Direct Genotyping of Toxoplasma Gondii in Blood Samples from Pregnant Women in Jazan , Saudi Arabia

2015 ◽  
Vol 45 (3) ◽  
pp. 603-608
Author(s):  
Amany M. Eida
Keyword(s):  
Saudi Arabia ◽  
Toxoplasma Gondii ◽  
Pregnant Women ◽  
Blood Samples

scholarly journals Seroprevalence and risk factors of Toxoplasma gondii infection among pregnant women in south western, Saudi Arabia

2012 ◽  
Vol 38 (1) ◽  
pp. 4-10 ◽  
Author(s):  
Mona A. Almushait ◽  
Saad M. Bin Dajem ◽  
Nahla M. Elsherbiny ◽  
Mamdoh A. Eskandar ◽  
Tarik A. Al Azraqi ◽  
...  
Keyword(s):  
Risk Factors ◽  
Saudi Arabia ◽  
Toxoplasma Gondii ◽  
Pregnant Women ◽  
Toxoplasma Gondii Infection

scholarly journals Toxoplasmosis in humans and animals in Saudi Arabia: A systematic review

2020 ◽  
Vol 14 (08) ◽  
pp. 800-811
Author(s):  
Khalil Mohamed
Keyword(s):  
Systematic Review ◽  
Saudi Arabia ◽  
Toxoplasma Gondii ◽  
Pregnant Women ◽  
Chronic Infection ◽  
Educational Programs ◽  
Current Article ◽  
Almost All ◽  
Future Work ◽  
Different Parts

Introduction: The causative agent of toxoplasmosis is Toxoplasma gondii (T. gondii); an intracellular obligate parasite causes abortion in humans and animals. The review aims to clarify the situation of the disease in humans and animals in different parts of the country so that data will be available for any future work regarding the control of the disease. Methodology: All humans and animal research studies published in the last 18 years between 2000 and 2018 in Saudi Arabia were targeted, including prevalence or seroprevalence of T. gondii infection or antibodies. The searched strategy included human or pregnant women or children as well as animals or any particular species. Results: The result showed that approximately one-third of the population in Saudi Arabia had IgG seropositivity, and 6.4% had IgM seropositivity. Moreover, the disease was widespread in almost all the regions except AL- Jouf, where no data published. The prevalence of the chronic infection was high in sheep in Riyadh 68%, and the prevalence of acute was found in Najran 19% in sheep, goats, and camels. Conclusions: The current article showed the importance of the disease in Saudi Arabia for both humans and animals. The educational programs should be established to impart people to avoid the infection by the parasite.

scholarly journals Detection of Acute and Chronic Toxoplasma gondii Infection among Women with History of Abortion in the Southwest of Iran

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Jasem Saki ◽  
Maryam Zamanpour ◽  
Mahin Najafian ◽  
Niloofar Mohammadpour ◽  
Masoud Foroutan
Keyword(s):  
Toxoplasma Gondii ◽  
Pregnant Women ◽  
Umbilical Cord ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Avidity ◽  
Normal Delivery ◽  
Blood Samples ◽  
Igg Avidity ◽  
Avidity Test ◽  
History Of

Background. Toxoplasma gondii (T. gondii) is one of the most common intracellular protozoan parasites, which can infect humans and a wide range of mammals and birds. The current study is aimed at investigating the occurrence of T. gondii infection in women with a history of abortion in Khuzestan, Iran. Materials and Methods. A total of 480 women with an abortion history, as well as 200 pregnant women with a normal delivery, were examined in this study. The blood, placenta, and umbilical cord blood samples were assessed by the enzyme-linked immunosorbent assay (ELISA) and nested-polymerase chain reaction (PCR) assay. Results. Based on the results of ELISA assay, the prevalence of toxoplasmosis was 30.83% in women with a history of abortion (25.62% with T. gondii IgG and 5.20% with T. gondii IgM). According to the IgG avidity test, 60.16% of IgG-positive samples showed high avidity, while 27.64% showed low avidity. On the other hand, the prevalence of toxoplasmosis in women with a normal delivery was 23% (21.5% with T. gondii IgG and 1.5% with T. gondii IgM). According to the IgG avidity test, 81.39% of these women showed high avidity, while only 4.65% showed low avidity. Based on the nested-PCR method, T. gondii DNA was detected in 14.18% of blood samples, 4.69% of placental samples, and 1.34% of umbilical cord samples, collected from 148 seropositive women with a history of abortion. Besides, using this method, the parasite DNA was identified in 4.34% of blood samples, collected from 46 seropositive women with a normal delivery, but not in any of the umbilical cord or placenta samples. Conclusion. The present results showed that T. gondii infection contributes to abortion in Khuzestan Province, Iran. Therefore, it is essential to investigate toxoplasmosis in pregnant women, especially in those who are seronegative, using molecular and serological methods and inform them about their disease and the associated risks.

scholarly journals Anti-Toxoplasma gondii Antibodies: Prevalence and Risk Factors among Pregnant Women Accessing Antenatal Care in Some Primary Health Centers in Jos Metropolis, Nigeria

2021 ◽  
pp. 9-17
Author(s):  
Ocheme Julius Okojokwu ◽  
Innocent Ajegba Onaji ◽  
Entonu Elijah Entonu ◽  
Bashiru Abubakar ◽  
Maryam Adebayo ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Antenatal Care ◽  
Pregnant Women ◽  
Public Health Problem ◽  
High Rate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Igm Antibody ◽  
Primary Health

Toxoplasma gondii infection causes high rate of gestational and congenital infection across the globe and is considered a both a public health problem and a neglected disease. The study was carried out to determine the prevalence of anti-Toxoplasma gondii antibodies and the associated risk factors among pregnant women attending antenatal care in some Primary Health Centres in Jos, Plateau State, Nigeria. In this cross-sectional study carried out within 5 months between Jan – May 2019, a total of 182 blood samples were collected from consenting pregnant women. Structured questionnaire was used to obtain data on socio-demography and risk factors. Three millilitres (3 ml) of blood samples were collected from the study participants. Sera were separated from the blood and evaluated for anti-Toxoplasma gondii antibodies (IgG and IgM) using enzyme-linked immunosorbent assay (ELISA). The data collected from the experiment were analysed using Statistical Package for the Social Sciences. Out of the 182 samples examined, 84 (46.2%) had anti-Toxoplasma gondii IgG antibody, 2 (1.1%) had IgM antibody while 98 (53.4%) were neither seropositive for IgG nor IgM. Trimester of pregnancy was significantly associated with prevalence of anti-Toxoplasma IgM antibody. In conclusion, Toxoplasmosis is prevalent in Jos. Eleven out of every 1000 women (i.e. 1.1%) had recent toxoplasmosis and 53.4% were not protected against primary infection thereby underscoring the need for prevention and control during pregnancy through enlightenment.

scholarly journals Detection of Toxoplasma gondii by Loop-mediated Isothermal Amplification in Blood and Urine Samples from Women in Saudi Arabia

2020 ◽  
Author(s):  
Elsamoual Ibrahim Ahmedani ◽  
Atif A Elagib ◽  
Khalil Mohamed ◽  
Jawahir Alghamdi ◽  
Akbar Ali ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Toxoplasma Gondii ◽  
Molecular Techniques ◽  
Isothermal Amplification ◽  
Urine Samples ◽  
Categorical Variables ◽  
Female Population ◽  
Blood Samples ◽  
Loop Mediated Isothermal Amplification ◽  
Blood And Urine Samples

Introduction: Toxoplasmosis is the disease which is caused by the protozoan parasite Toxoplasma gondii (T. gondii), which has the ability to infect all warm-blooded animals. Several molecular techniques for the diagnosis of Toxoplasma gondii includes normal and Real Time Polymerase Chain Reaction (RT-PCR), and gene sequencing. Aim: To apply Loop-Mediated Isothermal Amplification (LAMP) for the diagnosis of T. gondii infections in urine and blood samples from female population in Saudi Arabia. Materials and Methods: In this cross-sectional study, genomic DNA was extracted from 22 blood and urine samples (11 each), two LAMP assays based on B1 and Surface Antigen 2 (SAG2) genes of T. gondii was performed. Conventional PCR was done for the LAMP product followed by sequencing to confirm the specificity of LAMP method. Statistical Package for the Social Sciences (SPSS) version 20.0 was used to summarise continuous and categorical variables. Results: From 22 samples, 17 samples were LAMP positive in both urine and blood samples, four were negative in blood and positive in urine samples, and one was positive in blood and negative in urine. Sequencing of PCR product confirm the specificity of the method used. Conclusion: LAMP detection of T. gondii DNA is an appropriate, sensitive and specific method for diagnosis of toxoplasmosis from urine and blood specimens in humans.

scholarly journals Prevalence and genotyping of Toxoplasma gondii among Saudi pregnant women in Saudi Arabia

2016 ◽  
Vol 24 (6) ◽  
pp. 645-651 ◽  
Author(s):  
Jawahir Alghamdi ◽  
Maha Hussein Elamin ◽  
Samia Alhabib
Keyword(s):  
Saudi Arabia ◽  
Toxoplasma Gondii ◽  
Pregnant Women

Comparison of Salivary Antioxidants and Oxidative Stress Status in Gestational Diabetes Mellitus and Healthy Pregnant Women

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Ahmadi-Motamayel ◽  
Shima Fathi ◽  
Mohammad Taghi Goodarzi ◽  
Shiva Borzouei ◽  
Jalal Poorolajal ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Pregnant Women ◽  
Oxidative Stress Markers ◽  
Blood Samples ◽  
Stress Markers ◽  
Total Antioxidant ◽  
Total Oxidative Stress ◽  
And Oxidative Stress

Background: One of the most common complications of pregnant women is gestational diabetes mellitus (GDM). Oxidative stress can play an important role in GDM. Objective: The aim of this study was to evaluate salivary antioxidants and oxidative stress markers in GDM. Method: Twenty pregnant women with GDM and 20 healthy pregnant women with normal blood glucose test participated in this study. Five mL of unstimulated saliva samples were collected. Spectrophotometric assay was carried out for sialochemical analysis. Stata software was used for data analysis. Results: The GDM group exhibited no significant difference in salivary total antioxidant capacity and malondialdehyde compared to the healthy control group. All of antioxidants markers, the uric acid, total antioxidant, peroxidase and catalase, decreased in GDM group that the difference of peroxidase and catalase was statistically significant. All of oxidative stress markers, the salivary malondyaldehid, total oxidative stress and total thiol, increased in GDM group. GDM group exhibited significantly higher salivary total oxidative stress levels. Conclusion: Catalase level was significantly lower and total oxidative stress was significantly higher. These two markers might have significant importance and might exhibit early changes compared to other factors in GDM. . Some of salivary antioxidants might have diagnostic, prognostic or therapeutic implications in GDM. Other studies with large sample size on salivary and blood samples need to be done to confirm this properties and salivary samples using instead of blood samples in GDM biomarkers changes.

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