PUBLIC HEALTH IMPACT OF RAPID IDENTIFICATION OF EPIDEMIOLOGICALLY IMPORTANT CHARACTERISTICS OF SALMONELLA SPP. BY MALDI-TOF MS

Introduction: Infections caused by Salmonella are an ongoing worldwide public health problem, often found as a source of nosocomial infections, which cause significant socio-economic burdens. Salmonella is a major food-borne pathogen causing primarily gastrointestinal diseases as well as other localized and systemic infections and extraintestinal complications. MALDI-TOF MS is a new method used by clinical laboratories for rapid, reliable, cost-effective and user-friendly diagnosis of many medical important bacteria of public health interest. The use of this technique improves early identification of genus Salmonella on the species, subspecies and even serovar level, which has a positive impact on public health. Objectives: The aim of the study was to evaluate the importance of MALDI-TOF mass spectrometry for rapid identification of epidemiologically important Salmonella serovars. Based on the latest knowledge about specific biomarker molecules the possibility to identify Salmonella enterica subsp. enterica serovar Enteritidis was verified, which is one of the most common serovars present in Europe associated with gastrointestinal diseases. For serovar Enteritidis a unique mass peak at m/z 6,036 was used. Methods: 140 clinical Salmonella isolates were collected from January to October 2017. Serotyping of Salmonella species, subspecies and serovars was performed by slide agglutination technique: 139 isolates were identified as Salmonella enterica subsp. enterica and one isolate as Salmonella enterica subsp. diarizonae (IIIb). From 139 isolates of Salmonella enterica subsp. enterica the following serovars were detected: 108 Enteritidis, 12 Typhimurium, 6 Infantis, 3 Agona, 3 Derby, 7 others. All isolates were identified also by MALDI-TOF MS as Salmonella spp. For all isolates a unique mass peak at m/z 6.036 was used, which is considered to be relevant for serovar Enteritidis according to the most recent known data. Results: 103 isolates from a total of 108 slide agglutination positive isolates for serovar Enteritidis showed a specific mass signal at m/z 6,036 (+/-). 5 isolates did not contain this specific protein. After repeated analysis from re-culture, the specific protein was found also in the remaining 5 isolates. 32 serovars other than Enteritidis did not contain this specific biomarker molecule. Conclusion: We can confirm that MALDI-TOF MS is a rapid and reliable method to identify of the most common serovar Salmonella Enteritidis based on the diagnostic marker peak at m/z 6,036 identified by recent studies. This unique mass signal showed 100% specificity and 95% sensitivity for Enteritidis serovar in our study. We can conclude that the determination of this frequently present serovar is significantly accelerated by MALDI-TOF MS. The rapid and reliable diagnosis is important for the early treatment and prevention of the spread of salmonellosis with a positive impact on public health.

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Prevalencia de Salmonella spp., en ganglios mesentéricos de porcinos en plantas de beneficio Colombianas

Revista Mvz Córdoba ◽

10.21897/rmvz.1242 ◽

2018 ◽

pp. 6447-6486

Author(s):

Carlos Ayala-Romero ◽

Carlos Ballen-Parada ◽

Mónica Rico-Gaitan ◽

Ileana Chamorro-Tobar ◽

Diana Zambrano-Moreno ◽

...

Keyword(s):

Salmonella Spp ◽

Maldi Tof Ms ◽

Maldi Tof ◽

El Sistema ◽

Tof Ms

Objetivo. El objetivo fue determinar la prevalencia de Salmonella spp., en ganglios mesentéricos de porcinos, provenientes de diferentes regiones de Colombia. Materiales y Métodos. Se realizó un muestreo estratificado por fijación proporcional en plantas de beneficio, de cada uno de los 13 departamentos participantes, cuyo volumen de producción de carne de cerdo es representativo a nivel nacional. El muestreo se realizó durante cinco meses, para un total de 457 muestras analizadas. La identificación de Salmonella spp., se realizó mediante el Sistema Molecular MDS, luego los aislamientos fueron confirmados por Maldi-TOF MS. Se determinó la susceptibilidad antimicrobiana de los aislamientos usando el panel B1016-180 y el análisis estadístico se realizó en Whonet 2016, posteriormente algunos de los aisalmientos multi-resistentes fueron serotipificados por el método de Kauffman-White. Resultados. La prevalencia nacional fue 28.2%, con presencia de los serotipos S. Typhimurium, S. Agama, S. London, S. Agona, S. Haifa y S. 1,4,12: i : --. Se encontró resistencia a antibióticos de uso frecuente en humanos (23.6% Trimetoprim/Sulfametoxazol, 2.7% Cefotaxime (CTX), 11.8% Ampicilina (AMP) y 1.8% Ciprofloxacina). Conclusión. La prevalencia de Salmonella en ganglios mesénticos fue del 28.2%, siendo la región del Huila la que más aportó, se recuperaron serotipos atípicos como S. London y S. Haifa

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MALDI TOF MS for the identification of Salmonella spp. from swine

Korean Journal of Veterinary Service ◽

10.7853/kjvs.2016.39.4.247 ◽

2016 ◽

Vol 39 (4) ◽

pp. 247-251

Author(s):

Jun Hyung Sohn ◽

Woo Jin Jeon ◽

Young Mi Lee ◽

Seon Soo Kim

Keyword(s):

Salmonella Spp ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

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Theoretical ribosomal protein mass distribution of Pseudomonas aeruginosa PAO1

10.7287/peerj.preprints.3500 ◽

2018 ◽

Author(s):

Wenfa Ng

Keyword(s):

Pseudomonas Aeruginosa ◽

Ribosomal Protein ◽

Ribosomal Proteins ◽

Small Subunit ◽

Structure And Function ◽

Maldi Tof Ms ◽

Maldi Tof ◽

And Function ◽

Unique Mass ◽

Tof Ms

Ribosomes are the protein synthesis factories of a cell and thus are evolutionary conserved in structure and function. Comprising a large and small subunit, the ribosome is further made up of ribosomal proteins that give structure and function to different parts of the macromolecular complex. Current methods for isolating the ribosome include density gradient ultracentrifugation that separates the ribosome into the large and small subunit. Separation of the various ribosomal proteins that comprise each of the subunit would require a solubilization step followed by the use of sodium dodecyl sulphate and polyacrylamide gel electrophoresis (SDS-PAGE). However, possibility exists for the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to profile the set of ribosomal proteins that could be solubilized from each ribosome subunit. Using ribosomal protein amino acid sequence information from Kyoto Encyclopaedia of Genes and Genomes (KEGG), the molecular weight of each ribosomal protein from Pseudomonas aeruginosa PAO1 was calculated in this report. Obtained results revealed that each ribosomal protein had a unique mass that could be detected by mid-range MALDI-TOF MS instruments. More importantly, the mass of ribosomal proteins constitutes a unique mass fingerprint of each ribosome subunit, which accounts for the different structure and functions of the large and small ribosome subunit. Overall, current mass resolution of MALDI-TOF MS instruments could resolve ribosomal proteins and thus provides a tool for profiling the set of ribosomal proteins that constitute the large and small subunit of the ribosome.

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A Side by Side Comparison of Bruker Biotyper and VITEK MS: Utility of MALDI-TOF MS Technology for Microorganism Identification in a Public Health Reference Laboratory

PLoS ONE ◽

10.1371/journal.pone.0144878 ◽

2015 ◽

Vol 10 (12) ◽

pp. e0144878 ◽

Cited By ~ 68

Author(s):

Simon Lévesque ◽

Philippe J. Dufresne ◽

Hafid Soualhine ◽

Marc-Christian Domingo ◽

Sadjia Bekal ◽

...

Keyword(s):

Public Health ◽

Reference Laboratory ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Microorganism Identification ◽

Vitek Ms ◽

Tof Ms

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Application of MALDI-TOF MS Systems in the Rapid Identification of Campylobacter spp. of Public Health Importance

Journal of AOAC International ◽

10.5740/jaoacint.17-0266 ◽

2018 ◽

Vol 101 (3) ◽

pp. 761-768 ◽

Cited By ~ 7

Author(s):

Ying-Hsin Hsieh ◽

Yun F Wang ◽

Hercules Moura ◽

Nancy Miranda ◽

Steven Simpson ◽

...

Keyword(s):

Public Health ◽

Rapid Identification ◽

Molecular Diagnostic ◽

Molecular Diagnostic Techniques ◽

Public Health Importance ◽

Intact Cells ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Campylobacter Spp ◽

Tof Ms

Abstract Campylobacteriosis is an infectious gastrointestinal disease caused by Campylobacter spp. In most cases, it is either underdiagnosed or underreported due to poor diagnostics and limited databases. Several DNA-based molecular diagnostic techniques, including 16S ribosomal RNA (rRNA) sequence typing, have been widely used in the species identification of Campylobacter. Nevertheless, these assays are time-consuming and require a high quality of bacterial DNA. Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) MS is an emerging diagnostic technology that can provide the rapid identification of microorganisms by using their intact cells without extraction or purification. In this study, we analyzed 24 American Type Culture Collection reference isolates of 16 Campylobacter spp. and five unknown clinical bacterial isolates for rapid identification utilizing two commercially available MADI-TOF MS platforms, namely the bioMérieux VITEK® MS and Bruker Biotyper systems. In addition, 16S rRNA sequencing was performed to confirm the species-level identification of the unknown clinical isolates. Both MALDI-TOF MS systems identified the isolates of C. jejuni, C. coli, C. lari, and C. fetus. The results of this study suggest that the MALDI-TOF MS technique can be used in the identification of Campylobacter spp. of public health importance.

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Occurrence of Listeria monocytogenes in Artisanal Cheeses from Poland and Its Identification by MALDI-TOF MS

Pathogens ◽

10.3390/pathogens10060632 ◽

2021 ◽

Vol 10 (6) ◽

pp. 632

Author(s):

Renata Pyz-Łukasik ◽

Michał Gondek ◽

Dagmara Winiarczyk ◽

Katarzyna Michalak ◽

Waldemar Paszkiewicz ◽

...

Keyword(s):

Public Health ◽

Listeria Monocytogenes ◽

Food Industry ◽

Foodborne Pathogen ◽

Safety Criterion ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Source Of Infection ◽

Potential Public Health ◽

Tof Ms

Listeria monocytogenes is a foodborne pathogen. A source of infection can be artisanal cheeses. Identification of the Listeria species is important for the protection of public health and the food industry. This study aimed to examine artisanal cheeses for the presence of L. monocytogenes and the effectiveness of the MALDI-TOF MS method in the identification of the L. monocytogenes isolates. A total of 370 samples of artisanal cheeses were examined. L. monocytogenes was found in 23 cheese samples (6.2%). The reliability of L. monocytogenes identification achieved by MALDI-TOF MS was varied, and the vast majority of the isolates (27/32) were identified only to the secure genus, probable species level. This study showed that (i) the occurrence of L. monocytogenes in the artisanal cheeses was at a higher level than that in the other EU countries, (ii) the standard of species identification of L. monocytogenes isolates from artisanal cheeses achieved by MALDI-TOF MS was not satisfactory and (iii) the presence of L. monocytogenes in artisanal cheeses remains a problem with regard to the food safety criterion and a potential public health risk.

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Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry

The Open Microbiology Journal ◽

10.2174/1874285801307010118 ◽

2013 ◽

Vol 7 (1) ◽

pp. 118-122 ◽

Cited By ~ 15

Author(s):

Reiner Schaumann ◽

Nicolas Knoop ◽

Gelimer H Genzel ◽

Kevin Losensky ◽

Christiane Rosenkranz ◽

...

Keyword(s):

Mass Spectrometry ◽

Salmonella Enterica ◽

Reference Database ◽

Alternative Analysis ◽

Gram Negative ◽

Maldi Tof Ms ◽

E Coli ◽

Maldi Tof Mass Spectrometry ◽

Maldi Tof ◽

Tof Ms

Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has proven to be an effective identification tool in medical microbiology. Discrimination to subspecies or serovar level has been found to be challenging using commercially available identification software. By forming our own reference database and using alternative analysis methods, we could reliably identify all implemented Enterobacteriaceae and non-fermenting gram negative bacilli by MALDI-TOF MS and even succeeded to distinguish Shigella sonnei from Escherichia coli (E. coli) and Salmonella enterica spp. enterica serovar Enteritidis from Salmonella enterica spp. enterica serovar Typhimurium. Furthermore, the method showed the ability to separate Enterohemorrhagic E. coli (EHEC) and Enteropathogenic E. coli (EPEC) from non-enteropathogenic E. coli.

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Review of the impact of MALDI-TOF MS in public health and hospital hygiene, 2018

Eurosurveillance ◽

10.2807/1560-7917.es.2019.24.4.1800193 ◽

2019 ◽

Vol 24 (4) ◽

Cited By ~ 16

Author(s):

Belén Rodríguez-Sánchez ◽

Emilia Cercenado ◽

Alix T. Coste ◽

Gilbert Greub

Keyword(s):

Public Health ◽

Control Measures ◽

Infection Prevention And Control ◽

Clinical Samples ◽

Hospital Hygiene ◽

Bacterial Typing ◽

Maldi Tof Ms ◽

Maldi Tof ◽

The Impact ◽

Tof Ms

Introduction MALDI-TOF MS represents a new technological era for microbiology laboratories. Improved sample processing and expanded databases have facilitated rapid and direct identification of microorganisms from some clinical samples. Automated analysis of protein spectra from different microbial populations is emerging as a potential tool for epidemiological studies and is expected to impact public health. Aim To demonstrate how implementation of MALDI-TOF MS has changed the way microorganisms are identified, how its applications keep increasing and its impact on public health and hospital hygiene. Methods A review of the available literature in PubMED, published between 2009 and 2018, was carried out. Results Of 9,709 articles retrieved, 108 were included in the review. They show that rapid identification of a growing number of microorganisms using MALDI-TOF MS has allowed for optimisation of patient management through prompt initiation of directed antimicrobial treatment. The diagnosis of Gram-negative bacteraemia directly from blood culture pellets has positively impacted antibiotic streamlining, length of hospital stay and costs per patient. The flexibility of MALDI-TOF MS has encouraged new forms of use, such as detecting antibiotic resistance mechanisms (e.g. carbapenemases), which provides valuable information in a reduced turnaround time. MALDI-TOF MS has also been successfully applied to bacterial typing. Conclusions MALDI-TOF MS is a powerful method for protein analysis. The increase in speed of pathogen detection enables improvement of antimicrobial therapy, infection prevention and control measures leading to positive impact on public health. For antibiotic susceptibility testing and bacterial typing, it represents a rapid alternative to time-consuming conventional techniques.

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