Postradiation Effects of Low Intensity Electromagnetic Radiation with a Frequency of 900 MHz in Rat Liver

2020 ◽  
Vol 65 (3) ◽  
pp. 53-58
Author(s):  
M. Petrosyan ◽  
L. Nersesova ◽  
E. Karalova ◽  
A. Avetisyan ◽  
L. Abroyan ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Blood Serum ◽  
Electromagnetic Radiation ◽  
Rat Liver ◽  
Dna Content ◽  
Transcriptional Activity ◽  
Serum Creatine Kinase ◽  
Male Rats ◽  
Low Intensity ◽  
Post Radiation

Purpose: To study the changes in the activity of the liver and blood serum creatine kinase (KK) and the nucleus-nucleolus apparatus of hepatocytes of rats, subjected to the low-intensity electromagnetic radiation of 900 MHz and 25 µW/cm2 energy flux density, typical for a range of mobile phones. Material and methods: The experiments were carried out on white outbred male rats of 6 months of age, weighing 180–200g. The generator Panoramic X1-42 was used as a source of radiation having a frequency of 900 MHz. The activity of CK in the blood serum and liver extracts respectively was determined spectrophotometrically, based on the accumulation of free creatine. Using the extent of the DNA content in rat hepatocytes relative to the accepted standard, the distribution of hepatocytes in ploidy (measured in percentage poins) was detected; so was determined the ratio of eu- and aneuploid cells. Results: It appears that the two-hour single total exposure of rats causes more substantial changes in the activity levels of rats both liver and serum creatine kinase than the total time-wise comparable fractional exposure; while the enzyme exhibits signs of significant adaptation. A decrease in the average DNA content of the nucleus and nucleolus, as well as the average number of nucleolus per nucleus, along with an increase in the number of nucleolus-free nuclei after a single radiofrequency irradiation act indicates certain inhibition of the transcriptional activity of hepatocytes. At the same time, a post-radiation increase of hypodiploid cells, half of which are nucleolus-free hepatocytes, as well as an increase in the number of triploid cells, accompanied by a decrease in the number of tetraploid hepatocytes and the disappearance of hypertetraploid hepatocytes, indicate the incidence of death of a significant number of hepatocytes. Conclusion: The rat liver CK is sensitive to the action of both single low-intensity electromagnetic radiation with a frequency of 900 MHz and that dispersed through fractional exposure: the biological effect of a single exposure is more pronounced. The dynamics of the post-radiation changes occurring in the hepatocyte population upon their single radiofrequency irradiation indicates certain inhibition of the transcriptional activity of the hepatocytes, as well as the incidence of death of a significant number of hepatocytes.

scholarly journals Cord blood serum creatine kinase isoenzymes with placental dysfunction

1987 ◽  
Vol 15 (4) ◽  
pp. 350-354 ◽  
Author(s):  
Waldemar Niklinski ◽  
Zdzislaw Palynyczko ◽  
Michal Jozwik ◽  
Adam Sledziewski
Keyword(s):  
Creatine Kinase ◽  
Blood Serum ◽  
Cord Blood ◽  
Serum Creatine Kinase ◽  
Placental Dysfunction ◽  
Cord Blood Serum ◽  
Creatine Kinase Isoenzymes

Heterogeneity of serum creatine kinase isoenzyme MM in myocardial infarction: standardization of patterns by use of cord-blood serum.

1988 ◽  
Vol 34 (2) ◽  
pp. 398-401 ◽  
Author(s):  
J Williams ◽  
T Marshall
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Blood Serum ◽  
Cord Blood ◽  
Isoelectric Focusing ◽  
Sequential Sampling ◽  
Serum Creatine Kinase ◽  
Polyacrylamide Gels ◽  
Creatine Kinase Isoenzyme ◽  
Cord Blood Serum

Abstract We investigated serum creatine kinase (CK; EC 2.7.3.2) isoenzyme MM in myocardial infarction, using isoelectric focusing in polyacrylamide gels. As many as 14 sub-band species were detected, and sequential sampling revealed a progressive anodal shift in their distribution. A nomenclature for sub-band typing is proposed. It denotes the major sub-bands as 1 (pl 6.91), 2 (pl 6.65), and 3 (pl 6.35); which are usually detected in sera of normal CK range. Abnormal sub-bands (associated with increases in the concentrations of CK) are denoted as a (pl 7.55), b (pl 7.35), c (pl 7.25), d (pl 7.05), e (pl 6.85), f (pl 6.72), g (pl 6.50), h (pl 6.40), i (pl 6.28), j (pl 6.20), and k (pl 6.15). Cord-blood sera gave highly reproducible CK-MM patterns characterized by prominent detection of sub-bands 1-3 and faint detection of c, e, f, g, h, i, and j. It is recommended as a standard for CK-MM sub-band typing.

AN ANALYSIS OF AN APPARENT SEX DIFFERENCE IN THE DESOXYRIBONUCLEIC ACID CONTENT OF RAT LIVER

1975 ◽  
Vol 80 (2) ◽  
pp. 319-328
Author(s):  
R. S. Leeuwin ◽  
B. J. Visser ◽  
C. v. d. Meer
Keyword(s):  
Sex Difference ◽  
Rat Liver ◽  
Dna Content ◽  
Testosterone Propionate ◽  
Liver Extract ◽  
Extraction Procedure ◽  
Female Rats ◽  
Male Rats ◽  
Female Rat ◽  
Desoxyribonucleic Acid

ABSTRACT Using the extraction procedure of Schmidt & Thannhauser (1945) and the indole reaction for DNA according to Ceriotti (1952), the DNA content of female rat liver was about one and a half times that of male liver. Castration of male rats, with or without administration of testosterone propionate, had no effect on the liver DNA content. Spaying of female rats (5–6 weeks of age) caused a decrease of the liver DNA content. Substitution with oestradiol benzoate restored the amount of DNA. No significant sex difference was observed in the DNA content of either rat brain, kidney, spleen and thymus, or mouse liver. Dische's diphenylamine reaction showed no significant sex difference in the rat liver DNA content. It was concluded that rat liver may contain a substance which is controlled by oestrogens and which interferes with the indole reaction. The interfering factor is present in the protein fraction of the liver extract. The possible nature of this interfering substance is discussed.

scholarly journals Effect of electromagnetic radiation from a mobile phone (1745 MHz) on the condition of the reproductive system of male rats during their postnatal development

2019 ◽  
Vol 16 (2) ◽  
pp. 216-225
Author(s):  
N. V. Chueshova ◽  
F. I. Vismont ◽  
I. A. Cheshyk
Keyword(s):  
Electromagnetic Radiation ◽  
Mobile Phone ◽  
Reproductive System ◽  
Age Groups ◽  
Serum Testosterone ◽  
The Body ◽  
Male Rats ◽  
Early Puberty ◽  
Low Intensity ◽  
The Impact

A comprehensive assessment of the morphofunctional changes in the reproductive system of male rats of several age groups was carried out, starting from the prepubertal period (50–52 days) and until they reach puberty (4,5 months) under the conditions of exposure to low-intensity electromagnetic radiation from a mobile phone (EMR MP, 1745 MHz, power density of 0.2–20 µW/cm2). It was established that the nature of the revealed morphofunctional changes in the reproductive system of male rats exposed to low-intensity EMR from MT largely depends on the exposure duration and the age of animals. The impact of EMR from MT (for 7 days) on the body of male rats during puberty leads to significant changes in the developing reproductive system. Namely, against the background of an increase in the mass of epididymis and seminal vesicles, the development of degenerative changes in the testes was revealed, manifested by the inhibition of the proliferative activity and the activation of differentiation of spermatogenic epithelial cells – spermatids, which is accompanied by a significant increase in the number of epididymal spermatozoa (early puberty), while their viability decreases and serum testosterone concentrations increase. On the contrary, the prolonged (for 60 and 90 days) exposure of EMR from MT to the organism of male rats from the early puberty period and until they reach puberty is characterized by a weakly expressed spermatogenic epithelium reaction, but also the most characteristic decrease in the number and viability of spermatozoa, as well as by the increase in concentration of testosterone in blood serum. The complex of identified disorders in the morphofunctional state of the reproductive system of male rats indicates the inhibition of its function under the influence of low-intensity EMR from MT, which may be a factor affecting the decline in male fertility.

scholarly journals Influence of electromagnetic waves at frequencies of 150,176...150,664 GHz on brain microcirculation under conditions of prolonged experimental ischemia

2016 ◽  
Vol 15 (3) ◽  
pp. 64-69
Author(s):  
A. N. Ivanov ◽  
M. A. Sakhan ◽  
I. O. Bugaeva
Keyword(s):  
Electromagnetic Radiation ◽  
Chronic Cerebral Hypoperfusion ◽  
Cerebral Hypoperfusion ◽  
Male Rats ◽  
Cerebrovascular Insufficiency ◽  
Actual Problem ◽  
Flow Modulation ◽  
Low Intensity ◽  
The Impact ◽  
Brain Microcirculation

Introduction and purpose. Chronic cerebrovascular insufficiency - an actual problem of modern medicine. The key pathogenetic link of this group of diseases is a long-term violation of perfusion in the microvasculature. Currently, it is shown that low-intensity electromagnetic radiation at frequencies of millimeter and submillimeter ranges had an influence on processes in the microcirculation. The aim of this study was to examine the impact on perfusion indices in the vessels of the brain microcirculation in conditions of chronic ischemia. Materials and methods. Experiments were conducted on 52 male rats, which were divided into 3 groups. The first group consisted of 27 rats, which model of chronic cerebral hypoperfusion were reproduced. The second group consisted of 15 rats with chronic cerebral hypoperfusion and daily 30-minute exposure to low-intensity electromagnetic radiation at frequencies 150.176...150.664 GHz. 10 sham-operated rats for the analysis microcirculation mechanisms used the method of laser Doppler flowmetry were included in the third (control) group. The average perfusion, as well as active and passive mechanisms flow modulation were investigated. The main results and discussion. It was found that low-intensity electromagnetic radiation at frequencies 150.176...150.664 GHz contributes to the normalization of perfusion parameters in conditions of prolonged ischemia due to stimulation of active and passive flow modulation mechanisms. This effect is probably related to the prevention of endothelial dysfunction, as well as the influence of electromagnetic radiation on the functional activity of platelets and blood rheology. Conclusion. Thus, the results of the study suggest that low-intensity electromagnetic radiation at frequencies 150.176...150.664 GHz, is a promising method for non-drug correction of perfusion disturbances at long cerebrovascular insufficiency.

OXYDATION DES ANABOLIKUMS 1-METHYL-ANDROST-1-EN-17β-OL-3-ON MIT WASSERSTOFFTRANSFER AUF ÖSTRON

1971 ◽  
Vol 67 (3) ◽  
pp. 517-530 ◽  
Author(s):  
Martin Wenzel
Keyword(s):  
Rat Liver ◽  
Anabolic Steroid ◽  
Female Rats ◽  
Male Rats ◽  
Female Rat ◽  
Liver Slices ◽  
Androgen Effects ◽  
Biochemical Difference

ABSTRACT With the aid of metenolon-17α-T a tritium-transfer to oestrone in rat liver slices was demonstrated. This tritium-transfer from metenolon17α-T to oestrone yielding tritium-labelled oestradiol had a higher efficiency in male than in female rat liver. Correspondingly in the presence of metenolon the relation of oestrone to oestradiol is changed more in male than in female rat liver. Looking for biochemical differences between the anabolic steroid metenolon and testosterone the oxydation at C17 was measured in different organs of the rat using 17α-T-labelled steroids. The highest oxydation rate was found for both steroids in the liver. In the sexual organs of male rats the oxydation rate of testosterone was 50–10 times higher than that of the anabolic steroid. This difference was less in sexual organs of female rats. This result of a greater biochemical difference between both steroids in males than in females leads to the question, whether the dissociation between the anabolic and the androgen effects is higher in males than in females.

Biological effects of low-intensity radiofrequency fields and risk assessment for biota

2020 ◽  
Vol 60 (9) ◽  
pp. 592-596
Author(s):  
Elena I. Sarapultseva ◽  
Darya V. Uskalova ◽  
Ksenya V. Ustenko
Keyword(s):  
Radio Frequency ◽  
Electromagnetic Radiation ◽  
Electromagnetic Fields ◽  
Negative Impact ◽  
Biological Effects ◽  
High Sensitivity ◽  
Communication Technologies ◽  
Natural Ecosystems ◽  
Low Intensity ◽  
Different Levels

Despite the fact that there are still conflicting opinions about the damage caused by modern wireless communication technologies, most scientists report on the negative biological effects of low-intensity radio frequency electromagnetic radiation at different levels of the organization of live nature. There is no doubt that there is a need not only for a sanitary and hygienic assessment of man-made electromagnetic effects on humans, but also for an environmental assessment for biota. The purpose of the study was to assess the potential environmental risk of electromagnetic impact in the centimeter range on natural ecosystems. The initial data were the authors' own results in the field of radiobiology of non-ionizing radiation, as well as published of other researchers. The article analyzes the biological effects of radio frequency electromagnetic fields detected in organisms of different systematic groups and levels of organization. The data on the non-thermal biological effects of electromagnetic fields indicate a high sensitivity of different species to this factor. The analyzed research results emphasize the need to take into account the features of non-thermal effects of electromagnetic radiation on biota, since these radiations can have a negative impact on different hierarchical levels in natural ecosystems.

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