scholarly journals Relationships of capsular polysaccharides belonging to Campylobacter jejuni HS1 serotype complex

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0247305
Author(s):  
Mario A. Monteiro ◽  
Yu-Han Chen ◽  
Zuchao Ma ◽  
Cheryl P. Ewing ◽  
Nooraisyah Mohamad Nor ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Type Strain ◽  
Teichoic Acid ◽  
Cross Reactivity ◽  
Capsular Polysaccharides ◽  
Common Component ◽  
Biosynthesis Gene ◽  
Capsule Locus ◽  
Common Serotypes ◽  
The Common

The Campylobacter jejuni capsule type HS1 complex is one of the most common serotypes identified worldwide, and consists of strains typing as HS1, HS1/44, HS44 and HS1/8. The capsule structure of the HS1 type strain was shown previously to be composed of teichoic-acid like glycerol-galactosyl phosphate repeats [4-)-α-D-Galp-(1–2)-Gro-(1-P-] with non-stoichiometric fructose branches at the C2 and C3 of Gal and non-stoichiometric methyl phosphoramidate (MeOPN) modifications on the C3 of the fructose. Here, we demonstrate that the capsule of an HS1/44 strain is identical to that of the type strain of HS1, and the capsule of HS1/8 is also identical to HS1, except for an additional site of MeOPN modification at C6 of Gal. The DNA sequence of the capsule locus of an HS44 strain included an insertion of 10 genes, and the strain expressed two capsules, one identical to the HS1 type strain, but with no fructose branches, and another composed of heptoses and MeOPN. We also characterize a HS1 capsule biosynthesis gene, HS1.08, as a fructose transferase responsible for the attachment of the β-D-fructofuranoses residues at C2 and C3 of the Gal unit. In summary, the common component of all members of the HS1 complex is the teichoic-acid like backbone that is likely responsible for the observed sero-cross reactivity.

scholarly journals Characterization of Two Campylobacter jejuni Strains for Use in Volunteer Experimental-Infection Studies

2008 ◽  
Vol 76 (12) ◽  
pp. 5655-5667 ◽  
Author(s):  
Frédéric Poly ◽  
Timothy D. Read ◽  
Yu-Han Chen ◽  
Mario A. Monteiro ◽  
Oralak Serichantalergs ◽  
...  
Keyword(s):  
Southeast Asia ◽  
Campylobacter Jejuni ◽  
Experimental Infection ◽  
Type Strain ◽  
Molecular Mimicry ◽  
Clinical Isolates ◽  
Capsule Locus ◽  
High Degree ◽  
Degree Of Similarity

ABSTRACT The development of vaccines against Campylobacter jejuni would be facilitated by the ability to perform phase II challenge studies. However, molecular mimicry of the lipooligosaccharide (LOS) of most C. jejuni strains with human gangliosides presents safety concerns about the development of Guillain-Barré syndrome. Clinical isolates of C. jejuni that appeared to lack genes for the synthesis of ganglioside mimics were identified by DNA probe analyses. Two clinical isolates from Southeast Asia (strains BH-01-0142 and CG8421) were determined to express the LOS type containing N-acetyl quinovosamine. No ganglioside structures were observed to be present in the LOSs of these strains, and pyrosequence analyses of the genomes of both strains confirmed the absence of genes involved in ganglioside mimicry. The capsule polysaccharide (CPS) of BH-01-0142 was determined to be composed of galactose (Gal), 6-deoxy-ido-heptose, and, in smaller amounts, d-glycero-d-ido-heptose, and the CPS of CG8421 was observed to contain Gal, 6-deoxy-altro-heptose, N-acetyl-glucosamine, and minor amounts of 6-deoxy-3-O-Me-altro-heptose. Both CPSs were shown to carry O-methyl-phosphoramidate. The two genomes contained strain-specific zones, some of which could be traced to a plasmid origin, and both contained a large chromosomal insertion related to the CJEI3 element of C. jejuni RM1221. The genomes of both strains shared a high degree of similarity to each other and, with the exception of the capsule locus of CG8421, to the type strain of the HS3 serotype, TGH9011.

scholarly journals Subtyping Salmonella isolated from pet dogs with multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs)

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Cheng Yang ◽  
Wangfeng Shao ◽  
Lingling Wei ◽  
Lingxiao Chen ◽  
Aihua Zhu ◽  
...  
Keyword(s):  
Multilocus Sequence Typing ◽  
Sequence Type ◽  
Jiangsu Province ◽  
Zoonotic Pathogen ◽  
Recognition Ability ◽  
Prevention And Treatment ◽  
Common Serotypes ◽  
The Common ◽  
Different Sources ◽  
Positive Effect

AbstractSalmonella, as a zoonotic pathogen, has attracted widespread attention worldwide, especially in the transmission between household pets and humans. Therefore, we investigated the epidemic distribution of dog Salmonella from pet hospitals and breeding base in Xuzhou, Jiangsu Province, China, and used multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs) to subtype Salmonella isolates. From April 2018 to November 2019, a total of 469 samples were collected from pet hospitals and breeding base, including 339 dog samples and 60 cat samples. S. Kentucky (40.74%) was the most prevalent serotype, but other, such as S. Typhimurium (18.52%) and S. Indiana (18.52%), were also widespread. Eight different sequence type (ST) patterns were identified by MLST and ST198 was the highest proportion of these isolates. CRISPRs analysis showed that 9 different Kentucky CRISPR types (KCTs) was identified from ST198. 48 spacers including 29 (6 News) for CRISPR1 and 19 (4 News) for CRISPR2 that proved the polymorphic of Salmonella genes in samples from different sources. The analysis demonstrated that the common serotypes were widely present in pet hosts in the same area. This analysis shows that CRISPR genes have better recognition ability in the same serotype, which has a positive effect on the traceability of Salmonella and the prevention and treatment of salmonellosis.

scholarly journals Examination of the Efficacy and Cross-Reactivity of a Novel Polyclonal Antibody Targeting the Disintegrin Domain in SVMPs to Neutralize Snake Venom

Toxins ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 254
Author(s):  
Shelby S. Szteiter ◽  
Ilse N. Diego ◽  
Jonathan Ortegon ◽  
Eliana Salinas ◽  
Abcde Cirilo ◽  
...  
Keyword(s):  
Snake Venom ◽  
Polyclonal Antibody ◽  
Polyclonal Antibodies ◽  
Cross Reactivity ◽  
Snake Envenomation ◽  
Disintegrin Domain ◽  
The Common ◽  
Neutralization Ability ◽  
New Strategies

Snake envenomation can result in hemorrhage, local necrosis, swelling, and if not treated properly can lead to adverse systemic effects such as coagulopathy, nephrotoxicity, neurotoxicity, and cardiotoxicity, which can result in death. As such, snake venom metalloproteinases (SVMPs) and disintegrins are two toxic components that contribute to hemorrhage and interfere with the hemostatic system. Administration of a commercial antivenom is the common antidote to treat snake envenomation, but the high-cost, lack of efficacy, side effects, and limited availability, necessitates the development of new strategies and approaches for therapeutic treatments. Herein, we describe the neutralization ability of anti-disintegrin polyclonal antibody on the activities of isolated disintegrins, P-II/P-III SVMPs, and crude venoms. Our results show disintegrin activity on platelet aggregation in whole blood and the migration of the SK-Mel-28 cells that can be neutralized with anti-disintegrin polyclonal antibody. We characterized a SVMP and found that anti-disintegrin was also able to inhibit its activity in an in vitro proteolytic assay. Moreover, we found that anti-disintegrin could neutralize the proteolytic and hemorrhagic activities from crude Crotalus atrox venom. Our results suggest that anti-disintegrin polyclonal antibodies have the potential for a targeted approach to neutralize SVMPs in the treatment of snakebite envenomations.

Time-Varying Uncertainty of the Federal Reserve's Output Gap Estimate

2021 ◽  
pp. 1-38
Author(s):  
Travis J. Berge
Keyword(s):  
Aggregate Demand ◽  
Stochastic Volatility Model ◽  
Output Gap ◽  
Time Varying ◽  
Common Component ◽  
Macroeconomic Variables ◽  
Macroeconomic Uncertainty ◽  
Volatility Model ◽  
The Common ◽  
Gap Estimate

Abstract A factor stochastic volatility model estimates the common component to output gap estimates produced by the staff of the Federal Reserve, its time-varying volatility, and time-varying, horizon-specific forecast uncertainty. The output gap estimates are uncertain even well after the fact. Nevertheless, the common component is clearly procyclical, and positive innovations to the common component produce movements in macroeconomic variables consistent with an increase in aggregate demand. Heightened macroeconomic uncertainty, as measured by the common component's volatility, leads to persistently negative economic responses.

Implant Infections Due to Enterococci: Role of Capsular Polysaccharides and Biofilm

2005 ◽  
Vol 28 (11) ◽  
pp. 1079-1090 ◽  
Author(s):  
F. Fabretti ◽  
J. Huebner
Keyword(s):  
Antimicrobial Agents ◽  
Capsular Polysaccharide ◽  
Female Genital Tract ◽  
Teichoic Acid ◽  
Capsular Polysaccharides ◽  
Human Pathogens ◽  
Artificial Joints ◽  
Artificial Hearts ◽  
Intravascular Catheters ◽  
Enterococcal Infections

Enterococci are natural inhabitants of the gastrointestinal tract and of the female genital tract of humans and many animals. In recent years, enterococci have been increasingly recognized as important human pathogens causing infections associated with medical devices. Their resistance to most antimicrobial agents and their ability to form biofilm has contributed to the increasing incidence of nosocomial enterococcal infections. Enterococci possess a capsular polysaccharide composed of a glycerol-teichoic acid-like molecule consisting of repeating units of 6-α-D-glucose-1-2-glycerol-3-PO4, substituted on carbon 2 with a α-2,1-linked molecule of glucose. Using both immunologic and genetic data E. faecalis can be assigned to specific serotypes based on capsular polysaccharides. Clinical examples of foreign-body infections due to enterococci are described, comprising infections of artificial joints, implanted intravascular catheters, artificial hearts and artificial valves, stents, liquor shunt devices, and intraocular infections. Methods to prevent and/or treat enterococcal infections are presented.

scholarly journals COMPARATIVE STUDY OF E-GOVERNMENT ENTERPRISE ARCHITECTURE BY PRIMARY ATTRIBUTES OF 3 ASIAN COUNTRIES

2013 ◽  
Vol 8 (2) ◽  
pp. 126
Author(s):  
Khakim Ghozali ◽  
Yudho Giri Sucahyo
Keyword(s):  
Information System ◽  
Enterprise Architecture ◽  
System Development ◽  
Asian Countries ◽  
Systems Architecture ◽  
Common Component ◽  
Business Area ◽  
The Common ◽  
Government Enterprise ◽  
Information Systems Architecture

By Waseda University International e-Government Ranking, announced in 2012 the state of Singapore and Korea ranks first and third in the implementation of e-government. Whereas Indonesia ranks 33[6]. This study aims to conduct a comparative analysis of the implementation of e-government Enterprise Architecture in Korea, Singapore and Indonesia. Stages of the research conducted is reviewing literature on TOGAF framework, define the attribute comparison, analysis of EA implementation in each country and do a comparison of the EA of both countries. Attributes used for comparison EA are business architecture, information systems architecture and technology architecture. Korea and Singapore divide business area into two business areas, namely public services and support services. Indonesia has tthree business area. Korea split by sector information system development while Singapore users group split based information system. Indonesia has 4 main application. Korea emphasizing mobile technology and the common component. While Singapore visible effort to increase the use of middleware. Indonesian technology solutions that enable information used accessed through various devices.

scholarly journals Mutational and transcriptional analysis of the Campylobacter jejuni flagellar biosynthesis gene flhB

Microbiology ◽  
2002 ◽  
Vol 148 (6) ◽  
pp. 1679-1685 ◽  
Author(s):  
Claudia Matz ◽  
Arnoud H. M. van Vliet ◽  
Julian M. Ketley ◽  
Charles W. Penn
Keyword(s):  
Campylobacter Jejuni ◽  
Transcriptional Analysis ◽  
Flagellar Biosynthesis ◽  
Biosynthesis Gene

scholarly journals Spasmolytic Action of Preparations and Compounds from Hofmeisteria schaffneri

2017 ◽  
Vol 12 (4) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Araceli Pérez-Vásquez ◽  
Guadalupe Ángeles-López ◽  
Isabel Rivero Cruz ◽  
Laura Flores-Bocanegra ◽  
Edelmira Linares ◽  
...  
Keyword(s):  
Essential Oil ◽  
Folk Medicine ◽  
Gastrointestinal Transit ◽  
Common Component ◽  
Relaxant Effect ◽  
Whole Plant ◽  
Spasmolytic Action ◽  
Control Procedures ◽  
The Common ◽  
Smooth Muscle Relaxant

Hofmeisteria schaffneri is used in Mexican folk medicine for treating painful gastric complaints. Therefore, in this paper the smooth muscle relaxant effect of the essential oil, and an infusion of the whole plant were evaluated using the gastrointestinal transit test in mice. The results revealed that both preparations at 316 mg/kg inhibited gastrointestinal transit by 47.5 and 52.1%, respectively. The common component of the infusion and essential oil was 8,9-epoxy-10-acetoxythymol angelate (2), which inhibited the gastrointestinal transit by 53.4% at a dose of 31.6 mg/kg. An HPLC-UV method was developed and validated to quantify 2. The chromatographic conditions were: A LiChrospher® 100 RP-18 column (250 × 4 mm i.d., 5 μm) with a mobile phase composed of CH3CN-H2O, in a gradient run at a flow rate of 0.6 mL/min, using a wavelength of 215 nm. The method was linear, precise, accurate, and showed excellent recovery. According to the results, compound 2 can be used as a marker for the quality control procedures of the crude drug of H. schaffneri.

A beam dynamics application based on the Common Component Architecture

2007 ◽  
Author(s):  
Douglas Ricker Dechow ◽  
Dan Tyler Abell ◽  
Peter Stoltz ◽  
Lois Curfman McInnes ◽  
Boyana Norris ◽  
...  
Keyword(s):  
Beam Dynamics ◽  
Common Component ◽  
Component Architecture ◽  
The Common ◽  
Common Component Architecture

Development of a Biotin-Avidin Mediated Immunoassay for Simultaneous Detection of Danofloxacin, Enrofloxacin and Ciprofloxacin in Milk

2013 ◽  
Vol 781-784 ◽  
pp. 1630-1636 ◽  
Author(s):  
Jie Biao Guo ◽  
Xuan Xin ◽  
Rui Min Zhong ◽  
Xing Ping Li
Keyword(s):  
Simultaneous Detection ◽  
Antigenic Site ◽  
Extraction Process ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Monoclonal Antibody ◽  
Common Structure ◽  
Coefficients Of Variation ◽  
Sample Extraction ◽  
The Common

To generate a group-specific monoclonal antibody (McAb) against danofloxacin (DANO), enrofloxacin (ENR) and ciprofloxacin (CIP), glutaraldehyde was used to link the presentative hapten of CIP to the immunogen and coating antigen, respectively, leaving the common structure of these 3 drugs exposed in both conjugates as a major antigenic site. Consequently, a McAb (6D3) with high cross-reactivity to this three antibiotics has been obtained by using hybridomas technique. In a biotin-avidin mediated enzyme-linked immunosorbent assay, the IC50values for DANO, ENR and CIP were 5.1, 4.5 and 4.2 ng mL-1, respectively. The ELISA was used for the detection of spiked DANO and ENR+CIP in milk. The recoveries ranged from 74.1 to 92.4% and coefficients of variation were in a range of 6.6-11.9%. The accuracies and sensitivity of the method were good for simultaneous analysis of the 3 drugs in milk after a simple sample extraction process.

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