scholarly journals Indigenous copper resistant bacteria isolated from activated sludge of water treatment plant in Surabaya, Indonesia

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Irawati Wahyu ◽  
REINHARD PINONTOAN ◽  
TRIWIBOWO YUWONO

Abstract. Irawati W, Pinontoan R, Yuwono T. 2020. Indigenous copper resistant bacteria isolated from activated sludge of water treatment plant in Surabaya, Indonesia. Biodiversitas 21: 5077-5084. Biological wastewater treatment using activated sludge is a promising wastewater treatment solution for removing heavy metals. To improve the effectiveness of biological wastewater treatment, activated sludge must consist of bacteria that can remove heavy metals through the process of bioaccumulation and biosorption. This study was aimed to isolate indigenous copper resistant bacteria and determining their resistance to copper, as well as analyzing their ability to accumulate and remove copper. Copper resistant bacteria were isolated from activated sludge of water treatment plant in industrial plant in Rungkut, Surabaya. Resistance to copper was analyzed by determining the value of minimum inhibitory concentration (MIC). The ability of bacterial isolates to remove copper was analyzed by atomic absorption spectrophotometer. A total of six highly copper resistant bacteria were isolated and designated as B6.1, C8.1, C9.3, C9.4, C9.5, C10.4 isolates. All isolates were categorized as high resistant bacteria with the MICs of 9-11 mM CuSO4. The two highest copper resistant bacteria were isolates C10.4 and C9.4. The ability of the two isolates to accumulate copper was 8.02 mg and 4.83 mg per gram dry weight of cells and to remove of copper up to 20.45% and 17.66%, respectively.

2015 ◽  
Vol 2 (1) ◽  
pp. 375
Author(s):  
Wahyu Irawati ◽  
Triwibowo Yuwono ◽  
Joedoro Soedarsono ◽  
Hari Hartiko

<p>Cupriavidus sp. IrC4 is a copper-resistant bacteria isolated from activated sludge in an Industrial Wastewater Treatment Plant in Rungkut-Surabaya, Indonesia. The purpose of this research was to study the potency of Cupriavidus sp. IrC4 as a bioremediation agent for copper, lead, mercury, and cadmium. Resistance of Cupriavidus sp. IrC4 to heavy metals were determined by measuring the minimum inhibitory concentration (MIC). Accumulation of copper, cadmium, and lead were determined by Atomic Absorption Spectrophotometer. Cupriavidus sp. IrC4 showed multiple resistance to heavy metals. The MICs of Cupriavidus sp. IrC4 to copper, lead, mercury, and cadmium were 16 mM, 15 mM, 6 mM, and 5 mM, respectively. The growth of Cupriavidus sp. IrC4 was inhibited by the addition of CuSO4 in the medium. The bacteria survived in the presence of high copper concentration as shown by the extension of the lag phase up to 36 hours. The analysis demonstrated that the copper resistance of the bacteria was facilitated through the accumulation of copper. Cupriavidus sp. IrC4 accumulated up to 367.78 and 260.01 mg/gram dry weight of cells of copper and lead, respectively. The bacteria demonstrated growth in the medium containing the mixture of 0.5 mM copper, lead, cadmium and accumulated those heavy metals up to 0.14, 24.74, and 12.49 mg/g dry weight of cells, respectively. The high resistance and capability of Cupriavidus sp. IrC4 to accumulate heavy metals can be exploited in bioremediation process for removing heavy metals from industrial sewage. </p><p><strong>Keywords</strong>: Accumulation, copper, Cupriavidus sp. IrC4,heavy metals, resistance.</p>


EKUILIBIUM ◽  
2011 ◽  
Vol 10 (1) ◽  
Author(s):  
Muljadi Muljadi

<p><strong><em>Abstract</em></strong><strong>: </strong><em>Batik industry in general is equipped with the Waste Water Treatment Plant (WWTP) used for wastewater treatment in order not to endanger the environment. Printed batik industry in Makamhaji, Sukoharjo equipped Wastewater Treatment Plant (WWTP) with the bar screen, sedimentation and coagulation-flocculation for wastewater treatment in the process is simple and economical to manufacture.The research objective was to determine the magnitude of performance and efficiency of industrial WWTP batik print with the bar screen, sedimentation, and the process of coagulation - flocculation of the reduction parameters COD, BOD and heavy metals Cr. The method used is an experimental method is to perform experiments on WWTP wastewater treatment industry in the village batik print Butulan Makamhaji Sukoharjo district.Of research that has been made </em><em></em><em>known that the greater efficiency resulting from the units of the performance of the unit means that the better. And obtain maximum efficiency of the reduction parameters of COD, BOD is the bar screen unit for 37.61% and 27.22%. As for the maximum efficiency of the reduction of Cr metal pollutant parameters are in units of coagulation-flocculation of 23.66%.</em></p><p><em> </em><strong><em>Keywords</em></strong><em>: COD, BOD, heavy metals chromium, Efficiency WWTP, WWTP Performance</em></p>


2016 ◽  
Vol 9 (2) ◽  
Author(s):  
Dinda Rita K. Hartaja ◽  
Imam Setiadi

Generally, wastewater of nata de coco industry contains suspended solids and COD were high, ranging from 90,000 mg / l. The high level of of the wastewater pollutants, resulting in nata de coco industry can not be directly disposed of its wastewater into the environment agency. Appropriate technology required in order to process the waste water so that the treated water can meet the environmental quality standards that are allowed. Designing the waste water treatment plant that is suitable and efficient for treating industrial wastewater nata de coco is the activated sludge process. Wastewater treatment using activated sludge process of conventional (standard) generally consists of initial sedimentation, aeration and final sedimentation.Keywords : Activated Sludge, Design, IPAL


2019 ◽  
Vol 24 (1) ◽  
pp. 135-163
Author(s):  
Jader Martínez Girón ◽  
Jenny Vanessa Marín-Rivera ◽  
Mauricio Quintero-Angel

Population growth and urbanization pose a greater pressure for the treatment of drinking water. Additionally, different treatment units, such as decanters and filters, accumulate high concentrations of iron (Fe) and manganese (Mn), which in many cases can be discharged into the environment without any treatment when maintenance is performed. Therefore, this paper evaluates the effectiveness of vertical subsurface wetlands for Fe and Mn removal from wastewater in drinking water treatment plants, taking a pilot scale wetland with an ascending gravel bed with two types of plants: C. esculenta and P. australis in El Hormiguero (Cali, Colombia), as an example. The pilot system had three upstream vertical wetlands, two of them planted and the third one without a plant used as a control. The wetlands were arranged in parallel and each formed by three gravel beds of different diameter. The results showed no significant difference for the percentage of removal in the three wetlands for turbidity (98 %), Fe (90 %), dissolved Fe (97 %) and Mn (98 %). The dissolved oxygen presented a significant difference between the planted wetlands and the control. C. esculenta had the highest concentration of Fe in the root with (103.5 ± 20.8) µg/g ; while P. australis had the highest average of Fe concentrations in leaves and stem with (45.7 ± 24) µg/g and (41.4 ± 9.1) µg/g, respectively. It is concluded that subsurface wetlands can be an interesting alternative for wastewater treatment in the maintenance of drinking water treatment plants. However, more research is needed for the use of vegetation or some technologies for the removal or reduction of the pollutant load in wetlands, since each drinking water treatment plant will require a treatment system for wastewater, which in turn requires a wastewater treatment system as well.


2010 ◽  
Vol 10 (5) ◽  
pp. 848-859 ◽  
Author(s):  
A. Uwimana ◽  
I. Nhapi ◽  
U. G. Wali ◽  
Z. Hoko ◽  
J. Kashaigili

A study was carried out to characterize the sludge produced at Kadahokwa Water Treatment Plant (KWTP) in Butare to assess the effectiveness of the sludge treatment and potential impacts of sludge disposal on the environment. Parameters analyzed were chromium, nickel, cadmium, lead, copper, zinc, iron, manganese, aluminium, total nitrogen, total phosphorus, potassium and cation exchange capacity (CEC). The results showed that 450±244.5 tons (dry weight) of sludge are produced annually. The concentrations of heavy metals in the sludge were below the standard limits for land application set by different countries. The high concentrations of nickel (42.3±2.5 ppm), chromium (29.9±6.2 ppm), cadmium (1.1±0.3 ppm) and lead (31.6±3.7 ppm) in the dried sludge posed a pollution risk for the wetland. The CEC was 28.4–33.3 cmol (+)/kg and pH was 6.50–7.45. It was concluded that the KWTP sludge is a poor source of total carbon, a moderate source of nutrients (NPK), and an important source of micronutrients, making it generally suitable for reuse for crop production. The CEC showed that the sludge could improve soil nutrient and water holding capacity. The higher concentration of aluminium (280 ppm) in the sludge creates an opportunity for recycling.


2006 ◽  
Vol 1 (4) ◽  
Author(s):  
N. Engelhardt ◽  
W. Lindner

With the commissioning of the waste water treatment plant Nordkanal, which has been dimensioned for a design capacity of 80,000 population equivalents, new worldwide standards for the implementation of large membrane-activated sludge plants have been created both from a technical and from an economic point of view. The hitherto successful operation of this plant has already now contributed towards this technology becoming suitable for use in large waste water treatment plants. The now two years the waste water treatment plant Nordkanal has been in operation have once again demonstrated that even on a large scale, membrane-activated sludge plants are able to reliably produce purified effluent of excellent quality, while simultaneously providing a small-sized design. They prove advantageous everywhere small-sized designs are sought after and the purified effluent has to meet high or special requirements. Wherever purification requirements are intensified in the foreseeable future, whether with regard to the hygienisation of effluent, or in the framework of re-using purified waste water as industrial water or potable water or in order to protect natural drinking water resources from critical anthropogenic impacts, the membrane bioreactor process or membrane filtration is trend setting and will increasingly gain in importance.


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