scholarly journals Assessment of endophytic bacterial diversity in rose by high-throughput sequencing analysis

PLoS ONE ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. e0230924
Author(s):  
Ao-Nan Xia ◽  
Jun Liu ◽  
Da-Cheng Kang ◽  
Hai-Guang Zhang ◽  
Ru-Hua Zhang ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Sequencing Analysis

scholarly journals High-throughput sequencing analysis of bacterial diversity in raw and pasteurized goat milk

2019 ◽  
Author(s):  
Feng Huang ◽  
Siqi Liu ◽  
Xiaokang Zhou ◽  
Pengfei Wang ◽  
Rengchun He ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Bacterial Species ◽  
Goat Milk ◽  
Raw Milk ◽  
Sequencing Analysis ◽  
Quality And Safety ◽  
Microbial Composition ◽  
High Throughput Dna Sequencing

AbstractThe aim of this study was to evaluate the microbial composition of both raw and pasteurized goat milk using high-throughput DNA sequencing. This analysis revealed that the dominant phylum found in the raw milk was Proteobacteria, and the dominant genus was Kluyvera; Proteobacteria and Kluyvera constituted up to 67.66% and 28.85% of the total bacteria population, respectively. The microorganisms in goat milk predominantly consist of Gram-negative bacteria. Notably, Akkermansia and Faecalibacterium were identified in goat milk for the first time. In addition, the results also indicate that some bacteria in pasteurized goat milk may exist in a viable but nonculturable (VBNC) state. This study provides a theoretical basis that may aid the community in better understanding bacterial diversity in goat milk. The results of this study will help us to improve the quality and safety of goat milk.ImportanceThe microbial diversity in goat milk and pasteurized goat milk at different refrigeration stages was described. Several bacterial species that have not previously been reported in goat milk were identified, including many VBNC bacteria. The findings provided the necessary microbial information for quality and safety of goat milk and dairy products.

scholarly journals Characterization of oral bacterial diversity of irradiated patients by high-throughput sequencing

2013 ◽  
Vol 5 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Yue-Jian Hu ◽  
Qian Wang ◽  
Yun-Tao Jiang ◽  
Rui Ma ◽  
Wen-Wei Xia ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
High Throughput ◽  
High Throughput Sequencing

scholarly journals High-Throughput Sequencing Analysis of the Actinobacterial Spatial Diversity in Moonmilk Deposits

Antibiotics ◽  
2018 ◽  
Vol 7 (2) ◽  
pp. 27 ◽  
Author(s):  
Marta Maciejewska ◽  
Magdalena Całusińska ◽  
Luc Cornet ◽  
Delphine Adam ◽  
Igor Pessi ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Spatial Diversity ◽  
Sequencing Analysis

In vitro fertilization and preimplantation genetic testing methods in infertility treatment of a woman with karyotype 46,XX,ins(13;4)(q34;p14p15.3),inv(4)(p14q12). Case report

GYNECOLOGY ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. 441-444
Author(s):  
Zhanna I. Glinkina ◽  
Elena V. Kulakova ◽  
Elena G. Lebedeva ◽  
Varvara S. Kuzmicheva ◽  
Nataliya P. Makarova
Keyword(s):  
Genetic Testing ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Chromosomal Abnormalities ◽  
Reproductive Technologies ◽  
Infertility Treatment ◽  
Preimplantation Embryos ◽  
Sequencing Analysis ◽  
Preimplantation Genetic Testing ◽  
And Inversion

The frequency of structural chromosomal transpositions can range from 1.8 to 8% among patients with reproductive disorders. There are several types of the rarest chromosomal abnormalities: insertion (insertion of a chromosomal region) and inversion (rotation of a chromosome region). This article describes a clinical case of the infertility treatment using assisted reproductive technologies in a woman with a rare chromosomal abnormality: simultaneous insertion and inversion of chromosomes 46, XX, ins (13;4)(q34;p14p15.3), inv(4)(p14q12). The structure and frequency of chromosomal aberrations were determined by high-throughput sequencing in preimplantation embryos. The result of the sequencing analysis showed that unbalanced variants for a known pathology were detected in 9 (56.3%) out of 16 observations, while in 6 (37%) only for a pathology known in the karyotype and in 3 (19%) they were presented simultaneously with the pathology of other chromosomes or with mosaicism. According to the results of the study, in preimplantation embryos, where one of the parents had chromosomal abnormalities, in addition to unbalanced variants, there is aneuploidy of other chromosomes not involved in the known pathology. They are described in 3 (21%) out of 14 observations of all identified pathology. In this regard, patients with aberrations in the karyotype are recommended, whenever possible, to carry out preimplantation genetic testing of structural rearrangements by methods allowed to analyze all chromosomes simultaneously. For example, high-throughput sequencing on the Illumina platform may become an alternative for prenatal diagnostics, which is performed in fertile couples with high risk of having a child with hereditary or congenital disorders. In the case of detection of chromosomal changes in the fetus, patients are faced with a number of ethical issues related to the necessity for medical abortion, which may contradict their religious and moral convictions.

High-throughput sequencing analysis of endophytic fungal diversity in cynanchum sp.

2020 ◽  
Vol 134 ◽  
pp. 349-358
Author(s):  
W.-H. Chen ◽  
S.-J. Wu ◽  
X.-L. Sun ◽  
K.-M. Feng ◽  
K. Rahman ◽  
...  
Keyword(s):  
High Throughput ◽  
Fungal Diversity ◽  
High Throughput Sequencing ◽  
Sequencing Analysis
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