scholarly journals Effects of supplementation with phosphorus, calcium and manganese during oil palm frond fermentation by Phanerochaete chrysosporium on ligninase enzyme activity

2020 ◽  
Vol 21 (5) ◽  
Author(s):  
Roni Pazla ◽  
Novirman Jamarun ◽  
Fauzia Agustin ◽  
Mardiati Zain ◽  
Arief Arief ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Phanerochaete Chrysosporium ◽  
Oil Palm ◽  
Manganese Peroxidase ◽  
Crude Protein ◽  
Lignin Degradation ◽  
Lignin Content ◽  
Oil Palm Frond ◽  
Range Test ◽  
Palm Frond

Abstract. Pazla R, Jamarun N, Agustin F, Zain M, Cahyani NO. 2020. Effects of supplementation with phosphorus, calcium and manganese during oil palm frond fermentation by Phanerochaete chrysosporium on ligninase enzyme activity. Biodiversitas 21: 1833-1838. The objective of this study was to evaluate the effects of supplementation with phosphorus (P) in combination with calcium (Ca) and manganese (Mn) during oil palm frond (OPF) fermentation by Phanerochaete chrysosporium on ligninase enzyme activity and lignin degradation. This study was carried out using a randomized complete design with 3 treatments (addition of P, Ca and Mn) and 5 replicates. The following treatments were performed: T1 (P 1000 + Ca 2000 + Mn 150 ppm), T2 (P 1500 + Ca 2000 + Mn 150 ppm), and T3  (P 2000 + Ca 2000 +Mn 150 ppm). The data were subjected to an analysis of variance (ANOVA), and differences between treatment means were tested using Duncan's multiple range test (DMRT). The parameters measured were as follows: lignin peroxidase (LiP) activity (U/mL), manganese peroxidase (MnP) activity (U/mL), crude protein (CP) content (%), crude fiber (CF) content (%) and the decrease in lignin (%). The results revealed a significant increase in LiP activity and CP content and a decrease in the lignin content (p<0.05) by the addition of P in the T3 treatment. However, the treatment nonsignificantly increased (p>0.05) MnP activity and significantly decreased (P<0.05) the CF content. In conclusion, supplementation of the OPF fermentation process with P 2000, Ca 2000, and Mn 150 ppm resulted in the highest ligninase enzyme activity and in decreased lignin content.

scholarly journals LIGNIN CONTENT IN FERMENTATION OF COCOA POD (THEOBROMA COCOA) USED PLEUROTUS OSTREATUS

2020 ◽  
Vol 2 (2) ◽  
pp. 1-7
Author(s):  
Engkus Ainul Yakin ◽  
Sri Sukaryani ◽  
Abbas Ummami
Keyword(s):  
Research Methods ◽  
Research Design ◽  
Pleurotus Ostreatus ◽  
Crude Protein ◽  
Dry Matter ◽  
Lignin Degradation ◽  
Pattern Analysis ◽  
Lignin Content ◽  
Range Test ◽  
Theobroma Cocoa

The study were aimed to determine the lignin degradation in fermentation of cocoa pod with Pleurotus ostreatus. The research methods was using three treatments and four replications. T0 = fermentation of cocoa pod in 10 days, T1 = fermentation of cocoa pod in 15 days, and T2 = fermentation of cocoa pod in 20 days. The mixture was put into a container aerobically. The variables observed were dry matter (DM), crude protein (CP), crude fiber (CF), crude fat (CFt), ash and lignin content. This study was designed using the completely randomized research design with a unidirectional pattern analysis of variance (oneway ANOVA). Significant variables went through Duncan's Multiple Range Test (DMRT). The result showed that the lower lignin content was T2 = fermentation of cocoa pod in 20 days = 6.40 ± 0.12%. It could be concluded that the addition of fungus Pleurotus ostreatus on cocoa pod fermented during 20 days was the lower lignin content in fermentation of cocoa pod.

Optimization of Oil Palm Fronds Pretreatment Using Ionic Liquid for Levulinic Acid Production

2014 ◽  
Vol 71 (1) ◽  
Author(s):  
Nur Aainaa Syahirah Ramli ◽  
Nor Aishah Saidina Amin ◽  
Ismail Ware
Keyword(s):  
Oil Palm ◽  
Levulinic Acid ◽  
Lignin Degradation ◽  
Process Condition ◽  
Temperature Reaction ◽  
Acid Yield ◽  
Box Behnken Design ◽  
Oil Palm Frond ◽  
Palm Fronds ◽  
Palm Frond

The pretreatment of oil palm frond (OPF) has been carried out using 1-butyl-3-methylimidazolium bromide ([BMIM]Br) in the presence of aqueous sulphuric acid (H2SO4). The effects of reaction temperature, reaction time and [BMIM]Br loading on lignin degradation were investigated by applying Box Behnken Design of Response Surface Methodology (RSM). The optimized process condition for OPF pretreatment were 123°C, 175 min and 9.9 g of [BMIM]Br loading with an optimum lignin degradation of 88.2%. The experimental results fitted-well with the predicted value with less than 5% error. It was also demonstrated that lignin degradation using recycled [BMIM]Br gave sufficient performance for five successive runs. It was revealed from SEM and XRD analyses, that the pretreated OPF was porous and less crystalline after pretreatment. Consequently, the pretreated OPF renders 25.3% levulinic acid yield in acid hydrolysis compared to 18.2% yield for untreated OPF.

scholarly journals Enzymatic Hydrolysis of Pretreated Fibre Pressed Oil Palm Frond by using Sacchariseb C6

2017 ◽  
Vol 206 ◽  
pp. 012008
Author(s):  
F S Hashim ◽  
H W Yussof ◽  
M A K M Zahari ◽  
R A Rahman ◽  
R M Illias
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Oil Palm Frond ◽  
Hydrolysis Of ◽  
Palm Frond

scholarly journals Investigation on the Dielectric Properties of Pulverized Oil Palm Frond and Pineapple Leaf Fiber for X-Band Microwave Absorber Application

2014 ◽  
Vol 893 ◽  
pp. 488-491 ◽  
Author(s):  
Elfarizanis Baharudin ◽  
Alyani Ismail ◽  
Adam Reda Hasan Alhawari ◽  
Edi Syams Zainudin ◽  
Dayang L.A. Majid ◽  
...  
Keyword(s):  
Dielectric Constant ◽  
Dielectric Properties ◽  
Oil Palm ◽  
Microwave Absorber ◽  
Band Frequency ◽  
Pineapple Leaf Fiber ◽  
X Band ◽  
Oil Palm Frond ◽  
Palm Frond ◽  
Leaf Fiber

This paper presents the results on dielectric properties of pulverized material based on agricultural waste namely oil palm frond and pineapple leaf fiber for microwave absorber application in the X-band frequency range. The investigation is started by identifying the pulverized materials permittivities and loss tangents using coaxial probe technique, followed by density measurement comprising the determination of bulk and solid densities. Then, by using dielectric mixture model, the solid particle dielectric properties were determined. It is observed that the air properties give quite an effect on the permittivity and loss tangent of the pulverized materials. It is also found that the lower the material density the higher material dielectric constant will be. Furthermore, the results show that, both oil palm frond and pineapple leaf fiber are potential to be X-band absorber with average dielectric constant of 4.40 and 3.38 respectively. The loss tangents for both materials were observed to be more than 0.1 which mark them as lossy materials.

Bioethanol Production from Oil Palm Frond Lignocellulose using Facultative Anaerobic Thermophilic Bacteria

2017 ◽  
pp. 171-183
Author(s):  
Siti Nur Ridhwah Muhamed Ramli ◽  
Tengku Elida Tengku Zainal Mulok ◽  
Sabiha Hanim Mohd Salleh ◽  
Khalilah Abdul Khalil ◽  
Othman Ahmad ◽  
...  
Keyword(s):  
Oil Palm ◽  
Thermophilic Bacteria ◽  
Bioethanol Production ◽  
Oil Palm Frond ◽  
Facultative Anaerobic ◽  
Palm Frond
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