scholarly journals Expanded transcriptomic view of strawberry fruit ripening through meta-analysis

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0252685
Author(s):  
Gibum Yi ◽  
Hosub Shin ◽  
Kyeonglim Min ◽  
Eun Jin Lee
Keyword(s):  
Fruit Ripening ◽  
Reference Genome ◽  
Fatty Acid Biosynthesis ◽  
Anthocyanin Biosynthesis ◽  
Meta Analysis ◽  
Strawberry Fruit ◽  
Acid Biosynthesis ◽  
Climacteric Fruit ◽  
Plant Sciences ◽  
Strawberry Fruit Ripening

Strawberry is an important fruit crop and a model for studying non-climacteric fruit ripening. Fruit ripening and senescence influence strawberry fruit quality and postharvest storability, and have been intensively studied. However, genetic and physiological differences among cultivars preclude consensus understanding of these processes. We therefore performed a meta-analysis by mapping existing transcriptome data to the newly published and improved strawberry reference genome and extracted meta-differentially expressed genes (meta-DEGs) from six cultivars to provide an expanded transcriptomic view of strawberry ripening. We identified cultivar-specific transcriptome changes in anthocyanin biosynthesis-related genes and common changes in cell wall degradation, chlorophyll degradation, and starch metabolism-related genes during ripening. We also identified 483 meta-DEGs enriched in gene ontology categories related to photosynthesis and amino acid and fatty acid biosynthesis that had not been revealed in previous studies. We conclude that meta-analysis of existing transcriptome studies can effectively address fundamental questions in plant sciences.

scholarly journals Autophagy Is Required for Strawberry Fruit Ripening

2021 ◽  
Vol 12 ◽  
Author(s):  
José F. Sánchez-Sevilla ◽  
Miguel A. Botella ◽  
Victoriano Valpuesta ◽  
Victoria Sanchez-Vera
Keyword(s):  
Fruit Ripening ◽  
Membrane Vesicle ◽  
Normal Growth ◽  
Cellular Level ◽  
Fragaria X Ananassa ◽  
Strawberry Fruit ◽  
Climacteric Fruit ◽  
Crop Development ◽  
Strawberry Fruit Ripening ◽  
Recycling Pathway

Autophagy is a catabolic and recycling pathway that maintains cellular homeostasis under normal growth and stress conditions. Two major types of autophagy, microautophagy and macroautophagy, have been described in plants. During macroautophagy, cellular content is engulfed by a double-membrane vesicle called autophagosome. This vesicle fuses its outer membrane with the tonoplast and releases the content into the vacuole for degradation. During certain developmental processes, autophagy is enhanced by induction of several autophagy-related genes (ATG genes). Autophagy in crop development has been studied in relation to leaf senescence, seed and reproductive development, and vascular formation. However, its role in fruit ripening has only been partially addressed. Strawberry is an important berry crop, representative of non-climacteric fruit. We have analyzed the occurrence of autophagy in developing and ripening fruits of the cultivated strawberry. Our data show that most ATG genes are conserved in the genome of the cultivated strawberry Fragaria x ananassa and they are differentially expressed along the ripening of the fruit receptacle. ATG8-lipidation analysis proves the presence of two autophagic waves during ripening. In addition, we have confirmed the presence of autophagy at the cellular level by the identification of autophagy-related structures at different stages of the strawberry ripening. Finally, we show that blocking autophagy either biochemically or genetically dramatically affects strawberry growth and ripening. Our data support that autophagy is an active and essential process with different implications during strawberry fruit ripening.

scholarly journals Sucrose Promotes Strawberry Fruit Ripening and Affects Ripening-Related Processes

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Ya Luo ◽  
Yuanxiu Lin ◽  
Fan Mo ◽  
Cong Ge ◽  
Leiyu Jiang ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Anthocyanin Biosynthesis ◽  
Expression Profiles ◽  
Strawberry Fruit ◽  
Signal Molecule ◽  
Total Polyphenol ◽  
Application Timing ◽  
Expression Of Genes ◽  
Nonclimacteric Fruit ◽  
Strawberry Fruit Ripening

Strawberry is a typical nonclimacteric fruit, whose ripening mechanism needs to be further investigated. Sucrose has been recently proved as a signal molecule, participating in strawberry fruit ripening and related processes. While in the effects of sucrose application timing and concentration on ripening, fruit qualities remain unclear, as well as the transcriptome-wide details about the effects of sucrose on the gene expression involved in ripening-related processes. In this study, strawberry fruits at the degreening (DG), white (W), and initial-red (IR) stages were treated with different concentration of sucrose. The results showed that anthocyanin was increased while total polyphenol concentration (TPC) and total flavonoid concentration (TFC) were decreased during fruit development after sucrose treatment. Interestingly, It was showed that 100 mM sucrose application at the DG stage had the most obvious effects on fruit ripening; it made all the fruits turn into full-red (FR) around 4 days (d) earlier than the control, while it did not affect fruit quality traits and most bioactive compounds in the FR fruits. Subsequently, RNA sequencing (RNAseq) of the fruits collected at 8 days after 100 mM sucrose treatment was carried out. It was suggested that 993 genes were differentially expressed comparing with the control. Transcriptome-based expression analysis revealed that sucrose induced the expression of genes involved in the AsA and anthocyanin biosynthesis, while largely suppressed the expression of genes in TCA. The results obtained in this study provided more expression profiles of ripening-related genes under the treatment of sucrose, which will contribute to a better understanding for the mechanism underlying sucrose-induced fruit ripening.

scholarly journals Downregulation of RdDM during strawberry fruit ripening

2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Jingfei Cheng ◽  
Qingfeng Niu ◽  
Bo Zhang ◽  
Kunsong Chen ◽  
Ruihua Yang ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Strawberry Fruit ◽  
Strawberry Fruit Ripening

Melatonin accelerates strawberry fruit ripening by triggering GAMYB gene expression and promoting ABA accumulation

2021 ◽  
Vol 281 ◽  
pp. 109919
Author(s):  
Sirvan Mansouri ◽  
Hassan Sarikhani ◽  
Mohammad Sayyari ◽  
Morteza Soleimani Aghdam
Keyword(s):  
Gene Expression ◽  
Fruit Ripening ◽  
Strawberry Fruit ◽  
Strawberry Fruit Ripening

MAPK5 and MAPK10 overexpression influences strawberry fruit ripening, antioxidant capacity and resistance to Botrytis cinerea

Planta ◽  
2021 ◽  
Vol 255 (1) ◽  
Author(s):  
Yunting Zhang ◽  
Yu Long ◽  
Yiting Liu ◽  
Min Yang ◽  
Liangxin Wang ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Botrytis Cinerea ◽  
Fruit Ripening ◽  
Strawberry Fruit ◽  
Strawberry Fruit Ripening

scholarly journals Ethylene is involved in strawberry fruit ripening in an organ-specific manner

2013 ◽  
Vol 64 (14) ◽  
pp. 4421-4439 ◽  
Author(s):  
Catharina Merchante ◽  
José G. Vallarino ◽  
Sonia Osorio ◽  
Irene Aragüez ◽  
Natalia Villarreal ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Strawberry Fruit ◽  
Specific Manner ◽  
Organ Specific ◽  
Strawberry Fruit Ripening

scholarly journals Brassinosteroid is involved in strawberry fruit ripening

2012 ◽  
Vol 69 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Ye-mao Chai ◽  
Qing Zhang ◽  
Lin Tian ◽  
Chun-Li Li ◽  
Yu Xing ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Strawberry Fruit ◽  
Strawberry Fruit Ripening

scholarly journals The long noncoding RNA FRILAIR regulates strawberry fruit ripening by functioning as a noncanonical target mimic

PLoS Genetics ◽  
2021 ◽  
Vol 17 (3) ◽  
pp. e1009461
Author(s):  
Yajun Tang ◽  
Zhipeng Qu ◽  
Jiajun Lei ◽  
Reqing He ◽  
David L. Adelson ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Noncoding Rna ◽  
Expression Patterns ◽  
Noncoding Rnas ◽  
Mrna Levels ◽  
Strawberry Fruit ◽  
Rna Seq ◽  
Fruit Maturation ◽  
Temporal Expression ◽  
Strawberry Fruit Ripening

Long noncoding RNAs (lncRNAs) are emerging as important regulators in plant development, but few of them have been functionally characterized in fruit ripening. Here, we have identified 25,613 lncRNAs from strawberry ripening fruits based on RNA-seq data from poly(A)-depleted libraries and rRNA-depleted libraries, most of which exhibited distinct temporal expression patterns. A novel lncRNA, FRILAIR harbours the miR397 binding site that is highly conserved in diverse strawberry species. FRILAIR overexpression promoted fruit maturation in the Falandi strawberry, which was consistent with the finding from knocking down miR397, which can guide the mRNA cleavage of both FRILAIR and LAC11a (encoding a putative laccase-11-like protein). Moreover, LAC11a mRNA levels were increased in both FRILAIR overexpressing and miR397 knockdown fruits, and accelerated fruit maturation was also found in LAC11a overexpressing fruits. Overall, our study demonstrates that FRILAIR can act as a noncanonical target mimic of miR397 to modulate the expression of LAC11a in the strawberry fruit ripening process.

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