scholarly journals Effect of Topical Anaesthetics on Interstitial Colloid Osmotic Pressure in Human Subcutaneous Tissue Sampled by Wick Technique

PLoS ONE ◽  
2012 ◽  
Vol 7 (2) ◽  
pp. e31332 ◽  
Author(s):  
Hans Jørgen Timm Guthe ◽  
Torbjørn Nedrebø ◽  
Olav Tenstad ◽  
Helge Wiig ◽  
Ansgar Berg
Keyword(s):  
Osmotic Pressure ◽  
Subcutaneous Tissue ◽  
Colloid Osmotic Pressure ◽  
Wick Technique ◽  
Topical Anaesthetics

Transcapillary Starling forces using membrane osmometry

1980 ◽  
Vol 238 (6) ◽  
pp. H886-H888
Author(s):  
J. L. Christian ◽  
R. A. Brace
Keyword(s):  
Osmotic Pressure ◽  
Interstitial Fluid ◽  
Subcutaneous Tissue ◽  
Fluid Pressure ◽  
Colloid Osmotic Pressure ◽  
Interstitial Fluid Pressure ◽  
Tissue Samples ◽  
Small Pore ◽  
The Difference ◽  
Good Agreement

Membrane osmometry was used to estimate the four transcapillary Starling pressures in subcutaneous tissue of rats, guinea pigs, and dogs. Isolated subcutaneous tissue samples were either placed on a large-pore or small-pore osmometer that measured the interstitial fluid pressure (Pif) and the difference between the interstitial fluid pressure and the interstitial protein osmotic pressure (Pif-pi if), respectively. The colloid osmotic pressure of the interstitial fluid (pi if) was obtained from the difference in these two pressures. A plasma sample placed on the small-pore osmometer yielded the colloid osmotic pressure of the plasma proteins (pi c). Finally the capillary pressure (Pc) was calculated from the three other Starling forces. In the rat, guinea pig, and dog, respectively, the estimated Starling forces were as follows: Pif -2.2, -2.1, and -4.8 mmHg; pi if, 7.3, 4.8, and 4.4 mmHg; pi c, 21.3, 19.5, and 19.2 mmHg; and Pc, 11.8, 12.6, and 10.0 mmHg. A comparison with data obtained in other studies using different methods shows good agreement and strongly supports membrane osmometry as a method for measuring the Starling pressures in subcutaneous tissue.

Edema in Spawning Salmon

1975 ◽  
Vol 32 (12) ◽  
pp. 2538-2541 ◽  
Author(s):  
Alan R. Hargens ◽  
M. Perez
Keyword(s):  
Osmotic Pressure ◽  
Fresh Water ◽  
Fluid Pressure ◽  
Colloid Osmotic Pressure ◽  
Spawning Migration ◽  
Degenerative Changes ◽  
Wick Technique ◽  
Total Body

Salmon develop edema during their spawning migration from the sea to fresh water. As measured by the wick technique, fluid pressure in both subcutaneous and peritoneal compartments rises from negative values at sea to positive values in rivers. Concomitantly, blood colloid osmotic pressure falls during the spawning migration. Some of the degenerative changes leading to the death of postspawning salmon probably result from the total-body edema herein described.

Isolation of pulmonary interstitial fluid in rabbits by a modified wick technique

2001 ◽  
Vol 280 (5) ◽  
pp. L1057-L1065 ◽  
Author(s):  
Daniela Negrini ◽  
Alberto Passi ◽  
Katia Bertin ◽  
Federica Bosi ◽  
Helge Wiig
Keyword(s):  
Osmotic Pressure ◽  
Plasma Proteins ◽  
Regional Differences ◽  
Protein Concentration ◽  
Interstitial Fluid ◽  
Lung Parenchyma ◽  
Colloid Osmotic Pressure ◽  
Rabbit Lung ◽  
Pressure Plasma ◽  
Wick Technique

Interstitial fluid protein concentration (Cprotein) values in perivascular and peribronchial lung tissues were never simultaneously measured in mammals; in this study, perivascular and peribronchial interstitial fluids were collected from rabbits under control conditions and rabbits with hydraulic edema or lesional edema. Postmortem dry wicks were implanted in the perivascular and peribronchial tissues; after 20 min, the wicks were withdrawn and the interstitial fluid was collected to measure Cprotein and colloid osmotic pressure. Plasma, perivascular, and peribronchial Cproteinvalues averaged 6.4 ± 0.7 (SD), 3.7 ± 0.5, and 2.4 ± 0.7 g/dl, respectively, in control rabbits; 4.8 ± 0.7, 2.5 ± 0.6, and 2.4 ± 0.4 g/dl, respectively, in rabbits with hydraulic edema; and 5.1 ± 0.3, 4.3 ± 0.4 and 3.3 ± 0.6 g/dl, respectively, in rabbits with lesional edema. Contamination of plasma proteins from microvascular lesions during wick insertion was 14% of plasma Cprotein. In control animals, pulmonary interstitial Cprotein was lower than previous estimates from pre- and postnodal pulmonary lymph; furthermore, although the interstitium constitutes a continuum within the lung parenchyma, regional differences in tissue content seem to exist in the rabbit lung.

Colloid osmotic pressure changes of dog's blood exposed to different mixtures of CO2 and air

1978 ◽  
Vol 44 (2) ◽  
pp. 254-257 ◽  
Author(s):  
Y. Kakiuchi ◽  
A. B. DuBois ◽  
D. Gorenberg
Keyword(s):  
Red Blood Cells ◽  
Osmotic Pressure ◽  
Cell Volume ◽  
Blood Cells ◽  
Freezing Point ◽  
Colloid Osmotic Pressure ◽  
Red Cell ◽  
Freezing Point Depression ◽  
Pressure Changes ◽  
Different Levels

Hansen's membrane manometer method for measuring plasma colloid osmotic pressure was used to obtain the osmolality changes of dogs breathing different levels of CO2. Osmotic pressure was converted to osmolality by calibration of the manometer with saline and plasma, using freezing point depression osmometry. The addition of 10 vol% of CO2 to tonometered blood caused about a 2.0 mosmol/kg H2O increase of osmolality, or 1.2% increase of red blood cell volume. The swelling of the red blood cells was probably due to osmosis caused by Cl- exchanged for the HCO3- which was produced rapidly by carbonic anhydrase present in the red blood cells. The change in colloid osmotic pressure accompanying a change in co2 tension was measured on blood obtained from dogs breathing different CO2 mixtures. It was approximately 0.14 mosmol/kg H2O per Torr Pco2. The corresponding change in red cell volume could not be calculated from this because water can exchange between the plasma and tissues.

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