Prevalence, serotype and antibiotic susceptibility of Group B Streptococcus isolated from pregnant women in Jakarta, Indonesia

Group B Streptococcus (GBS) is a bacterial pathogen which is a leading cause of neonatal infection. Currently, there are limited GBS data available from the Indonesian population. In this study, GBS colonization, serotype distribution and antimicrobial susceptibility profile of isolates were investigated among pregnant women in Jakarta, Indonesia. Demographics data, clinical characteristics and vaginal swabs were collected from 177 pregnant women (mean aged: 28.7 years old) at 29–40 weeks of gestation. Bacterial culture identification tests and latex agglutination were performed for GBS. Serotyping was done by conventional multiplex PCR and antibiotic susceptibility testing by broth microdilution. GBS colonization was found in 53 (30%) pregnant women. Serotype II was the most common serotype (30%) followed by serotype III (23%), Ia and IV (13% each), VI (8%), Ib and V (6% each), and one non-typeable strain. All isolates were susceptible to vancomycin, penicillin, ampicillin, cefotaxime, daptomycin and linezolid. The majority of GBS were resistant to tetracycline (89%) followed by clindamycin (21%), erythromycin (19%), and levofloxacin (6%). The serotype III was more resistant to erythromycin, clindamycin, and levofloxacin and these isolates were more likely to be multidrug resistant (6 out of 10) compared to other serotypes. This report provides demographics of GBS colonization and isolate characterization in pregnant women in Indonesia. The results may facilitate preventive strategies to reduce neonatal GBS infection and improve its treatment.

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W074 EFFECTIVENESS EVALUATION OF GROUP B STREPTOCOCCUS SCREENING IN PREGNANT WOMEN FOR PREVENTION OF NEONATAL INFECTION

International Journal of Gynecology & Obstetrics ◽

10.1016/s0020-7292(12)61799-8 ◽

2012 ◽

Vol 119 ◽

pp. S728-S729

Author(s):

M.Y. Kim ◽

M. Ki ◽

J.H. Yang ◽

Y.K. Lee ◽

G.H. An ◽

...

Keyword(s):

Pregnant Women ◽

Group B Streptococcus ◽

Neonatal Infection ◽

Effectiveness Evaluation ◽

Group B

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Antibiotic susceptibility pattern of group B streptococcal isolates from maternal genital tract

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20193035 ◽

2019 ◽

Vol 8 (7) ◽

pp. 2738

Author(s):

Vijayan Sharmila ◽

Thirunavukkarasu Arun Babu

Keyword(s):

Pregnant Women ◽

Antibiotic Susceptibility ◽

Group B Streptococcus ◽

Antibiotic Sensitivity ◽

Developed Countries ◽

Susceptibility Pattern ◽

Antibiotic Susceptibility Pattern ◽

Sensitivity Pattern ◽

Prevention And Treatment ◽

Group B

Background: Group B streptococcus (GBS) is one of the important cause of early onset neonatal sepsis in developed countries leading to increased neonatal morbidity and mortality. Penicillin and Ampicillin are the drugs of choice for prevention of GBS infections. Antibiotic resistance amongst GBS isolates is an emerging health problem affecting neonates. Hence, this study was performed to determine the antibiotic susceptibility pattern of Group B Streptococcus (GBS) in a population of pregnant women.Methods: A prospective study was done to screen pregnant women for vaginal and rectal GBS colonization during their regular visits to antenatal clinic. Todd-Hewitt broth, an enrichment medium for GBS was used for isolation. The antibiotic susceptibility pattern of the isolates were studied. Results: A total of 300 pregnant women were screened for GBS colonization. GBS colonization rate in our study was 2.3%. The antibiotic susceptibility pattern of the isolates revealed that none of the isolates were resistant to penicillin or clindamycin, while resistance was noted to erythromycin (14.3%) and tetracycline (71.4%).Conclusions: GBS continues to remain sensitive to Penicillin which is the drug of choice for prevention and treatment of GBS. Consistent surveillance of antibiotic sensitivity pattern of GBS as well as for other organisms implicated in new born sepsis and maternal infections is required to formulate guidelines for prevention and treatment.

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Group B streptococcus colonization, antibiotic susceptibility, and serotype distribution among Saudi pregnant women

Infection and Chemotherapy ◽

10.3947/ic.2020.52.1.70 ◽

2020 ◽

Vol 52 (1) ◽

pp. 70

Author(s):

Amr Mohamed Mohamed ◽

Mubashir Ahmad Khan ◽

Aftab Faiz ◽

Jawwad Ahmad ◽

Elsheikh Babiker Khidir ◽

...

Keyword(s):

Pregnant Women ◽

Antibiotic Susceptibility ◽

Group B Streptococcus ◽

Serotype Distribution ◽

Group B

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Group B Streptococcus vaginal colonization in pregnant women living with HIV infection: prevalence and antibiotic susceptibility at HIV referral centers in Lagos, Nigeria

The Journal of Maternal-Fetal & Neonatal Medicine ◽

10.1080/14767058.2021.2015575 ◽

2021 ◽

pp. 1-7

Author(s):

O. Makinde ◽

B. O. Okusanya ◽

G. Osanyin

Keyword(s):

Hiv Infection ◽

Pregnant Women ◽

Antibiotic Susceptibility ◽

Group B Streptococcus ◽

Infection Prevalence ◽

Women Living With Hiv ◽

Vaginal Colonization ◽

Group B ◽

Living With Hiv

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Serotype distribution and antibiotic susceptibility of group B streptococci in pregnant women

Epidemiology and Infection ◽

10.1017/s0950268809991105 ◽

2009 ◽

Vol 138 (7) ◽

pp. 979-981 ◽

Cited By ~ 8

Author(s):

A. DHANOA ◽

R. KARUNAKARAN ◽

S. D. PUTHUCHEARY

Keyword(s):

Pregnant Women ◽

Neonatal Sepsis ◽

Antibiotic Susceptibility ◽

Group B Streptococcus ◽

Prevention Strategies ◽

Serotype Distribution ◽

Group B Streptococci ◽

Birth Canal ◽

Group B

SUMMARYGroup B streptococcus (GBS) is a leading cause of neonatal sepsis and is usually acquired via the woman's birth canal. GBS serotypes isolated from 200 pregnant women were determined. Serotypes V (19%) and VI (17%) were the most frequent followed by serotypes III (12%), Ia (11·5%) and IV (10%); 17% of the strains were non-typable. All isolates were susceptible to penicillin, 96% to erythromycin and 97·5% to clindamycin. The emergence of new GBS serotypes has important implications for vaccine prevention strategies.

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Streptococcus agalactiae colonization and screening approach in high-risk pregnant women in southern Brazil

The Journal of Infection in Developing Countries ◽

10.3855/jidc.12025 ◽

2020 ◽

Vol 14 (04) ◽

pp. 332-340

Author(s):

Jeane Zanini da Rocha ◽

Jéssica Feltraco ◽

Vanessa Radin ◽

Carla Vitola Gonçalves ◽

Pedro Eduardo Almeida da Silva ◽

...

Keyword(s):

High Risk ◽

Pregnant Women ◽

Low Cost ◽

Group B Streptococcus ◽

Neonatal Morbidity ◽

Neonatal Infection ◽

Diagnostic Methods ◽

Antenatal Screening ◽

Group B ◽

The Impact

Introduction: Considering that Group B Streptococcus (GBS) persists as an important cause of neonatal morbidity and mortality, the objective of this study was to evaluate the frequency of maternal colonization by GBS, comparing the culture by the Granada broth with the GeneXpert real-time PCR diagnostic methods and the impact of chemoprophylaxis in high-risk pregnant women. Methodology: A prospective cohort of 110 pregnant women hospitalized for gestational complications was formed and recruited following interview and collection of rectovaginal swabs. Results: The frequency of maternal colonization was 28.2% and statistically associated with Capurro> 37 weeks (p = 0.030) and neonatal infection (p = 0.008). Chemoprophylaxis was offered to 80% of those colonized. Among the pregnant women treated, a fivefold reduction in the rate of prematurity and rate of neonatal infection was observed. The sensitivity was 76.6% and 86.6% in culture and PCR, respectively, with an optimal index of agreement between the methods (K = 0.877). Grenade culture was considered an easy and low-cost method, while GeneXpert presented higher cost and error rate of 10%. However, 23.3% of the pregnant women were diagnosed exclusively by GeneXpert and the results were obtained in two hours. Conclusions: This study showed a significant prevalence of maternal colonization for GBS and that both culture and molecular methods had peculiarities that allow different applicability, with the culture being feasible for antenatal screening and in the hospital for high-risk pregnant women with no sign of imminent delivery and GeneXpert being prioritized for situations of preterm birth.

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The Performance of Strep B Carrot Broth™ versus Latex Agglutination Test for Rapid Detection of Group B Streptococcus Colonization Status and Its Prevalence in Near - Term Pregnant Women

The Egyptian Journal of Medical Microbiology ◽

10.12816/0053806 ◽

2018 ◽

Vol 27 (4) ◽

pp. 7-11

Author(s):

Abdelraouf , Nada S. ◽

Khattab , Mona A. ◽

Atallab , Makram F. ◽

Abd El Rahman , Rehab M.

Keyword(s):

Pregnant Women ◽

Rapid Detection ◽

Group B Streptococcus ◽

Latex Agglutination ◽

Agglutination Test ◽

Latex Agglutination Test ◽

Near Term ◽

Group B ◽

Colonization Status

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Colonization prevalence and antibiotic susceptibility of Group B Streptococcus in pregnant women over a 6-year period in Dongguan, China

PLoS ONE ◽

10.1371/journal.pone.0183083 ◽

2017 ◽

Vol 12 (8) ◽

pp. e0183083 ◽

Cited By ~ 17

Author(s):

Wenjing Ji ◽

Lihua Zhang ◽

Zhusheng Guo ◽

Shujin Xie ◽

Weiqing Yang ◽

...

Keyword(s):

Pregnant Women ◽

Antibiotic Susceptibility ◽

Group B Streptococcus ◽

Group B

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Prevalence, Risk Factors, and Serotype Distribution of Group B Streptococcus Colonization in HIV-Infected Pregnant Women Living in Belgium: A Prospective Cohort Study

Open Forum Infectious Diseases ◽

10.1093/ofid/ofy320 ◽

2018 ◽

Vol 5 (12) ◽

Cited By ~ 2

Author(s):

Nicolas Dauby ◽

Catherine Adler ◽

Veronique Y Miendje Deyi ◽

Rosalie Sacheli ◽

Laurent Busson ◽

...

Keyword(s):

Risk Factors ◽

Pregnant Women ◽

Late Onset ◽

Group B Streptococcus ◽

Neonatal Infection ◽

Colonization Rate ◽

Vaccine Serotypes ◽

Related Data ◽

Significant Difference ◽

Group B

Abstract Background Group B streptococcus (GBS) infection is a leading cause of severe neonatal infection. Maternal GBS carriage during pregnancy is the main risk factor for both early-onset and late-onset GBS disease. High incidence of GBS infection has been reported in HIV-exposed but -uninfected infants (HEU). We aimed to determine the prevalence, characteristics, and risk factors for GBS colonization in HIV-infected and HIV-uninfected pregnant women living in Belgium. Methods Between January 1, 2011, and December 31, 2013, HIV-infected (n = 125) and -uninfected (n = 120) pregnant women had recto-vaginal swabs at 35–37 weeks of gestation and at delivery for GBS detection. Demographic, obstetrical, and HIV infection–related data were prospectively collected. GBS capsular serotyping was performed on a limited number of samples (33 from HIV-infected and 16 from HIV-uninfected pregnant women). Results There was no significant difference in the GBS colonization rate between HIV-infected and -uninfected pregnant women (29.6% vs 24.2%, respectively). HIV-infected women were more frequently colonized by serotype III (36.4% vs 12.5%), and the majority of serotype III strains belonged to the hypervirulent clone ST-17. Exclusively trivalent vaccine serotypes (Ia, Ib, and III) were found in 57.6% and 75% of HIV-infected and -uninfected women, respectively, whereas the hexavalent vaccine serotypes (Ia, Ib, II, III, IV, and V) were found in 97% and 100%, respectively. Conclusions HIV-infected and -uninfected pregnant women living in Belgium have a similar GBS colonization rate. A trend to a higher colonization rate with serotype III was found in HIV-infected women, and those serotype III strains belong predominantly to the hypervirulent clone ST17.

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