Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material: in vitro and in vivo studies

2020 ◽  
Vol 29 (5) ◽  
pp. 270-280
Author(s):  
Majid Salehi ◽  
Saeed Farzamfar ◽  
Arian Ehterami ◽  
Zahrasadat Paknejad ◽  
Farshid Bastami ◽  
...  
Keyword(s):  
Polyvinyl Alcohol ◽  
Polymer Blend ◽  
Cellular Response ◽  
Blood Compatibility ◽  
Transmission Rate ◽  
Weight Ratio ◽  
In Vivo Studies ◽  
Collagen Fibres ◽  
Uptake Capacity ◽  
Sterile Gauze

Objective: To evaluate the application of a fabricated dressing containing kaolin for skin regeneration in a rat model of excisional wounds. Method: In the present study, kaolin was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing. To make the yarns, kaolin with weight ratio of 5% was added to PVA/chitosan polymer blend and subsequently formed into nanofibres using the electrospinning method. Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line. Rats with full-thickness excisional wounds were treated with kaolin-containing and kaolin-free dressings. Results: The study showed that rats treated with the kaolin-incorporated mats demonstrated a significant closure to nearly 97.62±4.81% after 14 days compared with PVA/chitosan and the sterile gauze, which showed 86.15±8.11% and 78.50±4.22% of wound closure, respectively. The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres. Conclusion: Our results show the potential applicability of PVA/chitosan/kaolin scaffolds as a wound care material.

scholarly journals Poly(Vinylamine) Derived N-Doped C-Dots with Antimicrobial and Antibiofilm Activities

2021 ◽  
Vol 7 (2) ◽  
pp. 40
Author(s):  
Semiha Duygu Sutekin ◽  
Mehtap Sahiner ◽  
Selin Sagbas Suner ◽  
Sahin Demirci ◽  
Olgun Güven ◽  
...  
Keyword(s):  
Blood Clotting ◽  
Minimum Bactericidal Concentration ◽  
Blood Compatibility ◽  
Weight Ratio ◽  
Gram Negative Bacteria ◽  
Biofilm Inhibition ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

Nitrogen-doped carbon dots (N-doped C-dots) was synthesized by using poly(vinyl amine) (PVAm) as a nitrogen source and citric acid (CA) as a carbon source via the hydrothermal method. Various weight ratios of CA and PVAm (CA:PVAm) were used to synthesize N-doped C-dots. The N-doped C-dots revealed emission at 440 nm with excitation at 360 nm and were found to increase the fluorescence intensity with an increase in the amount of PVAm. The blood compatibility studies revealed no significant hemolysis for N-doped C-dots that were prepared at different ratios of CA:PVAm for up to 500 μg/mL concentration with the hemolysis ratio of 1.96% and the minimum blood clotting index of 88.9%. N-doped C-dots were found to be more effective against Gram-positive bacteria than Gram-negative bacteria, with the highest potency on Bacillus subtilis (B. subtilis). The increase in the weight ratio of PVAm in feed during C-dots preparation from 1 to 3 leads to a decrease of the minimum bactericidal concentration (MBC) value from 6.25 to 0.75 mg/mL for B. subtilis. Antibiofilm ability of N-doped C-dots prepared by 1:3 ratio of CA:PVAm was found to reduce %biofilm inhibition and eradication- by more than half, at 0.78 mg/mL for E. coli and B. subtilis generated biofilms and almost destroyed at 25 mg/mL concentrations.

scholarly journals Gellan Gum Hydrogels Filled Edible Oil Microemulsion for Biomedical Materials: Phase Diagram, Mechanical Behavior, and In Vivo Studies

Polymers ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 3281
Author(s):  
Muhammad Zulhelmi Muktar ◽  
Muhammad Ameerul Amin Bakar ◽  
Khairul Anuar Mat Amin ◽  
Laili Che Rose ◽  
Wan Iryani Wan Ismail ◽  
...  
Keyword(s):  
Phase Diagram ◽  
Thermal Behavior ◽  
Chemical Interaction ◽  
Ternary Phase Diagram ◽  
Transmission Rate ◽  
Wound Care ◽  
Gellan Gum ◽  
In Vivo Studies ◽  
Histological Evaluation

The demand for wound care products, especially advanced and active wound care products is huge. In this study, gellan gum (GG) and virgin coconut oil (VCO) were utilized to develop microemulsion-based hydrogel for wound dressing materials. A ternary phase diagram was constructed to obtain an optimized ratio of VCO, water, and surfactant to produce VCO microemulsion. The VCO microemulsion was incorporated into gellan gum (GG) hydrogel (GVCO) and their chemical interaction, mechanical performance, physical properties, and thermal behavior were examined. The stress-at-break (σ) and Young’s modulus (YM) of GVCO hydrogel films were increased along with thermal behavior with the inclusion of VCO microemulsion. The swelling degree of GVCO hydrogel decreased as the VCO microemulsion increased and the water vapor transmission rate of GVCO hydrogels was comparable to commercial dressing in the range of 332–391 g m−2 d−1. The qualitative antibacterial activities do not show any inhibition against Gram-negative (Escherichia coli and Klebsiella pneumoniae) and Gram-positive (Staphylococcus aureus and Bacillus subtilis) bacteria. In vivo studies on Sprague–Dawley rats show the wound contraction of GVCO hydrogel is best (95 ± 2%) after the 14th day compared to a commercial dressing of Smith and Nephew Opsite post-op waterproof dressing, and this result is supported by the ultrasound images of wound skin and histological evaluation of the wound. The findings suggest that GVCO hydrogel has the potential to be developed as a biomedical material.

Bio-nanocomposite films based on cellulose nanocrystals filled polyvinyl alcohol/chitosan polymer blend

2015 ◽  
Vol 132 (22) ◽  
pp. n/a-n/a ◽  
Author(s):  
Nassima El Miri ◽  
Karima Abdelouahdi ◽  
Mohamed Zahouily ◽  
Aziz Fihri ◽  
Abdellatif Barakat ◽  
...  
Keyword(s):  
Polyvinyl Alcohol ◽  
Polymer Blend ◽  
Cellulose Nanocrystals ◽  
Nanocomposite Films

Efficiency and Stability Enhancement of Quasi-Solid-State Dye-Sensitized Solar Cells Based on PEO Composite Polymer Blend Electrolytes

2015 ◽  
Vol 1131 ◽  
pp. 186-192 ◽  
Author(s):  
Voranuch Somsongkul ◽  
Surassawatee Jamikorn ◽  
Atchana Wongchaisuwat ◽  
San H. Thang ◽  
Marisa Arunchaiya
Keyword(s):  
Polymer Blend ◽  
Electrochemical Impedance ◽  
Dye Sensitized Solar Cells ◽  
Weight Ratio ◽  
Composite Polymer Electrolyte ◽  
Composite Polymer ◽  
Sem Images ◽  
Cell Efficiency ◽  
Poly Ethylene ◽  
Polymer Blend Electrolyte

The composite polymer electrolyte consisting of poly (ethylene oxide) (PEO), KI, I2 and TiO2 was blended with low molecular weight poly (ethylene glycol) (PEG) and (PEG-MA)-Ru. The SEM images of these blended PEO electrolytes showed better dispersion of materials and the electrochemical impedance spectroscopic study showed an increase in conductivity compared to that of composite PEO electrolyte. These results were consistent with enhanced efficiency of DSSCs using these blended PEO electrolytes. The energy conversion efficiencies of DSSCs using composite PEO-PEG, PEO-(PEG-MA)-Ru and PEO-PEG-(PEG-MA)-Ru polymer blend electrolytes were 5.47, 5.05 and 5.28, respectively compared to 4.99 of DSSC using composite PEO electrolyte. The long-term storage of unsealed DSSCs at room temperature for 93 days demonstrated that the cell efficiency gradually decreased to 0.49-1.88%. DSSCs assembled with composite polymer blend electrolyte showed a slower decrease than that of DSSC using composite PEO electrolyte. It was found that the composite PEO-PEG-(PEG-MA)-Ru polymer blend electrolyte of 1.0:0.1:0.1 weight ratio gave the best improvement in stability of DSSCs.

Tailoring in vitro biological and mechanical properties of polyvinyl alcohol reinforced with threshold carbon nanotube concentration for improved cellular response

RSC Advances ◽  
2016 ◽  
Vol 6 (46) ◽  
pp. 39982-39992 ◽  
Author(s):  
Tejinder Kaur ◽  
Arunachalam Thirugnanam
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Polyvinyl Alcohol ◽  
Bone Tissue ◽  
Cellular Response ◽  
Natural Bone ◽  
Tissue Constructs ◽  
Living Bone

The development of living bone tissue constructs with structural, mechanical and functional similarities to natural bone are the major challenges in bone tissue engineering.

Erythropoietin/aloe vera-releasing wet-electrospun polyvinyl alcohol/chitosan sponge-like wound dressing: In vitro and in vivo studies

2017 ◽  
Vol 33 (3) ◽  
pp. 269-281 ◽  
Author(s):  
Mahdi Naseri-Nosar ◽  
Saeed Farzamfar ◽  
Majid Salehi ◽  
Ahmad Vaez ◽  
Roksana Tajerian ◽  
...  
Keyword(s):  
Polyvinyl Alcohol ◽  
Wound Dressing ◽  
Aloe Vera ◽  
In Vivo Studies
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