scholarly journals The Stem Cell Marker CD133 (Prominin-1) Is Expressed in Various Human Glandular Epithelia

2008 ◽  
Vol 56 (11) ◽  
pp. 977-993 ◽  
Author(s):  
Jana Karbanová ◽  
Ewa Missol-Kolka ◽  
Ana-Violeta Fonseca ◽  
Christoph Lorra ◽  
Peggy Janich ◽  
...  
Keyword(s):  
Bile Ducts ◽  
Stem Cell Marker ◽  
Sweat Glands ◽  
Mucous Cells ◽  
Cell Marker ◽  
Apocrine Glands ◽  
Adult Human ◽  
Apical Domain ◽  
Eccrine Glands ◽  
Stem And Progenitor Cells

Human prominin-1 (CD133) is expressed by various stem and progenitor cells originating from diverse sources. In addition to stem cells, its mouse ortholog is expressed in a broad range of adult epithelial cells, where it is selectively concentrated in their apical domain. The lack of detection of prominin-1 in adult human epithelia might be explained, at least in part, by the specificity of the widely used AC133 antibody, which recognizes an epitope that seems dependent on glycosylation. Here we decided to re-examine its expression in adult human tissues, particularly in glandular epithelia, using a novel monoclonal antibody (80B258) generated against the human prominin-1 polypeptide. In examined tissues, we observed 80B258 immunoreactivity at the apical or apicolateral membranes of polarized cells. For instance, we found expression in secretory serous and mucous cells as well as intercalated ducts of the large salivary and lacrimal glands. In sweat glands including the gland of Moll, 80B258 immunoreactivity was found in the secretory (eccrine and apocrine glands) and duct (eccrine glands) portion. In the liver, 80B258 immunoreactivity was identified in the canals of Hering, bile ductules, and small interlobular bile ducts. In the uterus, we detected 80B258 immunoreactivity in endometrial and cervical glands. Together these data show that the overall expression of human prominin-1 is beyond the rare primitive cells, and it seems to be a general marker of apical or apicolateral membrane of glandular epithelia. This manuscript contains online supplemental material at http://www.jhc.org . Please visit this article online to view these materials.

scholarly journals Comparative Study of the Composition of Sweat from Eccrine and Apocrine Sweat Glands during Exercise and in Heat

2020 ◽  
Vol 17 (10) ◽  
pp. 3377 ◽  
Author(s):  
Yi-Lang Chen ◽  
Wen-Hui Kuan ◽  
Chao-Lin Liu
Keyword(s):  
Uric Acid ◽  
Metabolic Rate ◽  
Repeated Measures ◽  
Dynamic Exercise ◽  
Sweat Glands ◽  
Apocrine Glands ◽  
Young Males ◽  
Repeated Measures Analysis ◽  
Eccrine Glands ◽  
Excretion Level

This preliminarily study was made to examine the differences in sweat excretions from human eccrine and apocrine sweat glands in dynamic exercise and heat conditions. Sweat samples were collected from six young males while they were either running on a treadmill or sitting in a sauna cabinet. Sweat samples of at least 5 mL from the eccrine (upper−back) and apocrine (armpit) sweat glands were collected during a 20−min running (or inactive overheating) period. The samples were then analyzed for urea, uric acid, and electrolyte (Na+, Cl−, and K+) excretions. The results from a two−way repeated−measures analysis of variance (ANOVA) revealed that the secretions of urea and K+ were significantly higher during running than during inactive overheating for both glands, as were Na+ secretions for the apocrine glands (all P < 0.05). Under the same sweating conditions, urea and K+ excretions from the apocrine glands were also higher than those from the eccrine glands (all P < 0.05). Significant differences were observed between the Na+ secretions of the apocrine and eccrine glands under the running condition. The effects of various sweating methods and sweat glands on Cl− secretions were nonsignificant, and little uric acid was excreted. A higher urea excretion level during running rather than in hot conditions could be attributed to an elevated metabolic rate.

scholarly journals Pluripotency-associated stem cell marker expression in proliferative cell cultures derived from adult human pancreas

2011 ◽  
Vol 211 (2) ◽  
pp. 169-176 ◽  
Author(s):  
Michael G White ◽  
Hussain R Al-Turaifi ◽  
Graham N Holliman ◽  
Ali Aldibbiat ◽  
Aiman Mahmoud ◽  
...  
Keyword(s):  
Stem Cell ◽  
Western Blot ◽  
Gradient Centrifugation ◽  
Stem Cell Marker ◽  
Stem Cell Markers ◽  
Human Pancreas ◽  
Cell Marker ◽  
Β Cells ◽  
Pancreatic Cell ◽  
Adult Human

The source of new β-cells in adult human pancreas remains incompletely elucidated with recent studies on rodents providing evidence for neogenesis from progenitor cells in addition to self-replication. The aim of this study was to investigate the expression of pluripotency-associated stem cell markers in proliferative cultures derived from adult human pancreas. Human pancreatic tissue was obtained from deceased donors following ethical approval and relative consent. Islet-enriched fraction was separated from the retrieved organ by digestion and density gradient centrifugation. Dissociated cells were seeded in adherent culture forming proliferative ‘islet survivor cells’ (ISCs). These were characterised at fifth passage by RT-PCR, immunofluorescence staining, FACS, western blot and transfection studies with an OCT4 promoter-driven reporter. Nuclear expression of the pluripotency-associated stem cell marker complex OCT4/SOX2/NANOG was confirmed in ISCs. The phenotype constituted ∼8% of the overall population. OCT4 biosynthesis was confirmed by western blot and activation of an exogenous OCT4 promoter. Co-expression of pluripotency-associated markers has been confirmed in proliferative primary cells derived from adult human pancreas. Further studies are required to elucidate whether these cells possess functional stem cell characteristics and assess potential for differentiation into pancreatic cell lineages including new β-cells.

Prominin-1/CD133, a neural and hematopoietic stem cell marker, is expressed in adult human differentiated cells and certain types of kidney cancer

2004 ◽  
Vol 319 (1) ◽  
pp. 15-26 ◽  
Author(s):  
Mareike Florek ◽  
Michael Haase ◽  
Anne-Marie Marzesco ◽  
Daniel Freund ◽  
Gerhard Ehninger ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Kidney Cancer ◽  
Stem Cell Marker ◽  
Cell Marker ◽  
Hematopoietic Stem ◽  
Adult Human ◽  
Differentiated Cells

Stem Cell Marker Prominin-1/AC133 Is Expressed in Duct Cells of the Adult Human Pancreas

Pancreas ◽  
2008 ◽  
Vol 36 (1) ◽  
pp. e1-e6 ◽  
Author(s):  
Jessy Lardon ◽  
Denis Corbeil ◽  
Wieland B. Huttner ◽  
Zhidong Ling ◽  
Luc Bouwens
Keyword(s):  
Stem Cell ◽  
Stem Cell Marker ◽  
Human Pancreas ◽  
Cell Marker ◽  
Adult Human ◽  
Duct Cells

scholarly journals A New Case of Syringocystadenocarcinoma Papilliferum: A Rare Pathology for a Wide-Ranging Comprehension

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Beatrice Paradiso ◽  
Enzo Bianchini ◽  
Pierangelo Cifelli ◽  
Luigi Cavazzini ◽  
Giovanni Lanza
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Pluripotent Stem Cells ◽  
Stem Cell Marker ◽  
Cell Marker ◽  
Cytokeratin 7 ◽  
Apocrine Glands ◽  
Rare Neoplasm ◽  
Ultrastructural Studies ◽  
Syringocystadenoma Papilliferum

We report a new case of p63/cytokeratin 7 (CK7) positive syringocystadenocarcinoma papilliferum (SCACP), on the shoulder of an 88-year-old man, with superficial dermal infiltration and squamoid differentiation. We describe the 24th case of SCACP, the malignant counterpart of syringocystadenoma papilliferum (SCAP). At the present, we do not know whether SCACP arises from eccrine or apocrine glands because of the contrasting opinions in the literature. Only few histochemical and ultrastructural studies have previously advised that SCACP could arise from pluripotent stem cells. Through our case, we wish to suggest the stem cell-like properties of the syringocystadenocarcinoma papilliferum. This rare neoplasm shows two different patterns of stem cell marker expression in the glandular and squamous components, respectively. For the double phenotype of SCACP, we propose it like an intriguing model to study histogenesis and stem cell properties for more wide-ranging epithelial tumors.

scholarly journals Midbody and primary cilium of neural progenitors release extracellular membrane particles enriched in the stem cell marker prominin-1

2007 ◽  
Vol 176 (4) ◽  
pp. 483-495 ◽  
Author(s):  
Véronique Dubreuil ◽  
Anne-Marie Marzesco ◽  
Denis Corbeil ◽  
Wieland B. Huttner ◽  
Michaela Wilsch-Bräuninger
Keyword(s):  
Stem Cell ◽  
Progenitor Cells ◽  
Primary Cilium ◽  
Neural Progenitors ◽  
Stem Cell Marker ◽  
Cell Marker ◽  
Membrane Particles ◽  
Membrane Microdomain ◽  
Neuroepithelial Cells ◽  
Stem And Progenitor Cells

Expansion of the neocortex requires symmetric divisions of neuroepithelial cells, the primary progenitor cells of the developing mammalian central nervous system. Symmetrically dividing neuroepithelial cells are known to form a midbody at their apical (rather than lateral) surface. We show that apical midbodies of neuroepithelial cells concentrate prominin-1 (CD133), a somatic stem cell marker and defining constituent of a specific plasma membrane microdomain. Moreover, these apical midbodies are released, as a whole or in part, into the extracellular space, yielding the prominin-1–enriched membrane particles found in the neural tube fluid. The primary cilium of neuroepithelial cells also concentrates prominin-1 and appears to be a second source of the prominin-1–bearing extracellular membrane particles. Our data reveal novel origins of extracellular membrane traffic that enable neural stem and progenitor cells to avoid the asymmetric inheritance of the midbody observed for other cells and, by releasing a stem cell membrane microdomain, to potentially influence the balance of their proliferation versus differentiation.

scholarly journals Newer Insights Into Premeiotic Development of Germ Cells in Adult Human Testis Using Oct-4 as a Stem Cell Marker

2010 ◽  
Vol 58 (12) ◽  
pp. 1093-1106 ◽  
Author(s):  
Deepa Bhartiya ◽  
Sandhya Kasiviswanathan ◽  
Sreepoorna K. Unni ◽  
Prasad Pethe ◽  
Jayesh V. Dhabalia ◽  
...  
Keyword(s):  
Stem Cell ◽  
Germ Cells ◽  
Stem Cell Marker ◽  
Human Testis ◽  
Cell Marker ◽  
Adult Human

Morphology and development of an apoeccrine sweat gland in human axillae

1987 ◽  
Vol 252 (1) ◽  
pp. R166-R180 ◽  
Author(s):  
K. Sato ◽  
R. Leidal ◽  
F. Sato
Keyword(s):  
Electron Microscopy ◽  
Hair Follicle ◽  
Sweat Gland ◽  
Sweat Glands ◽  
Apocrine Gland ◽  
Dark Cells ◽  
Adult Human ◽  
Data Support ◽  
Eccrine Glands ◽  
Segmental Dilatation

Evidence is presented that in adult human axillae there exists a third type of sweat gland tentatively designated as the apoeccrine sweat gland. This type of gland shows a segmental or diffuse apocrinelike dilatation of its secretory tubule but has a long and thin duct which does not open into a hair follicle. The electron microscopy of its dilated segment is often indistinguishable from that of the classical apocrine gland. The less remarkably dilated segment of the apoeccrine gland tends to retain intercellular canaliculi and/or dark cells. These apoeccrine glands are consistently present in adult human axillae regardless of sex or race. In the axillae of the two 6-yr-old subjects, both classical apocrine and eccrine glands were present but no apoeccrine glands were found. Between 8–14 yr of age, the number of large eccrine glands with or without partial segmental dilatation gradually increased. At 16–18 yr of age, the number of apoeccrine glands increased to as high as 45% of the total axillary glands. The data support the notion that apoeccrine glands develop during puberty in the axillae from eccrine or eccrinelike sweat glands.

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