Interlaboratory comparison for the Filovirus Animal Nonclinical Group (FANG) anti-Ebola virus glycoprotein immunoglobulin G enzyme-linked immunosorbent assay

We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using partial recombinant nucleoproteins (rNP) of Reston Ebola virus (EBO-R) and Zaire Ebola virus (EBO-Z). We examined the reaction of 10 sera from cynomolgus macaques naturally infected with EBO-R to each of the partial rNP in the IgG ELISA. All the sera reacted to the C-terminal halves of the rNP of both EBO-R and EBO-Z. Most of the sera reacted to the RΔC (amino acid (aa) 360–739), and RΔ6 (aa 451–551) and/or RΔ8 (aa 631–739) at a higher dilution than to the corresponding truncated rNPs of EBO-Z. The results indicate that this IgG ELISA is useful for detecting EBO-R specific antibody, and may have a potential to discriminate EBO-R infection from other subtypes.

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Evaluation of the INOVA Diagnostics Enzyme-Linked Immunosorbent Assay Kits for Measuring Serum Immunoglobulin G (IgG) and IgA to Deamidated Gliadin Peptides

Clinical and Vaccine Immunology ◽

10.1128/cvi.13.1.150-151.2006 ◽

2006 ◽

Vol 13 (1) ◽

pp. 150-151 ◽

Cited By ~ 44

Author(s):

Harry E. Prince

Keyword(s):

Celiac Disease ◽

Immunosorbent Assay ◽

Immunoglobulin G ◽

Immunoglobulin A ◽

Serum Immunoglobulin ◽

Enzyme Linked Immunosorbent Assay ◽

Gliadin Antibodies ◽

Gliadin Peptides ◽

Disease Specific ◽

Inova Diagnostics

ABSTRACT New assays for antibodies to deamidated gliadin peptides (DGP) expressing celiac disease-specific epitopes were evaluated using 154 sera previously tested for endomysial immunoglobulin A (IgA) (EMA), transglutaminase IgA (TGA), and conventional gliadin antibodies. DGP antibody results showed 97% concordance with EMA and TGA results. Of 56 sera negative for EMA and TGA but positive for conventional gliadin antibodies, 54 (96%) were negative for DGP antibodies.

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Analysis of Specific Immunoglobulin G Subclass Antibodies for Serological Diagnosis of Echinococcosis by a Standard Enzyme-Linked Immunosorbent Assay

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.5.5.613-616.1998 ◽

1998 ◽

Vol 5 (5) ◽

pp. 613-616 ◽

Cited By ~ 21

Author(s):

Felix Grimm ◽

Friedrich E. Maly ◽

Jian Lü ◽

Roberto Llano

Keyword(s):

Healthy Volunteers ◽

Immunosorbent Assay ◽

Immunoglobulin G ◽

Serological Diagnosis ◽

Enzyme Linked Immunosorbent Assay ◽

Parasitic Infections ◽

Igg Subclass ◽

Specific Antibodies ◽

Specific Igg4 ◽

Antibody Levels

ABSTRACT The potential roles of specific antibodies of the different immunoglobulin G (IgG) subclasses in the serological diagnosis of cystic echinococcosis (CE) and alveolar echinococcosis (AE) were investigated by an enzyme-linked immunosorbent assay based on hydatid fluid as antigen. Specific antibodies of subclass 1 were found to be of major importance. In sera collected at the time of diagnosis (i.e., before any therapeutic intervention was initiated) they could be demonstrated in 14 of 15 sera from patients with CE and in all 12 sera from patients with AE. The most discriminatory and the most specific antibodies found in this study belonged to IgG subclass 4. Only one false-positive reaction was observed with 253 sera from healthy volunteers, and no cross-reactions occurred in 80 sera from patients with different parasitic infections. Specific IgG4 antibodies could be demonstrated in 61.0 to 66.7% (CE) or 47.6 to 66.7% (AE) of the cases. Antibody levels of IgG subclass 2 were elevated only moderately, and subclass 3 antibodies were detected in a few cases only. In addition, nonspecific reactions in sera of healthy volunteers or patients with other parasitic infections could partially be attributed to antibodies of subclasses 2 and 3.

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Hemagglutinin-specific antibody responses in immunoglobulin G, A, and M isotypes as measured by enzyme-linked immunosorbent assay after primary or secondary infection of humans with influenza A virus.

Infection and Immunity ◽

10.1128/iai.41.2.540-545.1983 ◽

1983 ◽

Vol 41 (2) ◽

pp. 540-545 ◽

Cited By ~ 49

Author(s):

D B Burlington ◽

M L Clements ◽

G Meiklejohn ◽

M Phelan ◽

B R Murphy

Keyword(s):

Influenza A Virus ◽

Immunosorbent Assay ◽

Specific Antibody ◽

Immunoglobulin G ◽

Influenza A ◽

Secondary Infection ◽

Antibody Responses ◽

Enzyme Linked Immunosorbent Assay

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Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms

Journal of Allergy and Clinical Immunology ◽

10.1016/0091-6749(81)90168-8 ◽

1981 ◽

Vol 68 (2) ◽

pp. 112-118 ◽

Cited By ~ 19

Author(s):

J.Andrew Grant ◽

Randall M. Goldblum ◽

Richard Rahr ◽

David O. Thueson ◽

Jafar Farnam ◽

...

Keyword(s):

Immunosorbent Assay ◽

Immunoglobulin G ◽

Enzyme Linked Immunosorbent Assay

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Recombinant Diabody-Based Immunocapture Enzyme-Linked Immunosorbent Assay for Quantification of Rabies Virus Glycoprotein

Clinical and Vaccine Immunology ◽

10.1128/cvi.00204-10 ◽

2010 ◽

Vol 17 (8) ◽

pp. 1261-1268 ◽

Cited By ~ 20

Author(s):

Sridevi V. Nimmagadda ◽

Shukra M. Aavula ◽

Neelakantam Biradhar ◽

Varaprasada Sankarasetty Rao ◽

Rajalakshmi Shanmugham ◽

...

Keyword(s):

Rabies Virus ◽

Immunosorbent Assay ◽

Human Monoclonal Antibody ◽

Expression System ◽

Nitrilotriacetic Acid ◽

Human Rabies ◽

Enzyme Linked Immunosorbent Assay ◽

Rabies Virus Glycoprotein ◽

Virus Glycoprotein ◽

Overlap Extension

ABSTRACT The potency of rabies vaccines, determined using the NIH mouse protection test, can be directly correlated to the amount of rabies virus glycoprotein (RV GP) present in the vaccine. In an effort to develop a simple and sensitive enzyme-linked immunosorbent assay (ELISA) using recombinant diabody for quantification of RV GP, the variable heavy (VH) and light chain (VL) domains of an RV GP-specific human monoclonal antibody (MAb) secreted by a human × mouse heterohybridoma (human MAb R16E5) was amplified, linked using splicing by overlap extension PCR (SOE PCR), and expressed as a recombinant diabody (D06) in the pET28a bacterial expression system. The diabody D06 was purified by immobilized metal affinity chromatography on a nickel-nitrilotriacetic acid (NTA) agarose column and characterized. The purified diabody was used in combination with a well-characterized RV GP-specific mouse MAb, M5B4, to develop an immunocapture ELISA (IC-ELISA) for the quantification of RV GP in human rabies vaccine preparations. The maximum detection limit of the IC-ELISA using the M5B4-D06 combination was up to 31.25 ng/ml of RV GP. The specificity of the diabody was established by its nonreactivity toward other human viral antigens as determined by ELISA and toward RV GP as determined by immunoblot transfer assay and competitive ELISA with the parent human MAb R16E5 and MAb M5B4. The adjusted r 2 value obtained by the regression through the origin model was 0.902, and the equation for predicted potency values for M5B4-D06-based IC-ELISA and MAb M5B4 IC-ELISA were 0.5651x and 0.8044x, respectively, where x is the estimate of RV GP from the IC-ELISA in micrograms. Analysis of variance (ANOVA) results showed the estimates of the two methods differed significantly (P < 0.001), while the predicted potencies by the two tests did not differ significantly (P > 0.05). The IC-ELISA can be readily adapted to measure the RV GP content in purified antigen, and a vaccine can be formulated based on the estimated GP.

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Enzyme-linked immunosorbent assay for the detection of anti-Giardia specific immunoglobulin G in filter paper blood samples

Transactions of the Royal Society of Tropical Medicine and Hygiene ◽

10.1016/0035-9203(93)90412-j ◽

1993 ◽

Vol 87 (1) ◽

pp. 36-38 ◽

Cited By ~ 12

Author(s):

M.H. Al-Tukhi ◽

J.P. Ackers ◽

M.N. Al-Ahdal ◽

W. Peters

Keyword(s):

Immunosorbent Assay ◽

Immunoglobulin G ◽

Filter Paper ◽

Enzyme Linked Immunosorbent Assay ◽

Blood Samples ◽

Specific Immunoglobulin

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Assignment of Additional Anticapsular Antibody Concentrations to the Neisseria meningitidis Group A, C, Y, and W-135 Meningococcal Standard Reference Serum CDC1992

Clinical and Vaccine Immunology ◽

10.1128/cdli.9.3.725-726.2002 ◽

2002 ◽

Vol 9 (3) ◽

pp. 725-726 ◽

Cited By ~ 4

Author(s):

Cheryl M. Elie ◽

Patricia K. Holder ◽

Sandra Romero-Steiner ◽

George M. Carlone

Keyword(s):

Quality Control ◽

Disease Control ◽

Neisseria Meningitidis ◽

Immunosorbent Assay ◽

Immunoglobulin G ◽

Enzyme Linked Immunosorbent Assay ◽

Control And Prevention ◽

Group A ◽

Reference Serum ◽

Centers For Disease Control

ABSTRACT We assigned additional enzyme-linked immunosorbent assay antibody concentrations (immunoglobulin G [IgG], IgM, and IgA, and total) to the Neisseria meningitidis standard reference serum CDC1992 for groups Y and W-135 to 12 Centers for Disease Control and Prevention quality control sera. These assignments will supplement previous assignments and will aid in the evaluation of present and developing vaccines.

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