scholarly journals Evaluation of indigenous fungal entomopathogens and aqueous leaf extract of Annona muricata against Polyphagotarsonemus latus infesting Jatropha curcas in Indonesia

2021 ◽  
Vol 22 (7) ◽  
Author(s):  
Retno Dyah Puspitarini ◽  
AMINUDIN AFANDHI ◽  
ITO FERNANDO

Abstract. Puspitarini RD, Afandhi A, Fernando I. 2021. Evaluation of indigenous fungal entomopathogens and aqueous leaf extract of Annona muricata against Polyphagotarsonemus latus infesting Jatropha curcas in Indonesia. Biodiversitas 22: 2648-2655. The broad mite Polyphagotarsonemus latus (Banks) (Acari: Tarsonemidae) has been known to cause a great deal of injury on Jatropha curcas (L.) plantation in Indonesia. Isolation of indigenous fungal entomopathogens from rhizosphere soils, followed by pathogenicity assay was conducted to find an effective isolate for controlling P. latus. Additionally, the potential combination of the selected isolate and soursop (Annona muricata L.) aqueous leaf extract (SLE) was investigated. A total of 24 isolates were obtained by using the insect bait method. Four fungal isolates, namely Paecilomyces sp. 1., Lecanicillium sp., Beauveria sp., and Fusarium sp. 1, showed high conidial viability and were chosen as representatives to assess their pathogenicity against P. latus. Among the tested isolates, Beauveria sp. which had the highest conidial viability among the tested isolates, needed a shorter period to completely kill the tested mites. However, the compatibility test revealed the deleterious effect of SLE on Beauveria sp. SLE at all concentrations showed a very toxic effect on the fungus, therefore the two must be applied separately. Our results provide useful information on the effectiveness of indigenous entomopathogenic fungi Beauveria sp. and aqueous leaf extract of A. muricata as an alternative tool to control the broad mite P. latus on the J. curcas plantation.

Author(s):  
BEENA JOSE ◽  
FEMY THOMAS

Objective: The objective of the present study is the synthesis of iron oxide nanoparticles using Annona muricata aqueous leaf extract, characterization of the synthesized nanoparticles and evaluation of the antibacterial, photocatalytic activity and cytotoxicity. Methods: The iron oxide nanoparticle was synthesized using Annona muricata aqueous leaf extract and the crystal structure of the iron oxide nanoparticle was determined by UV-Visible spectroscopy, Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR). The in vitro cytotoxicity of iron oxide nanoparticles was evaluated using Dalton’s lymphoma ascites cells and the antibacterial assay was conducted using agar well diffusion method. Results: The UV-Visible spectrum of iron oxide nanoparticle showed a maximum absorption peak at 265 nm. This is the XRD pattern of iron oxide nanoparticles exhibited a distinct peak at 26.029 (2θ), accounting for crystal plane (211). SEM images revealed that the synthesized iron oxide nanoparticles were aggregated as irregular sphere shapes with rough surfaces. TEM image reveals the size of the synthesized iron oxide nanoparticles are spherical in shape with an average size of 20 nm. Green synthesized iron oxide nanoparticles using Annona muricata leaf extract effectively degraded methylene blue dye. Conclusion: This study showed that the synthesized iron oxide nanoparticles using Annona muricata aqueous leaf extract exhibited pronounced antibacterial, anticancer and photocatatytic activity and can be used in the textile industry for the purification of water contaminated with carcinogenic textile dyes. It can also be used as an external antiseptic in the prevention and treatment of bacterial infections.


2017 ◽  
Vol 12 (1) ◽  
pp. 1-6
Author(s):  
Enyi Obianuju ◽  
Okorie Pamela ◽  
Iyare Eghosa ◽  
Ugwu Princewill ◽  
Anyaehie Bond

2013 ◽  
Vol 134 (3) ◽  
pp. 327-332 ◽  
Author(s):  
L.E. Ferreira ◽  
P.M.N. Castro ◽  
A.C.S. Chagas ◽  
S.C. França ◽  
R.O. Beleboni

2015 ◽  
Vol 5 (1) ◽  
pp. 52-57
Author(s):  
M. Dangambo ◽  
H. Kabara ◽  
A. Alhassan ◽  
M. Sule ◽  
M. Atiku ◽  
...  

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Voravuth Somsak ◽  
Natsuda Polwiang ◽  
Sukanya Chachiyo

Malaria is one of the most important infectious diseases in the world. The choice for the treatment is highly limited due to drug resistance. Hence, finding the new compounds to treat malaria is urgently needed. The present study was attempted to evaluate the antimalarial activity of theAnnona muricataaqueous leaf extract inPlasmodium bergheiinfected mice. Aqueous leaf extract ofA. muricatawas prepared and tested for acute toxicity in mice. For efficacy testin vivo, standard 4-day suppressive test was carried out. ICR mice were inoculated with 107parasitized erythrocytes ofP. bergheiANKA by intraperitoneal injection. The extracts (100, 500, and 1000 mg/kg) were then given orally by gavage once a day for 4 consecutive days. Parasitemia, percentage of inhibition, and packed cell volume were subsequently calculated. Chloroquine (10 mg/kg) was given to infected mice as positive control while untreated control was given only distilled water. It was found thatA. muricataaqueous leaf extract at doses of 100, 500, and 1000 mg/kg resulted in dose dependent parasitemia inhibition of 38.03%, 75.25%, and 85.61%, respectively. Survival time was prolonged in infected mice treated with the extract. Moreover, no mortality to mice was observed with this extract up to a dose of 4000 mg/kg. In conclusion, theA. muricataaqueous leaf extract exerted significant antimalarial activity with no toxicity and prolonged survival time. Therefore, this extract might contain potential lead molecule for the development of a new drug for malaria treatment.


2021 ◽  
Vol 10 (3) ◽  
pp. 359-366
Author(s):  
Ana María Guevara-Vásquez ◽  
Julio Víctor Campos-Florián ◽  
Jesús Haydee Dávila-Castillo

Introduction: Poorly controlled hyperglycemia causes numerous health complications. Postprandial hyperglycemia is an important indicator of diabetic status. The aim of this research was to evaluate the effect of Annona muricata L. extract on the in vitro intestinal glucose absorption in diabetic rats and in vivo antihyperglycemic activity in both normal and diabetic rats. Methods: Phytochemical screening of the aqueous extract from the leaves of A. muricata was carried out. Albino rats were randomly assigned into normal and diabetic groups. Each group was divided into three subgroups: control (vehicle), experimental (A. muricata), and standard (Metformin) groups, to determine antihyperglycemic activity at different times after glucose overload. The everted intestinal sac technique was used to study intestinal glucose absorption in diabetic rats. Results: Aqueous leaf extract of Peruvian A. muricata exhibited statistically significant (P < 0.05) in vivo antihyperglycemic activity in both normal and diabetic rats when compared to the control group. The magnitude of the effect was similar to metformin treatment. Moreover, the aqueous leaf extract of A. muricata significantly diminished in vitro intestinal glucose absorption, with a magnitude similar to metformin treatment. Phytochemical analysis of the aqueous extract revealed the presence of tannins, flavonoids, alkaloids, and leucoanthocyanidins, among others. Conclusion: This study reveals that A. muricata aqueous extract is able to reduce in vitro intestinal glucose absorption and improve oral glucose tolerance in rats.


2014 ◽  
Vol 14 (1) ◽  
pp. 65-74 ◽  
Author(s):  
George Awuku Asare ◽  
Dan Afriyie ◽  
Robert A. Ngala ◽  
Harry Abutiate ◽  
Derek Doku ◽  
...  

Author(s):  
Maruvoor Arasi K ◽  
Krishnaveni C

 Objective: The objective of this study is to evaluate the potential of in vivo antipyretic activity of the aqueous leaf extracts of Annona muricata L. and Spermacoce articularis. L.f.Methods: The acute oral toxicity was determined by the Organization of Economic and Cooperation Development-423 class methods, and the in vivo antipyretic activity was determined by brewer’s yeast induced pyrexia method.Results: The results showed that the aqueous leaf extract of A. muricata. L and S. articularis L.f plants is non-toxic and possessed significant antipyretic effect.Conclusion: This study provides evidence for the antipyretic activity of A. muricata. L and S. articularis L.f. The aqueous leaf extract of S. articularis L.f at a dose of 400 mg/kg showed a more significant effect (p<0.01) in lowering the hypothermia than the extract of A. muricata L but found to have a similar effect as the standard drug aspirin (100 mg/kg).


2018 ◽  
Vol 2 (11) ◽  
pp. 489-493
Author(s):  
Olapeju Bolanle ◽  
◽  
Osakponmwen Oviasogie ◽  
Omonkhelin Owolabi ◽  
Abigail Akhigbemen ◽  
...  

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