scholarly journals Studies on Variation in Arecoline Content of Arecanuts Collected from Different Parts of Karnataka

2017 ◽  
Vol 14 (2) ◽  
pp. 691-695
Author(s):  
B. R. Gurumurthy ◽  
H. C. Swathi ◽  
J. Sahana ◽  
S. P. Nataraj
Keyword(s):  
Environmental Factors ◽  
Extraction Method ◽  
Hplc Method ◽  
Areca Nut ◽  
Differential Processing ◽  
Specificity And Sensitivity ◽  
Areca Catechu ◽  
Different Parts ◽  
Oily Liquid

ABSTRACT: Arecoline is a nicotinic acid-based alkaloid found in the areca nut, the fruit of the areca palm (Areca catechu). It is an oily liquid that is soluble in water, alcohols, and ether. HPLC method is simple and rapid for determination of arecoline content in areca nut. Areca samples were collected from Shimoga, Davanagere, Chikkamagalur, Chitradurga, Dakshina kannada and Udupi districts of Karnataka, India. The collected areca samples were powdered and arecoline is extracted from samples collected from different hoblies of the districts. The extraction method was optimized to obtain pure arecoline before analysis to separate any interference in order to maximize the specificity and sensitivity of the method. The regionwise arecoline content has been compared. The concentration of arecoline varied from area to area depending on environmental factors, differential processing methods, age of the plantations, varietal differences etc.

scholarly journals Stability Indicating RP-HPLC Method for the Simultaneous Determination of Atorvastatin Calcium, Metformin Hydrochloride, and Glimepiride in Bulk and Combined Tablet Dosage Form

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Devi Ramesh ◽  
Mohammad Habibuddin
Keyword(s):  
Dosage Form ◽  
Degradation Products ◽  
Hplc Method ◽  
Metformin Hydrochloride ◽  
Atorvastatin Calcium ◽  
Rp Hplc ◽  
Specificity And Sensitivity ◽  
Validation Parameters ◽  
Tablet Dosage

A simple, rapid, and precise RP-HPLC method for simultaneous analysis of atorvastatin calcium, metformin hydrochloride, and glimepiride in bulk and its pharmaceutical formulations has been developed and validated. These drugs were separated by using Grace Smart Altima C-8 column (250 × 4.6 mm, 5-μm) with a mobile phase consisting of acetonitrile : phosphate buffer (60 : 40 (v/v), pH 3.0) at a flow rate of 1 mL/min, injection volume 25 µL, and detection at 235 nm. Metformin, atorvastatin, and glimepiride were eluted with retention times of 2.57 min, 7.06 min, and 9.39 min, respectively. The method was validated for accuracy, precision, linearity, specificity, and sensitivity in accordance with ICH (Q2B) guidelines. The results of all the validation parameters were found to be within the acceptable limits. The calibration plots were linear over the concentration ranges from 10 to 150 µg/mL, 20 to 200 µg/mL, and 10 to 150 µg/mL for atorvastatin, metformin, and glimepiride, respectively. The accuracy and precision were found to be between 98.2%–105% and ≤2% for three drugs. Developed method was successfully applied for the determination of the drugs in tablet dosage form and recovery was found to be >98% for three drugs. The degradation products produced as a result of stress studies did not interfere with drug peaks.

scholarly journals PRITHVI MAHABHUTA DOMINANT CHARACTERS IN PUGAPHALA (ARECA CATECHU LINN.) SCIENTIFICALLY ASSESSED THROUGH PHARMACOGNOSY AND PHARMACEUTICAL CHEMISTRY

2018 ◽  
Vol 10 (4) ◽  
pp. 75
Author(s):  
Kamla Moond ◽  
Hitesh Vyas ◽  
Harisha C. R. ◽  
V. J. Shukla
Keyword(s):  
Chemical Analysis ◽  
Pharmaceutical Chemistry ◽  
Areca Nut ◽  
Betel Nut ◽  
Tropical Fruit ◽  
Areca Catechu ◽  
The World ◽  
Powdered Form ◽  
Different Parts

Objective: The Areca catechu L. is a tropical fruit, which is also called betel nut and is widely distributed in different parts of the world. Areca catechu L is used for various treatment aliments in the form of various preparations especially in powdered form and it used extensively in Ayurveda to treat Mukhavikara, Aruchi, Yonishaithilya, Shvetapradara etc. Areca nut is commonly used as betel nut or supari, as it is often chewed wrapped in betel leaves (Paan). The aim is to assessment of Mahabhautika dominance in Pugaphala by pharmacognostical and pharmaceutical study.Methods: Microscopic, macroscopic study and phsico-chemical analysis of Pugaphala Churna.Results: In present study Pugaphala was selected as a Parthiva dominant drug according to its Rasa Panchaka, after that its Mahabhautika dominancy was assessed by pharmacognostical and pharmaceutical study and results also support that in Pharmaceutical study loss on drying is 4.4%w/w, in Pharmacognostical study Rhomboidal crystal, Lignified scleroid etc. were found these characters also showed that Pugaphala is having dominancy of Prithvi Mahabhuta.Conclusion: Prithvi dominancy in Pugaphala is scientifically assessed by Pharmacognostical and Pharmaceutical study.

scholarly journals Development of a HPLC Method for the Quantitative Determination of Capsaicin in Collagen Sponge

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Chun-Lian Guo ◽  
Hong-Ying Chen ◽  
Bi-Ling Cui ◽  
Yu-Huan Chen ◽  
Yan-Fang Zhou ◽  
...  
Keyword(s):  
Ultrasonic Wave ◽  
Extraction Method ◽  
Chromatographic Method ◽  
Pharmaceutical Products ◽  
Hplc Method ◽  
Collagen Sponge ◽  
Hplc Assay ◽  
The Mean ◽  
Perfect Recovery

Controlling the concentration of drugs in pharmaceutical products is essential to patient’s safety. In this study, a simple and sensitive HPLC method is developed to quantitatively analyze capsaicin in collagen sponge. The capsaicin from sponge was extracted for 30 min with ultrasonic wave extraction technique and methanol was used as solvent. The chromatographic method was performed by using isocratic system composed of acetonitrile-water (70 : 30) with a flow rate of 1 mL/min and the detection wavelength was at 280 nm. Capsaicin can be successfully separated with good linearity (the regression equation isA= 9.7182C+ 0.8547;R2= 1.0) and perfect recovery (99.72%). The mean capsaicin concentration in collagen sponge was 49.32 mg/g (RSD = 1.30%;n= 3). In conclusion, the ultrasonic wave extraction method is simple and the extracting efficiency is high. The HPLC assay has excellent sensitivity and specificity and is a convenient method for capsaicin detection in collagen sponge. This paper firstly discusses the quantitative analysis of capsaicin in collagen sponge.

Establishment and Evaluation of Determination of Cinnamaldehyde in Baoyuanqingxue Granules by HPLC

2014 ◽  
Vol 998-999 ◽  
pp. 372-377 ◽  
Author(s):  
Qiang Wu ◽  
Chang Hong Wang ◽  
Pu Wang ◽  
Xiang Rong Liu
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Extraction Method ◽  
Mobile Phase ◽  
High Performance ◽  
Hplc Method ◽  
Column Temperature ◽  
Average Recovery

To examine the extraction method and chromatographic conditions that affect the determination of cinnamaldehyde in Baoyuanqingxue granules and make clinical evaluation about the determination of cinnamaldehyde.Ultrasonic methanol extraction was used before the detemination of cinnamaldehyde in Baoyuanqingxue granules. High Performance Liquid chromatography (HPLC) method was applied to detect samples. The SB-C18 column (Agilent, ZORBAX, 4.6×150mm, 5μm) was adopted, the mobile phase was acetonitrile-water (35:65) at the flow rate of 1.00mL•min-1 with DAD detection wavelength at 290nm, the volume of injection was 20μL and the column temperature was 30°C. The resolution between cinnamaldehyde and other peaks was good. The calibration curve was linear in the range of 0.5035~50.35μg•mL-1(r=0.99976). The average recovery (n=6) of cinnamaldehyde was 99.2% with RSD of 0.5%. The HPLC-DAD method to detect the content of cinnamaldehyde in Baoyuanqingxue granules is simple and accurate. It can be used for quality control of cinnamaldehyde in Baoyuanqingxue granules.

scholarly journals A New Solid-Phase Extraction Method for Determination of Pantoprazole in Human Plasma Using High-Performance Liquid Chromatography

2019 ◽  
Vol 7 (11) ◽  
pp. 1757-1761 ◽  
Author(s):  
Dragica Zendelovska ◽  
Emilija Atanasovska ◽  
Kalina Gjorgjievska ◽  
Kristina Pavlovska ◽  
Krume Jakjovski ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Human Plasma ◽  
Extraction Method ◽  
High Performance ◽  
Solid Phase ◽  
Hplc Method ◽  
Phase Extraction ◽  
Good Precision ◽  
Plasma Samples

BACKGROUND: A new simple, selective and accurate high-performance liquid chromatographic (HPLC) method utilising solid-phase extraction for the determination of pantoprazole in human plasma samples has been developed. AIM: The purpose of this paper was developing a new HPLC method suitable for the determination of pantoprazole in plasma samples, which enables simple and rapid isolation and concentration of the analysed drug.METHODS: The chromatographic separation was accomplished on a LiChroCart LiChrospher 60 RP select B column using a mobile phase composed of 0.2 % (V/V) water solution of triethylamine (pH 7) and acetonitrile (58:42, V/V) followed by UV detection was at 280 nm. The solid-phase extraction method using LiChrolut RP-18 (200 mg, 3 ml) was applied to the obtained good separation of investigated drug from endogenous plasma components. Best results were achieved when plasma samples were buffered with 0.1 mol/L KH2PO4 (pH 9) before extraction, eluted and reconstituted with acetonitrile and 0.001 mol/L NaOH after extraction, respectively. RESULTS: The standard calibration curves showed good linearity within the range of 25.0-4000.0 ng/mL with a correlation coefficient greater than 0.996. Retention times of pantoprazole and internal standard, lansoprazole was 4.1 and 6.0 min respectively. The limit of quantification was 25.0 ng/mL. For intra- and inter-day precision relative standard deviations ranged from 4.2 to 9.3%. The relative errors for stability investigations were ranged from 0.12 to -10.5%. CONCLUSION: This method has good precision and accuracy and was successfully applied to the pharmacokinetic and bioequivalence study of 40 mg pantoprazole in healthy volunteers.

scholarly journals A VALIDATED ANALYTICAL HPLC METHOD FOR THE QUANTIFICATION OF LINCOMYCIN HYDROCHLORIDE IN BULK AND SOLID DOSAGE FORM

2017 ◽  
Vol 9 (3) ◽  
pp. 42
Author(s):  
P. Rajeev Kumar ◽  
Rekha Rajeevkumar
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Solid Dosage ◽  
Rp Hplc ◽  
Specificity And Sensitivity ◽  
Ich Guidelines ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Objective: Develop a simple isocratic reverse phase high performance liquid chromatographic method (RP-HPLC) and validate for the determination of lincomycin hydrochloride (LMH) in bulk and pharmaceutical preparations.Methods: RP-HPLC quantification was carried out by using fine pack SIL RPC18 column. The mobile phase (methanol: water) was pumped at a flow rate of 1 ml/min in the ratio of 90:10 v/v and the eluents were monitored at 254 nm.Results: The retention time of the drug was 3.73 min and produced at a linear response in the concentration range of 5-25µg/ml. The percentage RSD was found to be below 2%. The LOD and LOQ were found to be 0.854µg/ml and 0.258µg/ml respectively.Conclusion: Validation of the method was performed for precision, accuracy, linearity, ruggedness, specificity and sensitivity to conform to ICH guidelines for valuation for analytical methods.

Reverse-phase HPLC method for the simultaneous analysis of triclosan and triclocarban in surface waters

2010 ◽  
Vol 10 (2) ◽  
pp. 173-180 ◽  
Author(s):  
Irena Baranowska ◽  
Sylwia Magiera ◽  
Katarzyna Bortniczuk
Keyword(s):  
Simultaneous Determination ◽  
Surface Waters ◽  
Extraction Method ◽  
Mobile Phase ◽  
Solid Phase ◽  
Hplc Method ◽  
Isocratic Elution ◽  
Reverse Phase Hplc ◽  
Reverse Phase

A validated reverse-phase HPLC-DAD for the simultaneous determination of triclosan and triclocarban in surface water has been developed, because this method has not been used for determination of these disinfectant agents so far. An isocratic elution was achieved using a Develosil RP Aqueous AR-5 RP-30 column with a flow rate of 1.0 mL min−1. The mobile phase consisted of mixed methanol and water in raito of 90:10, v/v. DAD detector was used to monitor the analytes at 280 nm for triclosan and 265 nm for triclocarban. The time of analysis was 10 min and the retention time for triclosan and triclocarban was 5.81 min and 8.13 min, respectively. The solid phase extraction method was proposed for the preconcentration step. The extraction efficiencies were approximately 97% for triclosan and 87% for triclocarban. The linearity range for triclosan and triclocarban after pre-concentration were between 0.5–20 μg mL−1 and 0.3–20 μg mL−1, respectively. The LOD and LOQ of triclosan and triclocarban in real samples were 0.04 ng mL−1 and 0.11 ng mL−1, 0.17 ng mL−1 and 0.50 ng mL−1, respectively. The method has been sensitive and can be successfully applied to the fast and simultaneous determination of triclosan and triclocarban in surface waters.

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