INFLUENCE OF INOCULUM SIZE ON PHYTASE PRODUCTION AND GROWTH IN SOLID–STATE FERMENTATION BY ASPERGILLUS NIGER

2001 ◽  
Vol 44 (4) ◽  
Author(s):  
C. Krishna ◽  
S. E. Nokes
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Inoculum Size ◽  
Phytase Production

scholarly journals Enhanced phytase production by Aspergillus niger mutants in solid state fermentation

2021 ◽  
Author(s):  
Shahzad Mahmood ◽  
◽  
Memuna G. Shahid ◽  
Muhammad Nadeem ◽  
Rubina Nelofer ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Research Work ◽  
Wild Strain ◽  
Chemical Mutagenesis ◽  
Poultry Feed ◽  
Control Group ◽  
Phytase Production

The present research work was conducted to improve the phytase production by genetic alteration of Aspergillus niger with induced mutagenesis using solid state fermentation. Strain improvement was carried out in the presence of ultra violet (UV) irradiation and ethylmethane sulphonate (EMS) [0.5% v/v] treatments for various time intervals. We reported an improved strain of Aspergillus niger designated as UV-3 mutant producing a zone of hydrolysis of about 40 mm, in comparison to wild strain (26 mm). The highest enzyme activity was found to be 547.64 IU/g for UV-3 mutant followed by EMS-4 mutant (492.23 IU/g)compared to wild strain which showed 406.45 IU/g of enzyme activity. There was 1.35 fold increase in phytase production after mutation studies of Aspergillus niger. Phytase was applied as poultry feed additive and given to broiler chickens for 5 weeks. The results exhibited that there was increase in body weight gain (BWG) of chicks for experimental group (2028 g) in comparison to control group (1903 g). Thus, physical and chemical mutagenesis was proved as an effective technique for the improvement of strain and ultimately for enhanced and economical phytase production for different industrial applications.

Research on Conditions of Solid-State Fermentation and Characters of Tannase by Aspergillus Niger B0201 with Gallnut

2011 ◽  
Vol 236-238 ◽  
pp. 1029-1038
Author(s):  
Xin Ying Wu ◽  
Shu Yi Qiu ◽  
Yang Zhen Li ◽  
Yu Xin Bao
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Culture Medium ◽  
Enzymic Activity ◽  
Inoculum Size ◽  
Optimum Temperature ◽  
Response Surface Design ◽  
Liquid Ratio ◽  
Surface Design

Study is on the solid-state fermentation of tannase with the Gallnut by Aspergillus niger B-0201.The experiments involved in the process and conditions of solid-state fermentation and factors influencing characters of tannase. The results indicated that the unpasteurized method before fermentation was in favor of the higher enzymic activity than the sterilized method. With the unpasteurized method, the tannase activity got the best level(51.2U/gds) ,when 5.0g of culture medium containing the 1g of Gallnut powder, 4g of the bran powder, 1% (w/w) of (NH4)2SO4, 0.1%(w/w) of NaCl, 0.1%(w/w) of MgSO4·7H2O, 8ml of water, 1.6:1 of the solid-to-liquid ratio and 1ml（1×108spores per milliliter）of inoculum size to a 250 ml conical flask was incubated at 30°C, initial pH6.0 for 96 hours. At the same time, the conditions of fermentation were further optimized using the Response Surface Design(RSM). The tannase activity got at 56.2U/gds with the .Gallnut(17%,w/w) and the solid-to-liquid ratio(1.5：1) in the medium under 32°C. The optimum temperature and pH of tannase was respectively 40°C and 5.0. Fe3+，Cu2+，Fe2+, Ba2+，Mn2+，Ca2+，Al3+were the inhibitor of tannase at 2.0mM concentration. The optimum concentration of propylgallate as the substrate catalyzed by Tannase was 1mM. The value of Kmwas 0.514mM and Vmaxwas 71.8μmol·(L·min)-1.

scholarly journals Phytase Production byAspergillus nigerCFR 335 andAspergillus ficuumSGA 01 through Submerged and Solid-State Fermentation

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Gunashree B. Shivanna ◽  
Govindarajulu Venkateswaran
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Enzyme Level ◽  
Solid Substrate ◽  
Aspergillus Ficuum ◽  
Phytase Production ◽  
Potato Dextrose Broth ◽  
Substrate Medium

Fermentation is one of the industrially important processes for the development of microbial metabolites that has immense applications in various fields. This has prompted to employ fermentation as a major technique in the production of phytase from microbial source. In this study, a comparison was made between submerged (SmF) and solid-state fermentations (SSF) for the production of phytase fromAspergillus nigerCFR 335 andAspergillus ficuumSGA 01. It was found that both the fungi were capable of producing maximum phytase on 5th day of incubation in both submerged and solid-state fermentation media.Aspergillus nigerCFR 335 andA. ficuumproduced a maximum of 60.6 U/gds and 38 U/gds of the enzyme, respectively, in wheat bran solid substrate medium. Enhancement in the enzyme level (76 and 50.7 U/gds) was found when grown in a combined solid substrate medium comprising wheat bran, rice bran, and groundnut cake in the ratio of 2 : 1 : 1. A maximum of 9.6 and 8.2 U/mL of enzyme activity was observed in SmF byA. nigerCFR 335 andA.ficuum, respectively, when grown in potato dextrose broth.

scholarly journals Optimization of Protease from Aspergillus Niger under Solid-State Fermentation Utilizing Shrimp Shell Substrate

2021 ◽  
Vol 11 (6) ◽  
pp. 14809-14824
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Proteolytic Enzymes ◽  
Initial Moisture Content ◽  
Inoculum Size ◽  
Protease Production ◽  
Shrimp Shell

Proteases, also known as proteinases or proteolytic enzymes, belong to a group of hydrolases. It can be applied in numerous fields and industries. Solid-state fermentation (SSF) is recognized as an effective method to produce protease. The ultimate aim of this study is to optimize the production of protease from Aspergillus niger under solid-state fermentation (SSF) by utilizing shrimp shell powder as a solid substrate. It was found that the produced protease from SSF was slightly alkaline. The correlation between factors operating parameters (incubation temperature, inoculum size, moisture content) for enzyme production is analyzed using statistical software, Minitab 16. A 23 full factorial experimental design was employed, and the enzyme produced was optimized by the method of desirability function. The optimal conditions for protease production of 3.7 U/mg were 35 °C of incubation temperature, 60% of initial moisture content, and 1.0 inoculum size. It is concluded that SSF protease was successfully produced from Aspergillus niger by utilizing shrimp waste as substrate. Through optimization study, moisture content, the interaction between incubation temperature and moisture content, interaction between moisture content and inoculum size significantly impact protease production.

scholarly journals Food Waste-Assisted Metal Extraction from Printed Circuit Boards: The Aspergillus niger Route

2021 ◽  
Vol 9 (5) ◽  
pp. 895
Author(s):  
Carlotta Alias ◽  
Daniela Bulgari ◽  
Fabjola Bilo ◽  
Laura Borgese ◽  
Alessandra Gianoncelli ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Food Waste ◽  
Solid State Fermentation ◽  
Printed Circuit Boards ◽  
Electronic Waste ◽  
Metal Extraction ◽  
Circuit Boards ◽  
Printed Circuit ◽  
Waste Printed Circuit Boards

A low-energy paradigm was adopted for sustainable, affordable, and effective urban waste valorization. Here a new, eco-designed, solid-state fermentation process is presented to obtain some useful bio-products by recycling of different wastes. Urban food waste and scraps from trimmings were used as a substrate for the production of citric acid (CA) by solid state fermentation of Aspergillus niger NRRL 334, with a yield of 20.50 mg of CA per gram of substrate. The acid solution was used to extract metals from waste printed circuit boards (WPCBs), one of the most common electronic waste. The leaching activity of the biological solution is comparable to a commercial CA one. Sn and Fe were the most leached metals (404.09 and 67.99 mg/L, respectively), followed by Ni and Zn (4.55 and 1.92 mg/L) without any pre-treatments as usually performed. Commercial CA extracted Fe more efficiently than the organic one (123.46 vs. 67.99 mg/L); vice versa, biological organic CA recovered Ni better than commercial CA (4.55 vs. 1.54 mg/L). This is the first approach that allows the extraction of metals from WPCBs through CA produced by A. niger directly grown on waste material without any sugar supplement. This “green” process could be an alternative for the recovery of valuable metals such as Fe, Pb, and Ni from electronic waste.

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