scholarly journals Fungal Super Glue: The Biofilm Matrix and Its Composition, Assembly, and Functions

2016 ◽  
Vol 12 (9) ◽  
pp. e1005828 ◽  
Author(s):  
Kaitlin F. Mitchell ◽  
Robert Zarnowski ◽  
David R. Andes
Keyword(s):  
Biofilm Matrix ◽  
Super Glue

scholarly journals Anti-Biofilm Molecules Targeting Functional Amyloids

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 795
Author(s):  
Leticia Matilla-Cuenca ◽  
Alejandro Toledo-Arana ◽  
Jaione Valle
Keyword(s):  
Biofilm Formation ◽  
Therapeutic Strategy ◽  
Research Area ◽  
Therapeutic Strategies ◽  
Structural Components ◽  
Significant Issue ◽  
Wide Range ◽  
Effective Therapeutic Strategy ◽  
Functional Amyloids ◽  
Biofilm Matrix

The choice of an effective therapeutic strategy in the treatment of biofilm-related infections is a significant issue. Amyloids, which have been historically related to human diseases, are now considered to be prevailing structural components of the biofilm matrix in a wide range of bacteria. This assumption creates the potential for an exciting research area, in which functional amyloids are considered to be attractive targets for drug development to dissemble biofilm structures. The present review describes the best-characterized bacterial functional amyloids and focuses on anti-biofilm agents that target intrinsic and facultative amyloids. This study provides a better understanding of the different modes of actions of the anti-amyloid molecules to inhibit biofilm formation. This information can be further exploited to improve the therapeutic strategies to combat biofilm-related infections.

scholarly journals Methods to extract the exopolymeric matrix from biofilms: a comparative study

1999 ◽  
Vol 39 (7) ◽  
pp. 243-250 ◽  
Author(s):  
Joana Azeredo ◽  
Valentina Lazarova ◽  
Rosário Oliveira
Keyword(s):  
Organic Carbon ◽  
Comparative Study ◽  
Mixed Culture ◽  
Pure Culture ◽  
Cell Walls ◽  
Extraction Method ◽  
Extraction Methods ◽  
Suitable Method ◽  
The Matrix ◽  
Biofilm Matrix

To study the composition of a biofilm a previous extraction method is required to separate cells from the matrix. There are several methods reported in the literature; however they are not efficient or promote leakage of intracellular material. In this work several extraction methods were assayed in mixed culture and pure culture biofilms and their efficiency was evaluated by the amount of organic carbon, proteins and intracellular material extracted. The results showed that the extraction with glutaraldehyde 3% (w/v) was the most suitable method, extracting great amounts of organic carbon without promoting cell lysis or permeabilization. Glutaraldehyde is a bifunctional reagent that binds to cell walls avoiding their permeabilization and the biofilm matrix is solubilized in the solution.

The biofilm matrix

2010 ◽  
Vol 8 (9) ◽  
pp. 623-633 ◽  
Author(s):  
Hans-Curt Flemming ◽  
Jost Wingender
Keyword(s):  
Biofilm Matrix

scholarly journals Effect of endodontic irrigants on biofilm matrix polysaccharides

2016 ◽  
Vol 50 (2) ◽  
pp. 153-160 ◽  
Author(s):  
P. N. Tawakoli ◽  
K. T. Ragnarsson ◽  
D. K. Rechenberg ◽  
D. Mohn ◽  
M. Zehnder
Keyword(s):  
Biofilm Matrix

scholarly journals Biofilm matrix and artificial mediator for efficient electron transport in CO2 microbial electrosynthesis

2021 ◽  
pp. 131885
Author(s):  
Young Eun Song ◽  
Abdelrhman Mohamed ◽  
Changman Kim ◽  
Minsoo Kim ◽  
Shuwei Li ◽  
...  
Keyword(s):  
Electron Transport ◽  
Microbial Electrosynthesis ◽  
Biofilm Matrix

Spilanthol as a promising antifungal alkylamide for the treatment of vulvovaginal candidiasis

2021 ◽  
Author(s):  
Rodrigo L Fabri ◽  
Jhamine C O Freitas ◽  
Ari S O Lemos ◽  
Lara M Campos ◽  
Irley O M Diniz ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Cell Membrane ◽  
Multidrug Resistant ◽  
Fungal Strain ◽  
Vulvovaginal Candidiasis ◽  
Biofilm Matrix

Abstract Spilanthol is a bioactive alkylamide from the native Amazon plant species, Acmella oleracea. However, antifungal activities of spilanthol and its application to the therapeutic treatment of candidiasis remains to be explored. This study sought to evaluate the in vitro and in vivo antifungal activity of spilanthol previously isolated from A. oleracea (spilanthol(AcO)) against Candida albicans ATCC® 10231™, a multidrug-resistant fungal strain. Microdilution methods were used to determine inhibitory and fungicidal concentrations of spilanthol(AcO). In planktonic cultures, the fungal growth kinetics, yeast cell metabolic activity, cell membrane permeability and cell wall integrity were investigated. The effect of spilanthol(AcO) on the proliferation and adhesion of fungal biofilms was evaluated by whole slide imaging and scanning electron microscopy. The biochemical composition of the biofilm matrix was also analyzed. In parallel, spilanthol(AcO) was tested in vivo in an experimental vulvovaginal candidiasis model. Our in vitro analyses in C. albicans planktonic cultures detected a significant inhibitory effect of spilanthol(AcO), which affects both yeast cell membrane and cell wall integrity, interfering with the fungus growth. C. albicans biofilm proliferation and adhesion, as well as, carbohydrates and DNA in biofilm matrix were reduced after spilanthol(AcO) treatment. Moreover, infected rats treated with spilanthol(AcO) showed consistent reduction of both fungal burden and inflammatory processes compared to the untreated animals. Altogether, our findings demonstrated that spilanthol(AcO) is an bioactive compound against planktonic and biofilm forms of a multidrug resistant C. albicans strain. Furthermore, spilanthol(AcO) can be potentially considered for therapeutical treatment of vulvovaginal candidiasis caused by C. albicans. Lay Abstract This study sought to evaluate the antifungal activity of spilanthol against Candida albicans ATCC® 10 231™, a multidrug-resistant fungal strain. Our findings demonstrated that spilanthol(AcO) can be potentially considered for therapeutical treatment of vulvovaginal candidiasis caused by C. albicans.

scholarly journals Study of the Response of a Biofilm Bacterial Community to UV Radiation

1999 ◽  
Vol 65 (5) ◽  
pp. 2025-2031 ◽  
Author(s):  
Mohamed O. Elasri ◽  
Robert V. Miller
Keyword(s):  
Uv Radiation ◽  
Protective Property ◽  
Uv Light ◽  
Planktonic Cells ◽  
Light Production ◽  
Whole Cell Biosensor ◽  
Uv C ◽  
Cell Biosensor ◽  
Biofilm Matrix ◽  
Alginate Matrix

ABSTRACT We have developed a bioluminescent whole-cell biosensor that can be incorporated into biofilm ecosystems. RM4440 is a Pseudomonas aeruginosa FRD1 derivative that carries a plasmid-basedrecA-luxCDABE fusion. We immobilized RM4440 in an alginate matrix to simulate a biofilm, and we studied its response to UV radiation damage. The biofilm showed a protective property by physical shielding against UV C, UV B, and UV A. Absorption of UV light by the alginate matrix translated into a higher survival rate than observed with planktonic cells at similar input fluences. UV A was shown to be effectively blocked by the biofilm matrix and to have no detectable effects on cells contained in the biofilm. However, in the presence of photosensitizers (i.e., psoralen), UV A was effective in inducing light production and cell death. RM4440 has proved to be a useful tool to study microbial communities in a noninvasive manner.

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