scholarly journals Adenoviral Gizzard Erosion in Commercial Broiler Chickens

2003 ◽  
Vol 40 (3) ◽  
pp. 294-303 ◽  
Author(s):  
M. Ono ◽  
Y. Okuda ◽  
S. Yazawa ◽  
Y. Imai ◽  
I. Shibata ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Inclusion Bodies ◽  
Broiler Chickens ◽  
Plasma Cells ◽  
Muscle Layer ◽  
Intranuclear Inclusion ◽  
Group I ◽  
Serotype 1 ◽  
Commercial Broiler ◽  
Antibody Levels

Pathologic and immunohistochemical changes caused by group I of the fowl adenovirus (FAV) serotype-1 99ZH strain, isolated from broiler chickens exhibiting gizzard erosion, were investigated in commercial broiler chickens. One hundred twenty-two chickens were inoculated with the strain by both oral and ocular routes at 1, 3, or 5 weeks of age and euthanatized for necropsy within 4–18 days of inoculation. Focal gizzard erosions were observed in the inoculated chickens of each age group. A histologically degenerative koilin layer, necrotic mucosa, intranuclear inclusion bodies in the glandular epithelial cells, inflammatory cell infiltrations in the lamina propria, submucosa, and a muscle layer were seen in the gizzards. Immunohistochemical staining showed evidence of FAV antigens in the intranuclear inclusion bodies. These findings were recognized regardless of their maternal antibody levels for FAV serotype-1. Gizzard lesions appeared later in the lower-dose-inoculated chickens than in the higher-dose–inoculated chickens. Numerous CD3-positive cells and IgY-positive plasma cells were seen in the gizzard lesions. In 5-week-old chickens the heterophil infiltrations in the lesions were milder than in younger chickens. Intranuclear inclusion bodies also were observed in the epithelial cells of the ileum or cecal tonsils of some chickens. Thus, this study shows that FAV-99ZH causes adenoviral gizzard erosion in broiler chickens without hepatic or pancreatic lesions and that cell infiltration is more severe than in dietary gizzard erosions.

scholarly journals Quantitative profiling of the shedding rate of the three Marek's disease virus (MDV) serotypes reveals that challenge with virulent MDV markedly increases shedding of vaccinal viruses

2007 ◽  
Vol 88 (8) ◽  
pp. 2121-2128 ◽  
Author(s):  
Aminul Islam ◽  
Stephen W. Walkden-Brown
Keyword(s):  
Disease Virus ◽  
Broiler Chickens ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Commercial Broiler ◽  
Virulent Virus

The shedding profile of Marek's disease virus serotype 1 (MDV1, virulent), serotype 2 (MDV2, vaccinal) and herpesvirus of turkeys (HVT, vaccinal) in commercial broiler chickens was determined by measuring the daily rate of production of feather dander from chickens housed in isolators and by quantifying the viral load of each of these serotypes in the dander using quantitative real-time PCR (qPCR). MDV1 and HVT viruses were detectable in dander filtered from isolator exhaust air from day 7 and MDV2 from day 12 after infection and thereafter until the end of the experiment at 61 days of age of the chickens. There was no difference in shedding rate among the three MDV1 isolates. Daily shedding of MDV1 increased sharply between days 7 and 28 and stabilized thereafter at about 109 virus copies per chicken per day, irrespective of vaccination status. Challenge with the three different MDV1 isolates markedly increased shedding of the vaccinal viruses HVT and MDV2 in dander by 38- and 75-fold, respectively. These results demonstrate the utility of qPCR for the differentiation and quantification of different MDV serotypes in feather dander and have significant implications for the routine monitoring of Marek's disease using qPCR assays of dust, for epidemiological modelling of the behaviour and spread of MDVs in chicken populations and for studies into the evolution of virulence in MDV1 in the face of blanket vaccination with imperfect vaccines that ameliorate disease but do not prevent infection and replication of virulent virus.

Observations on the Ultrastracture of Baculovirus in Australian Penaeus monodon and Penaeus merguiensis

1988 ◽  
Vol 39 (6) ◽  
pp. 743 ◽  
Author(s):  
A Doubrovsky ◽  
JL Paynter ◽  
SK Sambhi ◽  
JG Atherton ◽  
RJG Lester
Keyword(s):  
Epithelial Cells ◽  
Digestive Gland ◽  
Inclusion Bodies ◽  
New South ◽  
New South Wales ◽  
Penaeus Monodon ◽  
Northern Territory ◽  
Intranuclear Inclusion ◽  
South Wales ◽  
Penaeus Merguiensis

Baculovirus particles were found in the digestive gland of Penaeus monodon from hatcheries and grow-out ponds in northern New South Wales, northern Queensland and the Northern Territory. Similar particles were seen in a Penaeus merguiensis specimen caught off Townsville, Queensland. The particles were found in the hypertrophied nuclei of hepatopancreatic epithelial cells, both free in the nucleoplasm and occluded in large eosinophilic intranuclear inclusion bodies. The nucleocapsids of the particles measured 45-52 nm × 260-300 nm and resembled the baculovirus reported from Penaeus plebejus.

scholarly journals Gastrointestinal Pathogenicity of Adenoviruses and Reoviruses Isolated from Broiler Chickens in Alabama

1998 ◽  
Vol 10 (2) ◽  
pp. 145-151 ◽  
Author(s):  
Stephen D. Lenz ◽  
Frederic J. Hoerr ◽  
Alfred C. Ellis ◽  
Maria A. Toivio-Kinnucan ◽  
Maria Yu
Keyword(s):  
Inclusion Bodies ◽  
Broiler Chickens ◽  
Digestive System ◽  
Necrotizing Pancreatitis ◽  
Bursa Of Fabricius ◽  
Pancreatic Acinar Cells ◽  
Control Group ◽  
Specific Pathogen ◽  
Commercial Broiler ◽  
Viral Inclusion

Adenoviruses and reoviruses isolated from commercial broiler chickens were evaluated for gastrointestinal pathogenicity in specific-pathogen-free Leghorn chickens. The viruses were originally isolated from either the proventriculus or a gastrointestinal pool of tissues of broiler chickens with proventriculitis or enteritis. Isolates were cloned by terminal dilution. Day-old chickens were inoculated by oral and ocular routes with undiluted tissue culture fluids (titers of 102-104 TCID50/ml) and then examined at necropsy on days 5, 10, and 15 postinoculation. Chickens in all virus groups (but not the control group) developed wet, unformed fecal droppings that persisted for the duration of the study. Mild lesions occurred in reovirus-inoculated chickens and included hyperplasia of lymphocyte aggregates in various organs and mild gizzard erosions. Chickens inoculated with adenovirus isolates developed marked gizzard erosions and necrotizing pancreatitis as well as mild proventriculitis. Intranuclear viral inclusion bodies occurred in gizzard epithelium and pancreatic acinar cells at the sites of lesions. Lymphocytic atrophy occurred in the bursa of Fabricius. Respective viruses were reisolated from proventriculus and duodenum collected from chickens of each group; no viruses were isolated from controls. Under the conditions of this study, adenovirus isolates were more pathogenic than the reovirus isolates in the digestive system.

Diseases of anserif ormes associated with intranuclear inclusion bodies in epithelial cells

1987 ◽  
Vol 64 (9) ◽  
pp. 290-291 ◽  
Author(s):  
R L REECE ◽  
D A BARR ◽  
R T BADMAN ◽  
J HILI ◽  
S McORIST
Keyword(s):  
Epithelial Cells ◽  
Inclusion Bodies ◽  
Intranuclear Inclusion

scholarly journals Molecular characterization of fowl adenovirus group I in commercial broiler chickens in Brazil

VirusDisease ◽  
2018 ◽  
Vol 29 (1) ◽  
pp. 83-88 ◽  
Author(s):  
David De la Torre ◽  
Luis F. N. Nuñez ◽  
Silvana H. Santander Parra ◽  
Claudete S. Astolfi-Ferreira ◽  
Antonio J. Piantino Ferreira
Keyword(s):  
Molecular Characterization ◽  
Broiler Chickens ◽  
Fowl Adenovirus ◽  
Group I ◽  
Commercial Broiler

Intranuclear Inclusion Bodies in Epithelial Cells of Human Vas Deferens

1979 ◽  
Vol 2 (1) ◽  
pp. 1-12 ◽  
Author(s):  
J. Chakraborty ◽  
L. Nelson ◽  
J. Jhunjhunwala ◽  
M. Young ◽  
K. Kropp
Keyword(s):  
Epithelial Cells ◽  
Inclusion Bodies ◽  
Vas Deferens ◽  
Intranuclear Inclusion

scholarly journals ​Effect of Feeding Corn Gluten Meal in Feed Ration on Growth Performance of Commercial Broiler Chicken

2021 ◽  
Author(s):  
K. Abhijeet ◽  
S.B. Prasanna ◽  
P.S. Mahesh ◽  
R.Y. Ranjith ◽  
Prabha Karan ◽  
...  
Keyword(s):  
Weight Gain ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Feed Consumption ◽  
Corn Gluten Meal ◽  
Group I ◽  
Corn Gluten ◽  
Commercial Broiler ◽  
Effect Of Feeding

Background: The productivity and profitability of commercial broiler largely depend on nutrition and growth performance and, therefore, this industry requires high quality protein sources, such as Corn Gluten meal (CGM). Many studies have been conducted on protein quality changes and other nutritional characteristics of CGM in several countries Corn gluten meal contains around 60% CP and is a byproduct of wet milling of corn, most of which is used for manufacture of high fructose corn syrup and corn flour. Being high in crude protein, it is often compared to animal protein ingredients during feed formulation. Thus, this study was intended to evaluate the effect of feeding corn gluten meal in feed ration on growth performance of commercial broiler chicken. Methods: A feeding trial was conducted in the year 2018 with an objective to assess the effect of feeding corn gluten meals (CGM) in broiler chicken. Six hundred broiler chickens (day old) were randomly allotted to three groups, comprising of fifty broiler chickens in each treatment group with four replicates. The group-I (T1) Broiler chicken were fed with composite diet containing 5% Corn Gluten meal where as Group-II (T2) received composite diets comprising of CGM 10%. The Group-III (T3) served as control group with normal broiler diet. The experimental period lasted for six weeks. Weekly body weight and feed consumption were measured in each group. Result: Diet composite of 10% of corn gluten meal had significantly (P≤0.05) higher weight gain, lower feed intake and improved FCR than T1 and T3 group. CGM supplementation can be ideally improved broiler weight gain, feed consumption and FCR.

scholarly journals Lethal pneumonia in guineapigs associated with a virus

1981 ◽  
Vol 15 (3) ◽  
pp. 235-242 ◽  
Author(s):  
Susanne Naumann ◽  
I. Kunstýř ◽  
Ina Langer ◽  
J. Maess ◽  
R. Hörning
Keyword(s):  
Epithelial Cells ◽  
High Mortality ◽  
Inclusion Bodies ◽  
Adenovirus Infection ◽  
Microscopical Examination ◽  
Histological Findings ◽  
Intranuclear Inclusion ◽  
Bronchial Epithelial ◽  
Virus Particles ◽  
Electron Microscopical

No bacteria were observed in an epizootic of lethal pneumonia in guineapigs. Necrotic bronchitis and bronchiolitis with basophilic intranuclear inclusion bodies in bronchial epithelial cells were characteristic. Although adenovirus infection of guineapigs has not previously been reported, histological findings paralleled those found in adenovirus infections of other animals including man. Virus particles found by electron-microscopical examination of the lung tissue closely resembled adenoviruses. The disease seemed to have a low contagiousness, a low morbidity (about 0·7%), but an acute course and a high mortality (100%).

Sequential Pathological Study of Experimental Porcine Adenovirus Enteritis

1982 ◽  
Vol 19 (2) ◽  
pp. 179-189 ◽  
Author(s):  
R. Ducatelle ◽  
W. Coussement ◽  
J. Hoorens
Keyword(s):  
Epithelial Cells ◽  
Inclusion Bodies ◽  
Viral Antigen ◽  
Diagnostic Technique ◽  
Immunoperoxidase Technique ◽  
Pathological Study ◽  
Intranuclear Inclusion ◽  
Transmission Electron ◽  
Viral Particles ◽  
Porcine Adenovirus

The sequential enteric lesions of experimental porcine adenovirus strain 6618 infection were studied in 18 hysterotomy-derived, colostrum-deprived piglets, by histology, transmission electron microscopy, and an immunoperoxidase technique. Viral particles could be seen in altered epithelial cells of the lower small intestine from 24 hours until 16 days after inoculation. With the immunoperoxidase technique, viral antigen could be found in epithelial cells at 45 days after challenge. Destruction of epithelial cells and shortening of villi could be related directly to the presence of the virus. Histology of the lower jejunum and ileum, which demonstrates intranuclear inclusion bodies, is proposed as a useful diagnostic technique.

Immunohistopathology of Calf Pneumonia Induced by Endobronchial Inoculation with Bovine Adenovirus 3

2002 ◽  
Vol 39 (5) ◽  
pp. 565-571 ◽  
Author(s):  
M. Narita ◽  
M. Yamada ◽  
T. Tsuboi ◽  
K. Kawashima
Keyword(s):  
Epithelial Cells ◽  
Inclusion Bodies ◽  
System Development ◽  
Inflammatory Cells ◽  
Intranuclear Inclusion ◽  
Caudal Lobe ◽  
Bovine Adenovirus ◽  
Immune System Development ◽  
The Difference ◽  
The Right

Three 1-week-old and three 3-month-old Holstein calves that had received colostrum were inoculated endobronchially with bovine adenovirus 3 (BAV-3). The gross and histologic lesions in these six infected calves were localized mainly in the right caudal lobe of the lung and were closely associated with the site of the deposition of the inoculum. The pneumonic lesions were severe necrotizing bronchitis, bronchiolitis, and alveolitis, accompanied by infiltration of inflammatory cells and proliferation of type 2 pneumocytes. Intranuclear inclusion bodies, BAV-3 antigen, and virus particles were detected in the degenerated epithelial cells in the 1-week-old but not the 3-month-old calves. After infection, the total cell count in the bronchoalveolar lavage (BAL) fluid cells was increased. The results of BAV-3 isolation from BAL fluid were correlated with the detection of intranuclear inclusion bodies in the desquamated epithelial cells in the BAL fluid cells from the right caudal lobe but not in cells from the left caudal lobe. CD8+ T lymphocytes in the pneumonic lesion were found only in the 3-month-old infected calves. The difference in the immunopathologic reactions between the 1-week-old and the 3-month-old infected calves may be attributed to differences in immune system development.

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