scholarly journals Correction: Human Osteoarthritic Cartilage Shows Reduced In Vivo Expression of IL-4, a Chondroprotective Cytokine that Differentially Modulates IL-1β-Stimulated Production of Chemokines and Matrix-Degrading Enzymes In Vitro

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e105819
Author(s):  
Keyword(s):  
Osteoarthritic Cartilage ◽  
Degrading Enzymes ◽  
In Vivo Expression ◽  
Matrix Degrading Enzymes

scholarly journals Effect ofAngelica sinensisPolysaccharides on OsteoarthritisIn VivoandIn Vitro: A Possible Mechanism to Promote Proteoglycans Synthesis

2013 ◽  
Vol 2013 ◽  
pp. 1-15 ◽  
Author(s):  
Jun Qin ◽  
Yan-song Liu ◽  
Jun Liu ◽  
Jing Li ◽  
Yang Tan ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Articular Chondrocytes ◽  
Interleukin 1 ◽  
Knee Joints ◽  
Protective Effects ◽  
Interleukin 1 Beta ◽  
Degrading Enzymes ◽  
Matrix Degrading Enzymes

This study investigated the effect ofAngelica sinensispolysaccharides (APS-3c) on rat osteoarthritis (OA) modelin vivoand rat interleukin-1-beta- (IL-1β-) stimulated chondrocytesin vitro. APS-3c was administrated into rat OA knee joints and had protective effects on rat OA cartilagein vivo. Primary rat articular chondrocytes were cotreated with APS-3c and IL-1β  in vitro. 2~50 μg/mL APS-3c had no effect on chondrocytes viability, whereas it increased the proteoglycans (PGs) synthesis inhibited by IL-1β. Microarray analysis showed that the significant changes were concentrated in the genes which were involved in PGs synthesis. RT-PCR confirmed that treatment with APS-3c increased the mRNA expression of aggrecan and glycosyltransferases (GTs) inhibited by IL-1βbut did not affect the mRNA expression of matrix-degrading enzymes. These results indicate that APS-3c can improve PGs synthesis of chondrocytes on rat OA modelin vivoand IL-1β-stimulated chondrocytesin vitro, which is due to the promotion of the expression of aggrecan and GTs involved in PGs synthesis but not the inhibition of the expression of matrix-degrading enzymes. Our findings suggest the clinical relevance of APS-3c in the prospective of future alternative medical treatment for OA.

In vitro and in vivo expression of a nephritogenic Ig heavy chain determinant: Pathogenic autoreactivity requires permissive light chains

2001 ◽  
Vol 79 (3) ◽  
pp. 222-230 ◽  
Author(s):  
Brenda G Cooperstone ◽  
Mohammed M Rahman ◽  
Earl H Rudolph ◽  
Mary H Foster
Keyword(s):  
Heavy Chain ◽  
Light Chains ◽  
In Vivo Expression ◽  
Ig Heavy Chain

In Vitro and in Vivo Expression α7 Integrin and Desmin Define the Primary and Secondary Myogenic Lineages

1993 ◽  
Vol 156 (1) ◽  
pp. 209-229 ◽  
Author(s):  
Mindy George-Weinstein ◽  
Rachel F. Foster ◽  
Jacquelyn V. Gerhart ◽  
Stephen J. Kaufman
Keyword(s):  
In Vivo Expression

scholarly journals The TAT Protein Transduction Domain as an Intra-Articular Drug Delivery Technology

Cartilage ◽  
2020 ◽  
pp. 194760352095939
Author(s):  
Sarah E. Mailhiot ◽  
Matthew A. Thompson ◽  
Akiko E. Eguchi ◽  
Sabrina E. Dinkel ◽  
Martin K. Lotz ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Genetic Recombination ◽  
Great Promise ◽  
Tat Protein ◽  
Binding Studies ◽  
Osteoarthritic Cartilage ◽  
Peptide Transduction ◽  
Delivery Technology

Objective Intra-articular drug delivery holds great promise for the treatment of joint diseases such as osteoarthritis. The objective of this study was to evaluate the TAT peptide transduction domain (TAT-PTD) as a potential intra-articular drug delivery technology for synovial joints. Design Experiments examined the ability of TAT conjugates to associate with primary chondrocytes and alter cellular function both in vitro and in vivo. Further experiments examined the ability of the TAT-PTD to bind to human osteoarthritic cartilage. Results The results show that the TAT-PTD associates with chondrocytes, is capable of delivering siRNA for chondrocyte gene knockdown, and that the recombinant enzyme TAT-Cre is capable of inducing in vivo genetic recombination within the knee joint in a reporter mouse model. Last, binding studies show that osteoarthritic cartilage preferentially uptakes the TAT-PTD from solution. Conclusions The results suggest that the TAT-PTD is a promising delivery strategy for intra-articular therapeutics.

The measles virus matrix gene and gene product defined by in vitro and in vivo expression

Virology ◽  
1987 ◽  
Vol 157 (2) ◽  
pp. 497-508 ◽  
Author(s):  
Timothy C. Wong ◽  
Gregory Wipf ◽  
Akiko Hirano
Keyword(s):  
Gene Product ◽  
Measles Virus ◽  
Matrix Gene ◽  
In Vivo Expression
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