Identification of a Variable Number of Tandem Repeats Polymorphism and Characterization of LEF-1 Response Elements in the Promoter of the IDO1 Gene

Mycobacterium tuberculosis (MTB) stands out as the main causative agent of pulmonary tuberculosis (TB). However, nontuberculous mycobacteria (NTM) species also have the potential to infect and cause TB in susceptible individuals. The objective of this study was to identify NTM species that cause public health problems in remote areas. The study was carried out using 105 sputum smears obtained from patients from the Guna Yala Region of Panama with clinical signs suggestive of TB. DNA was extracted from sputum smears. Nontuberculous mycobacteria and MTB were characterized using polymerase chain reaction restriction analysis (hsp65, rpob) and an evaluation of 24-mycobacterial interspersed repetitive units–variable number of tandem repeats loci. Twenty-six Mycobacterium species were characterized; 19 (18%) were identified as MTB, and 7 (6.7%) were identified as NTM (four M. avium complex, two M. haemophilum, one M. tusciae). These results suggest that at least one in five cases of pulmonary TB among this population is caused by an NTM. Thus, identifying the bacteria causing pulmonary disease is key even in remote regions of the world where standard diagnosis and culture are not available. Strengthening the laboratory capacity within the Guna Yala Region is needed to identify NTM infections promptly.

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Identification and Characterization of Variable-Number Tandem Repeats in the Yersinia pestis Genome

Journal of Clinical Microbiology ◽

10.1128/jcm.39.9.3179-3185.2001 ◽

2001 ◽

Vol 39 (9) ◽

pp. 3179-3185 ◽

Cited By ~ 133

Author(s):

A. M. Klevytska ◽

L. B. Price ◽

J. M. Schupp ◽

P. L. Worsham ◽

J. Wong ◽

...

Keyword(s):

Yersinia Pestis ◽

Tandem Repeats ◽

Variable Number ◽

Variable Number Tandem Repeats ◽

Identification And Characterization

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Diagnosis and Molecular Characterization ofMycobacterium aviumsubsp.paratuberculosisfrom Dairy Cows in Colombia

Veterinary Medicine International ◽

10.4061/2011/352561 ◽

2011 ◽

Vol 2011 ◽

pp. 1-12 ◽

Cited By ~ 10

Author(s):

J. A. Fernández-Silva ◽

A. Abdulmawjood ◽

M. Bülte

Keyword(s):

Molecular Characterization ◽

Tandem Repeats ◽

Dairy Herd ◽

Variable Number ◽

Enzyme Linked Immunosorbent Assay ◽

Dairy Herds ◽

Serum Samples ◽

Tissue Samples ◽

Fecal Samples

The objective of this study was the serological, bacteriological and molecular diagnosis, as well as the molecular characterization ofMycobacterium aviumsubsp.paratuberculosis(Map) in adult cows of five Colombian dairy herds. Serum samples were tested by an indirect absorbed enzyme–linked immunosorbent assay (ELISA-C). All fecal samples were tested by pooled culture. After that, fecal samples of Map positive pools were tested individually by culture and polymerase chain reaction (PCR). In one herd, slurry and tissue samples from one animal were also taken and tested by PCR and culture. Map isolates were analyzed by the Multilocus Short Sequence Repeat (MLSSR) and the Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats (MIRU-VNTR) methods. ELISA produced positive results in 1.8% (6/329) of the animals and 40% (2/5) of the herds. Four fecal, two tissue, and two slurry samples from a herd were Map positive by culture and PCR. MLSSR and MIRU-VNTR revealed two different strain profiles among eight Map isolates recovered. This study reports the first molecular characterization of Map in one dairy herd in Colombia, the limitations for individual diagnosis of subclinical Map infections in cattle, and the usefulness of pooled fecal samples and environmental sampling for Map diagnosis.

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First Isolation of Leptospira Borgpetersenii Serovar Hardjobovis in Guanacos (Lama Guanicoe) in South America

10.21203/rs.3.rs-997246/v1 ◽

2021 ◽

Author(s):

Bibiana Brihuega ◽

Vanina Saraullo ◽

Mara Martinez ◽

Olivia Watanabe ◽

Micaela Hamer ◽

...

Keyword(s):

South America ◽

Tandem Repeats ◽

Variable Number ◽

Genetic Profile ◽

Lama Guanicoe ◽

Leptospira Borgpetersenii ◽

Wildlife Populations ◽

Variable Number Tandem Repeats ◽

Renal Infection

Abstract Background Leptospirosis is the most widespread zoonotic disease in the world. It is caused by pathogenic spirochetes of the genus Leptospira spp. and is maintained in nature through chronic renal infection of carrier animals, being rodents and other small mammals the main reservoirs. This bacterial genus is highly heterogeneous and divided into three clades (pathogenic, saprophyte and intermediate). Presence of pathogenic strains in wildlife populations is essential to monitor the epidemiological status of this disease worldwide. Methods In this study, we characterize an isolated strain of a Guanaco (Lama guanicoe) using Multiple Locus Variable number tandem repeats Analysis (MLVA) (Variable Number Tandem Repeats-VNTRs: 4, 7, 10, Lb4 and Lb5). To confirm the identity of the isolated strain, partial 16S rRNA sequencing was carried out. Phylogeny was constructed using Neighbor- joining. Results The pathogenic leptospiral strain isolated from Llama guanicoe had the genetic profile identical to L. borgpetersenii serovar Hardjobovis reference strain Sponselee. Conclusions To the best of our knowledge, this is the first isolation and genetic characterization of a pathogenic leptospiral strain in Guanacos in South America.

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Characterization of the variable-number tandem repeats in vrrA from different Bacillus anthracis isolates.

Applied and Environmental Microbiology ◽

10.1128/aem.63.4.1400-1405.1997 ◽

1997 ◽

Vol 63 (4) ◽

pp. 1400-1405 ◽

Cited By ~ 74

Author(s):

P J Jackson ◽

E A Walthers ◽

A S Kalif ◽

K L Richmond ◽

D M Adair ◽

...

Keyword(s):

Bacillus Anthracis ◽

Tandem Repeats ◽

Variable Number ◽

Variable Number Tandem Repeats

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Variable-Number Tandem Repeats as Molecular Markers for Biotypes of Pasteuria ramosa in Daphnia spp.

Applied and Environmental Microbiology ◽

10.1128/aem.02398-06 ◽

2007 ◽

Vol 73 (11) ◽

pp. 3715-3718 ◽

Cited By ~ 16

Author(s):

Laurence Mouton ◽

Guang Nong ◽

James F. Preston ◽

Dieter Ebert

Keyword(s):

Molecular Markers ◽

Tandem Repeats ◽

Genetic Characterization ◽

Variable Number ◽

Soil Samples ◽

Pasteuria Ramosa ◽

Variable Number Tandem Repeats

ABSTRACT Variable-number tandem repeats (VNTRs) have been identified in populations of Pasteuria ramosa, a castrating endobacterium of Daphnia species. The allelic polymorphisms at 14 loci in laboratory and geographically diverse soil samples showed that VNTRs may serve as biomarkers for the genetic characterization of P. ramosa isolates.

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Molecular Characterization of Phomopsis vexans Isolates of Eggplant of Bangladesh

Journal of Science Foundation ◽

10.3329/jsf.v8i1-2.14636 ◽

2013 ◽

Vol 8 (1-2) ◽

pp. 131-140 ◽

Cited By ~ 3

Author(s):

MM Islam ◽

M Asaduzzaman ◽

MB Meah

Keyword(s):

Genomic Dna ◽

Tandem Repeats ◽

Variable Number ◽

Banding Patterns ◽

Taq Polymerase ◽

Growing Seasons ◽

Polymerase Chain ◽

Phomopsis Vexans ◽

Template Dna

Forty-four isolates of Phomopsis vexans were collected from eggplant producing areas of Bangladesh during the 2008-2009 growing seasons. The polymerase chain reaction (PCR) -variable number of tandem repeats (VNTR) technique was used to develop molecular markers. The MR-20 primer amplified template DNA for all isolates studied and resulted in distinct bands. The banding patterns of P. vexans isolates were separated into five groups after results with Taq polymerase. The highest genomic DNA concentration 50 ng/?l was found in the isolates of 8, 14, 26, 27 and 38, and the lowest concentration of genomic DNA was found 12.5 ng/?l in the isolates of 1, 2, 5, 15, 17, 22, 30, 31, 33, 34, 36, 40, 41, 42 and 43. Based on the fingerprinting the 44 isolates of Phomopsis vexans were categorized into five different groups. DOI: http://dx.doi.org/10.3329/jsf.v8i1-2.14636 J. Sci. Foundation, 8(1&2): 131-140, June-December 2010

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