; oleaginous yeast

scholarly journals The effects of nutrient stress on marine microalgae for enhancing the biodiesel production

2020 ◽  
Vol 2 (3) ◽  
pp. 27-34
Author(s):  
Marlen Trejo ◽  
Evelyn Zamudio Pérez
Keyword(s):  
Growth Rate ◽  
Green Algae ◽  
Nutrient Stress ◽  
Biodiesel Production ◽  
Hexadecanoic Acid ◽  
Undecanoic Acid ◽  
Nitrogen And Phosphorus ◽  
Chlorella Sp ◽  
Main Factors ◽  
Vital Parameters

This study aimed to investigate the effect of nutrient stress such as nitrogen and phosphorus on the growth rate of green algae, Chlorella sp. and the mechanism secreted to survive during the depletion of nutrients. The sample was tested with several vital parameters, suppressed Total Nitrogen (TN) and Total Phosphorus (TP). A process known as acclimatization was carried out to ensure that the algae can adapt to a new environment which was repeated 2 times, each taking about 2 weeks. The active compounds in the samples were measured using Gas Chromatography-Mass Spectrophotometry. The experiments showed that the control microalgae secreted hexadecanoic acid and methyl stearate while nitrogen suppressed microalgae secreted Undecanoic acid, 10-methyl- and methyl ester. Phosphorus limited microalgae secreted 1,3-Propanediamine, N-(2-aminoethyl)- due to suppress of nutrients. This experiment should be tested in several study areas in Mexico and outside Mexico to compare the productivity of green algae and the main factors that contribute to the eutrophication problem.

scholarly journals Potential of different Xanthomonas campestris strains for xanthan biosynthesis on waste glycerol from biodiesel production

2020 ◽  
Vol 24 (2) ◽  
pp. 62-66
Author(s):  
Zorana Rončević ◽  
Ida Zahović ◽  
Nikolina Danilović ◽  
Siniša Dodić ◽  
Jovana Grahovac ◽  
...  
Keyword(s):  
Xanthomonas Campestris ◽  
Crude Glycerol ◽  
Rapid Expansion ◽  
Raw Material ◽  
Biodiesel Production ◽  
Nitrogen And Phosphorus ◽  
Sustainable Solutions ◽  
Waste Glycerol ◽  
Xanthan Production ◽  
The Media

A rapid expansion of the biodiesel industry has created various ecological issues relative to crude glycerol disposal. Xanthan biosynthesis is considered one of the sustainable solutions for minimizing the adverse effects of waste crude glycerol on the environment. The initial phase of xanthan production on crude glycerol entails the screening of producing microorganism. Therefore, the purpose of this study is to examine the possibility of xanthan production on a crude glycerol-based medium using different Xanthomonas campestris strains. The bioprocesses performed were assessed according to the rheology of the media considered, amounts of xanthan produced and conversion degrees of the most important nutrients present. The pseudoplastic behavior of all the media considered, the amounts of xanthan produced (5.22-7.67 g/L) and the degrees of crude glycerol, total nitrogen and phosphorus conversion (34.44-57.61 %, 23.04-30.35 % and 18.20-22.28 %, respectively) suggest that crude glycerol, after additional bioprocess optimization, can be a suitable raw material for the industrial production of xanthan.

scholarly journals Evaluation of synthetic formaldehyde and methanol assimilation pathways in Yarrowia lipolytica

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Eija Vartiainen ◽  
Peter Blomberg ◽  
Marja Ilmén ◽  
Martina Andberg ◽  
Mervi Toivari ◽  
...  
Keyword(s):  
Carbon Source ◽  
Yarrowia Lipolytica ◽  
In Silico ◽  
Crude Glycerol ◽  
Methanol Conversion ◽  
Biodiesel Production ◽  
Formaldehyde Dehydrogenase ◽  
Dihydroxyacetone Synthase ◽  
Further Development

Abstract Background Crude glycerol coming from biodiesel production is an attractive carbon source for biological production of chemicals. The major impurity in preparations of crude glycerol is methanol, which is toxic for most microbes. Development of microbes, which would not only tolerate the methanol, but also use it as co-substrate, would increase the feasibility of bioprocesses using crude glycerol as substrate. Results To prevent methanol conversion to CO2 via formaldehyde and formate, the formaldehyde dehydrogenase (FLD) gene was identified in and deleted from Yarrowia lipolytica. The deletion strain was able to convert methanol to formaldehyde without expression of heterologous methanol dehydrogenases. Further, it was shown that expression of heterologous formaldehyde assimilating enzymes could complement the deletion of FLD. The expression of either 3-hexulose-6-phosphate synthase (HPS) enzyme of ribulose monosphosphate pathway or dihydroxyacetone synthase (DHAS) enzyme of xylulose monosphosphate pathway restored the formaldehyde tolerance of the formaldehyde sensitive Δfld1 strain. Conclusions In silico, the expression of heterologous formaldehyde assimilation pathways enable Y. lipolytica to use methanol as substrate for growth and metabolite production. In vivo, methanol was shown to be converted to formaldehyde and the enzymes of formaldehyde assimilation were actively expressed in this yeast. However, further development is required to enable Y. lipolytica to efficiently use methanol as co-substrate with glycerol.

Sign in / Sign up

Export Citation Format

Share Document