Short Communication: The genetic diversity and agronomical characters of local cultivars of tidal rice in East Kalimantan, Indonesia

2017 ◽  
Vol 18 (4) ◽  
Author(s):  
RUSDIANSYAH RUSDIANSYAH
Keyword(s):  
Genetic Diversity ◽  
Short Communication ◽  
Local Cultivars ◽  
East Kalimantan ◽  
Agronomical Characters

scholarly journals Short Communication: Genetic structure of Longtail Tuna Thunnus tonggol (Bleeker, 1851) in Java Sea, Indonesia

2020 ◽  
Vol 21 (8) ◽  
Author(s):  
M. DANIE AL MALIK ◽  
NI PUTU DIAN PERTIWI ◽  
ANDRIANUS SEMBIRING ◽  
NI LUH ASTRIA YUSMALINDA ◽  
ENEX YUNIARTI NINGSIH ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Short Communication ◽  
Management Plan ◽  
P Value ◽  
High Genetic Diversity ◽  
Population Structure Analysis ◽  
Fisheries Sustainability ◽  
Java Sea ◽  
Longtail Tuna

Abstract. Al Malik MD, Pertiwi NPD, Sembiring A, Yusmalinda NLA, Ningsing EY, Astarini IA. 2020. Short Communication: Genetic structure of Longtail Tuna Thunnus tonggol (Bleeker, 1851) in Java Sea, Indonesia. Biodiversitas 21: 3637-3643. Thunnus tonggol (Longtail Tuna) is an economically important fish found in Indonesia waters, however, the information regarding this fish is lacking. Known to be a neritic fish and found in shallow water, Java Sea is one of the ideal habitats for T. tonggol species. Due to high fishing rates activities in Java Sea, a better management plan to ensure the conservation and fisheries sustainability around this area is needed, especially to protect T. tonggol population. In order to complete the Indonesian tuna data, we aim to study the diversity and genetic structure of T. tonggol in Java Sea at three different locations; i.e. Semarang, Banjarmasin, and Jakarta. In this study, population genetic methods with the marker of mitochondrial DNA (mtDNA) control region were used in population structure analysis. A total of 115 specimens were collected from the fish market around the area of study locations and amplified using polymerase chain reaction (PCR) and sequenced using Sanger methods. The result showed genetic diversity (Hd) value of 0.99366, and nucleotide diversity (π) value of 0.01906. Both of these values indicated high genetic diversity. Population analyses using Analysis of Molecular Variance (AMOVA) showed nonsignificant differences between the three populations of study (mixing population), with the ΦST value of 0,00375 (p-value > 0.05). Based on this result, the fisheries management for T. tonggol in Java Sea needs to be managed as one single population management.

scholarly journals Short Communication: Identification and evaluation of bioactivity in forest plants used for medicinal purposes by the Kutai community of East Kalimantan, Indonesia

2018 ◽  
Vol 19 (1) ◽  
pp. 253-259 ◽  
Author(s):  
ABDUL RASYID ZARTA ◽  
WIWIN SUWINARTI ◽  
FARIDA ARIYANI ◽  
IRAWAN WIJAYA KUSUMA ◽  
ENOS TANGKE ARUNG
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Plant Species ◽  
Short Communication ◽  
Biological Effects ◽  
Bacterial Activity ◽  
Plant Materials ◽  
Vitro Assay ◽  
East Kalimantan ◽  
Forest Plants

Zarta AR, Ariyani F, Suwinarti W, Kusuma IW, Arung ET. 2018. Short Communication: Identification and evaluation of bioactivity in forest plants used for medicinal purposes by the Kutai community of East Kalimantan, Indonesia. Biodiversitas 19: 253- 259. The Indonesian forest is one of the most species-rich ecosystems in the world. Within such forests are plant species with secondary metabolites that have novel molecular structure and diverse biological activity with excellent potential to be used medicinally in prevention and cure of various diseases afflicting humans. Plant materials often contain various forms of antioxidants. Phenolic compounds found in plants have many biological effects. Flavonoids and other phenolics play a protective role against metabolic damage caused by disease and environmental stressors. The communities of Kutai Kartanegara in East Kalimantan Indonesia are representative of many traditional peoples who have evolved ways of treating human ailment and disease by use of specific plants sourced from their forests. The purpose of the research described in this paper was to identify significant medicinal plant species used by the Kutai ethnic community and to prepare extracts from these plants, mainly from the leaves, and to evaluate the extracts for bioactivity; namely by general identification of secondary metabolites, and by estimation of their antioxidant activity, toxicity, and antibacterial activity. Samples of ten plant species, used medicinally by the Kutai community, were extracted using ethanol solvent. Assay of antioxidant activity was carried out by the spectrophotometric method using DPPH (1,1-diphenyl-2-picrylhydrazyl radical) as the control. The degree of toxicity of the extracts was determined by the BSLT (Brine Shrimp Lethality Test) while anti-bacterial activity was evaluated using an in vitro assay of growth inhibition of cultures of the bacterium Escherichia coli. The result showed that nine of the plant species had strong antioxidant activity (IC50); extracts of two of the species were very toxic, while one other was toxic; and at least eight of the species had extracts that exhibited anti-bacterial activity. The phytochemical compounds identified in several of the ten species included flavonoids, tannins, saponins, steroids, triterpenoids, and alkaloids.

Short Communication: Genetic variation of Coelogyne pandurate, C. rumphii and their hybrids based on RAPD markers

2020 ◽  
Vol 21 (10) ◽  
Author(s):  
Sri Hartati ◽  
ENDANG S. MULIAWATI
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rapd Markers ◽  
Short Communication ◽  
F1 Hybrids ◽  
Polymorphic Bands ◽  
Large Clusters

Abstract. Hartati S, Muliawati ES. 2020. Short Communication: Genetic variation of Coelogyne pandurata, C. rumphii and their hybrids based on RAPD markers. Biodiversitas 21: 4709-4713. One effort to increase the genetic diversity of orchids is by crossing. This research aims to assess the genetic variation of a hybrid orchid obtained by crossing Coelogyne pandurata and C. rumphii and their hybrids based on RAPD markers. In this research, both parents were analyzed in three replications, while the hybrid was done in 10 replications. The study was conducted by analyzing DNA bands using RAPD markers with six primers, i.e. OPA 02, OPA 07, OPA 13, OPB 12, OPB 17, and OPD 08. Identification of the parents and their F1 hybrids showed 95.83% polymorphic bands with 43 bands measuring 200-2100 bp. The parents of C. rumphii, C. pandurata, and their hybrids showed similarity range of 0.16-1.00. The crossing of C. rumphii and C. pandurata resulted in a similarity of 0.5, shown in two large clusters. The first cluster consisted only of C. pandurata males and the second cluster consisted of C. rumphii females, together with all hybrid individuals. This study succeeded in creating new hybrids of orchids that have different characters from their parents, having a genetic variation of 23%.

scholarly journals Short Communication: Using RAPD technique to evaluate genetic diversity of longan (Dimocarpus longan) population in Vietnam

2017 ◽  
Vol 18 (4) ◽  
pp. 1632-1637
Author(s):  
VIET THE HO ◽  
QUOC NGUYEN NGO
Keyword(s):  
Genetic Diversity ◽  
Personal Experience ◽  
Genetic Background ◽  
Morphological Traits ◽  
Rapd Markers ◽  
Short Communication ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Conservation ◽  
Dimocarpus Longan

Ho VT, Ngo QNg. 2017. Short Communication: Using RAPD technique to evaluate genetic diversity of longan (Dimocarpus longan) population in Vietnam. Biodiversitas 18: xxxx. Longan (Dimocarpus longan L.) is an important fruit plant in Vietnam where several cultivars and landraces are being cultivated. Presently, the identification of longan cultivars and landraces is mainly based on personal experience relying on morphological traits leading the difficulty of genetic conservation and longan breeding. In this study, genetic richness and relativeness of 36 longan accessions collected from different provinces in Vietnam were evaluated by using 30 Random Amplified Polymorphic DNA (RAPD) primers. Our results reveal that there is the large variation of genetic background of studied longan accessions with separate groups in the obtained dendrogram. We also identified the combination of different RAPD markers could help to identify 15 longan genotypes. The results from this project could provide valuable information which is necessary for classifying, identifying plant origins, breeding and conserving programs of longan in Vietnam.

scholarly journals Short Communication: Genetic diversity of fumonisin producing Fusarium isolates from rice using PCR-RFLP of IGS-rDNA region

2018 ◽  
Vol 19 (2) ◽  
pp. 571-576
Author(s):  
SHUBHRANSU NAYAK ◽  
URMILA DHUA ◽  
APURBA CHHOTARAY ◽  
SOMA SAMANTA ◽  
CHANDAN SENGUPTA
Keyword(s):  
Genetic Diversity ◽  
Short Communication ◽  
Fusarium Species ◽  
Fusarium Verticillioides ◽  
Rice Varieties ◽  
Separate Branch ◽  
Pcr Rflp ◽  
Pcr Products ◽  
Silver Nitrate Staining ◽  
High Degree

Nayak S, Dhua U, Chhotaray A, Samanta S, Sengupta C. 2018. Short Communication: Genetic diversity of fumonisin producing Fusarium isolates from rice using PCR-RFLP of IGS-rDNA region. Biodiversitas 19: 571-576. Fusarium verticillioides (Sacc.) and related species produce carcinogenic mycotoxin known as Fumonisins in several agricultural crops including rice. However, this principal food crop has been infected by genetically diverse Fusarium species. Odisha belongs to the coastal part of India and many popular rice varieties are in the food chain in this region. Many Fusarium species producing fumonisins have been found to be associated with these rice varieties. Hence, the genetic diversity of twenty eight Fumonisin producers and non producers of Fusarium pathogens in this region was carried out in the current study. The IGS regions of 28 Fusarium isolates (both fumonisin producing and non producing) were amplified and the PCR products were restriction digested with ECoRI and HhaI. The digested products were separated on PAGE and bands were visualized by Silver Nitrate Staining. The 28 isolates could be separated into 14 IGS haplotypes. The lowest similarity was detected to be of 33% between F40 and F47. A group containing 14 isolates represented the biggest haplotypes. The isolates in which the FUM gene had not been detected (fumonisin non producer) were in a separate group having 90% similarity with each other and placed consistently in separate branch from others. Presence of unique band for this group was observed at 1650bp where as absence of specific bands was observed at 380bp and 300bp. The result of this study indicated a high degree of genetic variation among 28 Fusarium isolates. PCR RFLP of IGS region was also found to be useful for diversity study in Fusarium.

scholarly journals Short Communication: Genetic diversity of scalloped hammerhead sharks (Sphyrna lewini) landed in Muncar Fishing Port, Banyuwangi

2019 ◽  
Vol 20 (4) ◽  
pp. 1154-1159 ◽  
Author(s):  
FAQIH AKBAR ALGHOZALI ◽  
DIAH PERMATA WIJAYANTI ◽  
AGUS SABDONO
Keyword(s):  
Genetic Diversity ◽  
Short Communication ◽  
Polymerase Chain Reaction Amplification ◽  
Haplotype Diversity ◽  
Population Expansion ◽  
Fishing Ground ◽  
Sphyrna Lewini ◽  
Scalloped Hammerhead ◽  
Hammerhead Sharks ◽  
Longline Fisheries

Abstract. Alghozali FA, Wijayanti DP, Sabdono A. 2019. Short Communication: Genetic diversity of scalloped hammerhead sharks (Sphyrna lewini) landed in Muncar Fishing Port, Banyuwangi. Biodiversitas 20: 1154-1159. The majority of sharks caught in Indonesian fisheries were bycatch products from the tuna longline fisheries, but some regions in Indonesia fish the sharks as their main target. One of these regions is located in Muncar, Banyuwangi, which fishes the endangered Scalloped Hammerhead sharks (Sphyrna lewini) as target species. This research aimed to study the genetic diversity of the endangered Scalloped Hammerhead sharks landed in Muncar Fishing Port, Banyuwangi. Genetic analysis was done through PCR (Polymerase Chain Reaction) amplification and sequencing of the mitochondrial DNA COI (Cytochrome Oxidase subunit I) gene. Out of the 37 samples collected, 30 were successfully amplified and sequenced.The results showed moderate haplotype diversity (Hd: 0,582 ± 0,079) and low nucleotide diversity (π: 0,00392± 0,0024) with five haplotypes (h) and 26 polymorphic sites (S). Tajima’s D neutrality model values indicated a population expansion event. Two different clades were determined through phylogenetic analysis and by GenBank sequences comparison. These results provided basic information and present status of the Scalloped Hammerhead sharks population genetically within the fishing ground (Makassar Strait-Kangean Islands).

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