scholarly journals Occurrence of extended-spectrum beta-lactamase producing E. coli in broiler farm workers and the farm environment in Chiang Mai-Lamphun, Thailand

2020 ◽  
Vol 19 (1) ◽  
pp. 23-35
Author(s):  
Jutamart Rodroo ◽  
◽  
Montira Intanon ◽  
Khwanchai Kreausukon ◽  
Aphisek Kongkaew ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Antimicrobial Agents ◽  
Close Contact ◽  
Farm Workers ◽  
Beta Lactamase ◽  
Global Public Health ◽  
Chiang Mai ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum

Antimicrobial resistance has become a major global public health threat. Extended-spectrum beta-lactamase (ESBL) producing E. coli appears as an emergence cause of treatment failure and increase mortality due to limited available effective antimicrobial agents. This study was conducted to determine the occurrence and antimicrobial resistance of ESBL producing E. coli in broilers, farm workers and environment in broiler farms in Chiang Mai-Lamphun, Thailand. The prevalence of ESBL producing E. coli in the broiler farms was 60.4% (29/48). The prevalence of ESBL producing E. coli from boot swabs, farm worker’s rectal swabs, feed and water samples were 43.8%, 55.7%, 12.5% and 2.1%, respectively. All isolates showed susceptible to imipenem and, in contrast, resistant to ampicillin. The results demonstrated high antimicrobial resistant rate to streptomycin (94.3%), gentamicin (86.8%), tetracycline (77.4%), chloramphenicol (66.0%), nalidixic acid (58.5%), and sulfamethoxazole/trimethoprim (56.6%). High percentage (96.2%) of isolates was classified as multidrug resistance (MDR). Thirty-five antimicrobial resistance profiles were identified with AMP-GEN-SXT-NAL-TET-CHL-STR, AMP-GEN-SXT-TET-CHL-STR (14.3%) as the 2 most prevalent profiles. The common resistance profiles between farm workers and broilers was demonstrated. These findings are suggestive for possible transmission between poultry and humans in broiler farms, most likely via close contact. Antimicrobial usage should be strictly controlled together with increase awareness on hygiene practices in broiler farms.

scholarly journals Extended Spectrum Beta-Lactamase Escherichia coli in River Waters Collected from Two Cities in Ghana, 2018–2020

2021 ◽  
Vol 6 (2) ◽  
pp. 105
Author(s):  
Regina Ama Banu ◽  
Jorge Matheu Alvarez ◽  
Anthony J. Reid ◽  
Wendemagegn Enbiale ◽  
Appiah-Korang Labi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
River Waters ◽  
E Coli ◽  
Extended Spectrum ◽  
Two Cities ◽  
Animal Wastewater ◽  
Sources Of Contamination

Infections by Extended-Spectrum Beta-Lactamase producing Escherichia coli (ESBL-Ec) are on the increase in Ghana, but the level of environmental contamination with this organism, which may contribute to growing Antimicrobial Resistance (AMR), is unknown. Using the WHO OneHealth Tricycle Protocol, we investigated the contamination of E. coli (Ec) and ESBL-Ec in two rivers in Ghana (Odaw in Accra and Okurudu in Kasoa) that receive effluents from human and animal wastewater hotspots over a 12-month period. Concentrations of Ec, ESBL-Ec and percent ESBL-Ec/Ec were determined per 100 mL sample. Of 96 samples, 94 (98%) were positive for ESBL-Ec. concentrations per 100 mL (MCs100) of ESBL-Ec and %ESBL-Ec from both rivers were 4.2 × 104 (IQR, 3.1 × 103–2.3 × 105) and 2.79 (IQR, 0.96–6.03), respectively. MCs100 were significantly lower in upstream waters: 1.8 × 104 (IQR, 9.0 × 103–3.9 × 104) as compared to downstream waters: 1.9 × 106 (IQR, 3.7 × 105–5.4 × 106). Both human and animal wastewater effluents contributed to the increased contamination downstream. This study revealed high levels of ESBL-Ec in rivers flowing through two cities in Ghana. There is a need to manage the sources of contamination as they may contribute to the acquisition and spread of ESBL-Ec in humans and animals, thereby contributing to AMR.

scholarly journals Incidence and Antimicrobial Susceptibility of Escherichia coli and Klebsiella pneumoniae with Extended-Spectrum β-Lactamases in Community- and Hospital-Associated Intra-Abdominal Infections in Europe: Results of the 2008 Study for Monitoring Antimicrobial Resistance Trends (SMART)

2010 ◽  
Vol 54 (7) ◽  
pp. 3043-3046 ◽  
Author(s):  
Stephen P. Hawser ◽  
Samuel K. Bouchillon ◽  
Daryl J. Hoban ◽  
Robert E. Badal ◽  
Rafael Cantón ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Antimicrobial Susceptibility ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Abdominal Infections

ABSTRACT From 2002 to 2008, there was a significant increase in extended-spectrum beta-lactamase (ESBL)-positive Escherichia coli isolates in European intra-abdominal infections, from 4.3% in 2002 to 11.8% in 2008 (P < 0.001), but not for ESBL-positive Klebsiella pneumoniae isolates (16.4% to 17.9% [P > 0.05]). Hospital-associated isolates were more common than community-associated isolates, at 14.0% versus 6.5%, respectively, for E. coli (P < 0.001) and 20.9% versus 5.3%, respectively, for K. pneumoniae (P < 0.01). Carbapenems were consistently the most active drugs tested.

scholarly journals Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Meningitis and Cerebral Abscess in a Neonate: Therapeutic Challenge

2019 ◽  
Vol 2019 ◽  
pp. 1-5
Author(s):  
Surasak Puvabanditsin ◽  
Marianne Jacob ◽  
Maaz Jalil ◽  
Samhita Bhattarai ◽  
Qaiser Patel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Agents ◽  
Cerebral Abscess ◽  
Term Neonate ◽  
Resistant Bacteria ◽  
Beta Lactamase ◽  
Therapeutic Challenge ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum

We report a case of a 12-day-old term neonate with extended-spectrum beta-lactamase (ESBL) producing Escherichia coli (E. coli) meningitis and cerebral abscess. The patient received a 7-day course of antibiotics just few days prior to the infection. The incidence of infections from ESBL-producing E. coli is increasingly emerging. Antimicrobial agents must be vigilantly utilized to prevent the new highly resistant bacteria.

scholarly journals Characterization of Multidrug Resistant Extended-Spectrum Beta-Lactamase-ProducingEscherichia coliamong Uropathogens of Pediatrics in North of Iran

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Mohammad Sadegh Rezai ◽  
Ebrahim Salehifar ◽  
Alireza Rafiei ◽  
Taimour Langaee ◽  
Mohammadreza Rafati ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Multidrug Resistant ◽  
Beta Lactamase ◽  
Beta Lactam ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Beta Lactam Antibiotics ◽  
Extended Spectrum ◽  
Extended Spectrum Beta Lactamases ◽  
North Of Iran

Escherichia coliremains as one of the most important bacteria causing infections in pediatrics and producing extended-spectrum beta-lactamases (ESBLs) making them resistant to beta-lactam antibiotics. In this study we aimed to genotype ESBL-producingE. coliisolates from pediatric patients for ESBL genes and determine their association with antimicrobial resistance. One hundred of theE. coliisolates were initially considered ESBL producing based on their MIC results. These isolates were then tested by polymerase chain reaction (PCR) for the presence or absence ofCTX,TEM,SHV,GES, andVEBbeta-lactamase genes. About 30.5% of isolatedE. coliwas ESBL-producing strain. TheTEMgene was the most prevalent (49%) followed bySHV(44%),CTX(28%),VEB(8%), andGES(0%) genes. The ESBL-producingE. coliisolates were susceptible to carbapenems (66%) and amikacin (58%) and showed high resistance to cefixime (99%), colistin (82%), and ciprofloxacin (76%). In conclusion, carbapenems were the most effective antibiotics against ESBl-producingE. coliin urinary tract infection in North of Iran. The most prevalent gene is the TEM-type, but the other resistant genes and their antimicrobial resistance are on the rise.

scholarly journals Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli strains in dairy farm wastewater in Chiang Mai

2021 ◽  
Vol 19 (3) ◽  
pp. 349-362
Author(s):  
Prayuth Saekhow ◽  
◽  
Chayaphon Sriphannam ◽  
◽  
Keyword(s):  
Escherichia Coli ◽  
Dairy Farm ◽  
Pcr Amplification ◽  
Beta Lactamase ◽  
Chiang Mai ◽  
Phylogenetic Groups ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Farm Wastewater

We investigated the prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains in dairy farm wastewater in Chiang Mai, Thailand. We analyzed wastewater samples collected from 150 dairy farms and found that 88.7% of the farms (n = 133) were positive for ESBL-producing E. coli. Multiplex polymerase chain reaction (PCR) amplification was performed to characterize the presence of bla CTX-M, bla TEM, and blaSHV in ESBL-producing isolates. blaCTX-M was found in all isolates (n = 133), followed by blaTEM (80/133, 60.2%), whereas blaSHV was not detected in any isolate. blaCTX-M and blaTEM were present in 60.2% (80/133) of the isolates, and 39.8% (53/133) isolates carried bla CTX-M alone. Subgroup analysis showed that CTX-M-1 was the most prevalent subgroup among the isolates (129/133, 97.0%), followed by CTX-M-8 (2/133, 1.5%) and CTX-M-9 (2/133, 1.5%). The distribution of the phylogenetic groups was as follows: group A (100/133, 75.2%), followed by B1 (14/133, 10.5%), D (6/133, 4.5%), F (6/133, 4.5%), B2 (4/133, 3.0%), and E (3/133, 2.3%). Based on enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and dendrogram analysis, 24 isolates were classified into clades I (n = 21), II (n =1), and III (n =2). Minor genetic differences were found in all clade I isolates. Our data suggest that the circulating of ESBL-producing E. coli carried at least one bla gene strain distributed in dairy farm wastewater in Chiang Mai.

scholarly journals Antimicrobial Resistance Profile and Extended Spectrum Beta-Lactamase Resistance Genes in Escherichia coli from Poultry Droppings in Nasarawa, Nigeria

2021 ◽  
pp. 47-56
Author(s):  
S. C. Tama ◽  
Y. B. Ngwai ◽  
G. R. I. Pennap ◽  
I. H. Nkene ◽  
R. H. Abimiku ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Antimicrobial Resistance Profile ◽  
Poultry Farms ◽  
Poultry Droppings

Aims: This study investigated the antimicrobial resistance profile and extended spectrum beta-lactamase resistance genes of Escherichia coli isolated from droppings of from selected poultry farms in Nasarawa, Nigeria. Study Design: Investigative Place and Duration of Study: Department of Microbiology, Nasarawa State University, Keffi, between November 2019 and February 2020. Methodology: A total of 90 samples from poultry droppings were collected from selected farms. Escherichia coli was isolated from the samples using standard cultural and microbiological methods. Antibiotic susceptibility testing and minimum inhibitory concentrations were evaluated as described by the Clinical and Laboratory Standards Institute (CLSI). The detection of extended-spectrum beta-lactamase (ESBL) production in E. coli isolates was carried out using double disc synergy test.  In addition, molecular detection of ESBL genes was carried out using Polymerase Chain Reaction (PCR) method. Results: The prevalence of E. coli was 100%. Antibiotic resistances of E. coli were recorded as follows: streptomycin (S: 94.4%), sulphamethoxazole / trimethoprim (SXT: 90.0%), ampicillin (AMP: 88.9%), gentamicin (CN: 68.9%), amoxicillin/clavulanic acid (AMC: 55.6%), ciprofloxacin (CIP: 41.1%), cefoxitin (FOX: 35.6%), ceftazidime (CAZ: 34.4%), cefotaxime (CTX: 22.2%), and imipenems (IPM: 17.8%). The most common antibiotic resistant resistance phenotype was AMP-CTX-CAZ-CIP-CN (11.1%). Multiple antibiotic resistance (MAR) was observed in 97.7% (88/90) of the isolates, with the common MAR index being 0.5 (33.3%). Twenty five of the thirty beta-lactam resistant isolates (83.3%) were confirmed ESBL producers. The 25 ESBL positive isolates carried bla genes as follows: blaTEM (11/25, 44.0%) and blaCTX-M (18/25, 72.0%). blaSHV was not found in any isolate. Conclusion: E. coli isolated from the droppings of selected poultry farms in Nasarawa were less resistant to imipenem, cefotaxime, ceftazidime and cefoxitin in the study location. This implies that the antibiotics are useful in the treatment of infection caused by E. coli. Also, ESBL-positive E. coli isolates harbored ESBL genes, with blaCTX-M as the most common.

scholarly journals Antimicrobial resistance characteristics of Extended Spectrum Beta Lactamase (ESBL)- producing Escherichia coli from dairy farms in the Sleman district of Yogyakarta province, Indonesia

2021 ◽  
Vol 19 (3) ◽  
pp. 525-535
Author(s):  
Kusnul Yuli Maulana ◽  
◽  
Duangporn Pichpol ◽  
Nur Rohmi Farhani ◽  
Dyah Ayu Widiasih ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Antimicrobial Resistance ◽  
Dairy Farms ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Microbiological Methods ◽  
Water Feed

Extended Spectrum Beta Lactamase (ESBL)-producing Escherichia coli (E. coli) infections are a global health challenge resulting from human contact with infected animals and contaminated farm environments. This study aims to identify antimicrobial resistance patterns of ESBL-producing E. coli isolated from dairy farms in the Sleman District of Yogyakarta Province, Indonesia. Ninety-three dairy farms with a history of antibiotic use in the previous 6 months were identified. Samples were collected from 6 different sources (feces, milk, wastewater, animal drinking water, feed and rinses of workers’ hands) on each farm during August through November 2020. These samples were cultured with conventional microbiological methods for the isolation of ESBL-producing E. coli. ESBL-producing E. coli was identified in one or more of the sources in 54% (50/93) of the dairy farms sampled. Fecal samples were the most commonly positive (25%) while wastewater, animal drinking water feed, milk and hand rinses were positive at 16%, 10%, 5%, 4% and 3% respectively. Colonies from each positive sample were screened for antibiotic susceptibility test using the Vitek-2 system. Resistance to trimethoprim/sulfamethoxazole, tetracycline and gentamicin were found in 74%, 63% and 48% of the isolates, respectively. Multidrug resistant (MDR) was identified in 50% (63/127) of the isolates. In conclusion, ESBL-producing E. coli appears widespread in dairy farms using antibiotics and antimicrobial resistance among these bacteria is common in this study area. Further study of the risk of human transmission from contaminated cattle and their environments could benefit the national antimicrobial resistance strategic plans.

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