scholarly journals Synergism therapeutic and immunoregulatory effects of Albendazole + rAd-mIL-28B against Echinococcosis in experiment-infected mice with protoscoleces

2021 ◽  
Vol 15 (11) ◽  
pp. e0009927
Author(s):  
Yan Zhang ◽  
Jianghua Wang ◽  
Qingxia Yang ◽  
Zhi Li ◽  
Xiaoying Xu ◽  
...  
Keyword(s):  
Untreated Control ◽  
Therapeutic Agent ◽  
Germinal Layer ◽  
Severe Damage ◽  
New Agents ◽  
Tnf Α ◽  
The World ◽  
Wet Weight ◽  
Ifn Γ ◽  
Laminated Layer

The metacestode stage of Echinococcus granulosus can cause cystic echinococcosis (CE), which still widely occurs around the world. Since the early 1970s, benzimidazoles have been shown to inhibit the growth of cysts and used to treat CE. However, benzimidazoles are still ineffective in 20%-40% of cases. In order to explore the new agents against CE, we have investigated the therapeutic effect of the recombinant adenoviral vector expressing mouse IL-28B (rAd-mIL-28B) on protoscoleces-infected mice. In our study, we successfully established the model mice which infected with protoscoleces intraperitoneally. At 18 weeks post-infection, the mice received rAd-mIL-28B (1×107 PFU) weekly by intramuscular injection for 6 weeks. Compared with the untreated control (13.1 ± 2.2 g), there was a significant reduction in cysts wet weight in rAd-mIL-28B group (8.3 ± 3.5 g) (P < 0.05), especially in Albendazole (ABZ) + rAd-mIL-28B group (5.8 ± 1.4 g) (P < 0.01). We also observed the severe damage of the germinal layer and the laminated layer of cysts after treatment. rAd-mIL-28B group showed a prominent increase in the level of Th1 type cytokines (such as IFN-γ, IL-2 and TNF-α). Meanwhile, the frequency of Foxp3+ T cells was decreased in the rAd-mIL-28B group (4.83 ± 0.81%) and ABZ + rAd-mIL-28B group (4.60 ± 0.51%), comparing with the untreated group (8.13 ± 2.60%) (P < 0.05). In addition, compared with the untreated control (122.14 ± 81.09 pg/ml), the level of IFN-γ significantly increased in peritoneal fluid in the rAd-mIL-28B group (628.87 ± 467.16 pg/ml) (P < 0.05) and ABZ + rAd-mIL-28B group (999.76 ± 587.60 pg/ml) (P < 0.001). Taken together, it suggested that ABZ + IL-28B may be a potential therapeutic agent against CE.

scholarly journals Ultrastructure of Human Fertile Hydatid Cysts Using a Scanning Electron Microscope (SEM)

2020 ◽  
Vol 1 (3) ◽  
pp. 52-55
Author(s):  
Hadi M. Hamza Al-Mayali ◽  
Hind A. Abdul Kadhim
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Three Dimensional ◽  
Zoonotic Diseases ◽  
Hydatid Cysts ◽  
Germinal Layer ◽  
Transmission Electron ◽  
The World ◽  
Laminated Layer ◽  
Scanning Electron

Introduction: Echinococcosis and hydatidosis caused by the metacestode of Echinococcus granulosus are among the most important zoonotic diseases in the world. This study aims to study the ultrastructure of fertile hydatid cysts that infect humans using a scanning electron microscope (SEM). Materials and Methods: Twenty samples of human fertile hydatid cysts were collected from the human liver and lung after performing surgery operations and examined with an SEM. Results: The results of the electron microscopy with different magnifications revealed that the laminated layer (LL) consists of sheets that appeared more compact and aligned. The brood capsules appeared, consisting of a net of finger-shaped structures that emerged from bulges of various sizes and shapes. Conclusion: Under a transmission electron microscope, it was found that the LL had a coherent and flexible structure, settling on a three-dimensional microscopic network of hydrophilic fibers, with high humidity. These fibers were arranged irregularly and had a diameter of about 10 nm; therefore, the fibers adjacent to the germinal layer (GL) were possibly attached to microtriches of tegument, which reached a thickness of 1 mm in the LL.

scholarly journals Inhibitory Effects of Luteolin 7-Methyl Ether Isolated from Wikstroemia ganpi on Tnf-A/Ifn-Γ Mixture-Induced Inflammation in Human Keratinocyte

Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4387
Author(s):  
Jonghwan Jegal ◽  
Tae-Young Kim ◽  
No-June Park ◽  
Beom-Geun Jo ◽  
Geon-A. Jo ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Methyl Ether ◽  
Therapeutic Agent ◽  
Inflammatory Diseases ◽  
Hacat Cells ◽  
Inhibitory Effects ◽  
Gm Csf ◽  
Human Keratinocyte ◽  
Tnf Α ◽  
Ifn Γ

Plants of the genus Wikstroemia are traditionally used in China to treat various inflammatory diseases. The purpose of this study was to isolate the components of Wikstroemia ganpi (Siebold & Zucc.) Maxim., to evaluate their anti-atopic activities and to identify candidates with anti-atopic therapeutics. A total of 24 compounds were isolated by bioassay-guided separation, including one novel compound, which was tilianin 5-methyl ether. The anti-atopic activities of the isolated compounds were determined using TNF-α-treated RBL-2H3 cells and HaCaT cells. The mRNA expressions of IL-4, IL-6, GM-CSF, G-CSF and TRPV1 were reduced by luteolin 7-methyl ether. The study shows that the luteolin 7-methyl ether isolated from W. ganpi is a potential therapeutic agent for the treatment of atopic dermatitis.

Arsenic trioxide: a promising novel therapeutic agent for lymphoproliferative and autoimmune syndromes in MRL/lpr mice

Blood ◽  
2006 ◽  
Vol 108 (13) ◽  
pp. 3967-3975 ◽  
Author(s):  
Pierre Bobé ◽  
Danielle Bonardelle ◽  
Karim Benihoud ◽  
Paule Opolon ◽  
Mounira K. Chelbi-Alix
Keyword(s):  
Nitric Oxide ◽  
Arsenic Trioxide ◽  
Therapeutic Agent ◽  
Fas Ligand ◽  
Survival Rates ◽  
Tnf Α ◽  
Activated T Lymphocytes ◽  
Ifn Γ ◽  
Kidney Lesions

Abstract MRL/lpr mice develop a human lupuslike syndrome and, as in autoimmune lymphoproliferative syndrome (ALPS), massive lymphoproliferation due to inactivation of Fas-mediated apoptosis. Presently, no effective therapy exists for ALPS, and long term, therapies for lupus are hazardous. We show herein that arsenic trioxide (As2O3) is able to achieve quasi-total regression of antibody- and cell-mediated manifestations in MRL/lpr mice. As2O3 activated caspases and eliminated the activated T lymphocytes responsible for lymphoproliferation and skin, lung, and kidney lesions, leading to significantly prolonged survival rates. This treatment also markedly reduced anti-DNA autoantibody, rheumatoid factor, IL-18, IFN-γ, nitric oxide metabolite, TNF-α, Fas ligand, and IL-10 levels and immune-complex deposits in glomeruli. As2O3 restored cellular reduced glutathione levels, thereby limiting the toxic effect of nitric oxide, which is overproduced in MRL/lpr mice. Furthermore, As2O3 protected young animals against developing the syndrome and induced almost total disease disappearance in older affected mice, thereby demonstrating that it is a novel promising therapeutic agent for autoimmune diseases.

CD4+CD25- effector T cells from healthy controls and MS patients do not differ in mRNA expression of IFN-γ, IL-2, and TNF-α

2004 ◽  
Vol 31 (S 1) ◽  
Author(s):  
A Hug ◽  
J Haas ◽  
A Viehöver ◽  
B Fritz ◽  
B Storch-Hagenlocher ◽  
...  
Keyword(s):  
T Cells ◽  
Mrna Expression ◽  
Effector T Cells ◽  
Healthy Controls ◽  
Tnf Α ◽  
Ifn Γ

n. 174 (15): Dor On Line

Dor on line ◽  
2015 ◽  
Author(s):  
Paulo Barboni
Keyword(s):  
Tnf Α ◽  
On Line ◽  
Ifn Γ ◽  
Solieria Filiformis

Chamada da Edição   Caros leitores, iniciando o ano de 2015 o boletim Dor On Line traz a vocês o primeiro de uma série de editoriais tendo como tema as toxinas e o estudo da dor. Ainda, e complementando nossa edição anterior, trazemos resumos apresentados em congressos no ano de 2014, mostrando aqui alertas sobre alguns trabalhos discutidos em formato de pôster no 46º Congresso Brasileiro de Farmacologia e Terapêutica Experimental, promovido pela SBFTE, Sociedade Brasileira de Farmacologia e Terapêutica Experimental, em Fortaleza - CE, de 21 a 24 de outubro de 2014. Boa Leitura!   Alertas   1. O Bloqueio do receptor de potencial transitório A1 reduz a hiperalgesia em um modelo de neuralgia do trigêmeo. 2. Avaliação da eficácia terapêutica do extrato de óleo de peixe no tratamento da dor neuropática. 3. Ativação supraespinal da via das quinureninas contribui para a manutenção da dor neuropática. 4. Ativação da aldeído-desidrogenase 2 reduz a dor neuropática e adutos de 4-hidroxinoneal. 5. O envolvimento do sistema opioidérgico na analgesia induzida por enriquecimento ambiental. 6. NOD1 E NOD2 contribuem para a gênese da dor neuropática e estão envolvidos na ativação de células gliais. 7. Polissacarídeo sulfatado derivado de algas vermelha solieria filiformis reduz hipernocicepção mecânica na articulação temporomandibular de ratos durante a artrite induzida por zymosan. 8. IFN-γ induz indoleamina (2,3)-dioxigenase (IDO) na medula espinal que contribui para a gênese da dor neuropática. 9. TNF-α participa da hipernocicepção e da inflamação induzida por cristais de urato em camundongos. 10. Investigação do papel dos receptores Toll-like 9 (TLR9) no desenvolvimento e manutenção da dor crônica inflamatória e neuropática.

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