scholarly journals High-Resolution Harmonics Ultrasound Imaging for Non-Invasive Characterization of Wound Healing in a Pre-Clinical Swine Model

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0122327 ◽  
Author(s):  
Surya C. Gnyawali ◽  
Kasturi G. Barki ◽  
Shomita S. Mathew-Steiner ◽  
Sriteja Dixith ◽  
Daniel Vanzant ◽  
...  
Keyword(s):  
Wound Healing ◽  
High Resolution ◽  
Ultrasound Imaging ◽  
Swine Model ◽  
Non Invasive

scholarly journals High resolution ultrasound imaging for repeated measure of wound tissue morphometry, biomechanics and hemodynamics under fetal, adult and diabetic conditions

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0241831
Author(s):  
Surya C. Gnyawali ◽  
Mithun Sinha ◽  
Mohamed S. El Masry ◽  
Brian Wulff ◽  
Subhadip Ghatak ◽  
...  
Keyword(s):  
Wound Healing ◽  
High Resolution ◽  
Ultrasound Imaging ◽  
High Frequency ◽  
Normal Skin ◽  
Repeated Measure ◽  
Non Invasive ◽  
Severe Strain ◽  
Inflammatory Phase ◽  
Wound Tissue

Non-invasive, repeated interrogation of the same wound is necessary to understand the tissue repair continuum. In this work, we sought to test the significance of non-invasive high-frequency high-resolution ultrasound technology for such interrogation. High-frequency high-resolution ultrasound imaging was employed to investigate wound healing under fetal and adult conditions. Quantitative tissue cellularity and elastic strain was obtained for visualization of unresolved inflammation using Vevo strain software. Hemodynamic properties of the blood flow in the artery supplying the wound-site were studied using color Doppler flow imaging. Non-invasive monitoring of fetal and adult wound healing provided unprecedented biomechanical and functional insight. Fetal wounds showed highly accelerated closure with transient perturbation of wound tissue cellularity. Fetal hemodynamics was unique in that sharp fall in arterial pulse pressure (APP) which was rapidly restored within 48h post-wounding. In adults, APP transiently increased post-wounding before returning to the pre-wounding levels by d10 post-wounding. The pattern of change in the elasticity of wound-edge tissue of diabetics was strikingly different. Severe strain acquired during the early inflammatory phase persisted with a slower recovery of elasticity compared to that of the non-diabetic group. Wound bed of adult diabetic mice (db/db) showed persistent hypercellularity compared to littermate controls (db/+) indicative of prolonged inflammation. Normal skin strain of db/+ and db/db were asynchronous. In db/db, severe strain acquired during the early inflammatory phase persisted with a slower recovery of elasticity compared to that of non-diabetics. This study showcases a versatile clinically relevant imaging platform suitable for real-time analyses of functional wound healing.

scholarly journals Ultrasound imaging of apoptosis: high-resolution non-invasive monitoring of programmed cell death in vitro, in situ and in vivo

1999 ◽  
Vol 81 (3) ◽  
pp. 520-527 ◽  
Author(s):  
G J Czarnota ◽  
M C Kolios ◽  
J Abraham ◽  
M Portnoy ◽  
F P Ottensmeyer ◽  
...  
Keyword(s):  
Cell Death ◽  
High Resolution ◽  
Programmed Cell Death ◽  
Ultrasound Imaging ◽  
Invasive Monitoring ◽  
Non Invasive

scholarly journals Noninvasive, Quantitative, Spatiotemporal Characterization of Mineralization in Three-Dimensional Collagen Hydrogels Using High-Resolution Spectral Ultrasound Imaging

2012 ◽  
Vol 18 (12) ◽  
pp. 935-946 ◽  
Author(s):  
Madhu Gudur ◽  
Rameshwar R. Rao ◽  
Yi-Sing Hsiao ◽  
Alexis W. Peterson ◽  
Cheri X. Deng ◽  
...  
Keyword(s):  
High Resolution ◽  
Ultrasound Imaging ◽  
Three Dimensional ◽  
Collagen Hydrogels

scholarly journals Optimised Extraction of Archaeological Features from Full 3-D GPR Data

2021 ◽  
Vol 11 (18) ◽  
pp. 8517
Author(s):  
Emanuele Forte ◽  
Arianna Mocnik ◽  
Patrizia Basso ◽  
Giulia Casagrande ◽  
Davide Martinucci ◽  
...  
Keyword(s):  
High Resolution ◽  
Ground Penetrating Radar ◽  
Archaeological Site ◽  
Complex Structures ◽  
Non Invasive ◽  
Digital Terrain ◽  
Final Interpretation ◽  
Ground Penetrating ◽  
Terrain Modelling

The use of non-invasive methodologies is becoming essential for archaeological research, and ground penetrating radar is one of the most important techniques to obtain high resolution information. In this paper we present the analysis of a full 3-D GPR dataset integrated with a high-resolution photogrammetric survey acquired in a Roman archaeological site located in Aquileia (Northeast Italy) within the partially excavated area known as “Fondo Pasqualis”. We evaluated the importance of dense and accurate data collection and of processing of the GPR signal for characterization of the archaeological features. We further discuss the parametrization and the applicability of GPR attributes, in particular amplitude-based and coherence attributes, to better identify and characterise the archaeological buried targets. Furthermore, autopicking procedures for isosurfaces mapping were critically evaluated with the objective of detecting complex structures. The final interpretation of all the GPR features, with the support of digital terrain modelling and orthophotos from unmanned aerial vehicles, guided the archaeologists to open and excavate newly selected areas, which revealed interesting structures and contributed to the understanding of the historical events that characterized the Aquileia city.

Skeletal elements of crinoid echinoderms: High-resolution structural and microanalytical results

1983 ◽  
Vol 41 ◽  
pp. 194-195
Author(s):  
D. F. Blake ◽  
L. F. Allard ◽  
D. R. Peacor
Keyword(s):  
High Resolution ◽  
Marine Invertebrates ◽  
Sea Urchins ◽  
Structural Features ◽  
Sea Stars ◽  
High Resolution Tem ◽  
Relationship Of ◽  
The Relationship ◽  
Insight Into

Echinodermata is a phylum of marine invertebrates which has been extant since Cambrian time (c.a. 500 m.y. before the present). Modern examples of echinoderms include sea urchins, sea stars, and sea lilies (crinoids). The endoskeletons of echinoderms are composed of plates or ossicles (Fig. 1) which are with few exceptions, porous, single crystals of high-magnesian calcite. Despite their single crystal nature, fracture surfaces do not exhibit the near-perfect {10.4} cleavage characteristic of inorganic calcite. This paradoxical mix of biogenic and inorganic features has prompted much recent work on echinoderm skeletal crystallography. Furthermore, fossil echinoderm hard parts comprise a volumetrically significant portion of some marine limestones sequences. The ultrastructural and microchemical characterization of modern skeletal material should lend insight into: 1). The nature of the biogenic processes involved, for example, the relationship of Mg heterogeneity to morphological and structural features in modern echinoderm material, and 2). The nature of the diagenetic changes undergone by their ancient, fossilized counterparts. In this study, high resolution TEM (HRTEM), high voltage TEM (HVTEM), and STEM microanalysis are used to characterize tha ultrastructural and microchemical composition of skeletal elements of the modern crinoid Neocrinus blakei.

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