scholarly journals Rejuvenated Vintage Tissue Sections Highlight Individual Antigen Fate During Processing and Long-term Storage

2021 ◽  
pp. 002215542110472
Author(s):  
Francesco Mascadri ◽  
Maddalena M. Bolognesi ◽  
Daniela Pilla ◽  
Giorgio Cattoretti
Keyword(s):  
Protein Conformation ◽  
Storage Time ◽  
Antigen Retrieval ◽  
Prolonged Storage ◽  
Tissue Sections ◽  
Long Term Storage ◽  
Calcium Chelation ◽  
Dependent Protein ◽  
Term Storage

Antigen-bearing proteins become progressively unavailable to immunodetection after prolonged storage of routine sections, exposed to a variety of agents, such as moisture, oxygen, and temperature. By proteomic analysis, the antigens are retained in the sections and definitely in the tissue block, pointing to fixation-independent, storage time–dependent protein modifications. Based on previous experience, we hypothesized that a combined exposure to a reducing agent and to chemicals favoring protein conformation changes would reverse the masking in aged sections. Disaccharides, lactose and sucrose, and a surfactant, added to a standard antigen retrieval buffer, reverse the negative changes in aged sections. Furthermore, they provide enhanced access to antigens in freshly cut sections, but not universally, revealing additional factors, besides heat and calcium chelation, required for antigen retrieval of individual proteins:

scholarly journals 3′ MACE RNA-sequencing allows for transcriptome profiling in human tissue samples after long-term storage

2020 ◽  
Vol 100 (10) ◽  
pp. 1345-1355 ◽  
Author(s):  
Stefaniya Boneva ◽  
Anja Schlecht ◽  
Daniel Böhringer ◽  
Hans Mittelviefhaus ◽  
Thomas Reinhard ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Storage Time ◽  
Transcriptome Profiling ◽  
Rna Seq ◽  
Fresh Tissue ◽  
Long Term Storage ◽  
Ffpe Samples ◽  
The Impact ◽  
Term Storage

Abstract This study aims to compare the potential of standard RNA-sequencing (RNA-Seq) and 3′ massive analysis of c-DNA ends (MACE) RNA-sequencing for the analysis of fresh tissue and describes transcriptome profiling of formalin-fixed paraffin-embedded (FFPE) archival human samples by MACE. To compare MACE to standard RNA-Seq on fresh tissue, four healthy conjunctiva from four subjects were collected during vitreoretinal surgery, halved and immediately transferred to RNA lysis buffer without prior fixation and then processed for either standard RNA-Seq or MACE RNA-Seq analysis. To assess the impact of FFPE preparation on MACE, a third part was fixed in formalin and processed for paraffin embedding, and its transcriptional profile was compared with the unfixed specimens analyzed by MACE. To investigate the impact of FFPE storage time on MACE results, 24 FFPE-treated conjunctival samples from 24 patients were analyzed as well. Nineteen thousand six hundred fifty-nine transcribed genes were detected by both MACE and standard RNA-Seq on fresh tissue, while 3251 and 2213 transcripts were identified explicitly by MACE or RNA-Seq, respectively. Standard RNA-Seq tended to yield longer detected transcripts more often than MACE technology despite normalization, indicating that the MACE technology is less susceptible to a length bias. FFPE processing revealed negligible effects on MACE sequencing results. Several quality-control measurements showed that long-term storage in paraffin did not decrease the diversity of MACE libraries. We noted a nonlinear relation between storage time and the number of raw reads with an accelerated decrease within the first 1000 days in paraffin, while the numbers remained relatively stable in older samples. Interestingly, the number of transcribed genes detected was independent on FFPE storage time. RNA of sufficient quality and quantity can be extracted from FFPE samples to obtain comprehensive transcriptome profiling using MACE technology. We thus present MACE as a novel opportunity for utilizing FFPE samples stored in histological archives.

scholarly journals Aboveground storage of hydrocarbon gas hydrates

2018 ◽  
Vol 60 ◽  
pp. 00028
Author(s):  
Mykola Zotsenko ◽  
Larysa Pedchenko ◽  
Andrii Manhura
Keyword(s):  
Gas Hydrate ◽  
Mechanical Characteristics ◽  
Negative Temperature ◽  
Prolonged Storage ◽  
Long Term Storage ◽  
Hydrocarbon Gas ◽  
Mixing Method ◽  
And Storage ◽  
Term Storage

The work objective is to substantiate the technical bases for the production of gas hydrate blocks directly on-site for further transportation and storage in above ground storages, as an alternative to transporting and storing gas in underground gas storages. The theoretical bases have been considered and processes of gas hydrate blocks producing, physical-mechanical characteristics of artificial hydrates with the purpose of determining the conditions of their storage and prolonged storage were tested in laboratory conditions. The construction of above ground gas hydrate storage in the form of a building, partially deepened in a ground, which is separated from the environment by a wall of gravel cement elements made by the mixing method were substantiated. It is proved that such a constructive solution for a long-term storage of gas hydrate blocks is the most economical in terms of energy consumption to maintain internal negative temperature.

scholarly journals Effects of Long-Term Storage Time and Original Sampling Month on Biobank Plasma Protein Concentrations

EBioMedicine ◽  
2016 ◽  
Vol 12 ◽  
pp. 309-314 ◽  
Author(s):  
Stefan Enroth ◽  
Göran Hallmans ◽  
Kjell Grankvist ◽  
Ulf Gyllensten
Keyword(s):  
Plasma Protein ◽  
Storage Time ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Stability of Folate and Vitamin B12in Human Serum after Long-Term Storage: A Follow-Up after 13 Years

2018 ◽  
Vol 2018 ◽  
pp. 1-4 ◽  
Author(s):  
Eugène H. J. M. Jansen ◽  
Piet K. Beekhof
Keyword(s):  
Human Serum ◽  
Storage Time ◽  
Vitamin B ◽  
Serum Samples ◽  
Long Term Storage ◽  
Nutrition Research ◽  
The Stability ◽  
Term Storage

In epidemiological and nutrition research, it is very important to evaluate the stability of biomarkers as function of both storage time and temperature. In this study, the stability of folate and vitamin B12in human serum samples has been tested after long-term storage at −80°C up to 13 years. Serum samples of 16 individuals were used in this study. The concentration of folate and vitamin B12has been determined att=0and at 1, 8, and 13 years after storage at −80°C. The folate concentrations in serum samples remained stable at −80°C. The concentration of vitamin B12was decreasing during the time of the study to about 50%. The correlation of the folate and also of the vitamin B12concentrations in the stored samples compared with the starting values was still good. Therefore, although the concentration of vitamin B12decreased upon storage, reliable comparative analyses can still be performed.

scholarly journals Features of formation of durability of corn hybrids seeds for their cultivation, postharvest processing and storage

Agrology ◽  
2021 ◽  
Vol 4 (3) ◽  
pp. 137-142
Author(s):  
M. Ya. Kyrpa ◽  
N. S. Filipkova
Keyword(s):  
Seed Production ◽  
Necessary Condition ◽  
Prolonged Storage ◽  
Hydrothermal Conditions ◽  
Corn Hybrids ◽  
Long Term Storage ◽  
Processing And Storage ◽  
And Storage ◽  
Term Storage

The organization of the sowing material stocks is a necessary condition for stable seed production of any crop. Insurance, reserve, breeding and transi-tional funds are such stocks in corn seed production. Their creation and maintenance largely depend on the economic longev-ity of seeds, which can be formed at the stages of cultivation, harvesting, post-harvest processing and storage of seed. However, the data on the peculiarities of the formation of seed longevity of corn hybrids and their ability to long-term storage are lim-ited. That is why research in this area is considered relevant and important. The results of study of regularities of formation and factors of longevity of corn hybrids seeds of domestic selection are summarized in the executed work. Ecological, technical-technological and varietal factors are formed dur-ing corn cultivation, har-vesting and processing. The greatest dependence is noted on the hydrothermal conditions, start-ing from the stage of cultivation, and then – in the stages after harvesting - the modes of drying and separation of seed. High economic durability is provided under the following conditions: hydro-term coefficient at the level of 0,5–0,7 – for vegetation and 0,3–0,4 – for maturing, dry-ing temperature within 36–400С at humidity of seeds of 20–30%, fractional separation, removal from the seeds mass of the unstable frac-tion containing 18–20% of the total. Some of the factors included a sign of seed size, which is clearly manifested at the stage of long-term storage of seed. Sowing fractions differ significantly in terms of size, including the weight of 1000 seeds and their linear size. The greatest economic durability is inherent in the first and second of all factions. It is recommended to obtain them by calibrating the seeds on separating sieves with round holes with a diameter of 8–10 mm, depending on the hybrids. Prolonged storage is proposed on the basis of a combination of two factors – low humidity and airtight packaging of seeds. This significantly reduces resource costs and extends the economic longevity of corn hybrid seeds to 4–5 years.

Viability and Engraftment of Hematopoietic Progenitor Cells (HPC) After Long Term Cryopreservation: Effect of Diagnosis and % Dimethylsulfoxide (DMSO) Concentration.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 3161-3161
Author(s):  
Muthu Kumaran Veeraputhiran ◽  
John Theus ◽  
Gina Pesek ◽  
Bart Barlogie ◽  
Michele H. Cottler-Fox
Keyword(s):  
Storage Time ◽  
Conflicts Of Interest ◽  
Population Data ◽  
T Test ◽  
Hematopoietic Progenitor ◽  
Long Term Storage ◽  
Significant Difference ◽  
Dmso Concentration ◽  
Term Storage

Abstract Abstract 3161 Poster Board III-98 Introduction There are few reports of HPC viability after more than 9 y of storage. It has been suggested that HPC from myeloma patients (pts) may survive storage less well than those from other diseases. The appropriate concentration of DMSO for long term storage is unclear. Our program has collected and stored HPC since November 1989, changing from 5% DMSO to 10% DMSO in August 2000. We present a retrospective analysis of viability by diagnosis and DMSO concentration in pts who underwent autologous transplant using HPC after long-term storage (up to 17.8 y). Materials and Methods HPC were harvested and preserved using a controlled rate freezer and 5% or 10% DMSO, then stored in liquid nitrogen. Viability was tested by flow cytometry using unwashed samples and looking at propidium iodide or 7-AAD uptake within the CD34+ population. Data from 242 samples were analyzed (224 myeloma pts and 18 other diagnoses): 100 consecutively thawed samples with storage time <1 y (all 10% DMSO), 100 consecutive samples stored from 5-9 y ( 7 samples, 10% and 93 samples, 5% DMSO), and all samples stored and used for transplant after >9y (42 samples, 5% DMSO). No statistically significant difference was seen in viability between <1y and 5-9y, so these groups were combined and compared to >9y. White blood cell (WBC) and platelet engraftment were compared for the 2 groups using an unpaired t test. Results Mean viability for the 0-9y and >9 y groups were 91.62% and 92.79% respectively, with no statistically significant difference in viability between groups (p=0.28). Using a 2-tailed t test, no difference was seen in viability based on diagnosis (p=0.19) or between 5% and 10% DMSO (p= 0.12). No difference was seen in WBC engraftment (p=0.28) or platelet engraftment between groups (p=0.57). Discussion HPC collections remained viable after 17.8 years, the longest time reported for use after storage to date. No difference in viability was seen with 5% or 10% DMSO, or between myeloma and other diseases. Storage time did not affect engraftment. Disclosures No relevant conflicts of interest to declare.

Factors affecting immunoreactivity in long-term storage of formalin-fixed paraffin-embedded tissue sections

2015 ◽  
Vol 144 (1) ◽  
pp. 93-99 ◽  
Author(s):  
Federica Grillo ◽  
Simona Pigozzi ◽  
Paola Ceriolo ◽  
Paola Calamaro ◽  
Roberto Fiocca ◽  
...  
Keyword(s):  
Factors Affecting ◽  
Tissue Sections ◽  
Long Term Storage ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Term Storage ◽  
Formalin Fixed

scholarly journals 1-Methylcyclopropene postharvest treatment and their effect on apple quality during long-term storage time

2014 ◽  
Vol 239 (4) ◽  
pp. 603-612 ◽  
Author(s):  
Joanna Kolniak-Ostek ◽  
Aneta Wojdyło ◽  
Jarosław Markowski ◽  
Karolina Siucińska
Keyword(s):  
Storage Time ◽  
Long Term Storage ◽  
Postharvest Treatment ◽  
Apple Quality ◽  
Term Storage

scholarly journals Germination of Hymenaea courbaril: Storage Behavior, Origin, and Substrate

2020 ◽  
Author(s):  
Leonardo Regnier
Keyword(s):  
Storage Period ◽  
Significant Loss ◽  
Prolonged Storage ◽  
Hymenaea Courbaril ◽  
Long Term Storage ◽  
Germination Potential ◽  
Population Origin ◽  
One Year ◽  
Term Storage

Hymenaea courbaril is an endangered species, promising to reforestation programs and mainly explored as a wood source. The available information concerning long-term storage methods, seed recalcitrance, parental, and substrate influence is scarce. This study focused on the seed behavior according to population origin and during one-year storage, also testing the efficiency of the low-temperature conservation. Variations between the uncertainty indexes were found to the studied populations after long-term storage. There was no significant loss of the germination potential in consequence of the prolonged storage period. Although, older seeds promoted gradually greater delayed germination. Germination speed, synchrony, and uncertainty indexes were substantially different between the -20&deg; conservation and control. H. courbaril seeds are capable of long-term storage without losing their germination potential, indicating an orthodox behavior.

scholarly journals Quality of ‘Hayward’ Kiwifruit in Prolonged Cold Storage as Affected by the Stage of Maturity at Harvest

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 358
Author(s):  
Tal Goldberg ◽  
Harel Agra ◽  
Ruth Ben-Arie
Keyword(s):  
Fruit Quality ◽  
Titratable Acidity ◽  
Soluble Solids ◽  
Prolonged Storage ◽  
Full Bloom ◽  
Long Term Storage ◽  
Solids Content ◽  
Term Storage

The effect of ‘Hayward’ kiwifruit maturity at harvest on fruit quality during long-term storage at −0.5 °C was evaluated by harvesting the fruit several times, at different stages of maturity. The progress of maturation on the vine was monitored weekly from 136 DAFB (days after full bloom). Fruit were harvested for storage at three points and stored for 3–6 months in regular air (RA), or for 6–10 months in a controlled atmosphere (CA), with or without prestorage exposure to 1-methylcyclopropene (1-MCP). The softening rate under both storage regimes decreased with the advance in fruit maturation on the vine, as indicated by increasing soluble solids content (SSC), and declining firmness. As a result, the fruit from the first harvest (152 DAFB), which were the firmest at harvest, were the softest at the end of both storage regimes. Delaying harvest also decelerated the decline in acidity during storage, so that fruit picked last maintained the highest titratable acidity (TA) upon removal from storage. The overall fruit quality after shelf life, following prolonged storage in either RA or CA, was improved by delaying harvest to late November (ca. 200 DAFB). The harvest criteria for fruit with the best storage potential were dry matter (DM) > 17%, SSC > 7%, TA 2.0–2.6%, with more than 40% of the DM non soluble. From a commercial aspect the rule should therefore be ‘Last in, last out’ (LILO).

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