scholarly journals Compatibility of Azospirillum brasilense with Pesticides Used for Treatment of Maize Seeds

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Mariana S. Santos ◽  
Artur B. L. Rondina ◽  
Marco A. Nogueira ◽  
Mariangela Hungria
Keyword(s):  
Plant Growth ◽  
Root Hair ◽  
Azospirillum Brasilense ◽  
Plant Protection ◽  
Plant Growth Promoting Bacteria ◽  
Root Volume ◽  
Maize Seeds ◽  
Plant Growth Promoting ◽  
Growth Promoting

Seed treatment with chemical pesticides is commonly used as an initial plant protection procedure against pests and diseases. However, the use of such chemicals may impair the survival and performance of beneficial microorganisms introduced via inoculants, such as the plant growth-promoting bacterium Azospirillum brasilense. We assessed the compatibility between the most common pesticide used in Brazil for the treatment of maize seeds, composed of two fungicides, and one insecticide, with the commercial strains Ab-V5 and Ab-V6 of A. brasilense, and evaluated the impacts on initial plant development. The toxicity of the pesticide to A. brasilense was confirmed, with an increase in cell mortality after only 24 hours of exposure in vitro. Seed germination and seedling growth were not affected neither by the A. brasilense nor by the pesticide. However, under greenhouse conditions, the pesticide affected root volume and dry weight and root-hair incidence, but the toxicity was alleviated by the inoculation with A. brasilense for the root volume and root-hair incidence parameters. In maize seeds inoculated with A. brasilense, the pesticide negatively affected the number of branches, root-hair incidence, and root-hair length. Therefore, new inoculant formulations with cell protectors and the development of compatible pesticides should be searched to guarantee the benefits of inoculation with plant growth-promoting bacteria.

scholarly journals Quantification of Azospirillum brasilense FP2 Bacteria in Wheat Roots by Strain-Specific Quantitative PCR

2015 ◽  
Vol 81 (19) ◽  
pp. 6700-6709 ◽  
Author(s):  
Maria Isabel Stets ◽  
Sylvia Maria Campbell Alqueres ◽  
Emanuel Maltempi Souza ◽  
Fábio de Oliveira Pedrosa ◽  
Michael Schmid ◽  
...  
Keyword(s):  
Plant Growth ◽  
Quantitative Pcr ◽  
Dna Sequences ◽  
Azospirillum Brasilense ◽  
Plant Growth Promoting Bacteria ◽  
Genome Sequences ◽  
Wheat Roots ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACTAzospirillumis a rhizobacterial genus containing plant growth-promoting species associated with different crops worldwide.Azospirillum brasilensestrains exhibit a growth-promoting effect by means of phytohormone production and possibly by N2fixation. However, one of the most important factors for achieving an increase in crop yield by plant growth-promoting rhizobacteria is the survival of the inoculant in the rhizosphere, which is not always achieved. The objective of this study was to develop quantitative PCR protocols for the strain-specific quantification ofA. brasilenseFP2. A novel approach was applied to identify strain-specific DNA sequences based on a comparison of the genomic sequences within the same species. The draft genome sequences ofA. brasilenseFP2 and Sp245 were aligned, and FP2-specific regions were filtered and checked for other possible matches in public databases. Strain-specific regions were then selected to design and evaluate strain-specific primer pairs. The primer pairs AzoR2.1, AzoR2.2, AzoR5.1, AzoR5.2, and AzoR5.3 were specific for theA. brasilenseFP2 strain. These primer pairs were used to monitor quantitatively the population ofA. brasilensein wheat roots under sterile and nonsterile growth conditions. In addition, coinoculations with other plant growth-promoting bacteria in wheat were performed under nonsterile conditions. The results showed thatA. brasilenseFP2 inoculated into wheat roots is highly competitive and achieves high cell numbers (∼107CFU/g [fresh weight] of root) in the rhizosphere even under nonsterile conditions and when coinoculated with other rhizobacteria, maintaining the population at rather stable levels for at least up to 13 days after inoculation. The strategy used here can be applied to other organisms whose genome sequences are available.

scholarly journals Acclimatization of Pouteria gardeneriana Radlk micropropagated plantlets: role of in vitro rooting and plant growth–promoting bacteria

2021 ◽  
pp. 100209
Author(s):  
Mariluza Silva Leite ◽  
Tainara Eler Furtado Pinto ◽  
Agda Rabelo Centofante ◽  
Aurélio Rubio Neto ◽  
Fabiano Guimarães Silva ◽  
...  
Keyword(s):  
Plant Growth ◽  
In Vitro Rooting ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Plant Growth-Promoting Bacteria as an Emerging Tool to Manage Bacterial Rice Pathogens

2021 ◽  
Vol 9 (4) ◽  
pp. 682
Author(s):  
Mohamad Syazwan Ngalimat ◽  
Erneeza Mohd Hata ◽  
Dzarifah Zulperi ◽  
Siti Izera Ismail ◽  
Mohd Razi Ismail ◽  
...  
Keyword(s):  
Plant Growth ◽  
Large Scale ◽  
Plant Growth Promoting Bacteria ◽  
Promising Alternative ◽  
Rice Plants ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Rice Pathogens ◽  
Grain Losses

As a major food crop, rice (Oryza sativa) is produced and consumed by nearly 90% of the population in Asia with less than 9% produced outside Asia. Hence, reports on large scale grain losses were alarming and resulted in a heightened awareness on the importance of rice plants’ health and increased interest against phytopathogens in rice. To serve this interest, this review will provide a summary on bacterial rice pathogens, which can potentially be controlled by plant growth-promoting bacteria (PGPB). Additionally, this review highlights PGPB-mediated functional traits, including biocontrol of bacterial rice pathogens and enhancement of rice plant’s growth. Currently, a plethora of recent studies address the use of PGPB to combat bacterial rice pathogens in an attempt to replace existing methods of chemical fertilizers and pesticides that often lead to environmental pollutions. As a tool to combat bacterial rice pathogens, PGPB presented itself as a promising alternative in improving rice plants’ health and simultaneously controlling bacterial rice pathogens in vitro and in the field/greenhouse studies. PGPB, such as Bacillus, Pseudomonas, Enterobacter, Streptomyces, are now very well-known. Applications of PGPB as bioformulations are found to be effective in improving rice productivity and provide an eco-friendly alternative to agroecosystems.

scholarly journals De novo genome assembly of Bacillus altitudinis 19RS3 and Bacillus altitudinis T5S-T4, two plant growth-promoting bacteria isolated from Ilex paraguariensis St. Hil. (yerba mate)

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0248274
Author(s):  
Iliana Julieta Cortese ◽  
María Lorena Castrillo ◽  
Andrea Liliana Onetto ◽  
Gustavo Ángel Bich ◽  
Pedro Darío Zapata ◽  
...  
Keyword(s):  
Plant Growth ◽  
De Novo ◽  
Draft Genome ◽  
Ilex Paraguariensis ◽  
Plant Growth Promoting Bacteria ◽  
De Novo Genome Assembly ◽  
Bacillus Altitudinis ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth-promoting bacteria (PGPB) are a heterogeneous group of bacteria that can exert beneficial effects on plant growth directly or indirectly by different mechanisms. PGPB-based inoculant formulation has been used to replace chemical fertilizers and pesticides. In our previous studies, two endophytic endospore-forming bacteria identified as Bacillus altitudinis were isolated from roots of Ilex paraguariensis St. Hil. seedlings and selected for their plant growth-promoting (PGP) properties shown in vitro and in vivo. The purposes of this work were to assemble the genomes of B. altitudinis 19RS3 and T5S-T4, using different assemblers available for Windows and Linux and to select the best assembly for each strain. Both genomes were also automatically annotated to detect PGP genes and compare sequences with other genomes reported. Library construction and draft genome sequencing were performed by Macrogen services. Raw reads were filtered using the Trimmomatic tool. Genomes were assembled using SPAdes, ABySS, Velvet, and SOAPdenovo2 assemblers for Linux, and Geneious and CLC Genomics Workbench assemblers for Windows. Assembly evaluation was done by the QUAST tool. The parameters evaluated were the number of contigs ≥ 500 bp and ≥ 1000 bp, the length of the longest contig, and the N50 value. For genome annotation PROKKA, RAST, and KAAS tools were used. The best assembly for both genomes was obtained using Velvet. The B. altitudinis 19RS3 genome was assembled into 15 contigs with an N50 value of 1,943,801 bp. The B. altitudinis T5S-T4 genome was assembled into 24 contigs with an N50 of 344,151 bp. Both genomes comprise several genes related to PGP mechanisms, such as those for nitrogen fixation, iron metabolism, phosphate metabolism, and auxin biosynthesis. The results obtained offer the basis for a better understanding of B. altitudinis 19RS3 and T5S-T4 and make them promissory for bioinoculant development.

scholarly journals Initial performance of maize in response to humic acids and plant growth-promoting bacteria

Revista CERES ◽  
2018 ◽  
Vol 65 (3) ◽  
pp. 271-277 ◽  
Author(s):  
Raphael Oliveira de Melo ◽  
Hend Pereira de Oliveira ◽  
Klever Cristiano Silveira ◽  
Lílian Estrela Borges Baldotto ◽  
Marihus Altoé Baldotto
Keyword(s):  
Plant Growth ◽  
Humic Acids ◽  
Most Probable Number ◽  
Plant Growth Promoting Bacteria ◽  
Probable Number ◽  
Combined Application ◽  
Maize Seeds ◽  
Burkholderia Gladioli ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Seed treatment with inoculants based on plant growth-promoting bacteria (PGPB) or the application of humic acids (HA) may increase the productivity of plants of agricultural interest. The hypothesis of this work is that it is possible to combine the effect of plant growth promoting characteristic of HA with the inoculation of PGPB selected strains in the treatment of maize seeds. Thus, providing superior responses than in single applications of both in the initial maize development. To meet this purpose, we conducted isolated application of HA or PGPB inoculation of Burkholderia gladioli and Rhizobium cellulosilyticum, and the combined application of PGPB and HA for treatment of maize seeds. At the end of the experiment (45 days after germination), the plants were evaluated biometrically, nutritionally and a bacteria count was performed in plants using the Most Probable Number technique. The results showed that it is possible to combine the effects of HA with the inoculation of selected strains of PGPB, obtaining superior responses to the isolated application of both. Thus, the use of HA-based bio-stimulants in combination with PGPB is positive and complementary compared to inputs generally used in the treatment of maize seeds.

scholarly journals Genome-guided insights of tropical Bacillus strains efficient in maize growth promotion

2020 ◽  
Vol 96 (9) ◽  
Author(s):  
Camila Cristina Vieira Velloso ◽  
Christiane Abreu de Oliveira ◽  
Eliane Aparecida Gomes ◽  
Ubiraci Gomes de Paula Lana ◽  
Chainheny Gomes de Carvalho ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Dry Weight ◽  
Phenotypic Characterization ◽  
Plant Growth Promoting Bacteria ◽  
Maize Genotypes ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Bacillus Strains

ABSTRACT Plant growth promoting bacteria (PGPB) are an efficient and sustainable alternative to mitigate biotic and abiotic stresses in maize. This work aimed to sequence the genome of two Bacillus strains (B116 and B119) and to evaluate their plant growth-promoting (PGP) potential in vitro and their capacity to trigger specific responses in different maize genotypes. Analysis of the genomic sequences revealed the presence of genes related to PGP activities. Both strains were able to produce biofilm and exopolysaccharides, and solubilize phosphate. The strain B119 produced higher amounts of IAA-like molecules and phytase, whereas B116 was capable to produce more acid phosphatase. Maize seedlings inoculated with either strains were submitted to polyethylene glycol-induced osmotic stress and showed an increase of thicker roots, which resulted in a higher root dry weight. The inoculation also increased the total dry weight and modified the root morphology of 16 out of 21 maize genotypes, indicating that the bacteria triggered specific responses depending on plant genotype background. Maize root remodeling was related to growth promotion mechanisms found in genomic prediction and confirmed by in vitro analysis. Overall, the genomic and phenotypic characterization brought new insights to the mechanisms of PGP in tropical Bacillus.

Improved establishment and development of three cactus species inoculated withAzospirillum brasilensetransplanted into disturbed urban desert soil

1999 ◽  
Vol 45 (6) ◽  
pp. 441-451 ◽  
Author(s):  
Yoav Bashan ◽  
Adriana Rojas ◽  
M Esther Puente
Keyword(s):  
Soil Erosion ◽  
Plant Growth ◽  
Azospirillum Brasilense ◽  
Soil Reclamation ◽  
High Survival ◽  
Plant Growth Promoting Bacteria ◽  
Disturbed Areas ◽  
Small Roots ◽  
Plant Growth Promoting ◽  
Growth Promoting

Survival and development of cactus transplants in urban, disturbed areas of the desert near La Paz, Baja California Sur, Mexico, was monitored. Young plants of three species of pachycereid cacti (Pachycereus pringlei, Stenocereus thurberi, and Lophocereus schottii) inoculated with the plant growth promoting bacterium Azospirillum brasilense in an eroded area (a dirt road) had a high survival rate and developed more rapidly compared with uninoculated control plants during a 3.5-year period after transplantation. Soil erosion in the inoculated experimental area diminished. Small, but significant soil accumulated in association with the growth of cactus roots into the wind-deposited dust. One demonstrated mechanism for stabilizing dust was by the upward growth of small roots during the rainy season into the deposited dust. Azospirillum brasilense survived well in the rhizospheres of these cacti for 2 years, but not in root-free soil. This study demonstrated the feasibility of using bacterial inoculation of cacti to enhance their establishment in disturbed areas, with the potential to stabilize soil.Key words: Azospirillum, beneficial bacteria, cactus, plant inoculation, plant growth promoting bacteria, PGPR, soil erosion, soil reclamation.

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