Type specific Real time PCR for detection of human herpes virus 6 in schizophrenia and bipolar patients: a case control study

To study the relevance of sperm telomere length and infertility in men. : Our case-control study included twenty-five males in couple with sub-fertility/infertility (test group) and twenty five healthy males (control group) with proven paternity in the age group 25 to 35 years. The Absolute Sperm Telomere length (aSTL) was measured by real-time PCR. We investigated whether any significant difference in the aSTL value existed between the groups and analysed the relationship between aSTL and other sperm parameters.The mean (SE) aSTL recorded in the infertile cases was significantly shorter than for the control group being 140.60 (6.66) Kb/genome and 239.63 (12.32) Kb/genome respectively (p <0.001) A weak correlation was eminent between aSTL kb/genome and the total sperm count mil/ml (rho= 0.04, p - 0.86), progressive sperm motility (rho= - 0.02, p=0.934) and sperm viability (rho= - 0.07 p=0.741) in the infertile group. The measurement of aSTL by real-time PCR is a simple and rapid method that offers further paramount information with respective to the quality of sperm. It is befitted for epidemiological studies, hence opening new perspectives in the evaluation of male infertility. Limitations - Our study was confined to men aged between 25 and 35 years. Further comparative studies are needed to explore the significance of STL and infertility in older males. Additional studies will help illumine the significance of aSTL as a prognostic biomarker in assisted reproduction.

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Use of multiplex PCR and real-time PCR to detect human herpes virus genome in ocular fluids of patients with uveitis

British Journal of Ophthalmology ◽

10.1136/bjo.2007.133967 ◽

2008 ◽

Vol 92 (7) ◽

pp. 928-932 ◽

Cited By ~ 108

Author(s):

S Sugita ◽

N Shimizu ◽

K Watanabe ◽

M Mizukami ◽

T Morio ◽

...

Keyword(s):

Multiplex Pcr ◽

Real Time ◽

Real Time Pcr ◽

Herpes Virus ◽

Virus Genome ◽

Human Herpes ◽

Human Herpes Virus ◽

Ocular Fluids ◽

Herpès Virus

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Sensitive Biomarker Analysis of Xue-Fu-Zhu-Yu Capsule for Patients with Qi Stagnation and Blood Stasis Pattern: A Nested Case-Control Study

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2019/7182865 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12

Author(s):

Guang Chen ◽

Haoqiang He ◽

Kun Hu ◽

Jialiang Gao ◽

Jun Li ◽

...

Keyword(s):

Treatment Group ◽

Real Time ◽

Real Time Pcr ◽

Case Control Study ◽

Blood Stasis ◽

Case Control ◽

Expression Level ◽

Qi Stagnation ◽

Nested Case Control ◽

Control Study

Objectives. To identify the sensitive biomarker to predict the effectiveness of Xue-Fu-Zhu-Yu capsules (XFZYC). Methods. This nested case-control study included 5 patients with response to XFZYC in the treatment group, 5 patients with no response to XFZYC also in the treatment group, and 5 patients in the control group treated with placebo who participated in the previous RCT. The mRNAs, miRNAs, lncRNAs, and circRNAs were sequenced by next-generation sequencing and differential-expressed (DE) RNAs were identified if p value ≤ 0.05 and fold change ≥2, bioinformatics analysis was conducted in terms of function annotations and signaling pathways, and then sensitive biomarker was analyzed based on real-time PCR. Results. The distributions of clinical characteristics between the selected participants from treatment group and placebo group were well balanced. A total of 1156 DE RNAs, 388 miRNAs, 1954 lncRNAs, and 560 circRNAs were identified, which was associated the mechanism of XFZYC and composed the targeted potential biomarkers for further real-time PCR. The DE RNAs were enriched in KEGG pathways pertaining to pathogenesis of Qi Stagnation and Blood Stasis- (QS & BS-) & associated diseases such as coronary heart disease and digestive diseases. The expression level of FZD8 was significantly higher in response patients than that in nonresponse patients (p=0.041) and circRNA_13799 significantly lower in response patients than that in nonresponse patients (p=0.040) based on real-time PCR. Patients with higher expression level of FZD8 with 75% stratification have significantly higher reduction in the questionnaire score (p=0.010), and the area under the curve (AUC) was 0.765 (95%CI = 0.593–0.936; p=0.014). Conclusions. FZD8 might perform the sensitive biomarker for predicting the effectiveness of XFZYC. However, further prospective cohort study was warranted to confirm the exact specificity and sensitivity of this biomarker.

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