scholarly journals C-STrap Sample Preparation Method—In-Situ Cysteinyl Peptide Capture for Bottom-Up Proteomics Analysis in the STrap Format

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0138775 ◽  
Author(s):  
Alexandre Zougman ◽  
Rosamonde E. Banks
PROTEOMICS ◽  
2014 ◽  
Vol 14 (9) ◽  
pp. 1006-1000 ◽  
Author(s):  
Alexandre Zougman ◽  
Peter J. Selby ◽  
Rosamonde E. Banks

Author(s):  
Jian-Shing Luo ◽  
Hsiu Ting Lee

Abstract Several methods are used to invert samples 180 deg in a dual beam focused ion beam (FIB) system for backside milling by a specific in-situ lift out system or stages. However, most of those methods occupied too much time on FIB systems or requires a specific in-situ lift out system. This paper provides a novel transmission electron microscopy (TEM) sample preparation method to eliminate the curtain effect completely by a combination of backside milling and sample dicing with low cost and less FIB time. The procedures of the TEM pre-thinned sample preparation method using a combination of sample dicing and backside milling are described step by step. From the analysis results, the method has applied successfully to eliminate the curtain effect of dual beam FIB TEM samples for both random and site specific addresses.


Micron ◽  
2014 ◽  
Vol 58 ◽  
pp. 25-31 ◽  
Author(s):  
Neda Dalili ◽  
Peng Li ◽  
Martin Kupsta ◽  
Qi Liu ◽  
Douglas G. Ivey

Nano Select ◽  
2020 ◽  
Vol 1 (4) ◽  
pp. 413-418
Author(s):  
Carmel Mary Esther Alphonse ◽  
Mohan Muralikrishna Garlapati ◽  
Sven Hilke ◽  
Gerhard Wilde

Author(s):  
Hagit Barda ◽  
Irina Geppert ◽  
Avraham Raz ◽  
Rémy Berthier

Abstract An experimental setup is presented, that allows in-situ Transition Electron Microscopy (TEM) investigation of void formation and growth within fully embedded interconnect structure, as a response to an external bias. A special TEM holder is employed to perform in-situ I-V measurements across the Via, simultaneously monitoring the morphological and chemical changes surrounding the void. This work presents in detail a Focused Ion Beam (FIB) based sample preparation method that allows the analysis of a Cu single Via structure found in the advanced microelectronic 14nm FinFET technology, as well as preliminary TEM observations.


2014 ◽  
Vol 20 (S3) ◽  
pp. 1514-1515 ◽  
Author(s):  
Xiangli Zhong ◽  
M. G. Burke ◽  
S. Schilling ◽  
S.J. Haigh ◽  
M. A. ◽  
...  

Author(s):  
Swaminathan Subramanian ◽  
Khiem Ly ◽  
Tony Chrastecky

Abstract Visualization of dopant related anomalies in integrated circuits is extremely challenging. Cleaving of the die may not be possible in practical failure analysis situations that require extensive electrical fault isolation, where the failing die can be submitted of scanning probe microscopy analysis in various states such as partially depackaged die, backside thinned die, and so on. In advanced technologies, the circuit orientation in the wafer may not align with preferred crystallographic direction for cleaving the silicon or other substrates. In order to overcome these issues, a focused ion beam lift-out based approach for site-specific cross-section sample preparation is developed in this work. A directional mechanical polishing procedure to produce smooth damage-free surface for junction profiling is also implemented. Two failure analysis applications of the sample preparation method to visualize junction anomalies using scanning microwave microscopy are also discussed.


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