scholarly journals The effect of the nitrogen source type on the growth and consumption of crude glycerol by Streptomyces hygroscopicus CH-7

2021 ◽  
Vol 10 (1) ◽  
pp. 41-45
Author(s):  
Slavica Ilić ◽  
Jovan Ćirić ◽  
Gordana Gojgić-Cvijović
Keyword(s):  
Amino Acids ◽  
Sunflower Oil ◽  
Crude Glycerol ◽  
Biomass Concentration ◽  
Nitrogen Sources ◽  
Biodiesel Production ◽  
Streptomyces Hygroscopicus ◽  
Basic Medium ◽  
Source Type ◽  
Glycerol Consumption

In this paper we studied the effect of different amino acids (arginine, tryptophan, tyrosine, and phenylalanine) as nitrogen sources on the growth of actinomycete Streptomyces hygroscopicus CH-7 and the consumption of crude glycerol, obtained as a by-product in the biodiesel production from sunflower oil. The highest biomass concentration (9.5 g/L) was achieved using the basic medium and the medium with tryptophan (9.2 g/L), while the crude glycerol consumption was the highest in the basic medium (5.9 mg/mL) and the medium with phenylalanine (3.3 mg/mL).

scholarly journals The impact of isatin derivatives on antibiotic production by Streptomyces hygroscopicus CH-7

2016 ◽  
Vol 70 (2) ◽  
pp. 123-128
Author(s):  
Jovan Ciric ◽  
Sandra Konstantinovic ◽  
Slavica Ilic ◽  
Gordana Gojgic-Cvijovic ◽  
Dragisa Savic ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Sunflower Oil ◽  
Crude Glycerol ◽  
Antibiotic Production ◽  
Biodiesel Production ◽  
Streptomyces Hygroscopicus ◽  
The Impact ◽  
Isatin Derivatives

The effect of isatin derivatives as a nitrogen source on antibiotic (Hexaene H-85 and Azalomycine B) production by Streptomyces hygroscopicus CH-7 was studied. Isatin-3-hydrazone, 5-chloroisatin-3-hydrazone, isatin-3-tosylhydrazone, 5-chloroisatin-3-tosylhydrazone, isatin-3-(4`-hidroxy)benzoilhydrazone and 5-chloroisatin-3-(4`-hidroxy)benzoilhydrazone were synthesized in a crude glycerol, obtained during the biodiesel production from edible sunflower oil. The highest concentration of Hexaene H-85 is achieved with 5-chloroisatin-3-hydrazone (197 mg/cm3) in medium, while isatin-3-hydrazone has a greatest impact on Azalomycine B production (72 ?g/cm3).

scholarly journals Exogenous l-proline improved Rhodosporidium toruloides lipid production on crude glycerol

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Rasool Kamal ◽  
Yuxue Liu ◽  
Qiang Li ◽  
Qitian Huang ◽  
Qian Wang ◽  
...  
Keyword(s):  
Cell Growth ◽  
Lipid Content ◽  
Crude Glycerol ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Rhodosporidium Toruloides ◽  
Biodiesel Production ◽  
Lipid Yield ◽  
Stress Agent ◽  
Glycerol Consumption

Abstract Background Crude glycerol as a promising feedstock for microbial lipid production contains several impurities that make it toxic stress inducer at high amount. Under stress conditions, microorganisms can accumulate l-proline as a safeguard. Herein, l-proline was assessed as an anti-stress agent in crude glycerol media. Results Crude glycerol was converted to microbial lipids by the oleaginous yeast Rhodosporidium toruloides CGMCC 2.1389 in a two-staged culture mode. The media was supplied with exogenous l-proline to improve lipid production efficiency in high crude glycerol stress. An optimal amount of 0.5 g/L l-proline increased lipid titer and lipid yield by 34% and 28%, respectively. The lipid titer of 12.2 g/L and lipid content of 64.5% with a highest lipid yield of 0.26 g/g were achieved with l-proline addition, which were far higher than those of the control, i.e., lipid titer of 9.1 g/L, lipid content of 58% and lipid yield of 0.21 g/g. Similarly, l-proline also improved cell growth and glycerol consumption. Moreover, fatty acid compositional profiles of the lipid products was found suitable as a potential feedstock for biodiesel production. Conclusion Our study suggested that exogenous l-proline improved cell growth and lipid production on crude glycerol by R. toruloides. The fact that higher lipid yield as well as glycerol consumption indicated that l-proline might act as a potential anti-stress agent for the oleaginous yeast strain.

STUDIES ON ASCOCHYTA IMPERFECTA PECK. DIFFERENTIAL UTILIZATION OF NUTRIENTS BY ISOLATES FROM CANADIAN ALFALFA SEED

1961 ◽  
Vol 39 (7) ◽  
pp. 1591-1594 ◽  
Author(s):  
H. W. Mead
Keyword(s):  
Amino Acids ◽  
Nitrogen Sources ◽  
Basic Medium ◽  
Alfalfa Seed

Isolates of Ascochyta imperfecta which had reacted differentially to 10 leguminous hosts were grown on a basic medium amended with various sugars, amino acids, and nitrogen sources. Significant differences in the yield of mycelium on the various media showed that these isolates responded specifically to different sugars and amino acids. Among the latter, cystine at 0.025% was inhibitory to all isolates. The significance of changes in the host on development of the fungus is discussed.

scholarly journals The use of salicylaldehyde derivatives as a nitrogen source for antibiotic production by Streptomyces hygroscopicus CH-7

2017 ◽  
Vol 71 (6) ◽  
pp. 487-494
Author(s):  
Sandra Konstantinovic ◽  
Milica Zlatkovic ◽  
Jovan Ciric ◽  
Slavica Ilic ◽  
Gordana Gojgic-Cvijovic ◽  
...  
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Crude Glycerol ◽  
Antibiotic Production ◽  
Biodiesel Production ◽  
Streptomyces Hygroscopicus ◽  
Spectroscopic Methods ◽  
Salicylaldehyde Derivatives ◽  
The Media ◽  
Derivatives Of

In the present work, four derivatives of salicylaldehyde (salicylaldehyde-hydrazone, phenylhydrazone, semicarbazone and thiosemicarbazone) were synthesized using both conventional (95% ethanol) and green (crude glycerol from biodiesel production) solvents. The obtained compounds were identified by elemental microanalysis, as well as FTIR, UV/Vis and 1H-NMR spectroscopic methods. Yields of 93?98% of the compounds in crude glycerol were achieved within 10?25 min. The derivatives of salicylaldehyde and crude glycerol were used as a nitrogen and carbon source, respectively, in the medium for antibiotic (hexaene H-85 and azalomycine B) production by Streptomyces hygroscopicus CH-7. The highest concentrations of hexaene H-85 and azalomycine B were achieved in the medium containing salicylaldehyde-thiosemicarbazone (198 and 69 ?g/cm3, respectively). Derivatives of salicylaldehyde also impacted the strain morphology. In the media with salicylaldehyde- phenylhydrazone and salicylaldehyde-thiosemicarbazone, S. hygroscopicus CH-7 grew like large dispersive pellets with long twisted filaments that produced the highest yield of the antibiotics.

scholarly journals Antibiotic production by Streptomyces hygroscopicus CH-7 in medium containing Schiff base complexes

2019 ◽  
Vol 73 (2) ◽  
pp. 93-101
Author(s):  
Slavica Ilic ◽  
Sandra Konstantinovic ◽  
Gordana Gojgic-Cvijovic ◽  
Vlada Veljkovic
Keyword(s):  
Crude Glycerol ◽  
Antibiotic Production ◽  
Basal Medium ◽  
Divalent Metal ◽  
Biodiesel Production ◽  
Streptomyces Hygroscopicus ◽  
Schiff Base Complexes ◽  
Spectral Studies ◽  
Divalent Metal Ions ◽  
Modified Media

Influence of a modified media on Hexaene H-85 and Azalomycine B production by Streptomyces hygroscopicus CH-7 was investigated. The amino acid L-tryptophan, as a nitrogen source, was replaced with isatin-3-thiosemicarbazone and its complexes with some divalent metal ions. Isatin-3-thiosemicarbazone was synthesized in crude glycerol obtained as a byproduct in biodiesel production from sunflower oil. The complexes were charac-terized by elemental microanalysis and magnetic susceptibility, as well as, by Atomic absorption(AA), Fourier-transform infrared (FTIR) and Ultraviolet?visible (UV/VIS) spectro-scopic methods. The spectral studies indicated an octahedral geometry for the Mn(II), Fe(II) and Ni(II) complexes and a tetrahedral one for the Zn(II) complex. Comparing to the basal medium, isatin-3-thiosemicarbazone (ITC) and its metal complexes in the concentration of 0.5 g dm-3 showed better results in the antibiotics production. Use of medium supple-mented with the Fe(II) complex resulted in the maximum Hexaene H-85 and Azalomycine B concentrations of 306 ?g cm-3 and 127 ?g cm-3, respectively. Addition of ITC and its complexes changed the morphology of S. hygroscopicus CH-7 from filaments to pellets as a dominant shape in media resulting in higher antibiotic production.

scholarly journals Mixotrophic Growth of Chlorella Sp. Using Glycerol for the Production of Biodiesel: A Review

2019 ◽  
Vol 18 (2) ◽  
pp. 1-12 ◽  
Author(s):  
Sakshi Bansal
Keyword(s):  
Carbon Source ◽  
Crude Glycerol ◽  
Nitrogen Sources ◽  
Biodiesel Production ◽  
Mixotrophic Growth ◽  
Nitrogen And Phosphorus ◽  
Phosphorus Addition ◽  
Chlorella Sp ◽  
Glycerol Formation ◽  
The Relationship

Microalgae have great potential for the production of lipids that can be converted into biodiesel. Glycerol is generated as a by-product of the transesterification reaction of the lipid produced by the algae into biodiesel. The process of converting this crude glycerol into the pharmaceutical grade is expensive. Also, glycerol formation from biodiesel production creates surplus glycerol reserves. In this review, the use of crude glycerol as a carbon source for the mixotrophic growth of Chlorella Sp. is discussed. Addition of other nutritional sources like nitrogen and phosphorus was also systematically studied and the relationship between the concentration of these nutrients and the growth pattern of the algae was analyzed which is presented in the article as well.   , , Crude Glycerol, Nitrogen Sources, Phosphorus Addition

scholarly journals Raw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga Crypthecodinium cohnii

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 2005
Author(s):  
Patrícia Moniz ◽  
Carla Silva ◽  
Ana Cristina Oliveira ◽  
Alberto Reis ◽  
Teresa Lopes da Silva
Keyword(s):  
Crude Glycerol ◽  
Time Course ◽  
Corn Steep Liquor ◽  
Lipid Production ◽  
Biomass Concentration ◽  
Nitrogen Sources ◽  
Intact Cells ◽  
Raw Glycerol ◽  
Dry Cell Weight ◽  
The Impact

Crude glycerol, a biodiesel industry byproduct, and corn steep liquor (CSL) derived from a starch industry, were used as carbon and nitrogen sources, respectively, for lipid production, using the heterotrophic microalga C. cohnii grown in a bench bioreactor, in a batch culture. The maximum biomass concentration, lipid content and lipid productivity attained were 5.34 g/L, 24.6% (w/w Dry Cell Weight-DCW) and 0.016 g L−1 h−1, respectively. Flow cytometry analysis was used to evaluate the impact of these substrates on the microalgae cells. A high proportion of intact cells with enzymatic (esterases) activity (>50%) was present throughout the cultivation time course. These results indicate that crude glycerol and CSL can be used in the medium formulation for DHA and lipid production using this microalga, which reduce the process costs in an expected maximum of 84%.

scholarly journals Digestible energy of crude glycerol for pacu and silver catfish

2014 ◽  
Vol 44 (8) ◽  
pp. 1448-1451 ◽  
Author(s):  
Rafael Ernesto Balen ◽  
Patrick Nereu Tetu ◽  
Robie Allan Bombardelli ◽  
Paulo Cesar Pozza ◽  
Fábio Meurer
Keyword(s):  
Fish Species ◽  
Crude Glycerol ◽  
Energy Content ◽  
Biodiesel Production ◽  
Native Fish ◽  
Neotropical Fish ◽  
Digestible Energy ◽  
Silver Catfish ◽  
Glycerol Utilization ◽  
Good Potential

The increase in global biodiesel production is originating a glycerol surplus, which has no defined destination. An alternative to overcome this problem is its use as energy source in animal feeding. In Brazil, Pacu (Piaractus mesopotamicus) is one of the most farmed native fish species, whereas Silver catfish (Rhamdia quelen) is suitable for production in subtropical region. Considering little knowledge about crude glycerol utilization in feeds for Neotropical fish species, it was evaluated the apparent digestibility coefficients (ADCs) for energy of crude glycerol for P. mesopotamicus and R. quelen. The digestibility and digestible energy content of crude glycerol can be considered excellent even when compared to energy of common ingredients such as maize and wheat, presenting 0.97 and 0.89 of energy ADCs, and 15.2 and 13.95MJ kg-1 of digestible energy for Pacu and Silver catfish, respectively. In conclusion, crude glycerol is an energetic ingredient with good potential in Brazilian native fish diets.

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