scholarly journals High resolution ultrasound imaging for repeated measure of wound tissue morphometry, biomechanics and hemodynamics under fetal, adult and diabetic conditions

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0241831
Author(s):  
Surya C. Gnyawali ◽  
Mithun Sinha ◽  
Mohamed S. El Masry ◽  
Brian Wulff ◽  
Subhadip Ghatak ◽  
...  
Keyword(s):  
Wound Healing ◽  
High Resolution ◽  
Ultrasound Imaging ◽  
High Frequency ◽  
Normal Skin ◽  
Repeated Measure ◽  
Non Invasive ◽  
Severe Strain ◽  
Inflammatory Phase ◽  
Wound Tissue

Non-invasive, repeated interrogation of the same wound is necessary to understand the tissue repair continuum. In this work, we sought to test the significance of non-invasive high-frequency high-resolution ultrasound technology for such interrogation. High-frequency high-resolution ultrasound imaging was employed to investigate wound healing under fetal and adult conditions. Quantitative tissue cellularity and elastic strain was obtained for visualization of unresolved inflammation using Vevo strain software. Hemodynamic properties of the blood flow in the artery supplying the wound-site were studied using color Doppler flow imaging. Non-invasive monitoring of fetal and adult wound healing provided unprecedented biomechanical and functional insight. Fetal wounds showed highly accelerated closure with transient perturbation of wound tissue cellularity. Fetal hemodynamics was unique in that sharp fall in arterial pulse pressure (APP) which was rapidly restored within 48h post-wounding. In adults, APP transiently increased post-wounding before returning to the pre-wounding levels by d10 post-wounding. The pattern of change in the elasticity of wound-edge tissue of diabetics was strikingly different. Severe strain acquired during the early inflammatory phase persisted with a slower recovery of elasticity compared to that of the non-diabetic group. Wound bed of adult diabetic mice (db/db) showed persistent hypercellularity compared to littermate controls (db/+) indicative of prolonged inflammation. Normal skin strain of db/+ and db/db were asynchronous. In db/db, severe strain acquired during the early inflammatory phase persisted with a slower recovery of elasticity compared to that of non-diabetics. This study showcases a versatile clinically relevant imaging platform suitable for real-time analyses of functional wound healing.

scholarly journals High-Resolution Harmonics Ultrasound Imaging for Non-Invasive Characterization of Wound Healing in a Pre-Clinical Swine Model

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0122327 ◽  
Author(s):  
Surya C. Gnyawali ◽  
Kasturi G. Barki ◽  
Shomita S. Mathew-Steiner ◽  
Sriteja Dixith ◽  
Daniel Vanzant ◽  
...  
Keyword(s):  
Wound Healing ◽  
High Resolution ◽  
Ultrasound Imaging ◽  
Swine Model ◽  
Non Invasive

scholarly journals Ultrasound imaging of apoptosis: high-resolution non-invasive monitoring of programmed cell death in vitro, in situ and in vivo

1999 ◽  
Vol 81 (3) ◽  
pp. 520-527 ◽  
Author(s):  
G J Czarnota ◽  
M C Kolios ◽  
J Abraham ◽  
M Portnoy ◽  
F P Ottensmeyer ◽  
...  
Keyword(s):  
Cell Death ◽  
High Resolution ◽  
Programmed Cell Death ◽  
Ultrasound Imaging ◽  
Invasive Monitoring ◽  
Non Invasive

Non-invasive high-resolution acoustic microscopy technique using embedded nanostructures

2007 ◽  
Vol 1019 ◽  
Author(s):  
Daniel Wulin ◽  
Shriram Ramanathan
Keyword(s):  
High Resolution ◽  
Acoustic Waves ◽  
High Frequency ◽  
Acoustic Microscopy ◽  
Biological Applications ◽  
Acoustic Oscillations ◽  
Non Invasive ◽  
Microscopy Technique ◽  
First Time

AbstractAn opto-acoustic system capable of operating at frequencies greater than 1 GHz with novel biological applications is proposed for the first time. Metallic spheres with radii on the order of hundreds of nanometers dispersed inside a bio-matrix can be used to generate in-situ ultra-high frequency acoustic waves whose normal mode frequencies can be calculated using Lamb's theory for acoustic oscillations of elastic spheres. The frequency and amplitude of the resulting acoustic waves can be related to the physical properties of the metallic spheres and the surrounding bio-matrix: the acoustic waves produced by the metallic spheres are well-suited to high resolution acoustic imaging. We anticipate that our approach will open up new nanoscale techniques to study cells non-invasively.

scholarly journals Accelerated complete human skin architecture restoration after wounding by nanogenerator-driven electrostimulation

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Aiping Liu ◽  
Yin Long ◽  
Jun Li ◽  
Long Gu ◽  
Aos Karim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Wound Closure ◽  
Normal Skin ◽  
Xenograft Model ◽  
Clinical Use ◽  
Inflammatory Phase ◽  
Complete Restoration ◽  
Skin Xenograft

Abstract Background Electrostimulation (ES) therapy for wound healing is limited in clinical use due to barriers such as cumbersome equipment and intermittent delivery of therapy. Methods We adapted a human skin xenograft model that can be used to directly examine the nanogenerator-driven ES (NG-ES) effects on human skin in vivo—an essential translational step toward clinical application of the NG-ES technique for wound healing. Results We show that NG-ES leads to rapid wound closure with complete restoration of normal skin architecture within 7 days compared to more than 30 days in the literature. NG-ES accelerates the inflammatory phase of wound healing with more rapid resolution of neutrophils and macrophages and enhances wound bed perfusion with more robust neovascularization. Conclusion Our results support the translational evaluation and optimization of the NG-ES technology to deliver convenient, efficient wound healing therapy for use in human wounds. Graphic abstract

scholarly journals Overview of Ultrasound Imaging Applications in Dermatology

2021 ◽  
pp. 120347542199932
Author(s):  
Nouf Almuhanna ◽  
Ximena Wortsman ◽  
Iris Wohlmuth-Wieser ◽  
Misaki Kinoshita-Ise ◽  
Raed Alhusayen
Keyword(s):  
Ultrasound Imaging ◽  
Clinical Examination ◽  
High Frequency ◽  
Accurate Diagnosis ◽  
Handheld Devices ◽  
Ultra High Frequency ◽  
Dermatological Diseases ◽  
Diagnosis And Management ◽  
Non Invasive ◽  
Technological Advances

Complete visualization of lesions is critical for the accurate diagnosis and management of dermatological diseases. Currently, the most readily available technologies used by dermatologists include dermoscopy and photography. Nevertheless, ultrasound has emerged as a useful non-invasive modality in dermatology, which can be added to the clinical examination supporting an early and more accurate diagnosis. Moreover, there are significant technological advances in recent years, such as the development of handheld devices and ultra-high frequency probes that have expanded the integration of ultrasound into daily dermatology practice. In this article, we reviewed the most common applications of ultrasound in the field of dermatology.

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