scholarly journals MORTALITAS DAN RESORPSI FETUS MENCIT (Mus musculus L.) SETELAH PEMBERIAN EKSTRAK ETANOL TANAMAN SURUHAN (Peperomia pellucida (L.,) Kunth.)

2021 ◽  
pp. 194-202
Author(s):  
Hendri Busman ◽  
Nuning Nurcahyani ◽  
Yosi Dwi Saputra ◽  
Salman Farisi ◽  
Qotrunnada Salsabila
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Mus Musculus ◽  
Ethanol Extract ◽  
Control Group ◽  
P Value ◽  
Treatment Groups ◽  
Medicinal Use ◽  
One Way Anova ◽  
Different Doses

Tanaman Suruhan (Peperomia pellucida (L.,) Kunth.) merupakan tanaman obat yang memiliki senyawa metabolit sekunder seperti alkaloid, flavonoid, saponin, tanin, dan steroid. Berbagai senyawa kimia tersebut yang berpotensi sebagai obat tetapi perlu diperhatikan kemungkinan adanya efek samping terhadap organisme khususnya pada masa kehamilan. Penelitian ini bertujuan menguji efek teratogenik ekstrak tanaman suruhan terhadap fetus mencit (Mus musculus L.), meliputi mortalitas dan resorpsi. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan dengan  20 ekor mencit betina dibagi ke dalam 4 kelompok yaitu: K(+) (Aquabides), P1 diberi ekstrak tanaman suruhan dengan dosis (1,68 mg/g bb), P2 (3,36 mg/g bb), dan P3 (6,72 mg/g bb). Hasil penelitian terhadap persentase fetus yang mengalami mortalitas dan resorpsi fetus antara kontrol K(+) dan perlakuan dengan ekstrak etanol suruhan (P1, P2, dan P3) tidak menunjukkan perbedaan yang bermakna secara statistik berdasarkan uji ANOVA satu faktor (p value 0,418). Hasil penelitian yang didapat menunjukkan bahwa ekstrak etanol tanaman suruhan tidak menyebabkan mortalitas pada fetus mencit, namun menyebabkan resorpsi pada fetus mencit pada pemberian dosis sebesar 1,62 mg/g BB, 3,36 mg/g BB, dan 6,72 mg/g BB.   Pepper elder (Peperomia pellucida (L.,) Kunth.) is a medicinal plant that has secondary metabolites such as alkaloids, flavonoids, saponins, tannins, and steroids. The side effects for organisms of those chemical compounds, which are potentially beneficial for their medicinal use, still need to be considered especially in pregnancy. This study aims to determine the teratogenic effects of pepper elder extract on mortality and resorption of mice (Mus musculus L.) fetus. This study uses total random sampling design with 4 treatments consisting of 20 female mice divided into 4 groups: the control group (K(+)) which is given Aqua distillation and the treatment groups which are all given the pepper elder extract with different doses, where the first treatment group (P1) is given 1.68 mg/g body weight of the extract, the second treatment group (P2) is given 3.36 mg/g body weight of the extract, and the third treatment group (P3) is given 6.72 mg/g body weight of the extract. The results obtained of mortality and resorption percentage of mice fetus between the control group (K(+)) and the treatment groups (P1, P2, and P3) showed that there is no statistically meaningful difference based on one-way ANOVA test (p value 0,418). This study showed that ethanol extract of pepper elder causes no mortality in mice fetus, yet it causes resorption on mice fetus at given doses of 1,62 mg/g body weight, 3,36 mg/g body weight, dan 6,72 mg/g body weight.

UJI AKTIVITAS ANALGETIK EKSTRAK ETANOL HERBA SEMBUKAN (Paederia foetida L.) PADA MENCIT PUTIH JANTAN (Mus musculus) YANG DIINDUKSI DENGAN ASAM ASETAT

2020 ◽  
Vol 5 (2) ◽  
pp. 358-363
Author(s):  
Triswanto Sentat ◽  
◽  
Fitri Handayani ◽  
Ellen Indraswari
Keyword(s):  
Body Weight ◽  
Analgesic Activity ◽  
Mus Musculus ◽  
Ethanol Extract ◽  
Optimal Dose ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Treatment Groups ◽  
Paederia Foetida

Sembukan plants (Paederia foetida L.) are wild vines that are usually used by people as potential medicinal plants for pain. The purpose of this study was to determine the ethanol extract of sembukan’s herbal analgesic activity in male white mice (Mus musculus) and determine the optimal dose of the ethanol extract from sembukan’s herbal (Paederia foetida L.) which has the potential as an analgesic. Sembukan’s herbal is extracted with 70% ethanol solvent and an analgesic activity test is divided into 5 treatment groups namely positive control group (potassium diclofenac), negative control, dose I (80 mg / kg body weight), dose II (160 mg / kg body weight) ) and dose III (320 mg / kg body weight) by oral administration. Thirty minutes after administration, the mice were given an indicator of pain, 0.5% acetic acid. Analgesic power is calculated by counting the amount of stretching of mice for 1 hour. From the results of the study, the ethanol extract of sembukan’s herbal has analgesic activity in male white mice with percent dose analgesic power 35.18%, dose II 53.58% and dose III 68.98%. The optimal dose that has potential as an analgesic in male white mice is dose III with 68.98% analgesic power.

scholarly journals The anticancer activity of ethanol extract of Chromolaena odorata leaves in 7,12-Dimethylbenz[a]anthracene in (DMBA) induced breast cancer Wistar rats (Rattus novergicus)

Pharmacia ◽  
2021 ◽  
Vol 68 (2) ◽  
pp. 493-499
Author(s):  
Hanifah Yusuf ◽  
Reno Keumalazia Kamarlis ◽  
Yusni Yusni ◽  
Marhami Fahriani
Keyword(s):  
Breast Cancer ◽  
Body Weight ◽  
Treatment Group ◽  
Anticancer Activity ◽  
Cancer Control ◽  
Ethanol Extract ◽  
Breast Cancer Chemotherapy ◽  
Treatment Groups ◽  
Post Hoc ◽  
Dose Dependent

Background: Breast cancer chemotherapy with standard drugs such as doxorubicin will induce cardiotoxicity. Therefore, this study aims to evaluate the anticancer activity of C. odorata leaves extract in DMBA induced breast cancer on rats. Methods: Seven groups of Rattus novergicus were used: Four treatment groups of C. odorata extract (500, 1000, 2000, and 4000 mg/kg BW), normal control, breast cancer control, and doxorubicin treatment group. The number, volume, and weight of the nodule and the rats’ body weight were compared among groups. Data was analyzed using paired t-test or one-way ANOVA with post hoc analysis as appropriate. Results: Significant decline of the number, volume, and weight of cancer nodules was observed in the treatment group (p < 0.001). The weight of the cancer nodule at week 16th was also significantly reduced in GCo2000 compared to Gdoxo (p < 0.0001). A significant increase in body weight was also dose-dependent, especially at week 11th (p < 0.05 in all comparisons) and week 16th (p < 0.001 in all comparisons). Conclusion: This study suggested that the ethanol extract of C. odorata leaves has anticancer and antiproliferative activity.

scholarly journals Antihyperglycemic Effectiveness Test of 96% Ethanol Extract of Soursop Leaves on Mus musculus Induced by Streptozotocin

2020 ◽  
Vol 52 (3) ◽  
pp. 131-138
Author(s):  
Nur Fadhillah Khalid ◽  
Peter Kabo ◽  
Natsir Djide
Keyword(s):  
Blood Glucose ◽  
Mus Musculus ◽  
Insulin Aspart ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Positive Control ◽  
Control Group ◽  
P Value ◽  
Annona Muricata ◽  
Cross Sectional

Annona muricata L. is widely known throughout Indonesia as having great potentials as an antidiabetic agent. This study aimed to evaluate the antihyperglycemic effectiveness of ethanol extract 96% from soursop leaves and to compare its effects to metformin and insulin aspart injection as antidiabetic agents. This was a cross-sectional experimental study with random sampling approach which was performed at Phytochemistry and Biopharmacy Laboratory, Faculty of Pharmacy, Hasannudin University, Indonesia, from February 17 to March 3, 2020. Twenty-four Mus musculus subjects induced by streptozotocin were divided into six groups, which received 96% ethanol extract from Annona muricata in various doses. The extract was produced using the maceration process. The fasting blood sugar level was measured using the point of care testing (POCT) method, and data collected were then analyzed using a univariate approach. Results showed that the average fasting blood glucose level (FBG) was decreasing with increasing doses (5 g/kgBW=127 mg/dL, 10 g/kgBW =114 mg/dL, and 15 g/kg BW=97.75 mg/dl with a p-value of 0.000 based on repeated ANOVA. When compared to the positive control 1 (metformin), the decrease in FBG level in this control group (70.67 mg/dL) was better than in the group that received ethanol extract from Annona muricata L. In subjects in positive control 2 (insulin aspart), the average FBG level remained high, which was 473 mg/dL. Hence, 96% ethanol extract of Annona muricata L. can effectively lower fasting blood glucose in Mus musculus.

scholarly journals Perubahan Kualitas Sperma Mencit (Mus musculus) Yang Terpapar Asap Rokok Elektrik

2019 ◽  
Vol 3 (2) ◽  
pp. 122-128
Author(s):  
Andita Ayu Mandasari ◽  
Siti Nur Asiyah ◽  
Kurnia Lintang
Keyword(s):  
Treatment Group ◽  
Cigarette Smoke ◽  
Mus Musculus ◽  
Group Treatment ◽  
Time Lag ◽  
Cigarette Smoke Exposure ◽  
Control Group ◽  
Male Mice ◽  
Suction Device ◽  
Treatment Groups

Smoking is one of the habits that can affect human health. One type of cigarette that is currently the choice is electric cigarettes. The e-cigarette content includes nicotine, propylene glycol, flavorings and glycerin. The purpose of this study was to determine the effect of exposure to e-cigarette smoke on mice strain balb / c with the parameters of the number of spermatozoa. This study used 15 male mice which were 8-10 weeks old and weighed 25-30 grams of male mice which were divided into 3 treatment groups namely control group, treatment group one and treatment group two. In the control group no exposure to cigarette smoke was carried out while in the treatment group one was exposed to cigarette smoke in mice with a dose of 0 mg nicotine and the second treatment group was exposed to e-cigarette smoke at a dose of 18 mg nicotine. Electric cigarette smoke exposure is carried out for 30 days using a suction device from a syringe. The exposure of cigarette smoke is carried out every day with successive doses of 20 times as much as 3 times with a time lag of 10 minutes (5 minutes the chamber is closed and 5 minutes of the chamber is opened). The results showed that the average number of spermatozoa in the control group was 16.816 million / ml. The average number of spermatozoa in treatment one was 10.432 million / ml and the average number of spermatozoa in treatment two was 5.234 million / ml. This shows that exposure to cigarette smoke can reduce the number of spermatozoa from the control group to the first treatment by 42.56% and the second treatment shows a decrease from the first group by 34.65%.

scholarly journals Antidepresant effects of Cinnamon (Cinnamomum burmannii) extract in depressed induced rats using 3-minutes Tail Suspension method

2019 ◽  
Vol 3 (3) ◽  
pp. 1-8
Author(s):  
Nita Parisa ◽  
Mayasari Mayasari ◽  
Nia Savitri Tamzil ◽  
Bintang Arroyantri ◽  
Ziske Maritska
Keyword(s):  
Animal Model ◽  
Positive Control ◽  
Control Group ◽  
P Value ◽  
Tail Suspension ◽  
Treatment Groups ◽  
Cinnamon Extract ◽  
Immobility Time ◽  
Cinnamomum Burmannii ◽  
One Way Anova

Abstract Background. The increasing prevalence of depression gives rise to challenges in not only elucidating its diverse causes, but also in finding an effective treatment. One of the factors linked to depression is the imbalance of serotonin, norepinephrine, and dopamine neurotransmitters.  Cinnamon (Cinnamomum burmannii) as one of the world’s wellknown cooking ingredients is believed to be able to regulate the neurotransmitters imbalance with the help of terpenoids and flavonoid polyphenols as one of its content. Objective. This study aims to determine the effectiveness of cinnamon extract as an antidepressant in depressed induced animal model. Methods. An experimental in vivo with pre-post control group design was conducted in twenty five Wistar strain white rats that were divided into 5 treatment groups that received fluoxetine as positive control, aquades, and different dose of cinnamon extracts (50 mg/kgBW, 100 mg/kgBW, and 200 mg/kgBW).  Depression induction method used was 3-minute Tail Suspension Test, done for 14 days. The antidepressant effectiveness test was carried out by calculating the immobility time duration with Forced Swimming Test method and was further analyzed using one-way ANOVA test. Results. One-way ANOVA test results showed that there were differences in the mean duration of immobility time between treatment groups after being given cinnamon extract (p value = 0,000). Groups that were given 100 mg/kgBW cinnamon extract and 200 mg /kgBW showed a p value>0.05 when compared with positive control group receiving Fluoxetine although displayed a similar reduced immobility time. Conclusion. Cinnamon (Cinnamomum burmannii) extract showed a promising potential as an effective antidepressant tested in animal model.     Keywords: cinnamon, extract, depression, immobility time, rat

scholarly journals Gambaran Histopatologi Paru Mencit (Mus musculus) Setelah Pemaparan Paraquat

2018 ◽  
Vol 11 (2) ◽  
pp. 7
Author(s):  
Aulia Wiradi ◽  
Wiwit Ade Fidiawati ◽  
Sri Melati Munir
Keyword(s):  
Experimental Study ◽  
Treatment Group ◽  
Mus Musculus ◽  
Lung Fibrosis ◽  
Control Group ◽  
Post Test ◽  
Lung Histopathology ◽  
Post Hoc ◽  
One Way Anova

Paraquat destroys organs including lung. Purpose of this study was to determine the effect of paraquat on histopathologyof lung, exspecially fibrosis in mice (Mus musculus). This study was an experimental study with post-test only withcontrol design and using 15 mice as sample. Samples were divided into 5 groups consist of one control group and fourdifferent doses of paraquat treatment group (25 mg / kg / day, 50 mg / kg / day, 75 mg / kg / day and 100 mg / kg / day for7 days with spraying). Analysis of the data have used One-Way ANOVA then post-hoc test with tukey to assesseddifferences of each groups. The results showed increasing of lung fibrosis with increasing doses and have significantcorrelations (p = 0.000). In conclusion, there are significant changes in lung histopathology of mice (Mus musculus)after paraquat exposure for 7 days.

The Effect of Moringa Oleifera Extract on Sperm Quality and Quantity of Diabetes Wistar Rat

2021 ◽  
Vol 2 (1) ◽  
pp. 91-96
Author(s):  
Novita Dwi Febrianty ◽  
Herisa Putri Anggraini ◽  
Meldawati ◽  
Boyke Marthin Simbolon
Keyword(s):  
Moringa Oleifera ◽  
Sperm Quality ◽  
Ethanol Extract ◽  
Wistar Rat ◽  
Control Group ◽  
P Value ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Chronic Disorder ◽  
Moringa Oleifera Leaves

A B S T R A C TDiabetes mellitus is a condition in which blood glucose levels increase in aperson's body which can result in chronic disorder. The effect of diabetes on amale reproductive system can affect sperm quality and disrupt the spermatogenesisprocess. Moringa oleifera leaves are rich in antioxidants such as flavonoids, vitaminA, vitamin E, and vitamin C, and contain selenium which helps lower bloodssugarlevels. This study was conductedtto determine the effect of administering Moringaoleifera leaf extract on the quality andiquantity ofssperms in Wistar rats with diabetesmellitus. This research is an experimental studyiusing pre-andipost-randomized,controlled group design. This study examinediblood sugarllevels, density,motility, andiviability ofssperm cells. This experimental test was dividediinto 4groups: one as a control group andtthree groups as treatment groups inducedwith alloxan and given ethanol extract of Moringa leaves with doses of 250, 500,and,750mg/kgBB. The results showed that the ethanol extract of Moringa leavesshowedssignificant improvements in various doses (P-value ≤ 0.05).

scholarly journals Ethanol Extract of Young Papaya Seeds (Carica Papaya, L ) Lower the Level of testosterone, Spermatozoa Count and Expression of Androgen Receptors in Sertoli Cells of Adult Mice (Mus Musculus)

2021 ◽  
Vol 14 (1) ◽  
pp. 207-213
Author(s):  
I Dewa Made Ruspawan ◽  
I Made Bakta ◽  
I Nyoman Mangku Karmaya ◽  
Bagus Komang Satriyasa ◽  
I Gusti Kamasan Nyoman Arijana
Keyword(s):  
Body Weight ◽  
Androgen Receptor ◽  
Treatment Group ◽  
Sertoli Cells ◽  
Carica Papaya ◽  
Ethanol Extract ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Control Group ◽  
Papaya Seeds

Background: Ethanol extract of young papaya seeds (Carica papaya L.) contains steroids that thought to has the antifertility property. This study aims to prove this hypothesis by measuring the low levels of testosterone hormone, spermatozoa count, and androgen receptor expression in sertoli cells. Material and Method:We use the randomized post-test only control group design on thirty-six male Balb/c strain adult male mice aged 12-14 weeks, weight 20-25 grams. They were randomly divided into a control and treatment group. The treatment group was given the same food as the control group plus ethanol extract of young papaya seeds 20 mg/20 gram body weight as much as 0.5 ml, orally every day for thirty-six days. Data were analyzed using the independent-sample T-test and Mann Whitney test. Result:The levels in the treatment group were significantly (p<0.05) lower than those in the control. Testosterone levels 31,64±1,91 vs 48,67±1,81 nmol/L, spermatozoa count 42,72±3,33 vs 75,89±4,71 cell/field of view, and androgen receptor expression 28,11±3.06% vs 55.07±2.49%. Conclusion:The ethanol extract of young papaya seeds 20 mg/20 g body weight has the antifertility property.

scholarly journals The effect of ant-plant (Hydnophytum formicarum) ethanol extract on collagen fibers for wound healing after tooth extraction in the guinea pig (Cavia cobaya)

2019 ◽  
Vol 31 (3) ◽  
pp. 189
Author(s):  
Siti Velanita ◽  
Efa Ismardianita ◽  
Andries Pascawinata
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Guinea Pig ◽  
Tooth Extraction ◽  
Healing Process ◽  
Ethanol Extract ◽  
Collagen Fibers ◽  
Control Group ◽  
Wound Healing Process ◽  
One Way Anova

Introduction: Tooth extraction causes tissue damage around the socket. Wound healing is essential for repairing damaged tissues. One of the medicinal plants often used is ant-plant (Hydnophytum formicarum), which contains flavonoids, triterpenoids, saponins and tannins. This research was aimed to determine the effect of ant-plant ethanol extract on the density of collagen fibers for wound healing after tooth extraction in the guinea pig (Cavia cobaya). Methods: The type of research was true experimental with a post-test only control group design. The study used 48 male guinea pig divided into 4 groups. Experimental groups received lower left incisor extraction, the control group was orally administered with 0.5% CMC, and the treatment group was administered with ant-plant extract dissolved with 0.5% CMC with dose of 4.65 mg, 6.2 mg, and 9.3 mg respectively; each guinea pig was given 3 cc of the extract 3 times a day until the termination day. On day 3, 7, 14, and 21 respectively, the guinea pigs were terminated and to make the histological preparations. The difference in collagen fibers density of the two observation groups was tested with one-way ANOVA, and the LSD test to analyse the significance between groups. Results: Application of ant-nest ethanol extract showed significant effects on the density of collagen fibers in the wound healing process after tooth extraction. Results of the one-way ANOVA test showed significant value on all treatment groups (p < 0.05). There were also found significant differences between the control group and the treatment group (p < 0.05). Conclusion: Ant-plant (Hydnophytum formicarum) ethanol extract increase the collagen fibers density on the wound healing after tooth extraction with the effective dose of 4.65 mg.Keywords: Ant-plant, Hydnophytum formicarum, wound healing, collagen, guinea pig, Cavia cobaya.

scholarly journals Histopathological Changes of Erythrocyte and Quantity of immature Erythrocytes in Tegal Duckling (Anas Javanica) Induced by Lead (Pb)

2010 ◽  
Vol 4 (1) ◽  
Author(s):  
Hamdani Budiman ◽  
Dwinna Aliza
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Clinical Symptoms ◽  
Young Female ◽  
Control Group ◽  
Histopathological Changes ◽  
Green Color ◽  
Treatment Groups ◽  
Erythrocyte Shape ◽  
Variant Analysis

The aim of this research is to find out the histopathological changes of erythrocyte and the quantity of immature erythrocyte of tegal duckling treated with lead (Pb). Twenty five young female ducks with the age of 7 days and 60.55.07 g body weight alloted into 4 treatment groups and 1 control group (Ko) with 5 replication each. The treatment group fed with K1 (5.7), K2 (11.4), K3 (17.1), and K4 (22.8) mg Pb/kg body weight for 10 days, respectively. Young ducks showed clinical symptoms such as the color of feces were brown to black, green color of diarrhea, anemic and decreasing body weight. Histopathologically, erythrocyte showed that immature and abnormal in shape (like teardrops, dumb bell, and bottles). The result of variant analysis followed by linier regression shows that the higher the dosage of Pb in diet the higher the quantity of immature erythrocyte andabnormal erythrocyte shape found (P0.01).

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