scholarly journals Molecular identification and morphological description of Theloderma albopunctatum tadpoles from the Phu Khiao-Nam Nao Forest Complex, northeastern Thailand

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
Jidapa Chunskul ◽  
Prapaiporn Thongproh ◽  
Wanchai Simmasian ◽  
Jirachai Arkajag ◽  
Sataphon Thongpun ◽  
...  
Keyword(s):  
Bayesian Inference ◽  
Molecular Identification ◽  
National Park ◽  
Morphological Characters ◽  
Medium Size ◽  
Morphological Description ◽  
Wildlife Sanctuary ◽  
Molecular Analyses ◽  
Coi Sequences ◽  
Northeastern Thailand

Abstract. Chunskul J, Thongproh P, Simmasian W, Arkajag J, Tongpun S, Kanishthajata P, Prompalad S, Duangjai S, Duengkae P, Phochayavanich R, Chuaynkern C, Chuaynkern Y. 2021. Molecular identification and morphological description of Theloderma albopunctatum tadpoles from the Phu Khiao-Nam Nao Forest Complex, northeastern Thailand. Biodiversitas 22: 5145-5161. Presently, tadpole description of an external morphology and internal oral features of Theloderma albopunctatum (Anura, Rhacophoridae) is unknown. Therefore, this study aimed to describe tadpoles of T. albopunctatum from the Phu Khiao-Nam Nao Forest Complex in northeastern Thailand based molecular and morphological characters. Tadpole identification was based on molecular analyses. The 16S and COI sequences of the T. albopunctutatum tadpole had a genetic distance of 0% and 0.15% with the adults from Phu Luang Wildlife Sanctuary and Nam Nao National Park, respectively. Bayesian inference analyses of 16S, 16S+COI, and BDNF + SIA + RHO + TYR strongly supported the identity of tadpole and adults T. albopunctatum tadpoles are of medium size, with a black to ashy gray body that turns dark brown in preservative, they also exhibit elliptical body depressions and follow the keratodont row formula 1:2+2/1+1:2 or 1:1+1/1+1:1. T. albopunctatum tadpoles are generally similar to T. asperum.

scholarly journals Identification of the Pathogen Causing Stem Blight Disease on Chili in Sindangjaya Village, Cipanas, Cianjur, West Java Based on Morphological and Molecular Analyses

2021 ◽  
Vol 17 (1) ◽  
pp. 35
Author(s):  
Wartono Wartono
Keyword(s):  
Molecular Identification ◽  
Phytophthora Capsici ◽  
Economic Value ◽  
Morphological Characters ◽  
Stem Rot ◽  
West Java ◽  
Molecular Analyses ◽  
Molecular Approaches ◽  
Rot Disease ◽  
Blight Disease

<p>Chili (Capsicum annuum L.) is a vegetable commodity with high economic value which is widely cultivated by farmers in Indonesia. One of the obstacles faced in chili cultivation is stem rot disease. This study aimed to identify the pathogens that caused stem rot in chili plants obtained from one location in Sindangjaya Village, Cipanas District, Cianjur Regency, West Java Province based on morphological and molecular analyses. Pathogen identification was performed with morphological and molecular approaches. The morphological characters observed included colony shape, sporangium diameter, and mating type. The pathogenicity of the isolates was assayed by inoculating chili stems aged 40 days. Molecular identification was carried out using two pairs of primers for ITS regions and TEF-1 gene. Based on the results of morphological and molecular identification, as well as pathogenicity tests, it was confirmed that Phytophthora capsici pathogen was the causal agent of stem rot in chili plants collected from Sindangjaya Village. Further study is needed to determine the spread of the disease, damage, and yield loss caused by stem rot disease, as well as how to prevent and control the disease.</p>

A new skink of the genus Scincella Mittleman, 1950 (Squamata: Scincidae) from Ba Den Mountain, Tay Ninh Province, southern Vietnam

Zootaxa ◽  
2019 ◽  
Vol 4648 (2) ◽  
pp. 273-286
Author(s):  
SANG NGOC NGUYEN ◽  
VU DANG HOANG NGUYEN ◽  
LUAN THANH NGUYEN ◽  
ROBERT W. MURPHY
Keyword(s):  
New Species ◽  
Endemic Species ◽  
Morphological Characters ◽  
Nucleotide Sequences ◽  
Morphological Data ◽  
Medium Size ◽  
Southern Vietnam ◽  
Males And Females ◽  
Coi Sequences ◽  
Dorsal Pattern

Ba Den is an isolated mountain in southern Vietnam and home to two endemic species of lizards. Herein, we describe another endemic species, a new skink of the genus Scincella Mittleman, 1950, from the area based on morphological data, including hemipenial characters and nucleotide sequences of COI. The following morphological characters diagnose Scincella badenensis sp. nov.: medium size in adults (snout-vent length up to 64.4 mm); toes reach to fingers when limbs adpressed; midbody scale rows 32–36, smooth; paravertebral scales 67–71; dorsal scales not enlarged; ventral scale rows 68–74; supraoculars four; prefrontals in broad contact with one another; loreal scales two; tympanum deeply sunk, without auricular lobules; two enlarged anterior temporal scales; smooth lamellae beneath toe IV 18–20; pair of enlarged precloacal scales; hemipenes short, smooth and forked near the tip with two short lobes and two small terminal papillae; no dorsal pattern in males; and females with black interruptive vertebral line. The new species differs from its congeners by at least 10.4% uncorrected p-distance in COI sequences. 

A new karst-dwelling species of Kalophrynus (Anura: Microhylidae) from Gunung Mulu National Park, Borneo, Malaysia

Zootaxa ◽  
2011 ◽  
Vol 2737 (1) ◽  
pp. 49 ◽  
Author(s):  
J. MAXIMILIAN DEHLING
Keyword(s):  
National Park ◽  
Morphological Characters ◽  
Dominant Frequency ◽  
Medium Size ◽  
Advertisement Call ◽  
Adult Males ◽  
East Malaysia ◽  
Eye Diameter ◽  
A New Species ◽  
Reddish Orange

A new species of Kalophrynus is described from Gunung Mulu National Park in East Malaysia. The species is endemic to the karst hills and mountains of the Melinau Limestone Formation where it occurs at elevations between 70 and at least 1200 m. It differs from all other species of the genus by the combination of the following morphological characters: medium size (SVL of adult males 29.7–30.1 mm, adult females 35.5–38.8 mm), snout obtusely pointed, tympanum diameter slightly larger than half the eye diameter, upper jaw with weak dentition, fore limbs slender, Finger IV shorter than Fingers I and II, subarticular tubercles prominent, numbering one on Fingers I, II, and IV, and two on Finger III, a smaller, supernumerary metacarpal tubercle present between each finger and palmar tubercle, nuptial pads or asperities absent, heel reaching to posterior margin of eye when legs are adpressed to body, foot shorter than tibiofibula, Toe III longer than Toe V, toes webbed, inner and outer metatarsal tubercle prominent, dorsum and flanks black in life, separated from each other by white dorsolateral line running on both sides of body, chin and chest black, abdomen and proximal third of thigh grey in life, iris red to reddish-orange in life, advertisement call a single note consisting of 7 pulses and lasting 161 ms on average, with dominant frequency at 2250–2650 Hz.

scholarly journals Pollen morphology of Myrtaceae visited by social bees

2019 ◽  
Vol 6 (2) ◽  
pp. 98-105
Author(s):  
Andreia Santos do Nascimento ◽  
Carlos Alfredo Lopes de Carvalho
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Morphological Characters ◽  
Medium Size ◽  
Morphological Description ◽  
Pollen Spectrum ◽  
Social Bees ◽  
Equatorial Diameter ◽  
Polar Diameter

This study aimed to characterize species of Myrtaceae belonging to genera already identified in the pollen spectrum of beehive products of social bees, describing pollen grains morphology and correlating with taxonomic differences the species reported in the literature. For each plant species, measurements were made with 25 pollen grains in equatorial view and the polar diameter (PD) and equatorial diameter (ED) were measured. The exine thickness was obtained by measuring 10 pollen grains in polar view at the medium height of mesocolpium. The description of pollen morphology showed that pollen grains of the species are triangular amb, 3-colporates, psilate surface, small to medium size and oblate shape with the P/E ratio ranging from 0.52 to 0.72 µm. The morphological description of Myrtaceae species showed similarity between several characteristics common to pollen grains of this species. The description also allowed separation of nine species studied by size and specific morphological characters with the identification key, which can also be used to study the pollen spectrum of hive products.

Review of the genus Shilovia Makarchenko (Diptera: Chironomidae: Diamesinae: Boreoheptagyiini) from the mountains of Central Asia, with morphological description and DNA barcoding of known species

Zootaxa ◽  
2020 ◽  
Vol 4895 (2) ◽  
pp. 196-210
Author(s):  
EUGENYI A.  MAKARCHENKO ◽  
ALEXANDER A. SEMENCHENKO ◽  
DMITRY M. PALATOV
Keyword(s):  
Central Asia ◽  
Dna Barcoding ◽  
Morphological Study ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Description ◽  
Autonomous Region ◽  
Bayesian Inferences ◽  
East Kazakhstan ◽  
Coi Sequences

Chironomids of the genus Shilovia Makarchenko (Diamesinae, Boreoheptagyiini) from the mountains of Central Asia are revised using both morphological characters and molecular data. Illustrated descriptions of the adult male Shilovia xinhuawangi sp. nov. from Xinjiang Uygur Autonomous Region of China, S. yakovlevi sp. nov. from East Kazakhstan and redescription of S. rara Makarchenko from Tajikistan and Kyrgyzstan are provided. The result of morphological study is congruent with DNA barcoding analyses using COI sequences. The average K2P interspecific nucleotide distances within S. xinhuawangi sp. nov. and S. yakovlevi sp. nov. are 0.03% and 0.3% respectively. The nucleotide distances between the two new species and S. rara can be considered interspecific. Phylogenetic analysis using Maximum likelihood (ML) and Bayesian inferences (BI) support the placement of S. xinhuawangi sp. nov. and S. yakovlevi sp. nov. within the monophyletic genus Shilovia. 

scholarly journals Newly Emerging Pest in China, Rhynchaenus maculosus (Coleoptera: Curculionidae): Morphology and Molecular Identification with DNA Barcoding

Insects ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 568
Author(s):  
Rui-Sheng Yang ◽  
Ming-Yang Ni ◽  
Yu-Jian Gu ◽  
Jia-Sheng Xu ◽  
Ying Jin ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Species Identification ◽  
Molecular Identification ◽  
Morphological Characters ◽  
Information Storage ◽  
Morphological Description ◽  
Quick Response ◽  
Dna Barcodes ◽  
Pairwise Divergence ◽  
Coding Capacity

The oak flea weevil, Rhynchaenus maculosus Yang et Zhang 1991, is a newly emerging pest that severely damages oak (genus Quercus) in China. The first R. maculosus outbreak occurred in 2020 and caused spectacular damage to all oak forests in Jilin province, northeast China. The lack of key morphological characters complicates the identification of this native pest, especially in larva and pupa stages. This is problematic because quick and accurate species identification is crucial for early monitoring and intervention during outbreaks. Here, we provided the first detailed morphological description of R. maculosus at four life stages. Additionally, we used DNA barcodes from larva and pupa specimens collected from three remote locations for molecular identification. The average pairwise divergence of all sequences in this study was 0.51%, well below the 2% to 3% (K-2-parameter) threshold set for one species. All sample sequences matched the R. maculosus morphospecies (KX657706.1 and KX657707.1), with 99.23% to 100% (sequence identity, E value: 0.00) matching success. The tree based on barcodes placed the specimens into the Rhynchaenus group, and the phylogenetic relationship between 62 sequences (30 samples and 32 from GeneBank) had high congruence with the morphospecies taxa. The traditional DNA barcodes were successfully transformed into quick response codes with larger coding capacity for information storage. The results showed that DNA barcoding is reliable for R. maculosus identification. The integration of molecular and morphology-based methods contributes to accurate species identification of this newly emerging oak pest.

An updated generic circumscription for Cryptangieae (Cyperaceae, Poales) based on a molecular phylogeny and a morphological character reconstruction

Phytotaxa ◽  
2021 ◽  
Vol 483 (3) ◽  
pp. 211-228
Author(s):  
SUZANA M. COSTA ◽  
FABIO A. VITTA ◽  
WILLIAM W. THOMAS ◽  
A. MUTHAMA MUASYA ◽  
ROSEMERI MOROKAWA ◽  
...  
Keyword(s):  
Bayesian Inference ◽  
Molecular Phylogeny ◽  
Morphological Character ◽  
Morphological Characters ◽  
New Combinations ◽  
Phylogenetic Significance ◽  
Generic Level ◽  
Molecular Analyses ◽  
Character Reconstruction ◽  
The Relationship

Cryptangieae is a monophyletic tribe, as asserted in a recent molecular hypothesis, but there are questions about the circumscription and relationships of its genera. We enlarged the sampling of the tribe diversity, including about 80% of known species, and provide new analyses using single and combined matrices from two chloroplast (rbcL and trnL-F) and three nuclear ribosomal (ITS, ETS and 5S-NTS) regions with Bayesian Inference to clarify these questions. We also performed character reconstruction analyses with diagnostic morphological characters, including some traditionally applied in Cryptangieae taxonomy, using parsimony methods. The molecular analyses show Lagenocarpus in the current circumscription as polyphyletic, and the need to merge Cephalocarpus and Everardia to avoid paraphyletic genera. We propose an updated circumscription following the phylogeny, including a new name to a group at generic level (Krenakia); and discuss the phylogenetic significance of the morphological characters in Cryptangieae taxonomy. Though the relationship among some genera still needs more research, the genera we propose are strongly supported clades with clear morphological synapomorphies. Additionally, we provide an identification key, a brief description of genera and a list of accepted species including the required new combinations.

An African glass frog: A new Hyperolius species (Anura: Hyperoliidae) from Nyungwe National Park, southern Rwanda

Zootaxa ◽  
2012 ◽  
Vol 3391 (1) ◽  
pp. 52 ◽  
Author(s):  
J. MAXIMILIAN DEHLING
Keyword(s):  
New Species ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
National Park ◽  
Morphological Characters ◽  
Medium Size ◽  
Advertisement Call ◽  
Rrna Gene ◽  
Adult Males ◽  
Mitochondrial 16S Rrna Gene

A new species of Hyperolius is described from Nyungwe National Park in southern Rwanda. The new species differs fromits congeners by a unique combination of morphological characters, including a light green dorsum and a transparentventral skin resembling glass frogs of the Neotropical family Centrolenidae, blue-coloured bones, reduced toe webbing,relatively wide head, acuminate snout, small to medium size (SUL of adult males 18.8–23.2 mm), and the presence ofnuptial pads. The advertisement call differs from all calls of other species of the genus that have been analyzed.Comparison of the mitochondrial 16S rRNA gene showed a relatively close relationship to H. castaneus, H.cystocandicans, H. discodactylus, H. frontalis, and H. lateralis. The 16S sequence of the new species differs by at least 4.5% in the uncorrected p-distance from all available sequences of other species of the genus.Ubwoko bushya bwa Hyperolius buboneka muri parike nasiynali ya Nyungwe mu majyepfo yu Rwanda. Ubu bwokobushya butandukaniye na bugenzi bwabwo gusa kurusobe rw’miterere yabwo, harimo dorsum ifite ibara ry’cyatsi cyeru-rutse n’ruhu rubonerana rwo kunda, amagufwa afite ibara ry’bururu, n’tunodufatanijwe n’gahu, umutwe wenda kubamunini, umunwa uurungushuye, umubyimba uri hagati ya 18,8 mm kugera kuri 23,3 mm ku ngabo nkuru, ikagira n’mag-aragamba ku ruhu. Kuzitangaza kwazo byagiye bitandukana n’matangazo yabaye kubundi bwoko bwazo. Ugereanijen’miterere yazo idahinduka (mitochondrial 16S rRNA gene) niyizindi usanga bifitanye isano ya bugufi na H. castaneus,H.cystocandicans, H.discodactylus, H.frontalis na H. lateralis. Urukurikirane rwa 16S y’bu bwoko bushya rutandukanye kuri 4,5% ugereranije n’nkurikirana zabaye z’bundi bwoko busa n’bu.

A new skink of the genus Sphenomorphus Fitzinger, 1843 (Squamata: Scincidae) from Hon Ba Nature Reserve, southern Vietnam

Zootaxa ◽  
2018 ◽  
Vol 4438 (2) ◽  
pp. 313
Author(s):  
SANG NGOC NGUYEN ◽  
LUAN THANH NGUYEN ◽  
VU DANG HOANG NGUYEN ◽  
NIKOLAI L. ORLOV ◽  
ROBERT W. MURPHY
Keyword(s):  
New Species ◽  
Nature Reserve ◽  
Tail Length ◽  
Morphological Characters ◽  
Length Ratio ◽  
Medium Size ◽  
Southern Vietnam ◽  
Gene Coi ◽  
New Forest ◽  
Coi Sequences

A new forest skink of the genus Sphenomorphus Fitzinger, 1843 is described from Khanh Hoa Province, southern Vietnam based on morphological characters of four specimens and a fragment of 653 nucleotides of the gene COI. Sphenomorphus yersini sp. nov. is characterized by the following morphological characters: medium size in adults (snout-vent length up to 55 mm); tail length/snout-vent length ratio 1.81; toes reach to fingers when limbs adpressed; midbody scale rows 32–34, smooth; paravertebral scales 61–69; ventral scale rows 58–67; subcaudal scales 112; supraoculars four, rarely five; prefrontals in broad contact with one another; loreal scales two; tympanum deeply sunk; smooth lamellae beneath finger and toe IV 10–12 and 18–20 respectively; a pair of enlarged precloacal scales; hemipenis deeply forked and asymmetrical with two differently sized smooth lobes. The new species differs from its most similar congener, Sphenomorphus buenloicus Darevsky & Nguyen, 1983, by 16.4–16.7% uncorrected p-distance in COI sequences. 

scholarly journals Prey items of some amphibians and reptiles in Phu Khieo–Nam Nao Forest Complex, northeastern Thailand

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
Prapaiporn Thongproh ◽  
JIDAPA CHUNSKUL ◽  
PEERASIT RONGCHAPHO ◽  
CHANTIP CHUAYNKERN ◽  
YODCHAIY CHUAYNKERN ◽  
...  
Keyword(s):  
Direct Observation ◽  
Prey Item ◽  
National Park ◽  
Wildlife Sanctuary ◽  
First Report ◽  
Observation Method ◽  
Direct Observation Method ◽  
Prey Items ◽  
Fejervarya Limnocharis ◽  
Northeastern Thailand

Abstract. Thongproh P, Chunskul J, Rongchapho P, Chuaynkern C, Chuaynkern Y, Srisonchai R, Saengsri C, Aonpime P, Phochayavanich R, Kanishthajata P, Phusaensri S, Prompalad S, Tongpun S, Arkajag J, Duengkae P. 2020. Prey items of some amphibians and reptiles in Phu Khieo–Nam Nao Forest Complex, Northeastern Thailand. Biodiversitas 21: 4124-4130. We reported prey items for two amphibians (Fejervarya limnocharis and Sylvirana nigrovittata) and three reptiles (Hebius sp., Bungarus candidus and Xenopeltis unicolor) from several areas of Phu Khieo–Nam Nao Forest Complex (northeastern Thailand) based on direct observation method. Fejervarya limnocharis (Anura: Dicroglossidae) consumed Amynthas sp. (Clitellata: Megascolecidae) in Nam Nao National Park (Phetchabun Province), while Sylvirana nigrovittata (Anura: Ranidae) consumed Megaustenia sp. (Gastropoda: Ariophantidae) in Phu Long Forest (Chaiyaphum Province). Consumption of snails in Sylvirana nigrovittata was documented as the first report on this prey item for the species. In Phu Luang Wildlife Sanctuary (Loei Province), Hebius sp. (Serpentes: Colubridae) consumed Fejervarya limnocharis while Bungarus candidus (Serpentes: Elapidae) consumed Argyrophis muelleri (Serpentes: Typhlopidae). Xenopeltis unicolor (Serpentes: Xenopeltidae) consumed Glyphoglossus molossus (Anura: Microhylidae) in Phu Wiang National Park (Khon Kaen Province).

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