scholarly journals RNA-seq reveals differentially expressed genes in rice (Oryza sativa) roots during interactions with plant-growth promoting bacteria, Azospirillum brasilense

PLoS ONE ◽  
2019 ◽  
Vol 14 (5) ◽  
pp. e0217309 ◽  
Author(s):  
Jacklyn Thomas ◽  
Ha Ram Kim ◽  
Yasir Rahmatallah ◽  
Grant Wiggins ◽  
Qinqing Yang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Plant Growth ◽  
Differentially Expressed Genes ◽  
Azospirillum Brasilense ◽  
Differentially Expressed ◽  
Plant Growth Promoting Bacteria ◽  
Rna Seq ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Compatibility of Azospirillum brasilense with Pesticides Used for Treatment of Maize Seeds

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Mariana S. Santos ◽  
Artur B. L. Rondina ◽  
Marco A. Nogueira ◽  
Mariangela Hungria
Keyword(s):  
Plant Growth ◽  
Root Hair ◽  
Azospirillum Brasilense ◽  
Plant Protection ◽  
Plant Growth Promoting Bacteria ◽  
Root Volume ◽  
Maize Seeds ◽  
Plant Growth Promoting ◽  
Growth Promoting

Seed treatment with chemical pesticides is commonly used as an initial plant protection procedure against pests and diseases. However, the use of such chemicals may impair the survival and performance of beneficial microorganisms introduced via inoculants, such as the plant growth-promoting bacterium Azospirillum brasilense. We assessed the compatibility between the most common pesticide used in Brazil for the treatment of maize seeds, composed of two fungicides, and one insecticide, with the commercial strains Ab-V5 and Ab-V6 of A. brasilense, and evaluated the impacts on initial plant development. The toxicity of the pesticide to A. brasilense was confirmed, with an increase in cell mortality after only 24 hours of exposure in vitro. Seed germination and seedling growth were not affected neither by the A. brasilense nor by the pesticide. However, under greenhouse conditions, the pesticide affected root volume and dry weight and root-hair incidence, but the toxicity was alleviated by the inoculation with A. brasilense for the root volume and root-hair incidence parameters. In maize seeds inoculated with A. brasilense, the pesticide negatively affected the number of branches, root-hair incidence, and root-hair length. Therefore, new inoculant formulations with cell protectors and the development of compatible pesticides should be searched to guarantee the benefits of inoculation with plant growth-promoting bacteria.

scholarly journals Quantification of Azospirillum brasilense FP2 Bacteria in Wheat Roots by Strain-Specific Quantitative PCR

2015 ◽  
Vol 81 (19) ◽  
pp. 6700-6709 ◽  
Author(s):  
Maria Isabel Stets ◽  
Sylvia Maria Campbell Alqueres ◽  
Emanuel Maltempi Souza ◽  
Fábio de Oliveira Pedrosa ◽  
Michael Schmid ◽  
...  
Keyword(s):  
Plant Growth ◽  
Quantitative Pcr ◽  
Dna Sequences ◽  
Azospirillum Brasilense ◽  
Plant Growth Promoting Bacteria ◽  
Genome Sequences ◽  
Wheat Roots ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACTAzospirillumis a rhizobacterial genus containing plant growth-promoting species associated with different crops worldwide.Azospirillum brasilensestrains exhibit a growth-promoting effect by means of phytohormone production and possibly by N2fixation. However, one of the most important factors for achieving an increase in crop yield by plant growth-promoting rhizobacteria is the survival of the inoculant in the rhizosphere, which is not always achieved. The objective of this study was to develop quantitative PCR protocols for the strain-specific quantification ofA. brasilenseFP2. A novel approach was applied to identify strain-specific DNA sequences based on a comparison of the genomic sequences within the same species. The draft genome sequences ofA. brasilenseFP2 and Sp245 were aligned, and FP2-specific regions were filtered and checked for other possible matches in public databases. Strain-specific regions were then selected to design and evaluate strain-specific primer pairs. The primer pairs AzoR2.1, AzoR2.2, AzoR5.1, AzoR5.2, and AzoR5.3 were specific for theA. brasilenseFP2 strain. These primer pairs were used to monitor quantitatively the population ofA. brasilensein wheat roots under sterile and nonsterile growth conditions. In addition, coinoculations with other plant growth-promoting bacteria in wheat were performed under nonsterile conditions. The results showed thatA. brasilenseFP2 inoculated into wheat roots is highly competitive and achieves high cell numbers (∼107CFU/g [fresh weight] of root) in the rhizosphere even under nonsterile conditions and when coinoculated with other rhizobacteria, maintaining the population at rather stable levels for at least up to 13 days after inoculation. The strategy used here can be applied to other organisms whose genome sequences are available.

Improved establishment and development of three cactus species inoculated withAzospirillum brasilensetransplanted into disturbed urban desert soil

1999 ◽  
Vol 45 (6) ◽  
pp. 441-451 ◽  
Author(s):  
Yoav Bashan ◽  
Adriana Rojas ◽  
M Esther Puente
Keyword(s):  
Soil Erosion ◽  
Plant Growth ◽  
Azospirillum Brasilense ◽  
Soil Reclamation ◽  
High Survival ◽  
Plant Growth Promoting Bacteria ◽  
Disturbed Areas ◽  
Small Roots ◽  
Plant Growth Promoting ◽  
Growth Promoting

Survival and development of cactus transplants in urban, disturbed areas of the desert near La Paz, Baja California Sur, Mexico, was monitored. Young plants of three species of pachycereid cacti (Pachycereus pringlei, Stenocereus thurberi, and Lophocereus schottii) inoculated with the plant growth promoting bacterium Azospirillum brasilense in an eroded area (a dirt road) had a high survival rate and developed more rapidly compared with uninoculated control plants during a 3.5-year period after transplantation. Soil erosion in the inoculated experimental area diminished. Small, but significant soil accumulated in association with the growth of cactus roots into the wind-deposited dust. One demonstrated mechanism for stabilizing dust was by the upward growth of small roots during the rainy season into the deposited dust. Azospirillum brasilense survived well in the rhizospheres of these cacti for 2 years, but not in root-free soil. This study demonstrated the feasibility of using bacterial inoculation of cacti to enhance their establishment in disturbed areas, with the potential to stabilize soil.Key words: Azospirillum, beneficial bacteria, cactus, plant inoculation, plant growth promoting bacteria, PGPR, soil erosion, soil reclamation.

The effect of native and ACC deaminase-containing Azospirillum brasilense Cd1843 on the rooting of carnation cuttings

2005 ◽  
Vol 51 (6) ◽  
pp. 511-514 ◽  
Author(s):  
Qiaosi Li ◽  
Saleema Saleh-Lakha ◽  
Bernard R Glick
Keyword(s):  
Plant Growth ◽  
Azospirillum Brasilense ◽  
Indoleacetic Acid ◽  
Acc Deaminase ◽  
Plant Growth Promoting Bacteria ◽  
Indolebutyric Acid ◽  
Proposed Model ◽  
Plant Growth Promoting ◽  
Growth Promoting

Carnation cuttings treated with non-transformed and 1-aminocyclopropane (ACC) deaminase-containing Azospirillum brasilense Cd1843 produced significantly more roots than untreated controls and fewer roots than cuttings treated with 0.1% indolebutyric acid (IBA). The roots produced by cuttings treated with ACC deaminase-containing Azospirillum brasilense Cd1843 were the longest roots resulting from any of the treatments, followed by non-transformed Azospirillum brasilense Cd1843, 0.1% IBA, and treatment with water. The results are interpreted in terms of a previously proposed model of bacterial promotion of plant growth by ACC deaminase and indoleacetic acid, and may have implications for the use of plant growth-promoting bacteria in the flower industry.Key words: ACC deaminase, carnation, cuttings, rooting, Azospirillum brasilense.

Real time PCR detection targeting nifA gene of plant growth promoting bacteria Azospirillum brasilense strain FP2 in maize roots

Symbiosis ◽  
2013 ◽  
Vol 61 (3) ◽  
pp. 125-133 ◽  
Author(s):  
Alexandro Cézar Faleiro ◽  
Tomás Pellizzaro Pereira ◽  
Eliandro Espindula ◽  
Fábio Cristiano Angonesi Brod ◽  
Ana Carolina Maisonnave Arisi
Keyword(s):  
Plant Growth ◽  
Real Time ◽  
Real Time Pcr ◽  
Azospirillum Brasilense ◽  
Pcr Detection ◽  
Plant Growth Promoting Bacteria ◽  
Maize Roots ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Nifa Gene
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