scholarly journals Short Communication: Identification of Leptin gene in crossbred beef cattle

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Laksa Ersa Anugratama ◽  
Tety Hartatik
Keyword(s):  
Beef Cattle ◽  
Phylogenetic Tree ◽  
Genetic Distance ◽  
Short Communication ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Bubalus Bubalis ◽  
Exon 2 ◽  
Central Java ◽  
Bali Cattle

Abstract. Anugratama LE, Hartatik T. 2020. Short Communication: Identification of Leptin gene in crossbred beef cattle. Biodiversitas 21: 226-230. Leptin is a gene that affects animal weight. Leptin gene is known to control body weight, feed intake, energy expenditure, immune function, and reproduction. This study aims to identify the diversity of the Leptin gene in crossbred beef cattle, Sumba Ongole cattle, Brahman cross cattle, Bali cattle, buffalo, sheep, and goat by comparing with four GenBank data of cattle. Crossbred beef cattle obtained from Klaten, Central Java, Indonesia. Leptin nucleotide sequences were analyzed using BioEdit to identify Single Nucleotide Polymorphism (SNP). To create amino acid change in Leptin gene, the coding sequence of exon 2 was established using BioEdit ver. 7.0.5. Phylogenetic tree and genetic distance have been analyzed based on the Leptin gene using MEGA 10.1.1 program. The result shows that eight variations of SNP were found in exon 2. The phylogenetic tree represents that crossbreed beef cattle, Sumba Ongole cattle, Brahman cross cattle, Bali cattle, Bos taurus, Bos indicus, Bos frontalis, Bos grunniens, Bubalus bubalis are in the same cluster with various genetic distance. The results of this study are expected to provide genetic information that will be used for further research on the relationship between Leptin gene polymorphisms to animal weight.

scholarly journals Phylogenetic tree analysis for Bali Cattle based on partial sequence 16S rRNA Mitochondrial DNA

2022 ◽  
Vol 335 ◽  
pp. 00014
Author(s):  
R. Misrianti ◽  
S.H. Wijaya ◽  
C. Sumantri ◽  
J. Jakaria
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
Phylogenetic Tree ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Partial Sequence ◽  
Bos Grunniens ◽  
Bos Javanicus ◽  
Mitochondria Dna ◽  
Bali Cattle

Mitochondria DNA (mtDNA) as a source of genetic information based on the maternal genome, can provide important information for phylogenetic analysis and evolutionary biology. The objective of this study was to analyze the phylogenetic tree of Bali cattle with seven gene bank references (Bos indicus, Bos taurus, Bos frontalis, and Bos grunniens) based on partial sequence 16S rRNA mitochondria DNA. The Bayesian phylogenetic tree was constructed using BEAST 2.4. and visualization in Figtree 1.4.4 (tree.bio.ed.ac.uk/software/figtree/). The best model of evolution was carried out using jModelTest 2.1.7. The most optimal was the evolutionary models GTR + I + G with p-inv (I) 0,1990 and gamma shape 0.1960. The main result indicated that the Bali cattle were grouped into Bos javanicus. Phylogenetic analysis also successfully classifying Bos javanicus, Bos indicus, Bos taurus, Bos frontalis and Bos grunniens. These results will complete information about Bali cattle and useful for the preservation and conservation strategies of Indonesian animal genetic resources.

scholarly journals Genetic diversity of Sragen Black Cattle based on D-Loop gene sequencing analysis

2020 ◽  
Vol 18 (2) ◽  
pp. 124
Author(s):  
Rahayu Kusumaningrum ◽  
Sutopo Sutopo ◽  
Edy Kurnianto
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Tree ◽  
Genetic Distance ◽  
Genetic Relationship ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Evolutionary Genetics ◽  
Sequencing Analysis ◽  
Dna Sequence Polymorphism ◽  
D Loop

<p class="MDPI17abstract"><strong>Objective: </strong>The objective of this study was to investigate the genetic diversity of Sragen Black Cattle based on D-loop sequences analysis.</p><p class="MDPI17abstract"><strong>Methods: </strong>A total of 25 blood samples belonged to Sragen Black Cattle that had no genetic relationship within sample. The DNA genome was extracted based on the manufacturer’s standard protocol using gSYNC DNA Mini Kit (Geneaid Biotech Ltd.). D-loop gene was amplified using specific primer forward: 5’- TAGTGCTAATACCAACGGCC-3’ and reverse: 5’- AGGCATTTTGAGTGCCTTGC-3’ and then was sequenced. The sequencing result was aligned and analyzed by Molecular Evolutionary Genetics Analysis (MEGA) version 6.0 to reveal genetic distance and phylogenetic tree. Genetic diversity and haplotype were analysed by DNA Sequence Polymorphism (DnaSp) v6.12.03.<strong></strong></p><p class="MDPI17abstract"><strong>Results: </strong>The results revealed that there were 11 haplotypes with Pi = 0.00675±0.00201 and Hd = 0.767±0.086. Sragen Black Cattle was divided by two cluster in phylogenetic tree with average of genetic distance was 0.0032.<strong></strong></p><p class="MDPI17abstract"><strong>Conclusions: </strong>In conclusion, all of Sragen Black Cattle are on the same cluster and have closer genetic relationship to Bos indicus rather than Bos taurus with similarity level 85.76 % based on BLAST program.</p>

scholarly journals Diversity of D-loop mitochondrial DNA (mtDNA) sequence in Bali and Sumba Ongole cattle breeds

2019 ◽  
Vol 44 (4) ◽  
pp. 335
Author(s):  
J. Jakaria ◽  
T. Musyaddad ◽  
S. Rahayu ◽  
M. Muladno ◽  
C. Sumantri
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Distance ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Haplotype Diversity ◽  
Complete Sequence ◽  
Distance Method ◽  
Mtdna Sequence ◽  
D Loop ◽  
Bali Cattle

This study aimed to investigate the diversity of the complete sequence of D-loop mitochondrial DNA (mtDNA) in Bali and Sumba Ongole (SO) cattlebreeds. A total of 24 blood samples were collected from Bali cattle (19 heads) and SO cattle (5 heads), and were extracted and then analyzed to obtain the sequence of D-loop mt DNA.Multiple alignments of the whole sequence of D-loop mtDNA were determined using clustal W. Genetic distance was calculated using a p-distance method, while the genetic tree was constructed using neighbor-joining (NJ) based on MEGA 6. Haplotype number, haplotype diversity (Hd) and nucleotide diversity (Pi) were analyzed using DnaSP version 6. As a result, the sequence of D-loop mtDNA in Bali cattle (921-1119 bp) and SO cattle (913 bp) was reported to have 8 and 4 haplotypes. Hd and Pi of Bali cattle reached 0.625±0.139 and 0.0266±0.0145, respectively, which wwere different from that of SO cattle, namely 0.900±0.1610 and 0.0064±0.0015, respectively. Specifically, we found 22 bp-repetitive nucleotide in Bali cattle, existing 3-9 times with a length of 66-198 bp present in D-loop mtDNA. This unique feature did not exist in SO cattle. Genetic distance and genetic tree determined according to sequence in hypervariability (HV-1) region of D-loop mtDNA (166 bp) resulted in satisfied separation, successfully classifying Bos javanicus, Bos indicus, and Bos taurus cluster.

scholarly journals Incidence of hereditary Citrullinemia and bovine Ieucocyte adhesion deficiency Syndrome in Indian dairy cattie (BOS TAURUS, BOS INDICUS) and buffalo (<i>BUBALUS BUBALIS</i>) Population (short communication)

1999 ◽  
Vol 42 (4) ◽  
pp. 347-352 ◽  
Author(s):  
M. Padeeri ◽  
K. Vijaykumar ◽  
S. Grupe ◽  
M. Pratp Narayan ◽  
M. Schwerin ◽  
...  
Keyword(s):  
Short Communication ◽  
Bos Taurus ◽  
Genetic Disorders ◽  
Bos Indicus ◽  
Bubalus Bubalis ◽  
Deficiency Syndrome ◽  
Argininosuccinate Synthetase ◽  
Indigenous Cattle ◽  
Holstein Bull ◽  
Holstein Bulls

Abstract. PCR based Screening of the cattle and buffaloe bulls for Bovine Lcucocyte Adhesion Deficiency Syndrome (BLAD) and Citrullinemia genetic disorders were carried out. A total of 330 cattle belonging to 4 breeds and 135 buffaloes of 3 breeds were genotyped. One young Holstein bull was detected as carrier (0.67%) for mutation at codon 86 in the argininosuccinate synthetase gene locus. Two young Holstein bulls were diagnosed as heterozygous (1.33%) for the BLAD locus. So far no carriers were detected in the indigenous cattle and buffalo breeds.

scholarly journals Phylogenetic tree of Kuantan cattle by DNA barcoding

2016 ◽  
Vol 21 (1) ◽  
pp. 41
Author(s):  
. Hidayati ◽  
R. Misrianti ◽  
A. Ali
Keyword(s):  
Beef Cattle ◽  
Phylogenetic Tree ◽  
Genetic Distance ◽  
Gene Segment ◽  
Bos Indicus ◽  
Dna Barcode ◽  
Cattle Breed ◽  
Coi Gene ◽  
Blood Samples ◽  
Primer Sequence

<p>Kuantan cattle is one of local beef cattle breed of Riau Province which its origin was unknown. Kuantan cattle are commonly found in Indragiri Hulu and Kuantan Singingi Regency. Based on phenotype characterizations, kuantan cattles are similar with pesisir cattle (West Sumatera beef cattle). Historically, kuantan cattle were pesisir cattle brought by “minang” immigrants (Immigrant from West Sumatera) to this region. The purpose of this study was to analyze the origin of the kuantan cattle through genetic diversity analysis using DNA barcode. DNA barcode used was Cytochrome oxidase subunit I gene which was found in the mtDNA. DNA isolation was done on 25 kuantan’s blood samples and 18 pesisir blood samples. Amplification of COI gene segment used Polymerase Chain Reaction technique. The forward primer sequence used in this study was F’5 TTCTCAACCAACCATAAAGATATTGG-3’ and the reverse primer sequence used was reverse 5’-TAGACTTCGGGGTGTCCAAAGAATCA-3. It squeezed kuantan and pesisir sequence 5711 - 6420 base (GeneBank accession number NC_005971) with length by 710 bp. Analysis result of sequence using MEGA 5.2 Program showed that there were 6 polymorphic sites establishing 7 haplotypes on kuantan cattle and 9 polymorphic sites establishing 12 haplotypes on pesisir cattle. Based on genetic distance and phylogeney tree, kuantan and pesisir cattle were in same group with <em>Bos indicus</em>. Mutation in the COI gene segment in this study was too small and was not able to distinguish the difference of those breeds. The result of neighbor joining analyze indicated that kuantan cattle origin was from <em>Bos indicus</em> just like pesisir cattle.</p><strong>Key Words: </strong>COI Gene, Polymorphic, Kuantan Cattle, Genetic Distance, Phylogenetic Tree

273 OVUM PICKUP AT DIFFERENT TIMES OF A SYNCHRONIZED FOLLICULAR WAVE DID NOT AFFECT IVP IN BOS INDICUS, BOS TAURUS, OR BUBALUS BUBALIS

2010 ◽  
Vol 22 (1) ◽  
pp. 293 ◽  
Author(s):  
L. U. Gimenes ◽  
M. L. Ferraz ◽  
A. Araujo ◽  
P. Fantinato Neto ◽  
M. R. Chiarati ◽  
...  
Keyword(s):  
Mixed Model ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Cumulus Cell ◽  
Bubalus Bubalis ◽  
Genetic Group ◽  
Cleavage Rate ◽  
Follicular Wave ◽  
Counting Data ◽  
Initial Hypothesis

One important factor in the success of ovum pickup (OPU)/IVP in Bos taurus is the follicular status at OPU concerning the dominance period (Hendriksen et al. 2000 Theriogenology 53, 11-20). The hypothesis of the present study is that OPU performed after follicle deviation, when follicles show a mild level of atresia, improves competence for IVP in Nelore (NE), Holstein (HO), and buffaloes (BU). Objectives were to determine effects of OPU done at different times of synchronized follicular wave (1, 3, or 5 d after expected emergence) and of genetic group (NE, HO, and BU) on IVP. A total of 27 heifers (9 of each genetic group) were maintained in contemporary nutritional and environmental conditions during experiment, in a cross-over design, performed in 6 replicates. Recovered oocytes with at least one cumulus cell layer were matured in TCM-199 supplemented with 10% of FCS plus 50 μM of cysteamin and 0.3 mM of cystine, at 38.5°C with 5% CO2 in air for 24 h. IVF was done with 2 × 106 spermatozoa per mL of NE (for bovine oocytes) or BU semen (for BU oocytes), for 20 h at the same incubator conditions of IVM. After IVF, presumptive zygotes were denuded and cultured in SOF under the same previous atmosphere conditions. Medium was changed 3 d after IVF when cleavage rate (CR) was assessed. Blastocyst (BR) and hatching rates (HR) were evaluated 7 and 9 days after IVF, respectively. About 50% of hatched blastocysts were fixed until nuclei counting. Data were analyzed by ANOVA using the Proc Mixed model. No effects of interaction or time of synchronization were observed in any of the variables. Concerning genetic group, NE had better results than HO and BU (mean ± SEM / heifer / replicate), respectively, for visualized follicles (41.0a ± 2.1, 22.1b ± 1.3, 18.8b ± 0.9), total oocytes (37.1a ± 2.5, 15.4b ± 1.2, 14.8b ± 1.0), oocytes at IVM (30.8a ± 2.4, 10.7b ± 1.0, 7.9b ± 0.7), oocytes at IVC (18.7a ± 0.8, 8.0b ± 0.5, 7.5b ± 0.4), cleaved embryos (15.4a ± 0.7, 4.6b ± 0.4, 4.4b ± 0.3),CR(81.8a, 59.1b, 62.3b), blastocysts on Day 7 (5.1a ± 0.6, 1.0b ± 0.2, 0.6b ± 0.1), BR (25.8a, 13.6b, 9.1b), and hatched blastocysts on Day 9 (2.6a ± 0.4, 0.3b ± 0.1, 0.3b ± 0.1). Recovery rate and HR were greater for NE (89.4 and 50.6%, respectively) than for HO (73.3 and 23.2%), but neither differed from BU (82.8 and 31.9%). Also, the percentage of viable was greater for NE (83.0) than for HO (66.9) and BU (53.1). No effects were observed for nuclei counting (NE = 176.6 ± 5.3, HO = 168.9 ± 13.7 and BU = 206.1 ± 23.1). Results demonstrate that Nelore had a better efficiency for IVP than Holstein and buffaloes. OPU performed at different times of synchronized follicular wave did not influence IVP, conversely to the initial hypothesis of this study. FAPESP (06/59550-6, 07/04782-2), Tortuga Cia Zootecnica®, Santa Adele and São Caetano Farms, LMMD, PCAPS, HOVET (Dr. Ubiraem Schalch), VRA, VNP (Prof. Dr. Francisco de Palma Rennó).

scholarly journals Clínica e hematologia de Bos indicus, Bos taurus e Bubalus bubalis inoculados com oocistos de Toxoplasma gondii (Apicomplexa: Toxoplasmatinae)

2001 ◽  
Vol 31 (4) ◽  
pp. 621-626 ◽  
Author(s):  
Francisco Carlos Rodrigues de Oliveira ◽  
Alvimar José da Costa ◽  
Gustavo Adolfo Sabatini
Keyword(s):  
Toxoplasma Gondii ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Bubalus Bubalis

Três animais de cada espécie (Bos indicus, Bos taurus e Bubalus bubalis) foram inoculados, via oral, com 2,0 x 10(5) oocistos de Toxoplasma gondii. Seis outros animais, dois de cada espécie, foram mantidos como testemunhas. As alterações clínicas surgidas a partir do 3º dia após inoculação (DAI) foram: hipertermia, taquicardia, taquipnéia, anorexia, prostração, corrimento nasal e lacrimejamento. Estes sinais foram mais evidentes nos taurinos, espécie que apresentou, ainda, diarréia, fotofobia e conjuntivite. Foi possível isolar T. gondii da corrente sangüínea em todas as espécies. Nos taurinos, a partir do 5º DAI até o final do experimento, o parasito foi isolado de todas as amostras de sangue colhidas semanalmente, com exceção do 14º, 35º e 63º DAI. Os bubalinos apresentaram parasitemia no 7º, 14º, 35º e 70º DAI e os zebuínos apenas no 7º e 28º DAI, correspondendo aos picos de temperatura, em todas as espécies, sendo mais evidente em taurinos. Os parâmetros clínico-laboratoriais demonstraram que os taurinos foram mais sensíveis ao T. gondii do que os zebuínos e estes não diferiram significativamente dos bubalinos, que tiveram aparente normalidade clínico-laboratorial.

scholarly journals Short Communication: A Polymorphism in ABCG2 in Bos indicus and Bos taurus Cattle Breeds

2006 ◽  
Vol 89 (12) ◽  
pp. 4921-4923 ◽  
Author(s):  
M. Ron ◽  
M. Cohen-Zinder ◽  
C. Peter ◽  
J.I. Weller ◽  
G. Erhardt
Keyword(s):  
Short Communication ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Cattle Breeds

scholarly journals Genetic Variation of Calpastation Gene of Indigenous Bali Cattle (Bos javanicus) in Indonesia

2019 ◽  
Vol 26 (1) ◽  
pp. 44
Author(s):  
Endang Tri Margawati ◽  
Slamet Diah Volkandari ◽  
Indriawati Indriawati ◽  
Emma M. Svensson
Keyword(s):  
Genetic Variation ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Meat Tenderness ◽  
Gene Markers ◽  
Bos Javanicus ◽  
Restriction Fragment Polymorphism ◽  
Pcr Rflp ◽  
Bali Cattle ◽  
Cast Gene

Calpastatin is one of gene markers affecting meat tenderness. The study aimed to evaluate genetic variation of calpastatin (CAST) gene of Bali cattle (Bos javanicus) in lndonesia. A total of 61 samples consisting of 21 Bali cattle, 22 Ongole cattle (Bos indicus), and 18 Friesian Holstein (FH) cattle (Bos taurus) were applied. The Ongole and FH cattle were involved for breed comparison. DNA was extracted from fresh blood using a High Salt method and measured their quality by a Spectrophotometer. A 523 bp of Calpastatin gene fragment was amplified by Polymerase Chain Reaction and Restriction Fragment Polymorphism (PCR-RFLP) technique with RsaI restriction enzyme for genotyping. Result showed that two variants alleles (C and G) and three genotypes (CC, GC, GG) were found in those Bali, Ongole and FH samples. Allele G was dominant allele with the highest G allele was in Bali cattle population (0.88). The higher percentage of allele C was found in Ongole and Friesian Holstein compared to that in Bali cattle. The Ongole breed tends to have a potential source of lean meat quality. This finding identified that genetic variation of CAST gene was exist in Bali cattle and adapted cattle of Ongole and FH in Indonesian.

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