scholarly journals Correction: Diagnostic Accuracy of Real-Time PCR Assays Targeting 16S rRNA and lipl32 Genes for Human Leptospirosis in Thailand: A Case-Control Study

PLoS ONE ◽  
2011 ◽  
Vol 6 (2) ◽  
Author(s):  
Janjira Thaipadungpanit ◽  
Wirongrong Chierakul ◽  
Vanaporn Wuthiekanun ◽  
Direk Limmathurotsakul ◽  
Premjit Amornchai ◽  
...  
Keyword(s):  
16S Rrna ◽  
Diagnostic Accuracy ◽  
Real Time ◽  
Real Time Pcr ◽  
Case Control Study ◽  
Case Control ◽  
Human Leptospirosis ◽  
Pcr Assays ◽  
Control Study

scholarly journals Diagnostic Accuracy of Real-Time PCR Assays Targeting 16S rRNA and lipl32 Genes for Human Leptospirosis in Thailand: A Case-Control Study

PLoS ONE ◽  
2011 ◽  
Vol 6 (1) ◽  
pp. e16236 ◽  
Author(s):  
Janjira Thaipadunpanit ◽  
Wirongrong Chierakul ◽  
Vanaporn Wuthiekanun ◽  
Direk Limmathurotsakul ◽  
Premjit Amornchai ◽  
...  
Keyword(s):  
16S Rrna ◽  
Diagnostic Accuracy ◽  
Real Time ◽  
Real Time Pcr ◽  
Case Control Study ◽  
Case Control ◽  
Human Leptospirosis ◽  
Pcr Assays ◽  
Control Study

scholarly journals The association of male infertility with telomere length: A case control study

2021 ◽  
Vol 8 (4) ◽  
pp. 325-332
Author(s):  
Kate Deepali Rajesh ◽  
Puranam Vatsalaswamy ◽  
Manvikar Purshotam Rao
Keyword(s):  
Male Infertility ◽  
Telomere Length ◽  
Real Time ◽  
Real Time Pcr ◽  
Case Control Study ◽  
Case Control ◽  
Control Group ◽  
Significant Difference ◽  
Control Study ◽  
Sperm Telomere Length

To study the relevance of sperm telomere length and infertility in men. : Our case-control study included twenty-five males in couple with sub-fertility/infertility (test group) and twenty five healthy males (control group) with proven paternity in the age group 25 to 35 years. The Absolute Sperm Telomere length (aSTL) was measured by real-time PCR. We investigated whether any significant difference in the aSTL value existed between the groups and analysed the relationship between aSTL and other sperm parameters.The mean (SE) aSTL recorded in the infertile cases was significantly shorter than for the control group being 140.60 (6.66) Kb/genome and 239.63 (12.32) Kb/genome respectively (p <0.001) A weak correlation was eminent between aSTL kb/genome and the total sperm count mil/ml (rho= 0.04, p - 0.86), progressive sperm motility (rho= - 0.02, p=0.934) and sperm viability (rho= - 0.07 p=0.741) in the infertile group. The measurement of aSTL by real-time PCR is a simple and rapid method that offers further paramount information with respective to the quality of sperm. It is befitted for epidemiological studies, hence opening new perspectives in the evaluation of male infertility. Limitations - Our study was confined to men aged between 25 and 35 years. Further comparative studies are needed to explore the significance of STL and infertility in older males. Additional studies will help illumine the significance of aSTL as a prognostic biomarker in assisted reproduction.

scholarly journals Type specific Real time PCR for detection of human herpes virus 6 in schizophrenia and bipolar patients: a case control study

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Jila Yavarian ◽  
Somaye Shatizadeh Malekshahi ◽  
Roya Yavarian ◽  
Shaghayegh Yazdani ◽  
Leila Janani ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Case Control Study ◽  
Case Control ◽  
Human Herpes ◽  
Human Herpes Virus ◽  
Human Herpes Virus 6 ◽  
Herpès Virus ◽  
Bipolar Patients ◽  
Control Study

scholarly journals Sensitive Biomarker Analysis of Xue-Fu-Zhu-Yu Capsule for Patients with Qi Stagnation and Blood Stasis Pattern: A Nested Case-Control Study

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Guang Chen ◽  
Haoqiang He ◽  
Kun Hu ◽  
Jialiang Gao ◽  
Jun Li ◽  
...  
Keyword(s):  
Treatment Group ◽  
Real Time ◽  
Real Time Pcr ◽  
Case Control Study ◽  
Blood Stasis ◽  
Case Control ◽  
Expression Level ◽  
Qi Stagnation ◽  
Nested Case Control ◽  
Control Study

Objectives. To identify the sensitive biomarker to predict the effectiveness of Xue-Fu-Zhu-Yu capsules (XFZYC). Methods. This nested case-control study included 5 patients with response to XFZYC in the treatment group, 5 patients with no response to XFZYC also in the treatment group, and 5 patients in the control group treated with placebo who participated in the previous RCT. The mRNAs, miRNAs, lncRNAs, and circRNAs were sequenced by next-generation sequencing and differential-expressed (DE) RNAs were identified if p value ≤ 0.05 and fold change ≥2, bioinformatics analysis was conducted in terms of function annotations and signaling pathways, and then sensitive biomarker was analyzed based on real-time PCR. Results. The distributions of clinical characteristics between the selected participants from treatment group and placebo group were well balanced. A total of 1156 DE RNAs, 388 miRNAs, 1954 lncRNAs, and 560 circRNAs were identified, which was associated the mechanism of XFZYC and composed the targeted potential biomarkers for further real-time PCR. The DE RNAs were enriched in KEGG pathways pertaining to pathogenesis of Qi Stagnation and Blood Stasis- (QS & BS-) & associated diseases such as coronary heart disease and digestive diseases. The expression level of FZD8 was significantly higher in response patients than that in nonresponse patients (p=0.041) and circRNA_13799 significantly lower in response patients than that in nonresponse patients (p=0.040) based on real-time PCR. Patients with higher expression level of FZD8 with 75% stratification have significantly higher reduction in the questionnaire score (p=0.010), and the area under the curve (AUC) was 0.765 (95%CI = 0.593–0.936; p=0.014). Conclusions. FZD8 might perform the sensitive biomarker for predicting the effectiveness of XFZYC. However, further prospective cohort study was warranted to confirm the exact specificity and sensitivity of this biomarker.

scholarly journals Diagnostic accuracy and validity of three manual examination tests to identify alar ligament lesions: results of a blinded case-control study

2018 ◽  
Vol 27 (2) ◽  
pp. 83-91 ◽  
Author(s):  
Piekartz Harry Von ◽  
Rakan Maloul ◽  
Marisa Hoffmann ◽  
Toby Hall ◽  
Med Martin Ruch ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Case Control Study ◽  
Case Control ◽  
Alar Ligament ◽  
Manual Examination ◽  
Control Study

scholarly journals A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Frieder Schaumburg ◽  
Neele Froböse ◽  
Robin Köck
Keyword(s):  
Multiplex Pcr ◽  
Case Control Study ◽  
Case Control ◽  
Test Target ◽  
Screening Assay ◽  
E Coli ◽  
Multiplex Pcr Assay ◽  
Pcr Assays ◽  
Traveller’S Diarrhoea ◽  
Control Study

Abstract Background Numerous multiplex-PCR assays are now available in routine diagnostics but their clinical value is controversial if a clear association between clinical symptoms and the detection of a particular pathogen is missing. The objective of this work was to evaluate a multiplex-PCR assay for the diagnosis of traveller’s diarrhoea (TD) in a case-control study and to assess the concordance with the BioFire® FilmArray® Gastrointestinal Panel. Methods Stool samples from cases (n = 61) and controls (n = 30) were collected during travel and analysed by the GI-EB Screening assay (Seegene) in a case-control study. The concordance with the BioFire® FilmArray® Gastrointestinal Panel was expressed as the proportion of participants in which both tests agreed in the category “detected” and “not detected”. Results None of the test-target organisms (Campylobacter spp., Clostridioides difficile toxin A/B, Salmonella spp., Shigella spp./enteroinvasive Escherichia coli, E. coli O157, Shiga toxin-producing E. coli, Yersinia enterocolitica) was significantly associated with TD GI-EB Screening assay. The GI-EB Screening assay had an agreement with the BioFire® FilmArray® of 86.8–100%. Conclusion The selection of test-target organisms included in the GI-EB Screening assay appears inappropriate for the diagnostic work-up of TD as none of the detected pathogens was associated with TD. The GI-EB Screening assay had a good concordance with BioFire® FilmArray®.

scholarly journals Accuracy of a Self-monitoring Test for Identification and Monitoring of Age-related Macular Degeneration: A Diagnostic Case-control Study

2018 ◽  
Vol 12 (1) ◽  
pp. 19-28 ◽  
Author(s):  
Martin K. Schmid ◽  
Livia Faes ◽  
Lucas M. Bachmann ◽  
Michael A. Thiel
Keyword(s):  
Diagnostic Accuracy ◽  
Case Control Study ◽  
Case Control ◽  
Age Related Macular Degeneration ◽  
Index Test ◽  
Vernier Acuity ◽  
Self Monitoring ◽  
Age Related ◽  
Control Study ◽  
Wet Amd

Objective: To evaluate diagnostic accuracy of a new self-monitoring device using a Vernier hyperacuity alignment task. Method: A total of 11 wet Age-Related Macular Degeneration (AMD) patients and 9 controls contributing 37 eyes were consecutively enrolled into this prospective diagnostic case-control study at the retina centre of the Cantonal Hospital Lucerne, Switzerland. Vernier acuity testing (index test) and Optical Coherence Tomography (OCT, reference test) were performed in all participants. OCT scans were evaluated and graded by a retinal specialist masked to diagnosis and index test results. Candidate parameters of the index test to be used as the diagnostic statistic were identified using a bootstrap procedure. Ten parameters remaining were further assessed in univariate analyses. The overall Standard Deviation (SD) of absolute distances across all four axes of the Vernier acuity test provided the highest area under the Receiver Operating Characteristics (ROC) curve and was therefore selected. Results: Mean age of patients with wet AMD was 81.2 years (SD 4.99), mean numbers of letters were 67.4 (SD 14.1). The proportion of women was similar in both groups (controls: 88%, wet AMD: 72%). The area under the ROC curve was 0.87 (95% confidence interval CI: 0.75- 0.99) indicating excellent discrimination. Best accuracy was reached at a cut-off value of 0.64 with a sensitivity of 75% and a specificity of 94%. Conclusion: This diagnostic case-control study of a new screening device for AMD shows acceptable diagnostic accuracy. The promising preliminary data of this study call for further upstream evaluations in reasonably sized clinical studies.

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