scholarly journals Genetic variation in Interleukin-32 influence the immune response against New World Leishmania species and susceptibility to American Tegumentary Leishmaniasis

2020 ◽  
Vol 14 (2) ◽  
pp. e0008029 ◽  
Author(s):  
Jéssica Cristina dos Santos ◽  
Valéria Bernadete Leite Quixabeira ◽  
Muriel Vilela Teodoro Silva ◽  
Michelle S. M. A. Damen ◽  
Kiki Schraa ◽  
...  
Keyword(s):  
Immune Response ◽  
Genetic Variation ◽  
New World ◽  
Leishmania Species ◽  
Tegumentary Leishmaniasis ◽  
American Tegumentary Leishmaniasis

scholarly journals Cellular immune response profile in patients with American tegumentary leishmaniasis prior and post chemotherapy treatment

2009 ◽  
Vol 23 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Luiza C. Reis ◽  
Maria Edilenza F. Brito ◽  
Marina A. Souza ◽  
Angela C.R. Medeiros ◽  
Claudio J. Silva ◽  
...  
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Chemotherapy Treatment ◽  
Tegumentary Leishmaniasis ◽  
Response Profile ◽  
American Tegumentary Leishmaniasis ◽  
Cellular Immune

scholarly journals OX40+ T lymphocytes and IFN-γ are associated with American tegumentary leishmaniasis pathogenesis

2012 ◽  
Vol 87 (6) ◽  
pp. 851-855
Author(s):  
Patrícia Luciana Batista Domingos ◽  
Agostinho Gonçalves Viana ◽  
Carlos Alberto de Carvalho Fraga ◽  
Paulo Rogério Ferreti Bonan
Keyword(s):  
Immune Response ◽  
Cutaneous Leishmaniasis ◽  
Healing Process ◽  
Public Health Problem ◽  
Leishmania Species ◽  
Tissue Samples ◽  
Protein Levels ◽  
Skin Ulcers ◽  
American Tegumentary Leishmaniasis ◽  
Ifn Γ

BACKGROUND: Leishmaniases are zoonoses considered a public health problem, representing a complex group of diseases with a broad clinical spectrum and epidemiological diversity. Leishmaniasis is caused by several species of protozoa of the genus Leishmania. The evolution of the pathology and the resolution of the leishmaniasis are dependent mainly on the Leishmania species involved, although the cytokine profile plays an important role in the development of the immune response. OBJECTIVES: The purpose of our study was to evaluate the immune response of patients affected by lesions of cutaneous leishmaniasis by immunostaining of the OX40, CD20, IFN-γ and IL-4 proteins. METHODS: The tissue samples were collected from indolent skin ulcers confirmed as cutaneous leishmaniasis of 41 patients aged between six and 90 years. The lesions were submitted to OX40, CD20, INF-γ and IL-4 immunolabeling. RESULTS: We observed a statistically significant higher expression of IFN-γ compared with IL-4 (p=0.009). Besides, OX40 had higher expression when compared with CD20 (p<0.001). CONCLUSION: The present study indicates that the immune response in lesions of cutaneous leishmaniasis is associated with a healing process, which can be explained by the higher expression of IFN-γ when compared with IL4 protein levels.

scholarly journals Identification and purification of immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine (Leishvacin®)

2003 ◽  
Vol 36 (2) ◽  
pp. 193-199 ◽  
Author(s):  
Sandra Regina Afonso Cardoso ◽  
João Carlos França da Silva ◽  
Roberto Teodoro da Costa ◽  
Wilson Mayrink ◽  
Maria Norma Melo ◽  
...  
Keyword(s):  
Immune Response ◽  
Statistical Difference ◽  
Challenge Infection ◽  
Control Groups ◽  
Corynebacterium Parvum ◽  
Polyacrylamide Electrophoresis ◽  
Molecular Weights ◽  
Tegumentary Leishmaniasis ◽  
American Tegumentary Leishmaniasis ◽  
Immunogenic Proteins

Immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine against American tegumentary leishmaniasis (Leishvacin®), produced by Biobrás (Biochemistry of Brazil ), Montes Claros, State of Minas Gerais, Brazil, were identified and purified by polyacrylamide electrophoresis gel and electroelution. C57BL/10 mice were vaccinated with proteins with estimated molecular weights of 42, 46, 63, 66, 73, 87, 97, and 160kDa in three doses of 30µg of each protein at 15-day intervals combined with 250µg of Corynebacterium parvum followed by a challenge infection with 10(5) infective promastigotes from Leishmania (Leishmania) amazonensis. The ability of these proteins to induce immune response and protection was analyzed. No statistical difference was observed in the level of IFN-g induced by proteins in vaccinated groups in comparison with control groups. Six months after challenge infection, protection levels of 28.57; 42.86; 57.14; 42.86; 42.86, 57.14; 42.86 and 57.14% were demonstrated for each purified protein.

Immune response induced by New World Leishmania species in C57BL/6 mice

2004 ◽  
Vol 94 (3) ◽  
pp. 207-212 ◽  
Author(s):  
Tatiani Uceli Maioli ◽  
Erica Takane ◽  
Rosa Maria Esteves Arantes ◽  
Juliana Lopes Rangel Fietto ◽  
Lu�s Carlos Crocco Afonso
Keyword(s):  
Immune Response ◽  
New World ◽  
Leishmania Species

Evaluation of antigens from various Leishmania species in a Western blot for diagnosis of American tegumentary leishmaniasis.

2002 ◽  
Vol 66 (1) ◽  
pp. 91-102 ◽  
Author(s):  
Cely Cristina Martins Gonçalves ◽  
Benício Alves De Abreu Filho ◽  
Karoline Rocha Maia ◽  
Rafael Costacurta ◽  
José Vitor Jankevicius ◽  
...  
Keyword(s):  
Western Blot ◽  
Leishmania Species ◽  
Tegumentary Leishmaniasis ◽  
American Tegumentary Leishmaniasis

scholarly journals Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species

2016 ◽  
Vol 55 (2) ◽  
pp. 495-503 ◽  
Author(s):  
Camila Massae Sato ◽  
Maria Carmen Arroyo Sanchez ◽  
Beatriz Julieta Celeste ◽  
Malcolm S. Duthie ◽  
Jeffrey Guderian ◽  
...  
Keyword(s):  
Infectious Diseases ◽  
Leishmania Species ◽  
Delayed Type Hypersensitivity ◽  
Reactivity Index ◽  
Content Type ◽  
Tegumentary Leishmaniasis ◽  
American Tegumentary Leishmaniasis ◽  
Healthy Control ◽  
Low Sensitivity ◽  
Variable Performance

ABSTRACTAmerican tegumentary leishmaniasis (ATL) (also known as cutaneous leishmaniasis [CL]) is caused by various species of protozoa of the genusLeishmania. The diagnosis is achieved on a clinical, epidemiological, and pathological basis, supported by positive parasitological exams and demonstration of leishmanin delayed-type hypersensitivity. Serological assays are not routinely used in the diagnosis because many are considered to have low sensitivity and the particularLeishmaniaspecies causing the disease can lead to variable performance. In the present study, we generated recombinant versions of two highly conservedLeishmaniaproteins,Leishmania(Viannia)braziliensis-derived Lb8E and Lb6H, and evaluated both in enzyme-linked immunosorbent assays (ELISA). Recombinant Lb6H (rLb6H) had better performance and reacted with 100.0% of the ATL and 89.4% of the VL samples. These reactions with rLb6H were highly specific (98.5%) when compared against those for samples from healthy control individuals. We then assessed rLb6H against sera from ATL patients infected with different species ofLeishmaniaprevalent in Brazil [Leishmania(Leishmania)amazonensis,L. (Viannia)braziliensis, andL. (V.)guyanensis] and samples from patients with other infectious diseases. In analyses of 500 sera, ELISA using rLb6H detected all 219 ATL samples (sensitivity of 100.0%) with an overall specificity of 93.9% (considering healthy individuals and other infectious diseases patients). Only a minority of samples from Chagas disease patients possessed antibodies against rLb6H, and all of these responses were low (with a highest reactivity index of 2.2). Taken together, our data support further evaluation of rLb6H and the potential for its routine use in the serological diagnosis of ATL.

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