scholarly journals DNA-based detection of Leptospira wolffii, Giardia intestinalis and Toxoplasma gondii in environmental feces of wild animals in Korea

2021 ◽  
Author(s):  
Priyanka KUMARI ◽  
Kyung Yeon EO ◽  
Woo-Shin LEE ◽  
Junpei KIMURA ◽  
Naomichi YAMAMOTO
Keyword(s):  
Toxoplasma Gondii ◽  
Giardia Intestinalis ◽  
Wild Animals

Development of a qRT-PCR method to assess the viability of Giardia intestinalis cysts, Cryptosporidium spp. and Toxoplasma gondii oocysts

Food Control ◽  
2016 ◽  
Vol 59 ◽  
pp. 359-365 ◽  
Author(s):  
Emmanuelle Travaillé ◽  
Stéphanie La Carbona ◽  
Gilles Gargala ◽  
Dominique Aubert ◽  
Karine Guyot ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Giardia Intestinalis ◽  
Qrt Pcr ◽  
Cryptosporidium Spp ◽  
Pcr Method

scholarly journals Molecular identification of Toxoplasma gondii in roadkill wild animals in Yucatan, Mexico

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Marco Antonio Torres-Castro ◽  
Rodrigo Adán Medina-Pinto ◽  
Henry René Noh-Pech ◽  
Fernando I. Puerto ◽  
Roger Iván Rodríguez-Vivas
Keyword(s):  
Toxoplasma Gondii ◽  
Animal Health ◽  
Negative Control ◽  
Molecular Evidence ◽  
Wild Animals ◽  
Intermediate Hosts ◽  
Nested Polymerase Chain Reaction ◽  
Muscle Sample ◽  
Wild Mammals ◽  
Lung Sample

Toxoplasma gondii is an obligate intracellular protozoan parasite, recognized as the etiologic agent of toxoplasmosis, a zoonotic endemic disease in several countries, including Mexico. In the Yucatan State of Mexico, Toxoplasma infection has a high impact in both human and domestic animal health. Wild animals can also host zoonotic pathogens such as Toxoplasma gondii. The presence of Toxoplasma gondii DNA in roadkill wild animals in Yucatan was detected using a nested Polymerase Chain Reaction. Toxoplasma gondii DNA was identified in several organs retrieved from a Yucatan squirrel (Sciurus yucatanensis), a coatimundi (Nasua narica), and a greater grison (Galictis vittata). The amplified fragments of Toxoplasma gondii DNA were purified, sequenced, and certified by BLAST analysis. Our results confirm that Toxoplasma gondii can infect wild mammals from Yucatan, which could act as intermediate hosts and contribute to the transmission of the disease to humans and domestic animals, as well as other wild animal species. We present the first molecular evidence of Toxoplasma gondii in a squirrel and a coatimundi from Yucatan, and quite possibly in a greater grison at a global level.Figure 1. Agarose gel presenting PCR amplicons (560 bp) positive to Toxoplasma gondii. 1) C+: positive control; A: Yucatan squirrel liver sample; B: great grison femoral muscle sample; C: coatimundi kidney sample; C-: negative control. 2) A: Yucatan squirrel brain sample; B: great grison lung sample; C-: negative control.

scholarly journals Toxoplasma gondii in domestic and wild animals from forest fragments of the municipality of Natal, northeastern Brazil

2014 ◽  
Vol 23 (4) ◽  
pp. 501-508 ◽  
Author(s):  
Gislene Fátima da Silva Rocha Fournier ◽  
Marcos Gomes Lopes ◽  
Arlei Marcili ◽  
Diego Garcia Ramirez ◽  
Igor Cunha Lima Acosta ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Monodelphis Domestica ◽  
Northeastern Brazil ◽  
Forest Fragments ◽  
Wild Animals ◽  
Domestic Cats ◽  
Tissue Samples ◽  
Wild Mammals ◽  
Modified Agglutination Test ◽  
Artibeus Lituratus

Toxoplasmosis stands out as a global disease that has felines as definitive hosts. In the municipality of Natal, Rio Grande do Norte State, Brazil, two parks are notable for their ecological and social importance. This study aimed to investigate the presence of Toxoplasma gondii in short hair cats, bats and small non-volant mammals in these two ecological reserves. Altogether, biological samples were obtained from 154 mammals, 92 wild animals from both areas and 62 domestic cats of the Parque da Cidade. In total, 22 (53.7%) non-volant wild mammals, 11 (21.5%) bats and 28 (52.8%) cats were positive for IgG anti-T. gondii antibodies using the Modified Agglutination Test (≥ 25). It was possible to detect the presence of T. gondii DNA, by means of a molecular amplification of a B1 gene fragment (155bp), in 92 tissue samples from wild animals, including Didelphis albiventris, Monodelphis domestica, Artibeus lituratus, Carollia perspicillata and Glossophaga soricina. Of the 62 cats examined by the same molecular method, T. gondii DNA could be detected in 4 cats. In this study, it was observed the circulation of T. gondii in wild species and domestic cats, demonstrating the involvement of wild and domestic animals in the cycle of T. gondii.

High seroprevalence of antibodies to Toxoplasma gondii in wild animals from Portugal

2010 ◽  
Vol 108 (5) ◽  
pp. 1163-1169 ◽  
Author(s):  
Ana Patrícia Lopes ◽  
Roberto Sargo ◽  
Manuela Rodrigues ◽  
Luís Cardoso
Keyword(s):  
Toxoplasma Gondii ◽  
Wild Animals ◽  
High Seroprevalence

Detection of Blood Parasites and Toxoplasma gondii in Wild Animals in Dinder National Park, Sudan

2021 ◽  
Vol 11 (2) ◽  
pp. 157-170
Author(s):  
Aisha Elfaki Mohamed ◽  
◽  
Manal Yosif Ishag ◽  
Mohamed Siyab Eldin Ahmed ◽  
◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
National Park ◽  
Blood Parasites ◽  
Wild Animals

Seroprevalence of Toxoplasma gondii in Domestic and Wild Animals From the Fernando de Noronha, Brazil

2012 ◽  
Vol 98 (3) ◽  
pp. 679-680 ◽  
Author(s):  
D. G. C. Costa ◽  
M. F. V. Marvulo ◽  
J. S. A. Silva ◽  
S. C. Santana ◽  
F. J. R. Magalhães ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Wild Animals ◽  
Fernando De Noronha

Genotyping of Giardia intestinalis from domestic and wild animals in Japan using glutamete dehydrogenase gene sequencing

2005 ◽  
Vol 133 (4) ◽  
pp. 283-287 ◽  
Author(s):  
Tadashi Itagaki ◽  
Shizuka Kinoshita ◽  
Mikiko Aoki ◽  
Naoyuki Itoh ◽  
Hideharu Saeki ◽  
...  
Keyword(s):  
Gene Sequencing ◽  
Giardia Intestinalis ◽  
Wild Animals ◽  
Dehydrogenase Gene

scholarly journals Prevalence of Toxoplasma gondii infection among small mammals in Tatarstan, Russian Federation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nikolai D. Shamaev ◽  
Eduard A. Shuralev ◽  
Oleg V. Nikitin ◽  
Malik N. Mukminov ◽  
Yuriy N. Davidyuk ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Russian Federation ◽  
Small Mammals ◽  
Rural Areas ◽  
Infection Prevalence ◽  
Wild Animals ◽  
Intermediate Hosts ◽  
Mammal Species ◽  
B1 Gene ◽  
Toxoplasma Gondii Infection

AbstractToxoplasma gondii is a zoonotic parasite with a wide host range that includes humans, domestic animals and wild animals. Small mammals serve as intermediate hosts for T. gondii and may contribute to the persistence of this parasite in the environment. Mass mortality in wild animals and deaths in rare endemic species make the study of this parasite of growing importance. In this study, T. gondii infection prevalence was evaluated in brain tissues from 474 small mammals captured at 26 trapping points in urban and rural areas of Tatarstan, Russian Federation. Nested PCR was used to detect the T. gondii B1 gene in the samples. Overall, 40/474 samples (8.44%) showed B1 gene positivity. T. gondii infection among the wild small mammals trapped in the rural area was significantly higher as a whole than that of the urban area as a whole. Multivariate logistical regression analysis also showed that the trapping area (rural or urban) significantly contributed to T. gondii positivity. Vegetation in the trapping points, small mammal species, sex, age or distance from the trapping points to the nearest human settlements did not significantly affect T. gondii positivity in the sampled small mammals.

scholarly journals Detection of anti-Toxoplasma gondii antibodies in wild free-living birds and mammals from the northwest region of São Paulo state, Brazil

2021 ◽  
Vol 58 ◽  
pp. e176683
Author(s):  
Lilian Castiglioni ◽  
Luiz Paulo Nogueira Aires ◽  
Vinícius Matheus Ferrari ◽  
Fernando Henrique Antunes Murata ◽  
Herbert Sousa Soares ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Animal Species ◽  
Sao Paulo ◽  
São Paulo ◽  
Wild Animals ◽  
Free Living ◽  
Paulo State ◽  
Two Samples ◽  
São Paulo State ◽  
Northwest Region

Toxoplasmosis is a protozoonosis caused by an obligate intracellular parasite named Toxoplasma gondii, which can infect humans and a large number of homeothermic animal species with worldwide distribution. The present study aimed to detect anti T. gondii antibodies from serological samples of free living wild animals from the northwest region of São Paulo state, Brazil. Thirty-two samples (eight from birds and 24 from mammals) were analyzed by the modified agglutination test (MAT) using 5 cut-off points for birds and 25 for mammals. Seropositivity was observed in 25% (2/8) of birds, including the species Rupornis magnirostris (roadside hawk) and Caracara plancus (southern caracara), and 29.2% (7/24) animals were seropositive among mammals, including one hoary fox (Lycalopex vetulus), two maned wolves (Chrysocyon brachyurus), one black howler monkey (Alouatta caraya), two crab-eating foxes (Cerdocyon thous) and one gray brocket deer (Mazama gouazoubira). The results obtained with the present study indicate the exposure to T. gondii of free-living wild animals from the northwest region of São Paulo state and, therefore, that they probably play a role in the transmission and maintenance of T. gondii in the environment they inhabit. Thus, identification of the infection in several animal species in the region indicates the environmental contamination of the area. Studies of this nature may help to understand the importance of the prevention and control of this disease in Brazil.

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